Effects of infrared light laser therapy in vivo and in vitro periodontitis models

Light-controlled artificial molecular machines hold tremendous potential to revolutionize molecular sciences as autonomous motion allows the design of smart materials and systems whose properties can respond, adapt, and be modified on command. One long-standing challenge toward future applicability has been the need to develop methods using low-energy, low-intensity, near-infrared light to power these nanomachines. Here, we describe a rotary molecular motor sensitized by a two-photon absorber, which efficiently operates under near-infrared light at intensities and wavelengths compatible with in vivo studies. Time-resolved spectroscopy was used to gain insight into the mechanism of energy transfer to the motor following initial two-photon excitation. Our results offer prospects toward in vitro and in vivo applications of artificial molecular motors.

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Diagnostic Validity of Digital Imaging Fiber-Optic Transillumination (DIFOTI) and Near-Infrared Light Transillumination (NILT) for Caries in Dentine

Journal of Clinical Medicine ◽

10.3390/jcm9020420 ◽

2020 ◽

Vol 9 (2) ◽

pp. 420

Author(s):

Ana Marmaneu-Menero ◽

José Enrique Iranzo-Cortés ◽

Teresa Almerich-Torres ◽

José Carmelo Ortolá-Síscar ◽

José María Montiel-Company ◽

...

Keyword(s):

Confidence Interval ◽

Qualitative Analysis ◽

Near Infrared ◽

Meta Analysis ◽

Infrared Light ◽

X Rays ◽

Carious Lesions ◽

Sensitivity Specificity

The objective of the study is to analyse the available evidence for the validity of the transillumination method in the diagnosis of interproximal caries. Bibliographic searches were carried out in three data bases (PubMed, Embase, Scopus) with the key words “Transillumination AND caries”. A total of 11 studies were selected for the qualitative analysis and meta-analysis. In the qualitative analysis, both in vivo and in vitro studies were included. The gold standards were tomography, digital radiography, and clinical visual diagnosis. The meta-analysis determined the sensitivity, specificity, and area below the ROC curve relative to the transillumination method in the diagnosis of caries in dentine. Meta-analysis results obtained for transillumination gave a sensitivity value of 0.69 (confidence interval: 0.54–0.81), a specificity value of 0.89 (confidence interval: 0.61–0.98), while giving an AUC value of 0.79 (confidence interval: 0.67–0.87). Transillumination is a method offering moderate validity in the diagnosis of carious lesions in dentine, there is no strong evidence that may enable us to affirm that transillumination may fully substitute X-rays in the complementary diagnosis of carious lesions

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In Vivo Assessment of Thermosensitive Liposomes for the Treatment of Port Wine Stains by Antifibrinolytic Site-Specific Pharmaco-Laser Therapy

Pharmaceutics ◽

10.3390/pharmaceutics12060591 ◽

2020 ◽

Vol 12 (6) ◽

pp. 591

Author(s):

Mingjuan Li ◽

M. Ingmar van Raath ◽

Shervin Khakpour ◽

Ahmet Seçilir ◽

Bart C. Sliggers ◽

...

Keyword(s):

Laser Therapy ◽

Experimental Treatment ◽

Port Wine ◽

Site Specific ◽

Port Wine Stains ◽

Antifibrinolytic Drugs ◽

Thermosensitive Liposomes ◽

Local Release

Antifibrinolytic site-specific pharmaco-laser therapy (SSPLT) is an experimental treatment modality for refractory port wine stains (PWS). Conceptually, antifibrinolytic drugs encapsulated in thermosensitive liposomes are delivered to thrombi that form in semi-photocoagulated PWS blood vessels after conventional laser treatment. Local release of antifibrinolytics is induced by mild hyperthermia, resulting in hyperthrombosis and complete occlusion of the target blood vessel (clinical endpoint). In this study, 20 thermosensitive liposomal formulations containing tranexamic acid (TA) were assayed for physicochemical properties, TA:lipid ratio, encapsulation efficiency, and endovesicular TA concentration. Two candidate formulations (DPPC:DSPE-PEG, DPPC:MPPC:DSPE-PEG) were selected based on optimal properties and analyzed for heat-induced TA release at body temperature (T), phase transition temperature (Tm), and at T > Tm. The effect of plasma on liposomal stability at 37 °C was determined, and the association of liposomes with platelets was examined by flow cytometry. The accumulation of PEGylated phosphocholine liposomes in laser-induced thrombi was investigated in a hamster dorsal skinfold model and intravital fluorescence microscopy. Both formulations did not release TA at 37 °C. Near-complete TA release was achieved at Tm within 2.0–2.5 min of heating, which was accelerated at T > Tm. Plasma exerted a stabilizing effect on both formulations. Liposomes showed mild association with platelets. Despite positive in vitro results, fluorescently labeled liposomes did not sufficiently accumulate in laser-induced thrombi in hamsters to warrant their use in antifibrinolytic SSPLT, which can be solved by coupling thrombus-targeting ligands to the liposomes.

