scholarly journals Predicting complex phenotype–genotype interactions to enable yeast engineering: Saccharomyces cerevisiae as a model organism and a cell factory

2013 ◽  
Vol 8 (9) ◽  
pp. 1017-1034 ◽  
Author(s):  
Duygu Dikicioglu ◽  
Pınar Pir ◽  
Stephen G. Oliver
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Model Organism ◽  
Cell Factory ◽  
Complex Phenotype ◽  
A Cell

scholarly journals Leveraging budding yeast Saccharomyces cerevisiae for discovering aging modulation substances for functional food

2019 ◽  
Vol 9 (5) ◽  
pp. 297
Author(s):  
Shaoyu Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Bioactive Compounds ◽  
Functional Food ◽  
Budding Yeast ◽  
Model Organism ◽  
Cellular Systems ◽  
Cell Factory ◽  
Compression Of Morbidity ◽  
Bioactive Substances ◽  
Yeast Saccharomyces Cerevisiae

Background: Discovery of bioactive substances contained in functional food and the mechanism of their aging modulation are imperative steps in developing better, potent and safer functional food for promoting health and compression of morbidity in the aging population.  Budding yeast (Saccharomyces cerevisiae) is invaluable model organism for aging modulation and bioactive compounds discovery. In this paper we have conceptualised a framework for achieving such aim. This framework consists of four components: discovering targets for aging modulation, discovering and validating caloric restriction mimetics, acting as cellular systems for screening natural products or compounds for aging modulation and being a biological factory for producing bioactive compounds according to the roles the yeast systems play. It have been argued that the component of being a biological factory for producing bioactive compounds has much underexplored which also present an opportunity for new active substance discovery and validation for health promotion in functional food industry.Keywords: Aging modulation, budding yeast, functional food, bioactive substances, cell factory

scholarly journals The Plasma Membrane at the Cornerstone Between Flexibility and Adaptability: Implications for Saccharomyces cerevisiae as a Cell Factory

2021 ◽  
Vol 12 ◽  
Author(s):  
Luís Ferraz ◽  
Michael Sauer ◽  
Maria João Sousa ◽  
Paola Branduardi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Cell Factory ◽  
Membrane Composition ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
A Cell ◽  
Biotechnological Processes ◽  
Membrane Engineering ◽  
The Impact

In the last decade, microbial-based biotechnological processes are paving the way toward sustainability as they implemented the use of renewable feedstocks. Nonetheless, the viability and competitiveness of these processes are often limited due to harsh conditions such as: the presence of feedstock-derived inhibitors including weak acids, non-uniform nature of the substrates, osmotic pressure, high temperature, extreme pH. These factors are detrimental for microbial cell factories as a whole, but more specifically the impact on the cell’s membrane is often overlooked. The plasma membrane is a complex system involved in major biological processes, including establishing and maintaining transmembrane gradients, controlling uptake and secretion, intercellular and intracellular communication, cell to cell recognition and cell’s physical protection. Therefore, when designing strategies for the development of versatile, robust and efficient cell factories ready to tackle the harshness of industrial processes while delivering high values of yield, titer and productivity, the plasma membrane has to be considered. Plasma membrane composition comprises diverse macromolecules and it is not constant, as cells adapt it according to the surrounding environment. Remarkably, membrane-specific traits are emerging properties of the system and therefore it is not trivial to predict which membrane composition is advantageous under certain conditions. This review includes an overview of membrane engineering strategies applied to Saccharomyces cerevisiae to enhance its fitness under industrially relevant conditions as well as strategies to increase microbial production of the metabolites of interest.

Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase

Fuel ◽  
2022 ◽  
Vol 313 ◽  
pp. 122986
Author(s):  
Archontoula Kalogeropoulou ◽  
Iris Plioni ◽  
Dimitra Dimitrellou ◽  
Magdalini Soupioni ◽  
Poonam Singh Nigam ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Factory ◽  
A Cell ◽  
Starch Gel ◽  
Fuel Grade Ethanol

scholarly journals Genome sequencing of the neotype strain CBS 554.65 reveals the MAT1–2 locus of Aspergillus niger

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Valeria Ellena ◽  
Sjoerd J. Seekles ◽  
Gabriel A. Vignolle ◽  
Arthur F. J. Ram ◽  
Matthias G. Steiger
Keyword(s):  
Aspergillus Niger ◽  
Genome Sequence ◽  
Model Organism ◽  
Nuclear Genome ◽  
Cell Factory ◽  
Dna Assembly ◽  
High Quality ◽  
Wide Range ◽  
Conserved Genes ◽  
A Cell

