Synchrotron X-Ray Phase Nanotomography for Bone Tissue Characterization

2016 ◽  
pp. 1-42 ◽  
Author(s):  
Peter Varga ◽  
Loriane Weber ◽  
Bernhard Hesse ◽  
Max Langer
Keyword(s):  
Bone Tissue ◽  
Tissue Characterization ◽  
X Ray

Recent advances of the simulation of X-ray radiation propagation through bone tissue

2006 ◽  
Vol 39 ◽  
pp. S466
Author(s):  
M. Binkowski ◽  
Z. Król ◽  
Z. Wróbel ◽  
H.-F. Zeilhofer
Keyword(s):  
Bone Tissue ◽  
X Ray ◽  
Radiation Propagation ◽  
Recent Advances

scholarly journals Histological and X-ray structural analysis of the conditionof the bone tissue of the jaws with direct and delayed implantation using implants with different surface microstructures

2021 ◽  
Vol 108 (4) ◽  
pp. 24-32
Author(s):  
P. Grishin ◽  
◽  
E. Mamaeva ◽  
E. Kalinnikova ◽  
E. Kushner ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Key Words ◽  
Bone Tissue ◽  
Animal Experiment ◽  
Histological Analysis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Delayed Implantation ◽  
Surface Microstructures ◽  
Bone Condition

Abstract. This article presents the results of histological and X-ray studies of bone condition, stability, and degree of osteointegration of implants with different microstructure of the surface during immediate and delayed implantation in the animal experiment. The results of the study did not reveal significant differences in the process of osteointegration during direct and delayed implantation. Data from histological and X-ray studies of direct implantation in fresh extraction holes are comparable to those obtained during delayed implantation and are not inferior to the traditional two-stage protocol. At the same time, a certain correlation between the type of surface of the implant and the time of its adaptation in different periods of the experiment was revealed. When using an implant with an innovative surface, HSTTM. The process of osteointegration is mor clear, and the bone wall of the hole is more compacted, which indicates an accelerated and successful process of osteointegration. Key words: X-ray diffraction, frequency resonance and histological analysis, fibrous capsule, collagen fibers, trabeculae, bone tissue, periotestometry osseointegration, stability.

scholarly journals Dynamics of the mandible bone tissue condition after the dental implantation and shockwave therapy

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 175-179
Author(s):  
I.V. Chelpanova ◽  
O.Z. Masna-Chala ◽  
A.M. Yashchenko ◽  
Z.Z. Masna ◽  
Kh.I. Rudnytska
Keyword(s):  
Shock Wave ◽  
Bone Density ◽  
Bone Tissue ◽  
Bone Structure ◽  
Traumatic Injury ◽  
The Body ◽  
Maximum Pressure ◽  
Shock Wave Therapy ◽  
X Ray ◽  
Dental Implantation

Background. The problem of restoration the integrity of the dentition after tooth loss remains one of the most problematic dental issues. Мodern dental technologies open a promising direction in its solution, in particular - the method of dental implantation. Objective. The aim of our work was to study the changes that occur in the bone tissue of the mandible after implantation of titanium foam and to determine the effect of shock wave therapy (SWT) on its structure restoration. Methods. The study was performed on 15 adult rabbits aged 6-7 months, weighing 2.5-3 kg. The animals from the experimental groups were implanted with titanium pin, 3 mm long. The pin was implanted under combined anesthesia bilaterally into the body of the mandible. One day after the operation, the animals received SWT 500 pulses with a frequency of 5 Hz and a maximum pressure at the wavefront of 1.2 Bar per implantation site using the device Storz Medical Master Plus MP 100. The animals were removed from the experiment on 15-th day, after which the mandible was disarticulated and radiographically performed. The obtained results were statistically significant. The differences at p<0.05 were considered significant. Results. The results showed that the bone tissue of the jaws during implantation undergoes traumatic injury, the results of which, two weeks after surgery on radiographs we can clearly see a violation of the bone tissue structure and a significant increase in its density. Using the SWT method, we obtained a positive dynamics of bone density after titanium pin implantation and slight Rh-changes in bone structure compared with normal. Conclusion. Аfter implantation with titanium pin the bone density of the rabbit lower jaw body is significantly reduced, X-ray shows the heterogeneity of bone structure, areas of sclerosis are expressed. Shock wave therapy helps to restore the quality of bone tissue, this is confirmed by the fact that the density indicators are close to the norm and X-ray shows some separate small areas of restructuring of bone tissue heterogeneity, mainly due to merging the pattern of the bone trabeculae of the bone cancellous part.

scholarly journals Enhancing X-ray Attenuation of 3D Printed Gelatin Methacrylate (GelMA) Hydrogels Utilizing Gold Nanoparticles for Bone Tissue Engineering Applications

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 367 ◽  
Author(s):  
Nehar Celikkin ◽  
Simone Mastrogiacomo ◽  
X. Walboomers ◽  
Wojciech Swieszkowski
Keyword(s):  
Tissue Engineering ◽  
Gold Nanoparticles ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biological Properties ◽  
Proof Of Concept ◽  
New Bone Formation ◽  
X Ray ◽  
3D Printed ◽  
Low Toxicity