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H2O2/near-infrared light-responsive nanotheronostics for MRI-guided synergistic chemo/photothermal cancer therapy

Nanomedicine ◽

10.2217/nnm-2019-0043 ◽

2019 ◽

Vol 14 (16) ◽

pp. 2189-2207

Author(s):

Yiming Yu ◽

Li Zhang ◽

Miao Wang ◽

Zhe Yang ◽

Leping Lin ◽

...

Keyword(s):

Near Infrared ◽

Tumor Model ◽

Mri Contrast Agent ◽

Infrared Light ◽

Antitumor Effects ◽

Mri Contrast ◽

Nir Light ◽

Nir Laser

Aim: To develop a H2O2/near-infrared (NIR) laser light-responsive nanoplatform (manganese-doped Prussian blue@polypyrrole [MnPB@PPy]) for synergistic chemo/photothermal cancer theranostics. Materials & methods: Doxorubicin (DOX) was loaded onto the surface of polypyrrole shells. The in vitro and in vivo MRI performance and anticancer effects of these nanoparticles (NPs) were evaluated. Results: The MnPB@PPy NPs could not only generate heat under NIR laser irradiation for cancer photothermal therapy but also act as an excellent MRI contrast agent. The loaded DOX could be triggered to release by both NIR light and H2O2 to enhance synergistic therapeutic efficacy. The antitumor effects were confirmed by in vitro cellular cytotoxicity assays and in vivo treatment in a xenograft tumor model. Conclusion: The designed H2O2/NIR light-responsive MnPB@PPy-DOX NPs hold great potential for future biomedical applications.

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Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies

Aesthetic Plastic Surgery ◽

10.1007/s00266-015-0524-6 ◽

2015 ◽

Vol 39 (5) ◽

pp. 778-782 ◽

Cited By ~ 16

Author(s):

Kyung Hee Min ◽

Jin Hwan Byun ◽

Chan Yeong Heo ◽

Eun Hee Kim ◽

Hye Yeon Choi ◽

...

Keyword(s):

Stem Cells ◽

Laser Therapy ◽

Low Level Laser Therapy ◽

In Vivo Studies ◽

Adipose Derived Stem Cells ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2017.06.002 ◽

2017 ◽

Vol 173 ◽

pp. 258-265 ◽

Cited By ~ 17

Author(s):

K.R. Fernandes ◽

A.M.P. Magri ◽

H.W. Kido ◽

J.R. Parisi ◽

L. Assis ◽

...

Keyword(s):

Laser Therapy ◽

In Vivo Studies

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Microscale optoelectronic infrared-to-visible upconversion devices and their use as injectable light sources

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1802064115 ◽

2018 ◽

Vol 115 (26) ◽

pp. 6632-6637 ◽

Cited By ~ 31

Author(s):

He Ding ◽

Lihui Lu ◽

Zhao Shi ◽

Dan Wang ◽

Lizhu Li ◽

...

Keyword(s):

Near Infrared ◽

Biological Fluids ◽

Visible Spectral Range ◽

Infrared Light ◽

Light Sources ◽

Fully Integrated ◽

Broadband Absorption ◽

In Vivo Experiments

Optical upconversion that converts infrared light into visible light is of significant interest for broad applications in biomedicine, imaging, and displays. Conventional upconversion materials rely on nonlinear light-matter interactions, exhibit incidence-dependent efficiencies, and require high-power excitation. We report an infrared-to-visible upconversion strategy based on fully integrated microscale optoelectronic devices. These thin-film, ultraminiaturized devices realize near-infrared (∼810 nm) to visible [630 nm (red) or 590 nm (yellow)] upconversion that is linearly dependent on incoherent, low-power excitation, with a quantum yield of ∼1.5%. Additional features of this upconversion design include broadband absorption, wide-emission spectral tunability, and fast dynamics. Encapsulated, freestanding devices are transferred onto heterogeneous substrates and show desirable biocompatibilities within biological fluids and tissues. These microscale devices are implanted in behaving animals, with in vitro and in vivo experiments demonstrating their utility for optogenetic neuromodulation. This approach provides a versatile route to achieve upconversion throughout the entire visible spectral range at lower power and higher efficiency than has previously been possible.

