Good Microbiological Laboratory Practices

2020 ◽  
pp. 15-22
Author(s):  
Abhishek Chauhan ◽  
Tanu Jindal
Keyword(s):  
Microbiological Laboratory

Appointment of Antimicrobial Medications in a Hospital Depending on the Results of Microbiological Monitoring of HAI

2020 ◽  
Vol 65 (9-10) ◽  
pp. 21-27
Author(s):  
S. D. Mitrokhin ◽  
O. E. Orlova ◽  
I. V. Gosteva ◽  
A. S. Shkoda
Keyword(s):  
Therapy Monitoring ◽  
Hospital Environment ◽  
Antimicrobial Drugs ◽  
Microbiological Monitoring ◽  
Medical Institutions ◽  
Extreme Drug Resistance ◽  
Microbiological Laboratory ◽  
The Russian Federation ◽  
Medical Programs ◽  
Epidemiological Situation

One of the tasks of the hospital-s clinical pharmacologists service is to continuously monitor the consumption of antimicrobial drugs (DDD analysis) depending on the microbiological and epidemiological situation in the hospital. This is necessary for the implementation of various medical programs and technologies aimed at reducing the selection pressure of antimicrobial drugs and reducing the risk of the emergence, accumulation, and spread of bacteria strains with multiple and/or extreme drug resistance to these drugs in the hospital environment. To date, some medical institutions, especially in the various regions of the Russian Federation, do not have a proper team of clinical pharmacologists and a modern, well-equipped and computerized microbiological laboratory. This does not allow full implementation of the above-mentioned programs in such hospitals, e. g. Antimicrobial therapy monitoring system — SСAT and technologies, e. g. «carbapenem-preserving technologies» and «microbiological monitoring».

scholarly journals PHYSICOCHEMICAL AND MICROBIAL ANALYSIS OF FECES FROM HORSES FED DIETS CONTAINING CITRUS PULP

2016 ◽  
Vol 17 (4) ◽  
pp. 527-533
Author(s):  
Roberta Ariboni Brandi ◽  
Adriana Moraes de Oliveira Tribucci ◽  
Júlio César de Carvalho Balieiro ◽  
Ricardo Luiz Moro de Souza ◽  
Alfredo Manuel Franco Pereira ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Latin Square ◽  
Physicochemical Characteristics ◽  
Microbiological Analysis ◽  
Citrus Pulp ◽  
Plastic Bags ◽  
Microbiological Laboratory ◽  
Acid Producing Bacteria ◽  
Fecal Ph ◽  
Lactobacillus Spp

Abstract This study aimed to evaluate the effect of diets containing increasing levels of citrus pulp on the physicochemical and microbiological characteristics of horses feces. Five mares, at an average age of 3.5 years old and body weight of 492 ± 44.5 kg were arranged in a 5 x 5 Latin Square. The experimental diet consisted of 60% coast-cross hay and 40 % of concentrate with increasing levels of citrus pulp (0, 7, 14, 21, and 28 %). To determine the fecal pH, samples were collected directly from the floor, immediately after defecation, in the first feces of the day at 07:00 a.m., and color and fecal consistency were evaluated. For microbiological analysis, an aliquot was reserved in plastic bags, frozen, and sent to the microbiological laboratory for further analysis. Lactic acid bacteria were counted for Lactobacillus spp. and Streptococcus spp. from fecal samples under anaerobic conditions. The diet produced differences (P<0.05) in feces consistency: 98% of the animals had normal and firm stools, while 2% were loose ruminant-type feces. We observed no difference (P<0.05) for color, verifying 100% of the animals of greenish feces, normal for equines. There was no effect (P>0.05) on pH and on the number of Lactobacillus spp. and Streptococcus spp. The inclusion of up to 28% citrus pulp concentrates for horses did not promote change in the physicochemical characteristics and on the population of lactic acid-producing bacteria in feces.

scholarly journals ANALYSIS AND PREVENTION OF RISKS IN THE MANUFACTURE OF LABORATORY MICROBIOLOGICAL CULTURE MEDIA BY FMEA METHOD

