Introducing a delivery system for melanogenesis inhibition in melanoma B16F10 cells mediated by the conjugation of tyrosine ammonia‐lyase and a TAT ‐penetrating peptide

2020 ◽  
Author(s):  
Yasaman Behzadipour ◽  
Issa Sadeghian ◽  
Ali Ghaffarian Bahraman ◽  
Shiva Hemmati
Keyword(s):  
Delivery System ◽  
B16f10 Cells ◽  
Tyrosine Ammonia Lyase ◽  
Melanoma B16f10

scholarly journals Unveiling the Differential Antioxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment with Hydrogen Peroxide

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4020
Author(s):  
Khalida Mokhtari ◽  
Amalia Pérez-Jiménez ◽  
Leticia García-Salguero ◽  
José A. Lupiáñez ◽  
Eva E. Rufino-Palomares
Keyword(s):  
Antioxidant Enzyme ◽  
Cell Lines ◽  
Enzyme Activities ◽  
Biological Properties ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Glutathione S Transferase ◽  
Maslinic Acid ◽  
B16f10 Cells ◽  
Melanoma B16f10

Maslinic acid (MA) is a natural triterpene from Olea europaea L. with multiple biological properties. The aim of the present study was to examine MA’s effect on cell viability (by the MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key antioxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to those on healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM), whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM H2O2 was added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, which were higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in antioxidant enzyme activities depending on the cell line. In A10 cells, all the enzymes were up-regulated, but in B16F10 cells, only superoxide dismutase, glutathione S-transferase and glutathione peroxidase increased their activities. MA restored the enzyme activities to levels similar to those in the control group in both cell lines, highlighting that in A10 cells, the highest MA doses induced values lower than control. Overall, these findings demonstrate the great antioxidant capacity of MA.

Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs

2014 ◽  
Vol 38 (4) ◽  
pp. 452-461 ◽  
Author(s):  
Jun Dou ◽  
Xiangfeng He ◽  
Yurong Liu ◽  
Yaqian Wang ◽  
Fengshu Zhao ◽  
...  
Keyword(s):  
Vimentin Expression ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Cynthia Aracely Alvizo Báez ◽  
Itza Eloisa Luna Cruz ◽  
Maria Cristina Rodríguez Padilla ◽  
Juan Manuel Alcocer González
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Delivery System ◽  
Targeted Delivery ◽  
Retention Rate ◽  
Chitosan Nanoparticles ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Intratracheal Administration ◽  
B16f10 Cells

One of the major problems of gene therapy is the efficient, specific, and targeted delivery as well as the safety of the materials used in such systems. The specific targeted delivery of genes to the lung offers the possibility to treat a variety of specific diseases. We developed chitosan nanoparticles with the plasmid pCEM-Luc, which contains a promoter activated by magnetic field. Nanoparticles of 200–250 nm obtained by ionic gelation with a 99% retention rate were transfected in B16F10 cells andin vivoin the lungs of Balb/c mice by intratracheal administration. We observed that an external magnetic field increased the expression of the luciferase reporter gene in B16F10 cells transfected with magnetic nanoparticles and in homogenized lungs of mice which determined differences in levels of expression between different regions of the lungs (apical or distal and left or right). The highest levels of luciferase activity were observed in the apical left region. The magnetic nanoparticles prove an efficient delivery system toin vitrotransfection of cells and lung tissue.

scholarly journals Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Caixia Gao ◽  
Xinyan Yan ◽  
Bo Wang ◽  
Lina Yu ◽  
Jichun Han ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Mouse Melanoma ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Self-Assembled, Adjuvant/Antigen-Based Nanovaccine Mediates Anti-Tumor Immune Response Against Melanoma Tumor

2018 ◽  
Author(s):  
Santhosh Kalash Rajendrakumar ◽  
Adityanarayan Mohapatra ◽  
Bijay Singh ◽  
Chong-Su Cho ◽  
In-Kyu Park
Keyword(s):  
Immune Response ◽  
Clinical Trials ◽  
Delivery System ◽  
Treatment Strategies ◽  
Xenograft Model ◽  
Poly I:C ◽  
Tumor Xenograft ◽  
Preclinical Research ◽  
Melanoma Tumor ◽  
B16f10 Cells

