The isolation and analysis of cell wall material from the alcohol-insoluble residue of cabbage (Brassica oleracea var.capitata)

1980 ◽  
Vol 31 (12) ◽  
pp. 1257-1267 ◽  
Author(s):  
Barry J. H. Stevens ◽  
Robert R. Selvendran
Keyword(s):  
Cell Wall ◽  
Brassica Oleracea ◽  
Wall Material ◽  
Insoluble Residue ◽  
Cell Wall Material

scholarly journals Microstructural and Texturizing Properties of Partially Pectin-Depleted Cell Wall Material: The Role of Botanical Origin and High-Pressure Homogenization

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2644
Author(s):  
Jelle Van Audenhove ◽  
Tom Bernaerts ◽  
Novita I. Putri ◽  
Erick O. Okello ◽  
Luisa Van Rooy ◽  
...  
Keyword(s):  
High Pressure ◽  
Cell Wall ◽  
Binding Capacity ◽  
Wall Material ◽  
Insoluble Residue ◽  
High Pressure Homogenization ◽  
Cell Wall Material ◽  
Botanical Origin ◽  
Water Binding ◽  
A Cell

In the current study, the texturizing properties of partially pectin-depleted cell wall material (CWM) of apple, carrot, onion and pumpkin, and the potential of functionalization by high-pressure homogenization (HPH) were addressed. This partially pectin-depleted CWM was obtained as the unextractable fraction after acid pectin extraction (AcUF) on the alcohol-insoluble residue. Chemical analysis was performed to gain insight into the polysaccharide composition of the AcUF. The microstructural and functional properties of the AcUF in suspension were studied before HPH and after HPH at 20 and 80 MPa. Before HPH, even after the pectin extraction, the particles showed a cell-like morphology and occurred separately in the apple, onion and pumpkin AcUF and in a clustered manner in the carrot AcUF. The extent of disruption by the HPH treatments at 20 and 80 MPa was dependent on the botanical origin. Only for the onion and pumpkin AcUF, the water binding capacity was increased by HPH. Before HPH, the texturizing potential of the AcUFs was greatly varying between the different matrices. Whereas HPH improved the texturizing potential of the pumpkin AcUF, no effect and even a decrease was observed for the onion AcUF and the apple and carrot AcUF, respectively.

Synchrotron infrared microspectroscopy reveals the response of Sphagnum cell wall material to its aqueous chemical environment

2018 ◽  
Vol 15 (8) ◽  
pp. 513
Author(s):  
Ewen Silvester ◽  
Annaleise R. Klein ◽  
Kerry L. Whitworth ◽  
Ljiljana Puskar ◽  
Mark J. Tobin
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Chemical Environment ◽  
Wall Material ◽  
Organic Soils ◽  
Cell Wall Material ◽  
Acid Base ◽  
Widespread Species ◽  
Flowing Liquid ◽  
Infrared Microspectroscopy

Environmental contextSphagnum moss is a widespread species in peatlands globally and responsible for a large fraction of carbon storage in these systems. We used synchrotron infrared microspectroscopy to characterise the acid-base properties of Sphagnum moss and the conditions under which calcium uptake can occur (essential for plant tissue integrity). The work allows a chemical model for Sphagnum distribution in the landscape to be proposed. AbstractSphagnum is one the major moss types responsible for the deposition of organic soils in peatland systems. The cell walls of this moss have a high proportion of carboxylated polysaccharides (polygalacturonic acids), which act as ion exchangers and are likely to be important for the structural integrity of the cell walls. We used synchrotron light source infrared microspectroscopy to characterise the acid-base and calcium complexation properties of the cell walls of Sphagnum cristatum stems, using freshly sectioned tissue confined in a flowing liquid cell with both normal water and D2O media. The Fourier transform infrared spectra of acid and base forms are consistent with those expected for protonated and deprotonated aliphatic carboxylic acids (such as uronic acids). Spectral deconvolution shows that the dominant aliphatic carboxylic groups in this material behave as a monoprotic acid (pKa=4.97–6.04). The cell wall material shows a high affinity for calcium, with a binding constant (K) in the range 103.9–104.7 (1:1 complex). The chemical complexation model developed here allows for the prediction of the chemical environment (e.g. pH, ionic content) under which Ca2+ uptake can occur, and provides an improved understanding for the observed distribution of Sphagnum in the landscape.

