scholarly journals Zearalenone induces apoptosis of rat Sertoli cells through Fas-Fas ligand and mitochondrial pathway

2019 ◽  
Vol 34 (4) ◽  
pp. 424-433 ◽  
Author(s):  
Guodong Cai ◽  
Mengxue Si ◽  
Xi Li ◽  
Hui Zou ◽  
Jianhong Gu ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Fas Ligand ◽  
Mitochondrial Pathway

Resveratrol as an Enhancer of Apoptosis in Cancer: A Mechanistic Review

2020 ◽  
Vol 20 ◽  
Author(s):  
Milad Ashrafizadeh ◽  
Shahram Taeb ◽  
Hamed Haghi-Aminjan ◽  
Shima Afrashi ◽  
Kave Moloudi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Fas Ligand ◽  
Inhibitory Effect ◽  
Mitochondrial Pathway ◽  
Multi Drug Resistance ◽  
Chemotherapy Drugs ◽  
Normal Tissues ◽  
Anti Cancer

: Resistance of cancer cells to therapy is a challenge for achieving an appropriate therapeutic outcome. Cancer (stem) cells possess several mechanisms for increasing their survival following exposure to toxic agents such as chemotherapy drugs, radiation as well as immunotherapy. Evidences show that apoptosis plays a key role in response of cancer (stem) cells and their multi drug resistance. Modulation of both intrinsic and extrinsic pathways of apoptosis can increase efficiency of tumor response and amplify the therapeutic effect of radiotherapy, chemotherapy, targeted therapy and also immunotherapy. To date, several agents as adjuvant have been proposed to overcome resistance of cancer cells to apoptosis. Natural products are interesting because of low toxicity on normal tissues. Resveratrol is a natural herbal agent that has shown interesting anti-cancer properties. It has been shown to kill cancer cells selectively, while protecting normal cells. Resveratrol can augment reduction/oxidation (redox) reactions, thus increases the production of ceramide and the expression of apoptosis receptors such as Fas ligand (FasL). Resveratrol also triggers some pathways which induce mitochondrial pathway of apoptosis. On the other hand, resveratrol has an inhibitory effect on anti-apoptotic mediators such as nuclear factor κ B (NFκB), cyclooxygenase-2 (COX-2), phosphatidylinositol 3–kinase (PI3K) and mTOR. In this review, we explain the modulatory effects of resveratrol on apoptosis, which can augment the therapeutic efficiency of anti-cancer drugs or radiotherapy.

Defects in the regulatory clearance mechanisms favor the breakdown of self-tolerance during spontaneous autoimmune orchitis

2009 ◽  
Vol 296 (3) ◽  
pp. R743-R762 ◽  
Author(s):  
R.-Marc Pelletier ◽  
Suk Ran Yoon ◽  
Casimir D. Akpovi ◽  
Emil Silvas ◽  
María Leiza Vitale
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Plasma Membranes ◽  
Fas Ligand ◽  
Inflammatory Responses ◽  
Apoptotic Cell ◽  
Self Tolerance ◽  
Tnf Α ◽  
Fas L ◽  
Autoimmune Orchitis

We identified aberrations leading to spontaneous autoimmune orchitis (AIO) in mink, a seasonal breeder and natural model for autoimmunity. This study provides evidence favoring the view that a malfunction of the clearance mechanisms for apoptotic cell debris arising from imbalances in phagocyte receptors or cytokines acting on Sertoli cells constitutes a major factor leading to breakdown of self-tolerance during spontaneous AIO. Serum anti-sperm antibody titers measured by ELISA reflected spermatogenic activity without causing immune inflammatory responses. Orchitic mink showed excess antibody production accompanied by spermatogenic arrest, testicular leukocyte infiltration, and infertility. AIO serum labeled the postacrosomal region, the mid and end piece of mink sperm, whereas normal mink serum did not. Normal serum labeled plasma membranes, whereas AIO serum reacted with germ cell nuclei. Western blot analyses revealed that AIO serum reacted specifically to a 23- and 50-kDa protein. The number of apostain-labeled apoptotic cells was significantly higher in orchitic compared with normal tubules. However, apoptosis levels measured by ELISA in seminiferous tubular fractions (STf) were not significantly different in normal and orchitic tubules. The levels of CD36, TNF-α, TNF-α RI, IL-6, and Fas but not Fas-ligand (L), and ATP-binding cassette transporter ABCA1 were changed in AIO STf. TNF-α and IL-6 serum levels were increased during AIO. Fas localized to germ cells, Sertoli cells, and the lamina propria of the tubules and Fas-L, to germ cells. Fas colocalized with Fas-L in residual bodies in normal testis and in giant cells and infiltrating leukocytes in orchitic tubules.