Malignant melanoma is a highly aggressive type of cancer that requires radical treatment strategies to inhibit the cancer cell progression and metastasis. In recent years, preclinical research and clinical trials on melanoma treatment are considerably focused on the adjuvant-based immunotherapy for enhancing the immune response of innate immune cells against cancer cells. However, the clinical outcome of these adjuvant-based treatments are inadequate due to improper delivery system for these immune activators to reach the target site. Hence, we developed a vaccine formulation containing tumor lysate protein (TL) and poly I:C (PIC) complexed with positively charged poly (sorbitol-co- polyethylenimine (PEI)(PSPEI). The resulting ionic PSPEI-polyplexed antigen/adjuvant (PAA) (PSPEI-PAA) nanocomplexes were stable at the physiological condition, non-toxic and  enhanced intracellular uptake in immature dendritic cells. In murine B16F10 tumor xenograft model, PSPEI-PAA nanocomplexes significantly suppressed tumor growth and did not exhibit any noticeable sign of toxicity. Additionally, the cytotoxic T lymphocytes (CTLs) assay involving co-culturing of splenocytes isolated from the PSPEI-PAA-treated mice with that of B16F10 cells significantly revealed enhanced cancer killing by the TL-reactivated CTLs compared to untreated control mice bearing tumor. Therefore, we strongly believe that PSPEI-PAA nanocomplexes could be an efficient antigen/adjuvant delivery system and also enhance the antitumor immune response against melanoma tumor in the future clinical trials.

scholarly journals Anti-metastatic Effect of Garlic Hexane Extract on Lung Metastasis Induced by Melanoma B16F10 Cells in Mice

2016 ◽  
Vol 26 (2) ◽  
pp. 259-264
Author(s):  
Min Jung Ko ◽  
Seetharaman Rajasekar ◽  
Ziyu Wang ◽  
Mei Li ◽  
Jung Ho Kwak ◽  
...  
Keyword(s):  
Lung Metastasis ◽  
Hexane Extract ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Graphene-Induced Hyperthermia (GIHT) Combined With Radiotherapy Fosters Immunogenic Cell Death

2021 ◽  
Vol 11 ◽  
Author(s):  
Malgorzata J. Podolska ◽  
Xiaomei Shan ◽  
Christina Janko ◽  
Rabah Boukherroub ◽  
Udo S. Gaipl ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Combined Treatment ◽  
Infrared Light ◽  
Immunogenic Cell Death ◽  
Tumor Cell Death ◽  
Induced Hyperthermia ◽  
B16f10 Cells ◽  
Melanoma B16f10

Radiotherapy and chemotherapy are the standard interventions for cancer patients, although cancer cells often develop radio- and/or chemoresistance. Hyperthermia reduces tumor resistance and induces immune responses resulting in a better prognosis. We have previously described a method to induce tumor cell death by local hyperthermia employing pegylated reduced graphene oxide nanosheets and near infrared light (graphene-induced hyperthermia, GIHT). The spatiotemporal exposure/release of heat shock proteins (HSP), high group mobility box 1 protein (HMGB1), and adenosine triphosphate (ATP) are reported key inducers of immunogenic cell death (ICD). We hypothesize that GIHT decisively contributes to induce ICD in irradiated melanoma B16F10 cells, especially in combination with radiotherapy. Therefore, we investigated the immunogenicity of GIHT alone or in combination with radiotherapy in melanoma B16F10 cells. Tumor cell death in vitro revealed features of apoptosis that is progressing fast into secondary necrosis. Both HSP70 and HMGB1/DNA complexes were detected 18 hours post GIHT treatment, whereas the simultaneous release of ATP and HMGB1/DNA was observed only 24 hours post combined treatment. We further confirmed the adjuvant potential of these released DAMPs by immunization/challenge experiments. The inoculation of supernatants of cells exposed to sole GIHT resulted in tumor growth at the site of inoculation. The immunization with cells exposed to sole radiotherapy rather fostered the growth of secondary tumors in vivo. Contrarily, a discreet reduction of secondary tumor volumes was observed in mice immunized with a single dose of cells and supernatants treated with the combination of GIHT and irradiation. We propose the simultaneous release of several DAMPs as a potential mechanism fostering anti-tumor immunity against previously irradiated cancer cells.

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