Carbonic anhydrases in calcified tissues

2000 ◽  
pp. 491-497 ◽  
Author(s):  
Teuvo A. Hentunen ◽  
Pirkko L. Härkönen ◽  
H. Kalervo Väänänen
Keyword(s):  
Carbonic Anhydrases ◽  
Calcified Tissues

Melatonin modifies tumor hypoxia and metabolism by inhibiting HIF-1α and energy metabolic pathway in the in vitro and in vivo models of breast cancer

2019 ◽  
Vol 2 (4) ◽  
pp. 83-98 ◽  
Author(s):  
André De Lima Mota ◽  
Bruna Vitorasso Jardim-Perassi ◽  
Tialfi Bergamin De Castro ◽  
Jucimara Colombo ◽  
Nathália Martins Sonehara ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Glucose Metabolism ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Carbonic Anhydrases ◽  
In Vivo Models ◽  
Ca Ix ◽  
Gene And Protein Expression

Breast cancer is the most common cancer among women and has a high mortality rate. Adverse conditions in the tumor microenvironment, such as hypoxia and acidosis, may exert selective pressure on the tumor, selecting subpopulations of tumor cells with advantages for survival in this environment. In this context, therapeutic agents that can modify these conditions, and consequently the intratumoral heterogeneity need to be explored. Melatonin, in addition to its physiological effects, exhibits important anti-tumor actions which may associate with modification of hypoxia and Warburg effect. In this study, we have evaluated the action of melatonin on tumor growth and tumor metabolism by different markers of hypoxia and glucose metabolism (HIF-1α, glucose transporters GLUT1 and GLUT3 and carbonic anhydrases CA-IX and CA-XII) in triple negative breast cancer model. In an in vitro study, gene and protein expressions of these markers were evaluated by quantitative real-time PCR and immunocytochemistry, respectively. The effects of melatonin were also tested in a MDA-MB-231 xenograft animal model. Results showed that melatonin treatment reduced the viability of MDA-MB-231 cells and tumor growth in Balb/c nude mice (p <0.05). The treatment significantly decreased HIF-1α gene and protein expression concomitantly with the expression of GLUT1, GLUT3, CA-IX and CA-XII (p <0.05). These results strongly suggest that melatonin down-regulates HIF-1α expression and regulates glucose metabolism in breast tumor cells, therefore, controlling hypoxia and tumor progression. 

Automated Characterization of Atheromatous Plaque in Intravascular Ultrasound Images Using Neuro Fuzzy Classifier

2012 ◽  
Vol 58 (4) ◽  
pp. 425-431 ◽  
Author(s):  
D. Selvathi ◽  
N. Emimal ◽  
Henry Selvaraj
Keyword(s):  
Intravascular Ultrasound ◽  
Human Error ◽  
Human Life ◽  
Classification Performance ◽  
Atheromatous Plaque ◽  
Ultrasound Images ◽  
Fuzzy Classifier ◽  
Neuro Fuzzy ◽  
Calcified Tissues

Abstract The medical imaging field has grown significantly in recent years and demands high accuracy since it deals with human life. The idea is to reduce human error as much as possible by assisting physicians and radiologists with some automatic techniques. The use of artificial intelligent techniques has shown great potential in this field. Hence, in this paper the neuro fuzzy classifier is applied for the automated characterization of atheromatous plaque to identify the fibrotic, lipidic and calcified tissues in Intravascular Ultrasound images (IVUS) which is designed using sixteen inputs, corresponds to sixteen pixels of instantaneous scanning matrix, one output that tells whether the pixel under consideration is Fibrotic, Lipidic, Calcified or Normal pixel. The classification performance was evaluated in terms of sensitivity, specificity and accuracy and the results confirmed that the proposed system has potential in detecting the respective plaque with the average accuracy of 98.9%.

Are Carbonic Anhydrases Suitable Targets to Fight Protozoan Parasitic Diseases?

2019 ◽  
Vol 25 (39) ◽  
pp. 5266-5278 ◽  
Author(s):  
Katia D'Ambrosio ◽  
Claudiu T. Supuran ◽  
Giuseppina De Simone
Keyword(s):  
Drug Resistance ◽  
Animal Models ◽  
Carbonic Anhydrase ◽  
Drug Target ◽  
Treatment Options ◽  
Parasitic Diseases ◽  
Carbonic Anhydrases ◽  
Extensive Drug Resistance ◽  
Protozoan Infections

