Virus-specific proteins and RNAs from cells infected with hemorrhagic fever with renal syndrome (HFRS) viruses derived from endemic areas of the U.S.S.R.

1988 ◽  
Vol 102 (1-2) ◽  
pp. 147-154 ◽  
Author(s):  
I. S. Lukashevich ◽  
E. A. Tkachenko ◽  
N. N. Lemeshko ◽  
G. V. Rezapkin ◽  
T. A. Stelmakh ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Specific Proteins ◽  
Endemic Areas

scholarly journals TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA NYAMUKAEDES SPP.

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Angle M. H. Sorisi
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Dengue Hemorrhagic Fever ◽  
Virus Transmission ◽  
Hemorrhagic Fever ◽  
Health Problems ◽  
Transovarial Transmission ◽  
Blood Sucking ◽  
Endemic Areas ◽  
And Control

Abstract: Dengue Hemorrhagic Fever (DHF) is one of the most serious health problems in Indonesia which often causes outbreaks with numerous deaths. The disease is transmitted byAedes sp.females. Generally, dengue virus transmission occurs horizontally from human carriers, and the dengue viruses are passed on bytheir vectors through blood sucking activity. After propagation in the mosquito, the viruses are transmitted to human recipients. In addition, there is a vertical transmission (transovarial) of dengue virusesin the ova of Aedes sp.females. The viruses propagate in the ova that undergo  metamorphosis to become larvae, pupae, and imagoes. The transovarial transmission of dengue virusesin its vectors in endemic areas could be a causative key which is responsible for the phenomenon of increasing cases of DHF. Any effort to prevent and control DHF requires a thorough understanding about virDen transmission, including this transovarial transmission in Aedes spfemales. Keywords: DHF, transovarial transmission, Aedes sp.     Abstrak: Penyakit Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan yang semakin serius di Indonesia dan sering menimbulkan suatu Kejadian Luar Biasa (KLB) dengan jumlah kematian tinggi. Penyakit ditularkan melalui Aedes sp.betina. Transmisi virus dengue umumnya terjadi secara horizontal, yaitu dari manusia pembawa virus dengue ke nyamuk vektor Aedes sp. melalui aktivitasnya mengisap darahSetelah mengalami propagasi  dalam  tubuh nyamuk, virus dengue ditularkan ke  manusia penerima. Selain itu, transmisi virus dapat terjadi secara vertikal (transovarial) yaitu virus dengue dalam tubuh nyamuk vektorAedes sp. betinake ovum, kemudian berpropagasi dalam ovum, larva, pupa, dan imago. Transmisi transovarial virus dengueke vektornya di daerah endemik bisa menjadi kunci penyebab yang bertanggung jawab terhadap fenomena peningkatan kasus deman berdarah dengue. Upaya pencegahan dan penanggulangan DBD memerlukan pengetahuan yang matang tentang adanya infeksi transovarial virDen pada nyamuk Aedes sp. Kata kunci : DBD, transmisi transovarial,  Aedes sp.

scholarly journals A CASE OF IMPORTED DENGUE HEMORRHAGIC FEVER IN OMSK

2012 ◽  
Vol 17 (1) ◽  
pp. 49-51
Author(s):  
A. D. Safonov
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Endemic Areas

The paper provides a clinical and laboratory description of a case of dengue hemorrhagic fever imported by our countryman from Thailand to Omsk. It shows that the hemorrhagic fever of the disease may be developed in Russian citizens during multiple visits to endemic areas.

scholarly journals Genovariation Study of Hantavirus in Main Endemic Areas of Hemorrhagic Fever with Renal Syndrome in Hebei Province, China

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159731 ◽  
Author(s):  
Qi Li ◽  
Yanan Cai ◽  
Yamei Wei ◽  
Xu Han ◽  
Zhanying Han ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Hebei Province ◽  
Endemic Areas

scholarly journals Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia

2018 ◽  
Vol 19 (1) ◽  
pp. 31-36 ◽  
Author(s):  
HASMIWATI HASMIWATI ◽  
SELFI RENITA RUSJDI ◽  
EKA NOFITA
Keyword(s):  
Aedes Aegypti ◽  
Novel Mutation ◽  
Hemorrhagic Fever ◽  
Gene Encoding ◽  
Development Of Resistance ◽  
West Sumatra ◽  
Primary Vector ◽  
Endemic Areas ◽  
Larval Susceptibility

