Morbidity, Clinical Course and Vaccination against SARS-CoV-2 Virus in Patients with Duchenne Muscular Dystrophy: A Patient Reported Survey

Background: Patients with Duchenne muscular dystrophy (DMD) may be at higher risk of a severe course of COVID-19. The aim of the study was to evaluate: (1) the incidence and course of COVID-19 infection in DMD patients; (2) the vaccination status of DMD patients; and (3) COVID-19 related anxiety among DMD families. Materials and Methods: The study was conducted during an online symposium for DMD patients and their families. All participants (DMD families; n = 150) were asked to fill in the online survey with questions about COVID-19 infection history, vaccination against SARS-CoV-2 and anxiety during pandemic. Results: 53 DMD patients filled in the survey. Five (9.43%) were COVID-19 positive with mild symptoms of respiratory infection and anosmia; 23 (42.6%) were vaccinated, but in almost 20% of DMD families, none of the family members was vaccinated. Respondents revealed anxiety related both to the vaccination procedure and to COVID-19 infection (complications after infection 93.6%, death 62.4% respondents). Changes in health care system organization also aroused concern among participants (85.3%). Conclusion: The course of the COVID-19 infection in DMD patients was mild. Not enough patients with DMD and their families are vaccinated. Education about the management of COVID-19 infections and the vaccination procedure for DMD patients is needed and expected.

GEODYNAMICS

The article studies the system organization of inversion tectonic deformations of the Dnieper-Donetsk Basin which covered the territory of the Western Donetsk Graben. The research uses the kinematic and structural-paragenetic analysis of inversion structural transformation of the folded floors of the sedimentary cover of the Graben. The original model of tectonic inversion of the Dnieper-Donets Basin was completed from the previous models. The tectonic inversion of the Dnieper-Donets Paleorift rift-like structures began at the late Hercynian stage in the geodynamic environment of the territory of the Eastern European Platform general collision. Tectonophysical analysis shows that the inversion folding was formed by the mechanism of sedimentary horizons longitudinal bending in the environment of the interference of the intraplate submeridional collision compression and the regional strike-slip stress field. At the Mesozoic-Cenozoic stage, tectonic inversion continued in the field of right-hand strike-slip deformations with a variable compressive component. This caused the formation of folded covers of tectonic plates and scales in the uplift-thrust mode. They, Hercynian neo-autochthonous formations and further the weakly located syneclise autochthon of the South-east of the Basin. The pressure of the "tectonic stamp" geoblock of the Donetsk Foldbelt contributed to the formationof the Segment body of geomass Tectonic Wedging. It was diagnosed with a structural orocline of transverse extension of the sliding type. Large linear throw-folded zones were formed within geodynamic bands of injection and displacement of geomass along the front of the orocline. The tectonic compression fan, characteristic of geodynamic compression zones, was formed in the foreland of the orocline, on the ends of the main thrusts. They served as “tectonic rails”of the allochthon invasion within the rift-like structure. There are the transverse zones of tectonic sutures formed on the roots of the folding covers of the Hercynian neo-autochthon thrusting, which are located in the hinterland of the orocline in the Foldbelt Western slope. The study completed an original kinematic model of tectonic inversion of the transition zone between the Dnieper-Donets Basin and Donets Foldbelt. According to the model, the pressure of the “tectonic stamp” geoblock initiated the invasion of the Segment of Tectonic Wedging which consists of the intensively dislocated allochthonous geomass. The Segment destroyed the rift-like structure and formed the Western Donetsk Cover-Folded Region in the South-eastern part of the Basin. The system organization model of inversion complications of the rift-like structure in the territory of the Western Donetsk Graben will allow to improve the regional geological schemes of tectonic oil and gas zoning.

STRUCTURAL AND SEMANTIC ORGANIZATION OF CONCESSIVE RELATIONS IN THE ТATAR LANGUAGE

In modern linguistics there is a growing scientific interest in the semantic analysis of linguistic phenomena. The increased interest is explained by the fact that it is the study of semantic relations between syntactic units that allows us to reveal the communicative essence of the language. In this regard the study of the structural and semantic features of constructions expressing the relations of concession, which are the most complex among other types of conditionality relations, seems relevant and timely. The purpose of this article is to analyze the concessive relations from the formal and substantive side on the material of the Tatar language. The research was carried out using the conceptual apparatus of modern theories of language categories, as well as ideas about the role and functions of concessive relations both in the system organization of language itself and in contextual discourse. The material for the study was drawn from Tatar fiction and colloquial speech. In the course of the work, the method of linguistic description was used as the main research method. As a result of the conducted research, it was found that concessive relations are built on the opposition of two situations, formed on the basis of conditional, causal, and adversarial values.

