Salt-tolerance in Brassica juncea L. II. Salt-stress induced changes in polypeptide pattern of in vitro selected NaCl-tolerant plants

Euphytica ◽  
1993 ◽  
Vol 65 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Sunita Jain ◽  
H. S. Nainawatee ◽  
R. K. Jain ◽  
J. B. Chowdhury
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Brassica Juncea ◽  
Polypeptide Pattern ◽  
Tolerant Plants ◽  
Induced Changes

scholarly journals Agronomical, Physiological and Biochemical Characterization of In Vitro Selected Eggplant Somaclonal Variants under NaCl Stress

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2544
Author(s):  
Sami Hannachi ◽  
Stefaan Werbrouck ◽  
Insaf Bahrini ◽  
Abdelmuhsin Abdelgadir ◽  
Hira Affan Siddiqui
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Normal Growth ◽  
Nacl Stress ◽  
Soluble Carbohydrates ◽  
Regenerated Plants ◽  
And Control ◽  
Physiological And Biochemical

Previously, an efficient regeneration protocol was established and applied to regenerate plants from calli lines that could grow on eggplant leaf explants after a stepwise in vitro selection for tolerance to salt stress. Plants were regenerated from calli lines that could tolerate up to 120 mM NaCl. For further in vitro and in vivo evaluation, four plants with a higher number of leaves and longer roots were selected from the 32 plants tested in vitro. The aim of this study was to confirm the stability of salt tolerance in the progeny of these four mutants (‘R18’, ‘R19’, ‘R23’ and ‘R30’). After three years of in vivo culture, we evaluated the impact of NaCl stress on agronomic, physiological and biochemical parameters compared to the parental control (‘P’). The regenerated and control plants were assessed under in vitro and in vivo conditions and were subjected to 0, 40, 80 and 160 mM of NaCl. Our results show significant variation in salinity tolerance among regenerated and control plants, indicating the superiority of four regenerants (‘R18’, ‘R19’, ‘R23’ and ‘R30’) when compared to the parental line (‘P’). In vitro germination kinetics and young seedling growth divided the lines into a sensitive and a tolerant group. ‘P’ tolerate only moderate salt stress, up to 40 mM NaCl, while the tolerance level of ‘R18’, ‘R19’, ‘R23’ and ‘R30’ was up to 80 mM NaCl. The quantum yield of PSII (ΦPSII) declined significantly in ‘P’ under salt stress. The photochemical quenching was reduced while nonphotochemical quenching rose in ‘P’ under salt stress. Interestingly, the regenerants (‘R18’, ‘R19’, ‘R23’ and ‘R30’) exhibited high apparent salt tolerance by maintaining quite stable Chl fluorescence parameters. Rising NaCl concentration led to a substantial increase in foliar proline, malondialdehyde and soluble carbohydrates accumulation in ‘P’. On the contrary, ‘R18’, ‘R19’, ‘R23’ and ‘R30’ exhibited a decline in soluble carbohydrates and a significant enhancement in starch under salinity conditions. The water status reflected by midday leaf water potential (ψl) and leaf osmotic potential (ψπ) was significantly affected in ‘P’ and was maintained a stable level in ‘R18’, ‘R19’, ‘R23’ and ‘R30’ under salt stress. The increase in foliar Na+ and Cl− content was more accentuated in parental plants than in regenerated plants. The leaf K+, Ca2+ and Mg2+ content reduction was more aggravated under salt stress in ‘P’. Under increased salt concentration, ‘R18’, ‘R19’, ‘R23’ and ‘R30’ associate lower foliar Na+ content with a higher plant tolerance index (PTI), thus maintaining a normal growth, while foliar Na+ accumulation was more pronounced in ‘P’, revealing their failure in maintaining normal growth under salinity stress. ‘R18’, ‘R19’, ‘R23’ and ‘R30’ showed an obvious salt tolerance by maintaining significantly high chlorophyll content. In ‘R18’, ‘R19’, ‘R23’ and ‘R30’, the enzyme scavenging machinery was more performant in the roots compared to the leaves. Salt stress led to a significant augmentation of catalase, ascorbate peroxidase and guaiacol peroxidase activities in the roots of ‘R18’, ‘R19’, ‘R23’ and ‘R30’. In contrast, enzyme activities were less enhanced in ‘P’, indicating lower efficiency to cope with oxidative stress than in ‘R18’, ‘R19’, ‘R23’ and ‘R30’. ACC deaminase activity was significantly higher in ‘R18’, ‘R19’, ‘R23’ and ‘R30’ than in ‘P’. The present study suggests that regenerated plants ‘R18’, ‘R19’, ‘R23’ and ‘R30’ showed an evident stability in tolerating salinity, which shows their potential to be adopted as interesting selected mutants, providing the desired salt tolerance trait in eggplant.

scholarly journals Responses of own-rooted grape cultivars to salt stress in vivo and in vitro

2020 ◽  
pp. 199-209
Author(s):  
Ирина Ильинична Рыфф ◽  
Светлана Петровна Березовская
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Leaf Area ◽  
Grape Juice ◽  
Testing System ◽  
Potted Plants ◽  
Shoot Number ◽  
Grape Cultivars