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Experimental and Clinical Applications of Red and Near-Infrared Photobiomodulation on Endothelial Dysfunction: A Review

Biomedicines ◽

10.3390/biomedicines9030274 ◽

2021 ◽

Vol 9 (3) ◽

pp. 274 ◽

Cited By ~ 1

Author(s):

Esteban Colombo ◽

Antonio Signore ◽

Stefano Aicardi ◽

Angelina Zekiy ◽

Anatoliy Utyuzh ◽

...

Keyword(s):

Endothelial Dysfunction ◽

Near Infrared ◽

Disease Risk ◽

In Vivo Studies ◽

Infrared Light ◽

Animal Cells ◽

Atp Production ◽

Main Regulator

Background: Under physiological conditions, endothelial cells are the main regulator of arterial tone homeostasis and vascular growth, sensing and transducing signals between tissue and blood. Disease risk factors can lead to their unbalanced homeostasis, known as endothelial dysfunction. Red and near-infrared light can interact with animal cells and modulate their metabolism upon interaction with mitochondria’s cytochromes, which leads to increased oxygen consumption, ATP production and ROS, as well as to regulate NO release and intracellular Ca2+ concentration. This medical subject is known as photobiomodulation (PBM). We present a review of the literature on the in vitro and in vivo effects of PBM on endothelial dysfunction. Methods: A search strategy was developed consistent with the PRISMA statement. The PubMed, Scopus, Cochrane, and Scholar electronic databases were consulted to search for in vitro and in vivo studies. Results: Fifty out of >12,000 articles were selected. Conclusions: The PBM can modulate endothelial dysfunction, improving inflammation, angiogenesis, and vasodilatation. Among the studies, 808 nm and 18 J (0.2 W, 2.05 cm2) intracoronary irradiation can prevent restenosis as well as 645 nm and 20 J (0.25 W, 2 cm2) can stimulate angiogenesis. PBM can also support hypertension cure. However, more extensive randomised controlled trials are necessary.

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Gold-Based Nanoplataform for the Treatment of Anaplastic Thyroid Carcinoma: A Step Forward

Cancers ◽

10.3390/cancers13061242 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1242

Author(s):

Mariana Amaral ◽

Adília J. Charmier ◽

Ricardo A. Afonso ◽

José Catarino ◽

Pedro Faísca ◽

...

Keyword(s):

Thyroid Carcinoma ◽

Cell Viability ◽

Near Infrared ◽

Anaplastic Thyroid Carcinoma ◽

Infrared Light ◽

Surface Plasmon Resonances ◽

Plasmon Resonances ◽

Near Future

Anaplastic thyroid carcinoma (ATC) is a very rare subtype of thyroid carcinoma and one of the most lethal malignancies. Poor prognosis is mainly associated with its undifferentiated nature, inoperability, and failing to respond to the typically used therapies for thyroid cancer. Photothermal Therapy (PTT) entails using light to increase tissues’ temperature, leading to hyperthermia-mediated cell death. Tumours are more susceptible to heat as they are unable to dissipate it. By using functionalized gold nanoparticles (AuNPs) that transform light energy into heat, it is possible to target the heat to the tumour. This study aims to formulate ATC-targeted AuNPs able to convert near-infrared light into heat, for PTT of ATC. Different AuNPs were synthetized and coated. Size, morphology, and surface plasmon resonances band were determined. The optimized coated-AuNPs were then functionalized with ligands to assess ATC’s specificity. Safety, efficacy, and selectivity were assessed in vitro. The formulations were deemed safe when not irradiated (>70% cell viability) and selective for ATC. However, when irradiated, holo-transferrin-AuNPs were the most cytotoxic (22% of cell viability). The biodistribution and safety of this formulation was assessed in vivo. Overall, this novel formulation appears to be a highly promising approach to evaluate in a very near future.

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