Acid anilides: VII. Quantitative analysis of fatty acids by paper chromatography of their anilides

The influence of formaldehyde on rat brain lipid was investigated by comparing the analysis of lipids from fresh rat brain with the analysis from rat brains fixed for 110 days in a formaldehyde solution. A quantitative analysis of the lipids using column and paper chromatography is described. Sphingomyclin, cerebrosides and sulphatides were not attacked. Only a slight diminution of phosphatidylserine was indicated. Phosphoinositides remain fairly constant. Of lecithin about 25% was converted to lyso-lecithin. The phosphatidylethanolamine and lyso-phosphatidylethanolamine fractions suffered a marked diminution and the reaction with formaldehyde was noticeable in a change of chromatographic behaviour. The fraction containing cholesterol, cholesterol esters, glycerides, and fatty acids increased, the increase being correlated with the decrease of other fractions by the formation of lyso compounds. After storing tissues for months in formaldehyde solutions a reliable analysis of the lipid content is still possible.

Download Full-text

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽

10.3390/molecules26020479 ◽

2021 ◽

Vol 26 (2) ◽

pp. 479

Author(s):

Shihan Wang ◽

Yuanshuai Gan ◽

Hong Kan ◽

Xinxin Mao ◽

Yongsheng Wang

Keyword(s):

Fatty Acids ◽

Quantitative Analysis ◽

Unsaturated Fatty Acids ◽

Internal Standard ◽

Active Components ◽

Reliable Determination ◽

External Standard Method ◽

Single Marker ◽

Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

Download Full-text

QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9s2.13480 ◽

2016 ◽

pp. 183

Author(s):

Parameswari P ◽

Devika Rengaswamy

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Quantitative Analysis ◽

Secondary Metabolites ◽

Bioactive Compounds ◽

Leaf Extract ◽

Metabolic Reaction ◽

Quantitative Investigation ◽

Artemisia Nilagirica ◽

Quantitative Examination

ABSTRACT Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on different human diseases. Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments for quantitative investigation of phytochemicals according to standard strategies. Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows separately. Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward extraction and refinement of specific bioactive mixes for human nourishment. Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.

Download Full-text