В связи с усиливающейся засоленностью почв в мировом масштабе возникает настоятельная потребность в методах тестирования солетолерантности у сельскохозяйственных культур. Цель настоящего исследования заключалась в оценке толерантности трех корнесобственных сортов винограда к хлориду натрия в условиях in vitro и в вегетационной культуре. Солевой стресс in vitro моделировали добавлением в среду NaCl в концентрациях 0, 50 и 100 mM. В качестве индикаторов солетолерантности использовали площадь листьев и общую длину корней. Солевой стресс in vivo обеспечивался орошением в течение 75 дней водой с содержанием NaCl в концентрациях 0, 80, 100 and 120 mM. Солетолерантность растений оценивали на основании агробиологических параметров (площадь листьев, длина побегов, нагрузка побегами, одревеснение побегов) и анализом урожая (характеристики гроздей, урожай с куста, массовая концентрация сахаров, титруемая кислотность и pH сока). Также определяли водные потенциалы листьев как показатели водного баланса растений и электропроводность почвы в качестве показателя засоленности. Реакции на солевой стресс растений, выращиваемых in vitro и в горшечной культуре, продемонстрировали полную корреляцию. Increasing soil salinity on a global level gives rise to an imperative need for methods to test salt tolerance in crops. This study aimed to evaluate the tolerance of three own-rooted grape cultivars to potassium chloride both in an in vitro testing system and potted culture. Salt stress in vitro was modeled by treating in vitro-grown plants with NaCl at 0, 50, and 100 mM. Leaf area and total root length were used as indicators of salt tolerance. Salt stress in potted culture was achieved by irrigation for 75 days with water containing NaCl at 0, 80, 100, and 120 mM. Salt tolerance of potted plants was evaluated by parameters of viticultural performance (leaf area, shoot length, shoot number, shoot lignification), yield (characteristics of bunches, yield per plant, sugars, titratable acids and pH of grape juice). Leaf water potentials as a measure of the water balance of the plants and electrical conductivity of the soil as another indicator of salinity were also determined. Responses of in vitro-grown and potted plants to salt stress correlated closely.

Salt Stress-Induced Changes in In Vitro Cultured Stevia rebaudiana Bertoni: Effect on Metabolite Contents, Antioxidant Capacity and Expression of Steviol Glycosides-Related Biosynthetic Genes

2019 ◽  
Vol 38 (4) ◽  
pp. 1341-1353 ◽  
Author(s):  
Simone Ribeiro Lucho ◽  
Marcelo Nogueira do Amaral ◽  
Priscila Ariane Auler ◽  
Valmor João Bianchi ◽  
María Ángeles Ferrer ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Capacity ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Biosynthetic Genes ◽  
Stevia Rebaudiana Bertoni ◽  
Induced Changes

Why do plants lack sodium pumps and would they benefit from having one?

2017 ◽  
Vol 44 (5) ◽  
pp. 473 ◽  
Author(s):  
Jesper T. Pedersen ◽  
Michael Palmgren
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
First Generation ◽  
Normal Growth ◽  
Growth Conditions ◽  
Salt Tolerant ◽  
Tolerant Plants ◽  
Stress Related Genes ◽  
New Generation ◽  
High Degree

The purpose of this minireview is to discuss the feasibility of creating a new generation of salt-tolerant plants that express Na+/K+-ATPases from animals or green algae. Attempts to generate salt-tolerant plants have focussed on increase the expression of or introducing salt stress-related genes from plants, bryophytes and yeast. Even though these approaches have resulted in plants with increased salt tolerance, plant growth is decreased under salt stress and often also under normal growth conditions. New strategies to increase salt tolerance are therefore needed. Theoretically, plants transformed with an animal-type Na+/K+-ATPase should not only display a high degree of salt tolerance but should also reduce the stress response exhibited by the first generation of salt-tolerant plants under both normal and salt stress conditions. The biological feasibility of such a strategy of producing transgenic plants that display improved growth on saline soil but are indistinguishable from wild-type plants under normal growth conditions, is discussed.

scholarly journals Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro

2005 ◽  
pp. 51-55
Author(s):  
Pál Pepó ◽  
Szilárd Tóth
Keyword(s):  
Salt Stress ◽  
Solanum Tuberosum ◽  
Salt Tolerance ◽  
Callus Induction ◽  
Solanum Tuberosum L ◽  
Potato Production ◽  
Nacl Concentration ◽  
Callus Initiation ◽  
Selection Of

Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase

scholarly journals Response of Lettuce Germplasm to Salt Stress at Different Developmental Stages

2021 ◽  
Vol 6 (5) ◽  
Author(s):  
Pavli OI ◽  
◽  
Kempapidis K ◽  
Maggioros L ◽  
Foti C ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Growth Potential ◽  
Economic Losses ◽  
Saline Soils ◽  
Growth Stages ◽  
Salt Tolerant ◽  
Whole Plant ◽  
Plant Level

Salinity is one of the most detrimental abiotic stresses leading to considerable yield and economic losses worldwide. Lettuce is a relatively salt sensitive species, thus placing the interest in the release of salt-tolerant cultivars to enhance production in saline soils. This study aimed at investigating the response of lettuce germplasm to salt stress at the germination and at the whole plant level and to examine possibilities of early selection for salt tolerant genotypes. Fifteen lettuce commercial varieties were initially screened for salt tolerance on the basis of seed germination and seedling growth potential under salt stress conditions (0, 50, 100, 150 mM NaCl). The in vitro evaluation revealed the existence of considerable genetic variation related to salt tolerance at germination and allowed for the classification of genotypes into tolerant, moderately tolerant and sensitive to salt stress. Based on this classification, six cultivars were assessed at the whole plant level using plant height, chlorophyll content and fresh and dry biomass weight as evaluation criteria. Overall findings point to the existence of a satisfactory association of genotype performance between germination and later growth stages, thus suggesting the feasibility of screening for salt tolerance at early growth stages. This approach may considerably upgrade the efficiency of selecting suitable germplasm material for cultivation in saline soils or introgression into relevant breeding programs.

Sign in / Sign up

Export Citation Format

Share Document