Crambene induces pancreatic acinar cell apoptosis via the activation of mitochondrial pathway

2006 ◽  
Vol 291 (1) ◽  
pp. G95-G101 ◽  
Author(s):  
Yang Cao ◽  
Sharmila Adhikari ◽  
Abel Damien Ang ◽  
Marie Véronique Clément ◽  
Matthew Wallig ◽  
...  
Keyword(s):  
Acinar Cell ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Fas Ligand ◽  
Annexin V ◽  
Acinar Cells ◽  
Mitochondrial Pathway ◽  
Pancreatic Acinar Cell ◽  
Pancreatic Acinar Cells ◽  
Pancreatic Acini

We investigated the apoptotic pathway activated by crambene (1-cyano-2-hydroxy-3-butene), a plant nitrile, on pancreatic acinar cells. As evidenced by annexin V-FITC staining, crambene treatment for 3 h induced the apoptosis but not necrosis of pancreatic acini. Caspase-3, -8, and -9 activities in acini treated with crambene were significantly higher than in untreated acini. Treatment with caspase-3, -8, and -9 inhibitors inhibited annexin V staining, as well as caspase-3 activity, pointing to an important role of these caspases in crambene-induced acinar cell apoptosis. The mitochondrial membrane potential was collapsed, and cytochrome c was released from the mitochondria in crambene-treated acini. Neither TNF-α nor Fas ligand levels were changed in pancreatic acinar cells after crambene treatment. These results provide evidence for the induction of pancreatic acinar cell apoptosis in vitro by crambene and suggest the involvement of mitochondrial pathway in pancreatic acinar cell apoptosis.

N-nitrosodimethylamine (NDMA) induced apoptosis dependent on Fas/FasL complex in human leukocytes

2019 ◽  
Vol 38 (5) ◽  
pp. 578-587
Author(s):  
A Iwaniuk ◽  
K Grubczak ◽  
W Ratajczak-Wrona ◽  
M Garley ◽  
K Nowak ◽  
...  
Keyword(s):  
Fas Ligand ◽  
Mononuclear Cells ◽  
Mitochondrial Pathway ◽  
Polymorphonuclear Neutrophils ◽  
Apoptotic Cells ◽  
Ligand Complex ◽  
Peripheral Blood Mononuclear ◽  
Human Leukocytes ◽  
Antiapoptotic Proteins ◽  
Induced Apoptosis

Objective: To investigate the mechanism of apoptosis dependent on the Fas/FasL (Fas ligand) complex in the presence of N-nitrosodimethylamine (NDMA) in human leukocytes. Methods: Polymorphonuclear neutrophils (PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated form whole blood by density centrifugation. The concentration of NDMA was assessed by cellular toxicity assay. Apoptotic cells were assessed with flow cytometry and the expression of pro- and antiapoptotic proteins was investigated by Western blotting in PMNs and PBMCs treated with NDMA and/or FasL. Results: PMNs showed a higher ratio of apoptotic cells than PBMCs after exposure to NDMA and/or FasL. Enhanced apoptosis was related to the increased expression of proapoptotic proteins in neutrophils following exposure to either NDMA or FasL. In PBMCs, the relation was observed after exposure to FasL only. PMNs and PBMCs incubated with NDMA and FasL simultaneously demonstrated the highest increase in protein expression. Conclusions: NDMA shows a stronger proapoptotic effect with PMNs than with PBMCs. The Fas/FasL complex, along with other proapoptotic proteins of the receptor (Fas, FADD) and mitochondrial pathway (Noxa, Puma, Bim), plays a key role in the induction of neutrophil apoptosis. Synergic effects of NDMA and FasL which lead to higher induction of apoptosis in PMNs than in PBMCs indicates a multistage and varied regulation of apoptosis in different populations of leukocytes.