The subcompartmented oxphosomic model of the phosphorylating system organization in mitochondria

The oxidative phosphorylation (OXPHOS) system of mitochondria supports all the vitally important energyconsuming processes in eukaryotic cells, providing them with energy in the form of ATP. OXPHOS enzymes (complexes I–V) are located in the inner mitochondrial membrane, mainly in the cristae subcompartment. At present, there is a large body of data evidencing that the respiratory complexes I, III2 and IV under in vivo conditions can physically interact with each other in diverse stoichiometry, thereby forming supercomplexes. Despite active accumulation of knowledge about the structure of the main supercomplexes of the OXPHOS system, its physical and functional organization in vivo remains unclear. Contemporary models of the OXPHOS system’s organization in the inner membrane of mitochondria are contradictory and presume the existence of either highly organized respiratory strings, or, by contrast, a set of randomly dispersed respiratory supercomplexes and complexes. Furthermore, it is assumed that ATP-synthase (complex V) does not form associations with respiratory enzymes and operates autonomously. Our latest data obtained on mitochondria of etiolated shoots of pea evidence the possibility of physical association between the respiratory supercomplexes and dimeric ATP-synthase. These data have allowed us to reconsider the contemporary concept of the phosphorylation system organization and propose a new subcompartmented oxphosomic model. According to this model, a substantial number of the OXPHOS complexes form oxphosomes, which in a definite stoichiometry include complexes I–V and are located predominantly in the cristae subcompartment of mitochondria in the form of highly organized strings or patches. These suprastructures represent “mini-factories” for ATP production. It is assumed that such an organization (1) contributes to increasing the efficiency of the OXPHOS system operation, (2) involves new levels of activity regulation, and (3) may determine the inner membrane morphology to some extent. The review discusses the proposed model in detail. For a better understanding of the matter, the history of development of concepts concerning the OXPHOS organization with the emphasis on recent contemporary models is briefly considered. The principal experimental data accumulated over the past 40 years, which confirm the validity of the oxphosomic hypothesis, are also provided.

Integrated mRNA-miRNA transcriptome analysis of bladder biopsies from patients with bladder pain syndrome identifies signaling alterations contributing to the disease pathogenesis

Background Interstitial cystitis, or bladder pain syndrome (IC/BPS), is a chronic bladder disorder characterized by lower abdominal pain associated with the urinary bladder and accompanied by urinary frequency and urgency in the absence of identifiable causes. IC/PBS can be separated into the classic Hunner’s ulcerative type and the more prevalent non-ulcerative disease. Our aim was to unravel the biological processes and dysregulated cell signaling pathways leading to the bladder remodeling in non-ulcerative bladder pain syndrome (BPS) by studying the gene expression changes in the patients’ biopsies. Methods We performed paired microRNA (miRNA) and mRNA expression profiling in the bladder biopsies of BPS patients with non-Hunner interstitial cystitis phenotype, using comprehensive Next-generation sequencing (NGS) and studied the activated pathways and altered biological processes based on the global gene expression changes. Paired mRNA-miRNA transcriptome analysis delineated the regulatory role of the dysregulated miRNAs by identifying their targets in the disease-induced pathways. Results EIF2 Signaling and Regulation of eIF4 and p70S6K Signaling, activated in response to cellular stress, were among the most significantly regulated processes during BPS. Leukotriene Biosynthesis nociceptive pathway, important in inflammatory diseases and neuropathic pain, was also significantly activated. The biological processes identified using Gene Ontology over-representation analysis were clustered into six main functional groups: cell cycle regulation, chemotaxis of immune cells, muscle development, muscle contraction, remodeling of extracellular matrix and peripheral nervous system organization and development. Compared to the Hunner’s ulcerative type IC, activation of the immune pathways was modest in non-ulcerative BPS, limited to neutrophil chemotaxis and IFN-γ-mediated signaling. We identified 62 miRNAs, regulated and abundant in BPS and show that they target the mRNAs implicated in eIF2 signalling pathway. Conclusions The bladders of non-ulcerative BPS patients recruited in this study had alterations consistent with a strong cell proliferative response and an up-regulation of smooth muscle contractility, while the contribution of inflammatory processes was modest. Pathway analysis of the integrated mRNA-miRNA NGS dataset pinpointed important regulatory miRNAs whose dysregulation might contribute to the pathogenesis. Observed molecular changes in the peripheral nervous system organization and development indicate the potential role of local bladder innervation in the pain perceived in this type of BPS.

