Mass Spectrometry in Advancement of Redox Precision Medicine

2019 ◽  
pp. 327-358 ◽  
Author(s):  
Xiaofei Chen ◽  
Jingyun Lee ◽  
Hanzhi Wu ◽  
Allen W. Tsang ◽  
Cristina M. Furdui
Keyword(s):  
Mass Spectrometry ◽  
Precision Medicine

Proteomics and metabolomics applications in cancer biology

2019 ◽  
pp. 363-374
Author(s):  
Pedro Cutillas ◽  
Benedikt M. Kessler
Keyword(s):  
Mass Spectrometry ◽  
Molecular Biology ◽  
Precision Medicine ◽  
Clinical Outcomes ◽  
Cancer Progression ◽  
Cancer Biology ◽  
Cancer Metabolism ◽  
State Of The Art ◽  
Technical Challenges ◽  
Survival Mechanisms

Methodologies for proteomics and metabolomics are providing an unprecedented wealth of insights into cancer molecular biology. Although different techniques for proteomics and metabolomics exist, molecular snapshots in cancer metabolism and alterations in the proteome are mainly possible due to advancements in state-of-the-art mass spectrometry technologies. In this chapter, we describe examples of how proteo-metabolomic approaches are contributing to our understanding of the molecular biology of cancer progression, signalling, survival mechanisms, angiogenesis, and metastasis. We also provide an overview of the translational information (including biomarkers) and clinically relevant insights that proteomics and metabolomics strategies may be able to deliver, despite limitations and technical challenges that still exist. A better understanding of cancer progression and an improvement of clinical outcomes will benefit from precision medicine initiatives, in which appropriate application of proteo-metabolomic methods are key for their success.

Proteogenomics: From next-generation sequencing (NGS) and mass spectrometry-based proteomics to precision medicine

2019 ◽  
Vol 498 ◽  
pp. 38-46 ◽  
Author(s):  
Mia Yang Ang ◽  
Teck Yew Low ◽  
Pey Yee Lee ◽  
Wan Fahmi Wan Mohamad Nazarie ◽  
Victor Guryev ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Next Generation Sequencing ◽  
Precision Medicine ◽  
Next Generation ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

scholarly journals Proteogenomics and its applications in biology and precision medicine

2021 ◽  
Vol 19 (1) ◽  
pp. 1-14
Author(s):  
Phan Van Chi ◽  
Le Thi Bich Thao
Keyword(s):  
Mass Spectrometry ◽  
Precision Medicine ◽  
Open Reading Frames ◽  
Biological Processes ◽  
New Genes ◽  
Fundamental Research ◽  
Diagnostic Research ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Reading Frames

In this review, we briefly discuss proteogenomics, the integration of proteomics with genomics and transcriptomics, whereby the underlying technologies are next-generation sequencing (NGS) and mass spectrometry (MS) with processing the resulting data, an emerging field that promises to accelerate fundamental research related to transcription and translation, as well as its applicability. By combining genomic and proteomic information, scientists are achieving new results due to a more complete and unified understanding of complex molecular biological processes. Part of this review introduces some of the results of using proteogenomics in solving problems such as annotation, gene/genome re-annotation, including editing of open reading frames (ORFs), or improving a process to detect new genes in a number of different organisms, including humans. In particular, the paper also discusses the potential of proteogenomics through research achievements on human genome/proteome in precision medicine, especially in projects on phylogenetic and diagnostic research. and cancer treatment. The challenges and future of proteogenomics are also discussed and documented.

