The anatomy of males of Nygolaimus aquaticus Thorne, 1930, collected from Blackburn, Ontario, is described. Descriptions are based on whole mounts and also on serial sections. Specimens embedded in paraffin were cut in sections 9 μ in thickness and stained with haematoxylin and eosin.Special emphasis is given to cuticular structure, such as median pores, lateral pores, hemizonid, and transverse and longitudinal striations, which are described and illustrated. Besides these structures, other organ systems of the body, such as digestive system, excretory system, nervous system, and reproductive organs, have also been described and illustrated.