2019 ◽  
Vol 14 (1) ◽  
pp. 90-98
Author(s):  
Vladimir E Nikitchenko ◽  
Ekaterina O Rystsova ◽  
Anastasiya N Chernysheva
Keyword(s):  
Risk Assessment ◽  
Culture Media ◽  
Negative Consequences ◽  
Nutrient Media ◽  
Effect Analysis ◽  
Risk Levels ◽  
Assessment Protocol ◽  
Operational Risks ◽  
Microbiological Laboratory ◽  
Fmea Method

At all stages of the production of microbiological nutrient media (MNM), the manufacturer, and in particular, the microbiological laboratories that manufacture the media on site, face many operational risks. The presence of such risks, in almost every critical point of production and further operation of the MNM, is due to the presence of common basic requirements for all MNM, which must be taken into account and respected during their development and preparation; as well as the complexity and laboriousness of the very process of manufacturing high-quality differential-diagnostic and other nutrient media, requiring the availability of all the components necessary for preparing these media, equipment, sterile conditions and qualified personnel. In this regard, there is a need to search for effective methods to identify and prevent undesirable situations associated with the production and use of MNM. The aim of this work was to adapt the risk assessment methodology based on the expert method for analyzing the types and consequences of FMEA failures (Failure Mode Effect Analysis) set out in GOST R ISO 31010-2011 for the needs of microbiological laboratories, including those for veterinary and sanitary expertise, producing microbiological nutrient environments and using them. As part of this work, a comparative analysis of risk assessment methods was carried out in order to select the optimal one; adaptation of the QMS principle - risk-oriented thinking and the FMEA method for risk assessment in the implementation of MNM manufacturing processes in a microbiological laboratory (for example, solid agar media); risk assessment protocol forms were developed; calculations of a quantitative assessment of risk levels were carried out in order to determine the need for preventive actions and their implementation in order to minimize the negative consequences of risk in case of its implementation using the developed protocols. The results showed that this technique can be successfully implemented and used in the claimed area.

scholarly journals Tuberculous Meningitis: The Microbiological Laboratory Diagnosis and Its Drug Sensitivity Patterns

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Titiek Sulistyowati ◽  
Deby Kusumaningrum ◽  
Eko Budi Koendhori ◽  
Ni Made Mertaniasih
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculous Meningitis ◽  
Drug Sensitivity ◽  
Mycobacterium Tuberculosis Complex ◽  
Laboratory Diagnosis ◽  
Clinical Microbiology Laboratory ◽  
Tuberculosis Complex ◽  
First Line ◽  
Sensitivity Pattern ◽  
Microbiological Laboratory

Background: Tuberculosis continues one of the major challenges to global health. Mycobacterium tuberculosis complex can affect any organ other than the lung parenchyma, include central nervous system. The mortality rate of tuberculous meningitis (TBM) are high worldwide with up to half of survivors suffering irreversible sequelae. Diagnosis of TBM is difficult due to paucibacillary, various clinical manifestation, and invasive procedure to appropriate specimens. Objective: The objectiveis to study the positivity rate of microbiological laboratory diagnosis and its drug sensitivity patterns of TBM patients in Dr. Soetomo Hospital Surabaya during October 2015 until September 2016. Methods: Specimens were cerebrospinal fluids. Identification and drug anti TB sensitivity test were done by BACTEC MGIT 960 system in Clinical Microbiology Laboratory Dr. Soetomo Hospital Surabaya. Result: Most patients with TBM were women (54.29%). Based on age groups, most dominant was adult population (65.71%). Proportion percentage of positive M. tuberculosis complex among 180 specimens were 19.44%. First line anti TB drug sensitivity pattern of 35 isolates were 1 monoresistant, 1 poly-resistant, no multiple drug resistant (MDR), and 33 pan-susceptible. Conclusion: Positivity rate of Mycobacterium tuberculosis complex laboratory diagnosis from TBM suspect patients were low. There was no MDR TB in this study, but mono-resistant and poly-resistant. Microbiological diagnosis was important to give information of active disease and drug sensitivity pattern. Resistance to first line anti TB drugs is alarming to properly manage TBM patients.