Hasmiwati, Rusjdi SR, Nofita E. 2018. Detection of Ace-1 gene with insecticides resistance in Aedes aegypti populations from DHF-endemic areas in Padang, Indonesia. Biodiversitas 19: 31-36. Aedes aegypti is distributed widely in West Sumatra as a primary vector of Dengue hemorrhagic fever, especially in Padang City. Synthetic insecticide control is one currently used method to prevent mosquito-borne diseases. The extensive, long-term application of Temephos along with inappropriate dosages, have resulted in the development of resistance in Ae. aegypti populations. Mutation of the Ace-1 gene, encoding an acetyl cholinesterase, is one of the mechanisms that confer resistance to organophosphate (OP). The Temephos resistance status of Ae. aegypti in Padang city has not yet been studied. This study aimed to investigate the resistance status of Ae. aegypti and identify any possible mutation (s) of the Ace-1 gene in Padang city. Ae. aegypti samples were collected in five population in Padang city (Jati (JT), Gunung Pangilun (GP), Lubuk Minturun (LM), Korong Gadang (KG), and Bandar Buat (BB)). The larval susceptibility to Temephos was tested by larval bioassays with Temephos pestanal at 0.02 mg/L dosages. Larval susceptibility was determined by mortality percentage values. The relationship between Ace-1 genotypes and the resistant phenotype was analyzed by percentage of genotype frequency. Out of five populations, assessed by larval bioassays, JT and GP were resistant to Temephos; LM, KG, and BB were tolerant. A total of 50 individuals from larval bioassays were genotyped for Ace-1 gene. Our findings showed that Ace-1 was 495 bp in length. Mutation was not found in the G119S location but in the T506T location. Three alleles in T506T location were detected, including a wild type allele, TT (65.21%), and two mutant alleles, TA (26.08%), AA (8.69%). The use of Temephos showed that some Ae. aegypti populations were resistant, others were tolerant, but no population was vulnerable to Temephos. A novel mutation was detected as substitution in T506T location (ACT>ACA).

scholarly journals Distribusi Kasus Demam Berdarah Dengue dan Habitat Perkembangbiakan Nyamuk Aedes aegypti serta Indeks Pupa pada Daerah Endemis dan Non Endemis di Kabupaten Sumba Timur, Nusa Tenggara Timur

2020 ◽  
Vol 48 (3) ◽  
Author(s):  
Ruben Wadu Wila ◽  
Tri Baskoro Tunggul Satoto ◽  
Mujiyanto Mujiyanto
Keyword(s):  
Incidence Rate ◽  
Nearest Neighbor ◽  
Spatial Relationship ◽  
Hemorrhagic Fever ◽  
Threshold Value ◽  
Distribution Patterns ◽  
Nearest Neighbour ◽  
Descriptive Research ◽  
Cluster Distribution ◽  
Endemic Areas

Abstract Dengue Hemorrhagic Fever (DHF) in Indonesia has spread in 34 provinces and 463 districts/cities. The Incidence Rate (IR) of DHF in East Sumba Regency in 2016 was 28.84 and 2017 increased to 55.28/100,000 population. The descriptive research was to describe the pattern of the spread of DHF cases and the habitat for the proliferation of DHF vectors and index pupae in the DHF endemic and non-endemic areas.The results showed that the Nearest Neighbor Ratio value in endemic areas was 0.292003 and in non-endemic areas was 0.718375. Moreover, the house pupae index was 53.3% and 41.0%, respectively The number of pupae/houses in endemic areas was 16.9 pupae/house, and in non-endemic areas was 11.9 pupae per house. The average pupae in each container in endemic areas were 3.6 pupae/container and sporadic areas were 2.2 pupae/container. The pupae/person value in endemic areas was 3.5 pupae/person and non-endemic areas was 2.2 pupae/person. The study concluded that there was a spatial relationship between DHF cases and DHF vector habitat with cluster distribution patterns. The number of pupae per house and pupae per person is still above the threshold value for DHF transmission in both endemic and non endemic areas. Keywords: DHF, endemicity, habitat, pupae index, East Sumba Abstrak DBD di Indonesia telah menyebar di 34 provinsi dan 463 kabupaten/Kota. Incidence Rate (IR) DBD pada tahun 2015 sebesar 89,37/100.000 penduduk dan menurun pada tahun 2016 menjadi 78,85/100.000 penduduk. Incidence Rate (IR) DBD di Kabupaten Sumba Timur tahun 2016 sebesar 28,84 dan 2017 meningkat menjadi 55,28/100.000 penduduk. Tujuan penelitian deskriptif ini adalah untuk menggambarkan pola penyebaran kasus DBD dan habitat perkembangbiakan vektor DBD serta pupa indeks di daerah endemis dan non endemis DBD, Kabupaten Sumba Timur, NTT. Hasil penelitian menunjukkan nilai Nearest Neighbour Ratio daerah endemis sebesar 0,292003, dan pada daerah non endemis sebesar 0,718375. House pupae index pada daerah endemis sebesar 53,3 % dan daerah non endemis 41,0%. Jumlah pupae/rumah pada daerah endemis lebih besar yaitu 16,9 pupae/rumah dan daerah non endemis sebanyak 11,9 pupae per rumah. Rata-rata pupae pada setiap container pada daerah endemis sebanyak 3,6 pupae/container dan daerah sporadis sebesar 2,2 pupae/container. Nilai pupae/person pada daerah endemis sebesar 3,5 pupae/person dan daerah non endemis 2,2 pupae/person. Kesimpulan penelitian yaitu terdapat hubungan spasial antara kasus DBD dan habitat vektor DBD dengan pola penyebaran cluster. Jumlah pupa per rumah dan pupa per orang masih di atas nilai ambang batas penularan DBD baik pada daerah endemis maupun non endemis. Kata kunci: DBD endemisitas, habitat, pupa indeks, Sumba Timur

scholarly journals Suggestive Serological Evidence of Infection with Shrew-Borne Imjin Virus (Hantaviridae) in Humans

