A comprehensive study of the aerial parts of Lonicera japonica Thunb. based on metabolite profiling coupled with PLS‐DA

2020 ◽  
Vol 31 (6) ◽  
pp. 786-800 ◽  
Author(s):  
Zhichen Cai ◽  
Haiying Liao ◽  
Chengcheng Wang ◽  
Jiali Chen ◽  
Mengxia Tan ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Lonicera Japonica ◽  
Aerial Parts ◽  
Comprehensive Study

scholarly journals Chemical Composition of the Essential Oils from the Flower, Leaf and Stem of Lonicera japonica

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Nenad Vukovic ◽  
Miroslava Kacaniova ◽  
Lukas Hleba ◽  
Slobodan Sukdolak
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Lonicera Japonica ◽  
Hexadecanoic Acid ◽  
Plant Parts ◽  
Aerial Parts ◽  
Monoterpene Hydrocarbons

The essential oils from different aerial parts of Lonicera japonica have been extracted by hydro-distillation and analyzed by gas chromatography and gas chromatography coupled with mass spectrometry. Quantitative and qualitative differences were found between the analyzed plant parts. A total of eighty-nine compounds were identified. The main constituents were ( Z, Z)-farnesole (16.2%) and linalool (11.0%) for the flowers fraction, hexadecanoic acid (16.0%) and linalool (8.7%) for the leaves fraction, and hexadecanoic acid (31.4%) for the stems. Monoterpene hydrocarbons were absent from all the oils, and oxygenated sesquiterpenes were not identified in the essential oil of the stem.

Triterpenoid saponins from the aerial parts of Lonicera japonica

1994 ◽  
Vol 35 (4) ◽  
pp. 1005-1008 ◽  
Author(s):  
Kun Ho Son ◽  
Keun Young Jung ◽  
Hyeun Wook Chang ◽  
Hyun Pyo Kim ◽  
Sam Sik Kang
Keyword(s):  
Lonicera Japonica ◽  
Triterpenoid Saponins ◽  
Aerial Parts

scholarly journals Metabolite Profiling of Different Tissues of Barleria dinteri through the GC-MS Analysis

2021 ◽  
Vol 33 (6) ◽  
pp. 1336-1340
Author(s):  
Sechene S. Gololo ◽  
Chepape J. Semenya ◽  
Mutendela T. Olivier ◽  
Lesibana J. Sethoga ◽  
Emelinah H. Mathe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plant Species ◽  
Metabolite Profiling ◽  
Traditional Healers ◽  
Pharmacological Properties ◽  
Plant Parts ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Different Tissues ◽  
Different Parts

Barleria dinteri is a medicinal plant with distribution in the Limpopo, Gauteng and Mpumalanga provinces of South Africa as well as in Botswana, Swaziland and Namibia with exclusive specific habitation on limestone-rich soil. The plant species is used by traditional healers for wound healing and treatment of some intestinal tumours, as well as to relieve joint pains and toothache. The present study was aimed at the metabolite profiling of the different tissues (branches, flowers, leaves, roots) of Barleria dinteri using GC-MS analysis. Different extracts of the plant parts samples were subjected to GC-MS analysis and detected compounds were compared for presence amongst the different tissues. The results of the study revealed that all different parts (branches, flowers, leaves and roots) of B. dinteri, possess compounds that are detectable through GC-MS analysis with most compounds detected in the aerial parts, particularly the flowers. The results of the current study could serve as a basis for the possible plant parts substitution of the roots of B. dinteri with the aerial parts and the exploration of the pharmacological properties of the flowers for sustainable uses of the plant species for medicinal purposes.

Skin-permeating components of Lonicera japonica flos: a comprehensive study from observations and model computations

2019 ◽  
Vol 43 (32) ◽  
pp. 12538-12547
Author(s):  
Keda Zhang ◽  
Wanyang Sun ◽  
Alfred Fahr ◽  
Xiaobin Zeng ◽  
Lanlan Ge ◽  
...  
Keyword(s):  
Essential Oils ◽  
Iridoid Glycosides ◽  
Lonicera Japonica ◽  
Chlorogenic Acids ◽  
Comprehensive Study

Flavonoids and essential oils are well absorbed via the skin, while chlorogenic acids, iridoid glycosides and triterpenoids are poorly delivered.