Abstract Background Aspergillus niger is a ubiquitous filamentous fungus widely employed as a cell factory thanks to its abilities to produce a wide range of organic acids and enzymes. Its genome was one of the first Aspergillus genomes to be sequenced in 2007, due to its economic importance and its role as model organism to study fungal fermentation. Nowadays, the genome sequences of more than 20 A. niger strains are available. These, however, do not include the neotype strain CBS 554.65. Results The genome of CBS 554.65 was sequenced with PacBio. A high-quality nuclear genome sequence consisting of 17 contigs with a N50 value of 4.07 Mbp was obtained. The assembly covered all the 8 centromeric regions of the chromosomes. In addition, a complete circular mitochondrial DNA assembly was obtained. Bioinformatic analyses revealed the presence of a MAT1-2-1 gene in this genome, contrary to the most commonly used A. niger strains, such as ATCC 1015 and CBS 513.88, which contain a MAT1-1-1 gene. A nucleotide alignment showed a different orientation of the MAT1–1 locus of ATCC 1015 compared to the MAT1–2 locus of CBS 554.65, relative to conserved genes flanking the MAT locus. Within 24 newly sequenced isolates of A. niger half of them had a MAT1–1 locus and the other half a MAT1–2 locus. The genomic organization of the MAT1–2 locus in CBS 554.65 is similar to other Aspergillus species. In contrast, the region comprising the MAT1–1 locus is flipped in all sequenced strains of A. niger. Conclusions This study, besides providing a high-quality genome sequence of an important A. niger strain, suggests the occurrence of genetic flipping or switching events at the MAT1–1 locus of A. niger. These results provide new insights in the mating system of A. niger and could contribute to the investigation and potential discovery of sexuality in this species long thought to be asexual.

scholarly journals In Vivo Production of RNA Aptamers and Nanoparticles: Problems and Prospects

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1422
Author(s):  
Ousama Al Shanaa ◽  
Andrey Rumyantsev ◽  
Elena Sambuk ◽  
Marina Padkina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Large Scale ◽  
Potential Model ◽  
Model Organism ◽  
Rna Aptamers ◽  
Early Stages ◽  
Near Future

RNA aptamers are becoming increasingly attractive due to their superior properties. This review discusses the early stages of aptamer research, the main developments in this area, and the latest technologies being developed. The review also highlights the advantages of RNA aptamers in comparison to antibodies, considering the great potential of RNA aptamers and their applications in the near future. In addition, it is shown how RNA aptamers can form endless 3-D structures, giving rise to various structural and functional possibilities. Special attention is paid to the Mango, Spinach and Broccoli fluorescent RNA aptamers, and the advantages of split RNA aptamers are discussed. The review focuses on the importance of creating a platform for the synthesis of RNA nanoparticles in vivo and examines yeast, namely Saccharomyces cerevisiae, as a potential model organism for the production of RNA nanoparticles on a large scale.

scholarly journals Non-Ionizing Millimeter Waves Non-Thermal Radiation of Saccharomyces cerevisiae—Insights and Interactions

2021 ◽  
Vol 11 (14) ◽  
pp. 6635
Author(s):  
Ayan Barbora ◽  
Shailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Millimeter Waves ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genetic Alterations ◽  
Power Delivery ◽  
Repair Gene ◽  
Safety Standards

Non-ionizing millimeter-waves (MMW) interact with cells in a variety of ways. Here the inhibited cell division effect was investigated using 85–105 GHz MMW irradiation within the International Commission on Non-Ionizing Radiation Protection (ICNIRP) non-thermal 20 mW/cm2 safety standards. Irradiation using a power density of about 1.0 mW/cm2 SAR over 5–6 h on 50 cells/μL samples of Saccharomyces cerevisiae model organism resulted in 62% growth rate reduction compared to the control (sham). The effect was specific for 85–105 GHz range and was energy- and cell density-dependent. Irradiation of wild type and Δrad52 (DNA damage repair gene) deleted cells presented no differences of colony growth profiles indicating non-thermal MMW treatment does not cause permanent genetic alterations. Dose versus response relations studied using a standard horn antenna (~1.0 mW/cm2) and compared to that of a compact waveguide (17.17 mW/cm2) for increased power delivery resulted in complete termination of cell division via non-thermal processes supported by temperature rise measurements. We have shown that non-thermal MMW radiation has potential for future use in treatment of yeast related diseases and other targeted biomedical outcomes.

scholarly journals Generation of catalytically active 6-phosphofructokinase from Saccharomyces cerevisiae in a cell-free system

2000 ◽  
Vol 267 (15) ◽  
pp. 4825-4830 ◽  
Author(s):  
Anke Edelmann ◽  
Jürgen Kirchberger ◽  
Manfred Naumann ◽  
Gerhard Kopperschläger
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Free System ◽  
Cell Free System ◽  
Catalytically Active ◽  
A Cell
Sign in / Sign up

Export Citation Format

Share Document