scholarly journals Constitutive Fas Ligand Gene Transcription in Sertoli Cells Is Regulated By Sp1

1999 ◽  
Vol 274 (12) ◽  
pp. 7756-7762 ◽  
Author(s):  
Rebecca F. McClure ◽  
Carrie J. Heppelmann ◽  
Carlos V. Paya
Keyword(s):  
Gene Transcription ◽  
Sertoli Cells ◽  
Fas Ligand

p,p′-Dichlorodiphenoxydichloroethylene induced apoptosis of Sertoli cells through oxidative stress-mediated p38 MAPK and mitochondrial pathway

2011 ◽  
Vol 202 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Yang Song ◽  
Yuqin Shi ◽  
Haige Yu ◽  
Yafei Hu ◽  
Yinan Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
P38 Mapk ◽  
Sertoli Cells ◽  
Mitochondrial Pathway ◽  
Induced Apoptosis

scholarly journals The Immunoprotective Effect of Sertoli Cells Coencapsulated with Islet Xenografts is Not Dependent upon Fas Ligand Expression

2002 ◽  
Vol 11 (8) ◽  
pp. 799-801 ◽  
Author(s):  
Hua Yang ◽  
Ayman Al-Jazaeri ◽  
James R. Wright
Keyword(s):  
Graft Survival ◽  
Sertoli Cells ◽  
Fas Ligand ◽  
Testicular Cell ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Fas L ◽  
Cell Fractions

Coencapsulation with Sertoli-enriched testicular cell fractions prolongs islet graft survival time compared with islet encapsulation alone in a highly discordant tilapia (fish)-to-mouse xenotransplantation model. Here we investigate whether Fas ligand (Fas-L) expression by testicular Sertoli cells is responsible for this additional protective effect. Sertoli-enriched testicular cell fractions (7 × 106 cells) harvested from either Fas-L-defective (group I) or Fas-L-positive (group II) mice were coencapsulated in alginate gel spheres with fish islets and then transplanted into streptozotocin-diabetic Balb/c recipients. Group III mice received encapsulated islets without coencapsulated Sertoli cells. After transplantation, blood glucose levels were monitored three times per week. Mean graft survival times for the three groups were: group I = 35.6 ± 10.2 days (n = 9), group II = 31.3 ± 9.4 days (n = 7), and group III = 23.3 ± 2.2 days (n = 6) (ANOVA, p = 0.043). Coencapsulation, regardless of the Fas-L status of the Sertoli cell donors, modestly prolonged graft survival. There was no significant difference between Fas-L-deficient and Fas-L-positive donors. Our results suggest that Fas/Fas-L interaction is not responsible for the additional protection afforded to encapsulated discordant islet xenografts by coencapsulation with Sertoli cells.

Lipopolysaccharide-induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro-inflammatory cytokines andmiR-187

2015 ◽  
Vol 82 (11) ◽  
pp. 880-891 ◽  
Author(s):  
Yi Wang ◽  
Jiao-Jiao Zhang ◽  
Wei-Rong Yang ◽  
Hong-Yan Luo ◽  
Jia-Hua Zhang ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Sertoli Cells ◽  
Fas Ligand ◽  
Induced Expression ◽  
Pro Inflammatory Cytokines

Osthole induces human nasopharyngeal cancer cells apoptosis through Fas-Fas ligand and mitochondrial pathway

2018 ◽  
Vol 33 (4) ◽  
pp. 446-453 ◽  
Author(s):  
Pei-Ying Liu ◽  
Dun-Cheng Chang ◽  
Yu-Sheng Lo ◽  
Yi-Ting Hsi ◽  
Chia-Chieh Lin ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fas Ligand ◽  
Nasopharyngeal Cancer ◽  
Mitochondrial Pathway
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