Determinants of Implementation of Isolation Precautions Against Infections by Multidrug-Resistant Microorganisms: A Hospital-Based, Multicenter, Observational Study

2017 ◽  
Vol 38 (10) ◽  
pp. 1188-1195
Author(s):  
Thomas Bénet ◽  
Raphaele Girard ◽  
Solweig Gerbier-Colomban ◽  
Cédric Dananché ◽  
Elisabeth Hodille ◽  
...  
Keyword(s):  
Clinical Sample ◽  
Generation Cephalosporin ◽  
Multidrug Resistant ◽  
University Hospitals ◽  
Medical Prescription ◽  
Multicenter Observational Study ◽  
Factors Associated ◽  
Healthcare Settings ◽  
Microbiological Laboratory ◽  
Main Factor

OBJECTIVESWe aimed to ascertain the factors associated with lack of isolation precautions (IP) in patients infected or colonized by third-generation cephalosporin-resistant Enterobacteriaceae (3GCR-E) and methicillin-resistant Staphylococcus aureus (MRSA) in hospital settings.DESIGNProspective surveillance and audit of practices.SETTINGThe study included 4 university hospitals in Lyon, France.PARTICIPANTSAll patients hospitalized between April and June in 2013 and 2015 were included. Case patients had ≥1 clinical sample positive for MRSA and/or 3GCR-E.METHODSFactors associated with the lack of IP implementation were identified using multivariate logistic regression. The incidence of MDRO infections was expressed per 10,000 patient days.RESULTSOverall, 57,222 patients accounting for 192,234 patient days of hospitalization were included, and 635 (1.1%) MDRO cases were identified. MRSA incidence was 2.5 per 10,000 patient days (95% confidence interval [95% CI], 2.1–3.0) and 3GCR-E incidence was 10.1 per 10,000 patient days (95% CI, 9.2–11.0), with no crude difference between 2013 and 2015 (P=.15 and P=.11, respectively). Among 3GCR-E, the main species were Escherichia coli (43.8%) and Klebsiella pneumoniae (31.0%). Isolation precautions were implemented in 78.5% of cases. Lack of IP implementation was independently associated with patient age, year, specialty, hospital, colonization compared with infection, and lack of medical prescription for IPs (adjusted odds ratio, 17.4; 95% CI, 8.5–35.8; P<.001).CONCLUSIONSMRSA and 3GCR-E infections and/or colonizations are frequent in healthcare settings, and IPs are implemented in most cases. When IPs are lacking, the main factor is the absence of medical prescription for IPs, underscoring the need for alerts to physicians by the microbiological laboratory and/or the infection control team.Infect Control Hosp Epidemiol 2017;38:1188–1195

scholarly journals Circulation of Vancomycin-Resistant Enterococci in Health Facilities of the Karachay-Cherkess Republic

2020 ◽  
pp. 51-55
Author(s):  
Kh.Kh. Batchaev ◽  
T.D. Pilipenko ◽  
L.G. Sereda ◽  
T.A. Petryuk
Keyword(s):  
Health Facilities ◽  
The Body ◽  
Cervical Canal ◽  
Vancomycin Resistant Enterococci ◽  
Maternity Hospitals ◽  
Microbiological Laboratory ◽  
Causative Agents ◽  
Health Care Associated Infections ◽  
The Russian Federation ◽  
Vancomycin Resistant

