On the impact of sample size on median lethal concentration estimation in acute fish toxicity testing: Is n  = 7/group enough?

2018 ◽  
Vol 37 (6) ◽  
pp. 1565-1578 ◽  
Author(s):  
Gregory J. Carr ◽  
A. John Bailer ◽  
Jane M. Rawlings ◽  
Scott E. Belanger
Our Nature ◽  
1970 ◽  
Vol 8 (1) ◽  
pp. 180-184 ◽  
Author(s):  
P.R. Bhamre ◽  
S.P. Thorat ◽  
A.E. Desai ◽  
B.M. Deoray

Acute toxicity testing of fresh water mussel Lamellidens consobrinus to HgCl2, CdCl2 and ZnSO4 was carried out. The median lethal concentration (LC50) of HgCl2, CdCl2 and ZnSO4 for 24, 48, 72 and 96 hrs were 1.9616, 1.8602, 1.6983 and 1.4066; 1.9050, 1.8631, 1.7542 and 1.6195; 1.9952, 1.9664, 1.9529 and 1.6756 ppm respectively. Analysis of results indicates that L. consobrinus is highly sensitive to HgCl2 than CdCl2 and ZnSO4. The toxicants can be arranged in order of their toxicities as Hg>Cd>Zn.DOI: 10.3126/on.v8i1.4326


2020 ◽  
Vol 8 (2) ◽  
pp. 147-152
Author(s):  
Maria Magdalena Kewa ◽  
Julianty Almet ◽  
Meity Marviana Laut

The Culex mosquito is a species that can creates health problems for humans and animals. The handling efforts of the vector is very important to reduce the impact caused by this vector. One of the plants that has the potential as a larvicide is soursop leaves (Annona muricata L.). This study aims to determine the effect of soursop leaves (Annona muricata L.) extract on the mortality of Culex sp larvae and LC50 value to killing 50% Culex sp larva. The study was conducted from March to June 2020. This study used 7 treatment groups which 5 groups tested the effectiveness of soursop leaves extract and 2 control groups. The research data was analyzed using the Probit test to determine the LC50. The results showed that soursop leaves extract (Annona muricata L.) was effective in killing Culex sp larvae with LC50 value is 0.736%.


2020 ◽  
Author(s):  
Qing Zhao ◽  
Pei Chen ◽  
Yu Zhang ◽  
Haining Liu ◽  
Xianwen Li

BACKGROUND Mobile health application has become an important tool for healthcare systems. One such tool is the delivery of assisting in people with cognitive impairment and their caregivers. OBJECTIVE This scoping review aims to explore and evaluate the existing evidence and challenges on the use of mHealth applications that assisting in people with cognitive impairment and their caregivers. METHODS Nine databases, including PubMed, EMBASE, Cochrane, PsycARTICLES, CINAHL, Web of Science, Applied Science & Technology Source, IEEE Xplore and the ACM Digital Library were searched from inception through June 2020 for the studies of mHealth applications on people with cognitive impairment and their caregivers. Two reviewers independently extracted, checked synthesized data independently. RESULTS Of the 6101 studies retrieved, 64 studies met the inclusion criteria. Three categories emerged from this scoping review. These categories are ‘application functionality’, ‘evaluation strategies’, ‘barriers and challenges’. All the included studies were categorized into 7 groups based on functionality: (1) cognitive assessment; (2) cognitive training; (3) life support; (4) caregiver support; (5) symptom management; (6) reminiscence therapy; (7) exercise intervention. The included studies were broadly categorized into four types: (1) Usability testing; (2) Pilot and feasibility studies; (3) Validation studies; and (4) Efficacy or Effectiveness design. These studies had many defects in research design such as: (1) small sample size; (2) deficiency in active control group; (3) deficiency in analyzing the effectiveness of intervention components; (4) lack of adverse reactions and economic evaluation; (5) lack of consideration about the education level, electronic health literacy and smartphone proficiency of the participants; (6) deficiency in assessment tool; (7) lack of rating the quality of mHealth application. Some progress should be improved in the design of smartphone application functionality, such as: (1) the design of cognitive measurements and training game need to be differentiated; (2) reduce the impact of the learning effect. Besides this, few studies used health behavior theory and performed with standardized reporting. CONCLUSIONS Preliminary results show that mobile technologies facilitate the assistance in people with cognitive impairment and their caregivers. The majority of mHealth application interventions incorporated usability outcome and health outcomes. However, these studies have many defects in research design that limit the extrapolation of research. The content of mHealth application is urgently improved to adapt to demonstrate the real effect. In addition, further research with strong methodological rigor and adequate sample size are needed to examine the feasibility, effectiveness, and cost-effectiveness of mHealth applications for people with cognitive impairment and their caregivers.


Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1929
Author(s):  
Tereza Cervena ◽  
Andrea Rossnerova ◽  
Tana Zavodna ◽  
Jitka Sikorova ◽  
Kristyna Vrbova ◽  
...  

The evaluation of the frequency of micronuclei (MN) is a broadly utilised approach in in vitro toxicity testing. Nevertheless, the specific properties of nanomaterials (NMs) give rise to concerns regarding the optimal methodological variants of the MN assay. In bronchial epithelial cells (BEAS-2B), we tested the genotoxicity of five types of NMs (TiO2: NM101, NM103; SiO2: NM200; Ag: NM300K, NM302) using four variants of MN protocols, differing in the time of exposure and the application of cytochalasin-B combined with the simultaneous and delayed co-treatment with NMs. Using transmission electron microscopy, we evaluated the impact of cytochalasin-B on the transport of NMs into the cells. To assess the behaviour of NMs in a culture media for individual testing conditions, we used dynamic light scattering measurement. The presence of NMs in the cells, their intracellular aggregation and dispersion properties were comparable when tests with or without cytochalasin-B were performed. The genotoxic potential of various TiO2 and Ag particles differed (NM101 < NM103 and NM302 < NM300K, respectively). The application of cytochalasin-B tended to increase the percentage of aberrant cells. In conclusion, the comparison of the testing strategies revealed that the level of DNA damage induced by NMs is affected by the selected methodological approach. This fact should be considered in the interpretation of the results of genotoxicity tests.


2016 ◽  
Vol 76 ◽  
pp. 231-233 ◽  
Author(s):  
Thomas H. Hutchinson ◽  
James R. Wheeler ◽  
Anne Gourmelon ◽  
Natalie Burden

2021 ◽  
Vol 5 (1) ◽  
pp. 61
Author(s):  
Rachid Laref ◽  
Etienne Losson ◽  
Alexandre Sava ◽  
Maryam Siadat

Low-cost gas sensors detect pollutants gas at the parts-per-billion level and may be installed in small devices to densify air quality monitoring networks for the spread analysis of pollutants around an emissive source. However, these sensors suffer from several issues such as the impact of environmental factors and cross-interfering gases. For instance, the ozone (O3) electrochemical sensor senses nitrogen dioxide (NO2) and O3 simultaneously without discrimination. Alphasense proposes the use of a pair of sensors; the first one, NO2-B43F, is equipped with a filter dedicated to measure NO2. The second one, OX-B431, is sensitive to both NO2 and O3. Thus, O3 concentration can be obtained by subtracting the concentration of NO2 from the sum of the two concentrations. This technique is not practical and requires calibrating each sensor individually, leading to biased concentration estimation. In this paper, we propose Partial Least Square regression (PLS) to build a calibration model including both sensors’ responses and also temperature and humidity variations. The results obtained from data collected in the field for two months show that PLS regression provides better gas concentration estimation in terms of accuracy than calibrating each sensor individually.


2021 ◽  
Vol 66 (18) ◽  
pp. 185012
Author(s):  
Yingtao Fang ◽  
Jiazhou Wang ◽  
Xiaomin Ou ◽  
Hongmei Ying ◽  
Chaosu Hu ◽  
...  

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