Progress In The Practical Application Of Automated Image Analysis To Tem Negatives

1977 ◽  
Vol 35 ◽  
pp. 214-217
Author(s):  
F. A. Heckman ◽  
E. Redman ◽  
J.E. Connolly
Keyword(s):  
Image Analysis ◽  
Image Quality ◽  
Exposure Time ◽  
Image Contrast ◽  
Automated Image Analysis ◽  
Practical Application ◽  
Factors Affecting ◽  
High Image Quality ◽  
Qualitative Evidence ◽  
Comprehensive Study

In our initial publication on this subject1) we reported results demonstrating that contrast is the most important factor in producing the high image quality required for reliable image analysis. We also listed the factors which enhance contrast in order of the experimentally determined magnitude of their effect. The two most powerful factors affecting image contrast attainable with sheet film are beam intensity and KV. At that time we had only qualitative evidence for the ranking of enhancing factors. Later we carried out the densitometric measurements which led to the results outlined below.Meaningful evaluations of the cause-effect relationships among the considerable number of variables in preparing EM negatives depend on doing things in a systematic way, varying only one parameter at a time. Unless otherwise noted, we adhered to the following procedure evolved during our comprehensive study:Philips EM-300; 30μ objective aperature; magnification 7000- 12000X, exposure time 1 second, anti-contamination device operating.

Hexachlorobenzene toxicity in the monkey ovary: III. Ultrastructure induced by high (10 mg/kg) dose exposure

1991 ◽  
Vol 49 ◽  
pp. 130-131
Author(s):  
A. Singh ◽  
A. Dykeman ◽  
J. Jarrelf ◽  
D. C. Villeneuve
Keyword(s):  
Present Report ◽  
Reproductive Toxicity ◽  
Control Group ◽  
Primate Model ◽  
Human Follicular Fluid ◽  
Thin Sections ◽  
Cynomolgus Monkeys ◽  
Cacodylate Buffer ◽  
Induction Cycle ◽  
Comprehensive Study

Hexachlorobenzene (HCB), a persistent and mobile organochlorine pesticide, occurs in environment. HCB has been shown to be present in human follicular fluid. An objective of the present report, which is part of a comprehensive study on reproductive toxicity of HCB, was to determine the cytologic effects of the compound on ovarian follicles in a primate model.Materials and Methods. Eight Cynomolgus monkeys were housed under controlled conditions at Animal facility of Health and Welfare, Ottawa. Animals were orally administered gelatin capsules containing HCB mixed with glucose in daily dosages of 0.0 or 10 mg/kg b.w. for 90 days; the former was the control group. On the menstrual period following completion of dosing, the monkeys underwent an induction cycle of superovulation. At necropsy, one-half of an ovary from each animal was diced into ca. 2- to 3-mm cubed specimens that were fixed by immersion in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3). Subsequent procedures followed to obtain thin sections that were examined in a Hitachi H-7000 electron microscope have been described earlier.

Comparison of Substructures Between Uniaxial and Biaxial Deformation in 1100-0 Aluminum

1981 ◽  
Vol 39 ◽  
pp. 52-53
Author(s):  
D. L. Rohr ◽  
S. S. Hecker
Keyword(s):  
Plastic Strain ◽  
Cell Walls ◽  
Room Temperature ◽  
Mechanical Response ◽  
Biaxial Tension ◽  
Initial Deformation ◽  
Uniaxial Deformation ◽  
Biaxial Deformation ◽  
Stress States ◽  
Comprehensive Study

As part of a comprehensive study of microstructural and mechanical response of metals to uniaxial and biaxial deformations, the development of substructure in 1100 A1 has been studied over a range of plastic strain for two stress states.Specimens of 1100 aluminum annealed at 350 C were tested in uniaxial (UT) and balanced biaxial tension (BBT) at room temperature to different strain levels. The biaxial specimens were produced by the in-plane punch stretching technique. Areas of known strain levels were prepared for TEM by lapping followed by jet electropolishing. All specimens were examined in a JEOL 200B run at 150 and 200 kV within 24 to 36 hours after testing.The development of the substructure with deformation is shown in Fig. 1 for both stress states. Initial deformation produces dislocation tangles, which form cell walls by 10% uniaxial deformation, and start to recover to form subgrains by 25%. The results of several hundred measurements of cell/subgrain sizes by a linear intercept technique are presented in Table I.

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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