CHAPTER 10. Applications for Mass Spectrometry-based Proteomics and Phosphoproteomics in Precision Medicine

2020 ◽  
pp. 191-222
Author(s):  
Sara L. Banerjee ◽  
Ugo Dionne ◽  
Ana I. Osornio-Hernandez ◽  
Nicolas Bisson
Keyword(s):  
Mass Spectrometry ◽  
Precision Medicine

scholarly journals Mass Spectrometry in Precision Medicine: Phenotypic Measurements Alongside Pharmacogenomics

2016 ◽  
Vol 62 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Nigel J Clarke
Keyword(s):  
Mass Spectrometry ◽  
Precision Medicine ◽  
Drug Monitoring ◽  
Genetic Information ◽  
Patient Management ◽  
Enzyme Function ◽  
Medical Community ◽  
Therapeutic Drug ◽  
Phenotype Measurement ◽  
Genetic Makeup

Abstract BACKGROUND Precision medicine is becoming a major topic within the medical community and is gaining traction as a standard approach in many disciplines. This approach typically revolves around the use of a patient's genetic makeup to allow the physician to choose the appropriate course of treatment. In many cases the genetic information directs the drug to be used to treat the patient. In other cases the genetic markers associated with enzyme function may inform dosage recommendations. However there is a second way in which precision medicine can be practiced—that is, by therapeutic drug monitoring (TDM). CONTENT A review of the use of mass spectrometry for TDM in the arena of precision medicine is undertaken. Because the measurement of a drug or its metabolites provides the physician with a snapshot of the therapeutic exposure the patient is undergoing, these concentrations can be thought of as an actual phenotype measurement based around the patient's genetics coupled with all of the environmental, pharmacological, and nutritional variables. The outcome of a TDM measurement by mass spectrometry provides the patient's current phenotype vs the potential phenotype imputed by the genetics. SUMMARY The use of mass spectrometry can provide an understanding of how a drug is interacting with the patient, and is orthoganol to the information provided by pharmacogenomic assays. Further, the speed and relatively low expense of drug monitoring by mass spectrometry makes it an ideal test for precision medicine patient management.

scholarly journals Mass spectrometry-based chemical mapping and profiling toward molecular understanding of diseases in precision medicine

2021 ◽  
Author(s):  
Yechen Hu ◽  
Zhongcheng Wang ◽  
Liang Liu ◽  
Jianhua Zhu ◽  
Dongxue Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Precision Medicine ◽  
Chemical Mapping ◽  
Clinical Phenotypes

An overview of MS-based chemical mapping and profiling, indicating its contributions to the molecular understanding of diseases in precision medicine by answering "what", "where", "how many" and "whose” chemicals underlying clinical phenotypes.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Imaging of Al-Li-Cu alloys with a Scanning Ion Microprobe

1990 ◽  
Vol 48 (2) ◽  
pp. 314-315
Author(s):  
K.K. Soni ◽  
D.B. Williams ◽  
J.M. Chabala ◽  
R. Levi-Setti ◽  
D.E. Newbury
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Equilibrium Phase ◽  
Icosahedral Symmetry ◽  
Ion Microprobe ◽  
X Ray ◽  
Cu Alloys ◽  
Two Phases ◽  
The University ◽  
Secondary Ion

In contrast to the inability of x-ray microanalysis to detect Li, secondary ion mass spectrometry (SIMS) generates a very strong Li+ signal. The latter’s potential was recently exploited by Williams et al. in the study of binary Al-Li alloys. The present study of Al-Li-Cu was done using the high resolution scanning ion microprobe (SIM) at the University of Chicago (UC). The UC SIM employs a 40 keV, ∼70 nm diameter Ga+ probe extracted from a liquid Ga source, which is scanned over areas smaller than 160×160 μm2 using a 512×512 raster. During this experiment, the sample was held at 2 × 10-8 torr.In the Al-Li-Cu system, two phases of major importance are T1 and T2, with nominal compositions of Al2LiCu and Al6Li3Cu respectively. In commercial alloys, T1 develops a plate-like structure with a thickness <∼2 nm and is therefore inaccessible to conventional microanalytical techniques. T2 is the equilibrium phase with apparent icosahedral symmetry and its presence is undesirable in industrial alloys.

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