Recombination: RNA — A Powerful Tool for Recombination and Regulated Expression of Genes

2005 ◽  
pp. 31-49 ◽  
Author(s):  
Dirk Müller ◽  
Ulf Stahl
Keyword(s):  
Expression Of Genes ◽  
Regulated Expression

scholarly journals Regulated expression of genes inserted at the human chromosomal beta-globin locus by homologous recombination.

1988 ◽  
Vol 85 (11) ◽  
pp. 3845-3849 ◽  
Author(s):  
A. K. Nandi ◽  
R. S. Roginski ◽  
R. G. Gregg ◽  
O. Smithies ◽  
A. I. Skoultchi
Keyword(s):  
Homologous Recombination ◽  
Beta Globin ◽  
Expression Of Genes ◽  
Regulated Expression

scholarly journals In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn7 Transposon with an Outward-Facing Inducible Promoter

2008 ◽  
Vol 74 (11) ◽  
pp. 3419-3425 ◽  
Author(s):  
Christophe Bordi ◽  
Bronwyn G. Butcher ◽  
Qiaojuan Shi ◽  
Anna-Barbara Hachmann ◽  
Joseph E. Peters ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Frequency ◽  
Inducible Promoter ◽  
In Vitro Mutagenesis ◽  
Donor Plasmid ◽  
Positive Bacterium ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Selection Of

ABSTRACT A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.

Anther-specific, developmentally regulated expression of genes encoding a new class of proline-rich proteins in sunflower

1991 ◽  
Vol 16 (2) ◽  
pp. 271-281 ◽  
Author(s):  
Jean-Luc Evrard ◽  
Colette Jako ◽  
Agn�s Saint-Guily ◽  
Jacques-Henry Weil ◽  
Marcel Kuntz
Keyword(s):  
Developmentally Regulated ◽  
New Class ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding

scholarly journals A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of Drosophila immune defenses

2021 ◽  
Vol 118 (12) ◽  
pp. e2100825118
Author(s):  
Di Chen ◽  
Arghyashree Roychowdhury-Sinha ◽  
Pragya Prakash ◽  
Xiao Lan ◽  
Wenmin Fan ◽  
...  
Keyword(s):  
Time Course ◽  
Adult Males ◽  
Transcriptomic Response ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding ◽  
Lag Period ◽  
Induced Genes ◽  
As Stress ◽  
Kinetics Of

Oncogenic RasV12 cells [A. Simcox et al., PLoS Genet. 4, e1000142 (2008)] injected into adult males proliferated massively after a lag period of several days, and led to the demise of the flies after 2 to 3 wk. The injection induced an early massive transcriptomic response that, unexpectedly, included more than 100 genes encoding chemoreceptors of various families. The kinetics of induction and the identities of the induced genes differed markedly from the responses generated by injections of microbes. Subsequently, hundreds of genes were up-regulated, attesting to intense catabolic activities in the flies, active tracheogenesis, and cuticulogenesis, as well as stress and inflammation-type responses. At 11 d after the injections, GFP-positive oncogenic cells isolated from the host flies exhibited a markedly different transcriptomic profile from that of the host and distinct from that at the time of their injection, including in particular up-regulated expression of genes typical for cells engaged in the classical antimicrobial response of Drosophila.

scholarly journals Mutation Types of CYP71P1 Cause Different Phenotypes of Mosaic Spot Lesion and Premature Leaf Senescence in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuhan Zheng ◽  
Jiangmin Xu ◽  
Fujun Wang ◽  
Yongchao Tang ◽  
Zheng Wei ◽  
...  
Keyword(s):  
Cell Death ◽  
Indica Rice ◽  
Photosynthetic Pigment ◽  
Single Amino Acid ◽  
Blue Staining ◽  
Premature Senescence ◽  
Lesion Mimic ◽  
Expression Of Genes ◽  
Single Amino Acid Change ◽  
Regulated Expression

