Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin

The optimization and validation of a methodology for determining and extracting inorganic ionic Te(VI) and Te(IV) forms in easily-leached fractions of soil by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry (IC-ICP-MS) were studied. In this paper, the total concentration of Te, pH, and red-ox potential were determined. Ions were successfully separated in 4 min on a Hamilton PRPX100 column with 0.002 mg/kg and 0.004 mg/kg limits of detection for Te(VI) and Te(IV), respectively. Soil samples were collected from areas subjected to the influence of an electrowaste processing and sorting plant. Sequential chemical extraction of soils showed that tellurium was bound mainly with sulphides, organic matter, and silicates. Optimization of soil extraction allowed 20% average extraction efficiency to be obtained, using 100 mM citric acid as the extractant. In the tested soil samples, both tellurium species were present. In most cases, the soils contained a reduced Te form, or the concentrations of both species were similar.

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Nitrification, denitrification and anammox process coupled to iron redox in wetlands for domestic wastewater treatment

Journal of Cleaner Production ◽

10.1016/j.jclepro.2021.126953 ◽

2021 ◽

Vol 300 ◽

pp. 126953

Author(s):

Yuhui Ma ◽

Xiangyong Zheng ◽

Shengbing He ◽

Min Zhao

Keyword(s):

Wastewater Treatment ◽

Domestic Wastewater ◽

Domestic Wastewater Treatment ◽

Iron Redox ◽

Anammox Process

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Serum Concentration and Skin Expression of S100A7 (Psoriasin) in Patients Suffering from Hidradenitis Suppurativa

Dermatology ◽

10.1159/000510689 ◽

2020 ◽

pp. 1-7

Author(s):

Aleksandra Batycka-Baran ◽

Wojciech Baran ◽

Danuta Nowicka-Suszko ◽

Maria Koziol-Gałczyńska ◽

Andrzej Bieniek ◽

...

Keyword(s):

Serum Concentration ◽

Hidradenitis Suppurativa ◽

Protein Concentration ◽

Normal Skin ◽

Innate Immune ◽

Antimicrobial Protein ◽

Healthy Controls ◽

Serum Concentrations ◽

Reactive Protein

Background: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. An important role of innate immune dysregulation in the pathogenesis of HS has been highlighted. S100A7 (psoriasin) is an innate, antimicrobial protein that exerts proinflammatory and chemotactic action. Objectives: The objective of the study was to investigate serum concentrations of S100A7 in individuals with HS as compared to healthy controls. Further, we evaluated the expression of S100A7 in lesional HS skin as compared to perilesional (clinically uninvolved) HS skin and normal skin. Methods: Serum concentrations of S100A7 were evaluated with a commercially available ELISA kit. The expression of S100A7 in the skin was assessed using qRT-PCR and immunofluorescence staining. Results: We found increased expression of S100A7 in lesional HS skin as compared to perilesional HS skin (p = 0.0017). The expression of S100A7 in lesional HS skin was positively associated with serum C-reactive protein concentration and the severity of disease according to Hurley staging. The serum concentration of S100A7 in individuals with HS was decreased as compared to healthy controls and patients with psoriasis. Conclusions: Upregulated in lesional HS skin, S100A7 may enhance the inflammatory process and contribute to the HS pathogenesis.

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Toward a versatile flow technique: Development and application of reverse flow dual-injection analysis (rFDIA) for determining dissolved iron redox species and soluble reactive phosphorus in seawater

Talanta ◽

10.1016/j.talanta.2021.122404 ◽

2021 ◽

Vol 232 ◽

pp. 122404

Author(s):

Luodan Chen ◽

Jin Xu ◽

Ting Wang ◽

Yongming Huang ◽

Dongxing Yuan ◽

...

Keyword(s):

Reverse Flow ◽

Soluble Reactive Phosphorus ◽

Redox Species ◽

Dissolved Iron ◽

Dual Injection ◽

Reactive Phosphorus ◽

Iron Redox ◽

Technique Development

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Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes

Animals ◽

10.3390/ani11051203 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1203

Author(s):

Jerzy Juśkiewicz ◽

Aleksandra Rawicka ◽

Bartosz Fotschki ◽

Michał Majewski ◽

Zenon Zduńczyk

Keyword(s):

Fatty Acid ◽

Short Chain Fatty Acid ◽

Control Diet ◽

Chain Fatty Acid ◽

Short Chain ◽

Antimicrobial Protein ◽

Enzyme Release ◽

Bacterial Cells ◽

Suppression Effect ◽

Cecropin A

We hypothesised that the dietary addition of the bioactive antimicrobial protein lactoferrin (LF) and peptides melittin (MT) or cecropin A (CR) at a dosage of 100 mg/kg to the diet of Wistar rats would result in strong modulatory effects on faecal microbial enzymatic activity, short-chain fatty acid and ammonia concentrations. To date, the changes in bacterial extracellular and intracellular enzymatic activities upon addition of dietary AMPs have not yet been studied. This experiment lasted 15 days; during the first 5 day period, the rats were fed the control diet (S) and diets supplemented with LF, MT or CR. On days 6–15, all rats were fed the control S diet. The faecal fermentation processes were substantially stopped after two days of treatment, on average, in all rats receiving LF and two AMPs. The deepest suppression effect was observed on the last day of treatment (day 5) and persisted through days 5–8. The highest decreases in faecal bacterial β-glucosidase and β-glucuronidase activities as well as in SCFA and ammonia concentrations were observed in the rats fed the CR diet. Only in the CR animals did the mechanism of suppressed microbial fermentation involve diminished enzyme release from bacterial cells to the digesta.

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