Protozoans belonging to Plasmodium, Leishmania and Trypanosoma genera provoke widespread parasitic diseases with few treatment options and many of the clinically used drugs experiencing an extensive drug resistance phenomenon. In the last several years, the metalloenzyme Carbonic Anhydrase (CA, EC 4.2.1.1) was cloned and characterized in the genome of these protozoa, with the aim to search for a new drug target for fighting malaria, leishmaniasis and Chagas disease. P. falciparum encodes for a CA (PfCA) belonging to a novel genetic family, the η-CA class, L. donovani chagasi for a β-CA (LdcCA), whereas T. cruzi genome contains an α-CA (TcCA). These three enzymes were characterized in detail and a number of in vitro potent and selective inhibitors belonging to the sulfonamide, thiol, dithiocarbamate and hydroxamate classes were discovered. Some of these inhibitors were also effective in cell cultures and animal models of protozoan infections, making them of considerable interest for the development of new antiprotozoan drugs with a novel mechanism of action.

State of the Art on Carbonic Anhydrase Modulators for Biomedical Purposes

2019 ◽  
Vol 26 (15) ◽  
pp. 2558-2573 ◽  
Author(s):  
Murat Bozdag ◽  
Abdulmalik Saleh Alfawaz Altamimi ◽  
Daniela Vullo ◽  
Claudiu T. Supuran ◽  
Fabrizio Carta
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
State Of The Art ◽  
Cellular Level ◽  
Hydration Reaction ◽  
Carbonic Anhydrases ◽  
Crucial Importance ◽  
Learning Impairments ◽  
Current Review ◽  
Potential Use

The current review is intended to highlight recent advances in the search of new and effective modulators of the metalloenzymes Carbonic Anhydrases (CAs, EC 4.2.1.1) expressed in humans (h). CAs reversibly catalyze the CO2 hydration reaction, which is of crucial importance in the regulation of a plethora of fundamental processes at cellular level as well as in complex organisms. The first section of this review will be dedicated to compounds acting as activators of the hCAs (CAAs) and their promising effects on central nervous system affecting pathologies mainly characterized from memory and learning impairments. The second part will focus on the emerging chemical classes acting as hCA inhibitors (CAIs) and their potential use for the treatment of diseases.

scholarly journals Effect of Sulfonamides and Their Structurally Related Derivatives on the Activity of ι-Carbonic Anhydrase from Burkholderia territorii

2021 ◽  
Vol 22 (2) ◽  
pp. 571
Author(s):  
Viviana De Luca ◽  
Andrea Petreni ◽  
Alessio Nocentini ◽  
Andrea Scaloni ◽  
Claudiu T. Supuran ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Selective Inhibition ◽  
Extensive Study ◽  
Protein Isoforms ◽  
Carbonic Anhydrases ◽  
Physiological Processes ◽  
Microbial Survival ◽  
Reversible Hydration

Carbonic anhydrases (CAs) are essential metalloenzymes in nature, catalyzing the carbon dioxide reversible hydration into bicarbonate and proton. In humans, breathing and many other critical physiological processes depend on this enzymatic activity. The CA superfamily function and inhibition in pathogenic bacteria has recently been the object of significant advances, being demonstrated to affect microbial survival/virulence. Targeting bacterial CAs may thus be a valid alternative to expand the pharmacological arsenal against the emergence of widespread antibiotic resistance. Here, we report an extensive study on the inhibition profile of the recently discovered ι-CA class present in some bacteria, including Burkholderia territorii, namely BteCAι, using substituted benzene-sulfonamides and clinically licensed sulfonamide-, sulfamate- and sulfamide-type drugs. The BteCAι inhibition profile showed: (i) several benzene-sulfonamides with an inhibition constant lower than 100 nM; (ii) a different behavior with respect to other α, β and γ-CAs; (iii) clinically used drugs having a micromolar affinity. This prototype study contributes to the initial recognition of compounds which efficiently and selectively inhibit a bacterial member of the ι-CA class, for which such a selective inhibition with respect to other protein isoforms present in the host is highly desired and may contribute to the development of novel antimicrobials.

scholarly journals Effect of Crystal Growth on the Comparative Fixation of Sr89 and Ca45 by Calcified Tissues

1961 ◽  
Vol 236 (10) ◽  
pp. 2804-2806
Author(s):  
R.C. Likins ◽  
A.S. Posner ◽  
Boris Paretzkin ◽  
Ann P. Frost
Keyword(s):  
Crystal Growth ◽  
Calcified Tissues

scholarly journals Human Carbonic Anhydrases

1970 ◽  
Vol 245 (11) ◽  
pp. 2852-2856 ◽  
Author(s):  
S. Funakoshi ◽  
H.F. Deutsch
Keyword(s):  
Carbonic Anhydrases
Sign in / Sign up

Export Citation Format

Share Document