Lesion mimic mutants (LMMs) are ideal materials for studying programmed cell death and defense response in plants. Here we report investigations on two LMMs (msl-1 and msl-2) from the indica rice cultivar JG30 treated by ethyl methyl sulfone. Both of the mutants showed similar mosaic spot lesions at seedling stage, but they displayed different phenotypes along with development of the plants. At tillering stage, larger orange spots appeared on leaves of msl-2, while only small reddish-brown spots exhibit on leaves of msl-1. At heading stage, the msl-2 plants were completely dead, while the msl-1 plants were still alive even if showed apparent premature senility. For both the mutants, the mosaic spot lesion formation was induced by light; DAB and trypan blue staining showed a large amount of hydrogen peroxide accumulated at the lesion sites, accompanied by a large number of cell death. Consequently, reactive oxygen species were enriched in leaves of the mutants; SOD and CAT activities in the scavenging enzyme system were decreased compared with the wild type. In addition, degraded chloroplasts, decreased photosynthetic pigment content, down-regulated expression of genes associated with chloroplast synthesis/photosynthesis and up-regulated expression of genes related to senescence were detected in the mutants, but the abnormality of msl-2 was more serious than that of msl-1 in general. Genetic analysis and map-based cloning revealed that the lesion mimic and premature senescence traits of both the mutants were controlled by recessive mutated alleles of the SL (Sekiguchi lesion) gene, which encodes the CYP71P1 protein belonging to cytochrome P450 monooxygenase family. The difference of mutation sites and mutation types (SNP-caused single amino acid change and SNP-caused early termination of translation) led to the different phenotypes in severity between msl-1 and msl-2. Taken together, this work revealed that the CYP71P1 is involved in regulation of both premature senescence and cell death in rice, and its different mutation sites and mutation types could cause different phenotypes in terms of severity.

Transforming growth factor-β-regulated expression of genes in macrophages implicated in the control of cholesterol homoeostasis

2006 ◽  
Vol 34 (6) ◽  
pp. 1141-1144 ◽  
Author(s):  
D.P. Ramji ◽  
N.N. Singh ◽  
P. Foka ◽  
S.A. Irvine ◽  
K. Arnaoutakis
Keyword(s):  
Growth Factor ◽  
Molecular Mechanisms ◽  
Transforming Growth Factor ◽  
Inflammatory Responses ◽  
Critical Role ◽  
Transforming Growth Factor Β ◽  
Crucial Importance ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Key Genes

The regulation of macrophage cholesterol homoeostasis is of crucial importance in the pathogenesis of atherosclerosis, an underlying cause of heart attack and stroke. Several recent studies have revealed a critical role for the cytokine TGF-β (transforming growth factor-β), a key regulator of the immune and inflammatory responses, in atherogenesis. We discuss here the TGF-β signalling pathway and its role in this disease along with the outcome of our recent studies on the action of the cytokine on the expression of key genes implicated in the uptake or efflux of cholesterol by macrophages and the molecular mechanisms underlying such regulation.

scholarly journals Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite

2019 ◽  
Vol 7 (8) ◽  
pp. 225 ◽  
Author(s):  
Shao ◽  
Tu ◽  
Wang ◽  
Jiang ◽  
Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Stress Response ◽  
Aspergillus Oryzae ◽  
Oxidative Stress Response ◽  
Sodium Bisulfite ◽  
Cellular Growth ◽  
Expression Of Genes ◽  
Menadione Sodium Bisulfite ◽  
Regulated Expression

Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous fungi, little information is available about Aspergillus oryzae. We investigated the effect of two oxidant agents (menadione sodium bisulfite, MSB, and hydrogen peroxide, H2O2) on cellular growth and antioxidant enzyme induction in A. oryzae. Results indicated severe inhibition of biomass and conidia production when high concentration of oxidants was used. Transcriptomic analysis showed an up-regulated expression of genes involved in oxidoreduction, such as catalase, glutathione peroxidase, and superoxide dismutase. In addition, it was observed that oxidative stress stimuli enhanced the expression of Yap1 and Skn7 transcription factors. Further, metabolomic analysis showed that glutathione content was increased in the oxidative treatments when compared with the control. Moreover, the content of unsaturated fatty acid decreased with oxidative treatment accompanying with the down-regulated expression of genes involved in linoleic acid biosynthesis. This study provided a global transcriptome characterization of oxidative stress response in A. oryzae, and can offer multiple target genes for oxidative tolerance improvement via genetic engineering.

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