The subject-personal characteristics of military university officers as predictors of burnout

The results of the study of the subject-personal characteristics of military university officers (hereinafter officers) as predictors of burnout are represented. 73 officers (men) at the age of 23 to 51 years were examined. Negative relationships between phases of burnout and subjective (cognitive, volitional, emotional control, behavioral regulation, communicative potential) and personal (meaningfulness of life, moral normality) characteristics among officers have been revealed. Individual components of subject-personal characteristics are in close interrelation, which indicates that we are dealing with a complex system organization. There are significant differences among officers with various kinds of activity in terms of indicators of subject-personal characteristics. There are specific interrelations between subject-personal characteristics and phases of burnout of officers, depending on the type of official activity.

Pharmacists’ Perceived Barriers to Human Papillomavirus (HPV) Vaccination: A Systematic Literature Review

About 45:000 cancers are linked to HPV each year in the United States alone. The HPV vaccine prevents cancer and is highly effective, yet vaccination coverage remains low. Pharmacies can play a meaningful role in increasing HPV vaccination access due to their availability and convenience. However, little is known about pharmacists’ perceived barriers to HPV vaccination. The objective of this systematic review was to summarize existing literature on perceived barriers to administering HPV vaccination reported by pharmacists. Barriers identified from selected studies were synthesized and further grouped into patient, parental, (pharmacist’s) personal, and system/organization barrier groups. Six studies were included in this review. The cost of the HPV vaccine, insurance coverage and reimbursement were commonly reported perceived barriers. Adolescent HPV vaccination barriers related to parental concerns, beliefs, and inadequate knowledge about the HPV vaccine. Perceived (pharmacist’s) personal barriers were related to lack of information and knowledge about HPV vaccine and recommendations. At the system/organization level, barriers reported included lack of time/staff/space; difficulty in series completion; tracking and recall of patient; perceived competition with providers; and other responsibilities/vaccines taking precedence. Future strategies involving pharmacy settings in HPV-related cancer prevention efforts should consider research on multilevel pharmacy-driven interventions addressing barriers.

Deconstruction of cultural space: death of the text

Starting from the understanding of the “death” of the text as a process of its deconstruction, fragmentation, simplification, when the text becomes a signal instead of a message, we will also consider more modern transformations of its role and system organization, leading to symbolic “death” through complication and transition to hypertext. The reasons for converting the text of a message into a narrative and into a participant in intertextual relations. The final departure to the pragmatic aspect of information exchange in various fields (interpersonal communication, advertising, journalism, didactics). Without death, a ritualized act, with the dominance of network forms of communication, its inclusion in the space of network content is impossible. Now, after the publication of the text, it has by and large only two ways: either it is interpreted and retransmitted in such a way that inevitably leads to the inflation of the content, or it is archived, which is equivalent to a “funeral”. In any of the variants, this is the “death” of the original author’s text, the fate of which we are considering. Speaking of the “death of the text”, we mean the transformation of the very general concept (communicative intention) that defines the functionality of modern textual activity. The leading characteristics of relevant information as a unit of communication are its trigger (motivating) potential and the ability to act as network content (liquidity). The features that characterize the text in the traditional sense: coherence, integrity, i.e. the ability to demonstrate the qualities of the “system”, gradually lose their significance in social practice.

OPTIMIZING TELEMEDICINE FRAMEWORK USING FOG COMPUTING FOR SMART HEALTHCARE SYSTEMS

This research is done for optimizing telemedicine framework by using fogging or fog computing for smart healthcare systems. Fog computing is used to solve the issues that arise on telemedicine framework of smart healthcare system like Infrastructural, Implementation, Acceptance, Data Management, Security, Bottleneck system organization, and Network latency Issues. we mainly used Distributed Data Flow (DDF) method using fog computing in order to fully solve the listed issues.