scholarly journals Influence of Lignin on Digestibility of Forage Cell Wall Material

1986 ◽  
Vol 62 (6) ◽  
pp. 1703-1712 ◽  
Author(s):  
H. G. Jung ◽  
K. P. Vogel
Keyword(s):  
Cell Wall ◽  
Wall Material ◽  
Cell Wall Material

The Effect of Siduron Upon Barley Root Metabolism

Weed Science ◽  
1968 ◽  
Vol 16 (3) ◽  
pp. 344-347 ◽  
Author(s):  
Walter E. Splittstoesser
Keyword(s):  
Cell Wall ◽  
Nucleic Acid ◽  
Hordeum Vulgare ◽  
Shoot Growth ◽  
Acid Metabolism ◽  
Wall Material ◽  
Nucleic Acid Metabolism ◽  
Cell Wall Material ◽  
Cell Wall Synthesis ◽  
Root Metabolism

Barley (Hordeum vulgareL. var. Trail) root growth was inhibited at lower concentrations of 1-(2-methylcyclohexyl)-3-phenylurea (siduron) than was shoot growth. The influence of siduron upon root metabolism was assessed with excised roots grown in 0 or 5 ppm siduron. More glucose-U-14C and leucine-U-14C were degraded to CO2and less were incorporated into cell wall material and protein by roots grown in siduron. However, roots grown in siduron incorporated more adenine-8-14C into nucleic acids and degraded less adenine to CO2than roots grown in water. It was suggested that siduron disrupted the normal nucleic acid metabolism of barley roots which was necessary for protein and cell wall synthesis.

The impact of chemical structure on polyphenol bioaccessibility, as a function of processing, cell wall material and pH: A model system

2021 ◽  
Vol 289 ◽  
pp. 110304 ◽  
Author(s):  
Eden Eran Nagar ◽  
Liora Berenshtein ◽  
Inbal Hanuka Katz ◽  
Uri Lesmes ◽  
Zoya Okun ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chemical Structure ◽  
Model System ◽  
Wall Material ◽  
Cell Wall Material ◽  
The Impact

Pectolytic enzyme production by Phoma betae

1972 ◽  
Vol 50 (8) ◽  
pp. 1705-1709 ◽  
Author(s):  
William M. Bugbee
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Culture Cell ◽  
Susceptible Variety ◽  
Wall Material ◽  
Cell Wall Material ◽  
Storage Roots ◽  
Phoma Betae

Phoma betae from decayed sugar beet storage root tissue grew most rapidly in culture at 15C but produced more polygalacturonase (PG) at 20C. When the fungus was supplied with six different nitrogen sources, it produced the most PG on (NH4)2SO4.Assays of dialyzed culture filtrates using sodium polypectate and pectin or cell wall material from storage roots as the carbon sources showed the production of exopolygalacturonase (exo-PG) and endopolygalacturonate trans-eliminase (endo-PGTE). No pectin methyl esterase was detected. Exo-PG and endo-PGTE also were present in decayed sugar beet tissue. Only endo-PGTE was detected within 3 mm of tissue surrounding the rotted area.In culture, cell wall material from the susceptible variety A58 induced more endo-PGTE formation than the resistant 2B. But 2B induced more exo-PG formation than A58. It is suggested that endo-PGTE plays a major role in cell wall degradation because pH 7.5 was optimum for tissue maceration and pH 8.5 for enzyme activity and the advancing margins of rotted tissue contained only endo-PGTE.

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