Bone tissue engineering is a rapidly growing field which is currently progressing toward clinical applications. Effective imaging methods for longitudinal studies are critical to evaluating the new bone formation and the fate of the scaffolds. Computed tomography (CT) is a prevailing technique employed to investigate hard tissue scaffolds; however, the CT signal becomes weak in mainly-water containing materials, which hinders the use of CT for hydrogels-based materials. Nevertheless, hydrogels such as gelatin methacrylate (GelMA) are widely used for tissue regeneration due to their optimal biological properties and their ability to induce extracellular matrix formation. To date, gold nanoparticles (AuNPs) have been suggested as promising contrast agents, due to their high X-ray attenuation, biocompatibility, and low toxicity. In this study, the effects of different sizes and concentrations of AuNPs on the mechanical properties and the cytocompatibility of the bulk GelMA-AuNPs scaffolds were evaluated. Furthermore, the enhancement of CT contrast with the cytocompatible size and concentration of AuNPs were investigated. 3D printed GelMA and GelMA-AuNPs scaffolds were obtained and assessed for the osteogenic differentiation of mesenchymal stem cells (MSC). Lastly, 3D printed GelMA and GelMA-AuNPs scaffolds were scanned in a bone defect utilizing µCT as the proof of concept that the GelMA-AuNPs are good candidates for bone tissue engineering with enhanced visibility for µCT imaging.

scholarly journals Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

2020 ◽  
Vol 21 (20) ◽  
pp. 7541
Author(s):  
Małgorzata Krok-Borkowicz ◽  
Katarzyna Reczyńska ◽  
Łucja Rumian ◽  
Elżbieta Menaszek ◽  
Maciej Orzelski ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Photoelectron Spectroscopy ◽  
Sessile Drop ◽  
Bone Tissue Regeneration ◽  
In Vivo Studies ◽  
In Vivo Model ◽  
Type I ◽  
X Ray

Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.

Mussel-inspired polydopamine-mediated surface modification of freeze-cast poly (ε-caprolactone) scaffolds for bone tissue engineering applications

2020 ◽  
Vol 65 (3) ◽  
pp. 273-287 ◽  
Author(s):  
Farnaz Ghorbani ◽  
Ali Zamanian ◽  
Melika Sahranavard
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Synergistic Effects ◽  
Engineering Application ◽  
Lamellar Microstructure ◽  
Tissue Engineering Application ◽  
Osteosarcoma Cell ◽  
Freeze Casting ◽  
X Ray

AbstractThere are many methods used to fabricate the scaffolds for tissue regeneration, among which freeze casting has attracted a great deal of attention due to the capability to create a unidirectional structure. In this study, polycaprolactone (PCL) scaffolds were fabricated by freeze-casting technology in order to create porous microstructure with oriented open-pore channels. To induce biomineralization, and to improve hydrophilicity and cell interactions, mussel-inspired polydopamine (PDA) was coated on the surface of the freeze-cast PCL constructs. Then, the synergistic effects of oriented microstructure and deposited layer on efficient reconstruction of injured bone were studied. Microscopic observations demonstrated that, the coated layer did not show any special change in lamellar microstructure of the scaffolds. Water-scaffold interactions were evaluated by contact angle measurements, and they demonstrated strong enhancement in the hydrophilicity of the polymeric scaffolds after PDA coating. Biodegradation ratio and water uptake evaluation confirmed an increase in the measured values after PDA precipitation. The biomineralization of the PDA-coated scaffolds was characterized by field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD). Obtained results confirmed biomineralization of the constructs after a 28-day immersion in a simulated body fluid (SBF) solution. Mechanical analysis demonstrated higher compressive strength after PDA coating. L929 fibroblast cell viability and attachment illustrated that PDA-coated PCL scaffolds are able to support cell adhesion and proliferation. The increased secretion of alkaline phosphatase (ALP) after culturing osteosarcoma cell lines (MG-63) revealed the initial capability of scaffolds to induce bone regeneration. Therefore, the PDA-coated scaffolds introduce a promising approach for bone tissue engineering application.

Bioactive Composite Materials for Bone Tissue Applications

2006 ◽  
Vol 514-516 ◽  
pp. 985-989
Author(s):  
B.J.M. Leite Ferreira ◽  
M.G.G.M. Duarte ◽  
M. Helena Gil ◽  
Rui N. Correia ◽  
J. Román ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bone Tissue ◽  
Tissue Repair ◽  
Body Fluid ◽  
In Vitro Bioactivity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biodegradable Composite ◽  
Scanning Electron

Two materials with potential application in bone tissue repair have been developed: 1) a non-biodegradable composite based in a new methacrylic-co-acrylic matrix; and 2) a biodegradable composite based in a chitosan (Ch) matrix. Both matrices were reinforced with glass-ceramic particles of composition (mol%) 70 SiO2 – 30 CaO. The in vitro bioactivity of composites was assessed by soaking in simulated body fluid (SBF) for periods of up to 7 days at 37º C. X-ray diffraction (XRD) and scanning electron microscopy coupled with X-ray energy dispersive spectroscopy (SEM-EDS) were used for deposit identification after different soaking periods. Calcium phosphate particulate deposits were detected after 3 days of immersion, followed by growth and maturation towards apatite.

Interferometric and X-ray arsorption studies of bone tissue

1954 ◽  
Vol 7 (1) ◽  
pp. 243-255 ◽  
Author(s):  
H DAVIES ◽  
A ENGSTROM
Keyword(s):  
Bone Tissue ◽  
X Ray
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