Introduction. Gram-positive enterococci are an integral part of the normal intestinal microflora. However, if they get into wounds or sterile cavities of the body, they may induce various diseases including wound infections, urethritis, peritonitis, sepsis, pyelonephritis, or damage other organs. These microorganisms also induce nosocomial infections and are among the causative agents of health care-associated infections (HAIs); thus, it is critical to control the spread of enterococci. Our purpose was to monitor circulation of vancomycin-resistant enterococci (VRE) in health facilities of the Karachay-Cherkess Republic (KCR) in 2016-2018. Materials and methods. We studied VRE strains isolated in the Microbiological Laboratory of the Center for Hygiene and Epidemiology in the Karachay-Cherkess Republic. During three years, the total of 281 strains of vancomycin-resistant enterococci were isolated from various biological materials received from obstetric, surgical, pediatric, and therapeutic hospitals and from outpatients undergoing treatment in polyclinics of the Karachay-Cherkess Republic. Results. According to our data, VRE rates per 100 examined patients were 2.34 in 2016, 3.4 in 2017, and 3.03 in 2018, and were comparable to the rates registered in the Russian Federation in the range of 3% to 22.7%. Discussion. When analyzing circulation of vancomycin-resistant enterococci, we established that the highest VRE rate (4.24 per 100 examined patients) was observed in maternity hospitals, while the lowest rate of 0.67% was observed in therapeutic departments. When studying sensitivity of 281 isolated VRE strains to eight antibacterial drugs, no strain resistant to all antibiotics was revealed. Sensitivity to linezolid, ampicillin, and penicillin was observed in 92.6%, 79.0%, and 66.2% of strains. Sensitivity to ciprofloxacin, gentamicin, tetracycline, and nitrofurantoin was 50% or less. VRE were mainly isolated from urine and discharge from the cervical canal and pharynx.

Microchip system for monitoring microbial physiological behaviour under drug influences

2009 ◽  
Vol 223 (6) ◽  
pp. 777-786 ◽  
Author(s):  
S Arora ◽  
C S Lim ◽  
J Y Foo ◽  
M K Sakharkar ◽  
P Dixit ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Single Step ◽  
Adaptive Mutation ◽  
Cell Behaviour ◽  
Effective Screening ◽  
E Coli ◽  
Similar Work ◽  
Drug Concentrations ◽  
Microbiological Laboratory

Single-step real-time high-throughput monitoring of drug influences on bacterial cell behaviour has become important with growing interests in personalized therapy and medication. Conventional microchip assemblies to perform similar work do exist. However, most of these devices have complex set-ups incorporating micromixers, separators, pumps, or valves. These microcomponents can sometimes damage the entities being monitored because of the creation of unfavourable biological environments. This paper presents a microchip-based system that enables single-step mixing of two solutions in various ratios, without the need for additional microcomponents such as mixers and pumps, in order to screen effectively their combinatory effects on cell outcomes. In this work, in-vitro experiments were carried out using ampicillin at various concentrations to investigate their effects on Escherichia coli ( E. coli). Results showed that the microchip provided effective screening, which yielded useful results such as effective dosages, ineffective dosages, and other possible outcomes; for instance, in this case, the occurrence of adaptive mutation of the bacteria at certain drug concentrations. Comparative microbiological laboratory tests were carried out as standard for confirmation of the results.

scholarly journals Importance of Selective Media for Recovery of Yeasts from Clinical Specimens

1999 ◽  
Vol 37 (11) ◽  
pp. 3731-3732 ◽  
Author(s):  
Per Sandven ◽  
Jørgen Lassen
Keyword(s):  
Clinical Studies ◽  
Mixed Cultures ◽  
Selective Media ◽  
Clinical Specimens ◽  
Sabouraud Agar ◽  
Microbiological Laboratory ◽  
Clinically Significant ◽  
Yeast Isolation

We compared the recovery of yeasts from clinical specimens cultured on routine bacteriological media to the recovery of yeast from specimens cultured on a selective fungal medium (Sabouraud agar). The use of Sabouraud agar was especially important in cases of mixed cultures, since in such cases yeast was recovered on bacteriological media from only 50% of 44 yeast-positive pus specimens and from 22.5% of 22 yeast-positive throat specimens. The use of a selective fungal medium is therefore necessary to ensure the detection of yeast in specimens containing a mixture of bacteria and yeasts. As a result, clinicians must request yeast isolation when clinically indicated, and the microbiological laboratory must add a selective fungal medium when clinically significant yeasts are likely to be encountered. It is also important that selective fungal media be used in clinical studies of yeast infections.

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