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1128
Author(s):  
Rui Qi ◽  
Xi-Feng Sun ◽  
Xiang-Rong Qin ◽  
Li-Jun Wang ◽  
Min Zhao ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Sandwich Elisa ◽  
Hemorrhagic Fever ◽  
Healthy Individuals ◽  
Serological Evidence ◽  
Dot Blot ◽  
Human Sera ◽  
Normal Human ◽  
Endemic Areas ◽  
Dot Blot Analysis

The pathogenicity of the shrew-borne Imjin virus (MJNV) is unknown. The objective of our study was to find serological evidence of MJNV infection in humans. Partial MJNV nucleocapsid protein (NP) was cloned and expressed as an antigen for double-antigen sandwich ELISA, IgM capture ELISA, and dot blot to detect MJNV specific antibodies in hemorrhagic fever with renal syndrome (HFRS) patients’ and healthy persons’ sera from endemic areas in China. The purified recombinant NP reacted with neither the 90 healthy individuals’ sera from non-endemic areas of MJNV nor the 100 antisera to HFRS-causing virus, indicating that the MJNV NP had no cross-reaction with normal human sera and HFRS-causing viral antibodies. As determined by screening ELISA and dot blot analysis, IgG antibodies against MJNV NP were detected in sera from two of 385 healthy individuals from MJNV-endemic areas, suggesting infection with MJNV or MJNV-like thottimvirus. Based on the suggestive evidence, healthcare workers should be alert to febrile diseases occurring among individuals with exposure to shrew-infested habitats.

Knowledge, Attitude, and Practice of Dengue Hemorrhagic Fever Prevention Among Mothers in Endemic and Non-Endemic Locations of Pekanbaru City, Riau Province, Indonesia

2020 ◽  
Vol 1 (1) ◽  
pp. 55-66
Author(s):  
Tyagita Widya Sari ◽  
Martha Saptariza Yuliea ◽  
Novita Meqimiana Siregar ◽  
Raudhatul Muttaqin
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Health Centre ◽  
Sampling Technique ◽  
Hemorrhagic Fever ◽  
Bivariate Analysis ◽  
P Value ◽  
Cross Sectional ◽  
Attitude And Practice ◽  
Endemic Areas ◽  
Knowledge Attitude And Practice

Dengue hemorrhagic fever (DHF) is an infectious disease caused by dengue virus, which is one of the serious public health problems in Indonesia, particularly Pekanbaru City, Riau Province, Indonesia. One of the DHF endemic locations in Pekanbaru City is Payung Sekaki Health Centre, where 52 DHF cases and no deaths were reported in 2018. The number of DHF cases has increased to 53 and caused 1 death in January-August 2019 period (CFR = 1.89%). Karya Wanita Rumbai Health Centre is one of the DHF non-endemic areas in Pekanbaru City, where only 10 DHF cases and no deaths were reported in January-August 2019 period. The purpose of this study was to compare the knowledge, attitude, and practice of DHF prevention between mothers in endemic and non-endemic areas of Pekanbaru City, Riau Province, Indonesia. The design of this study was observational, with a cross-sectional approach. The sampling technique used in this study was accidental sampling, which included 100 respondents from each region. The data source of this study consisted of primary and secondary data. Data analysis was performed using bivariate analysis with Mann Whitney statistical test because the data were not normally distributed. The results of the study showed that there were no differences in knowledge (p-value = 0.912) and attitude (p-value = 0.065) of DHF prevention between mothers living in the endemic and non-endemic areas of Pekanbaru City, Riau Province, Indonesia. However, there were differences in practice of DHF prevention between mothers (p-value = 0.002) living in the endemic and non-endemic areas of Pekanbaru City, Riau Province, Indonesia. The conclusion of this study is that there is no difference in knowledge and attitude of DHF prevention between mothers living in the endemic and non-endemic areas, but there are differences in DHF prevention practice between mothers living in the endemic and non-endemic areas of Pekanbaru City, Riau Province, Indonesia.

Community Vulnerability Map in Endemic Areas of Dengue Hemorrhagic Fever (DHF), Banyumas, Indonesia

2020 ◽  
Author(s):  
Isna HIKMAWATI ◽  
Umi SHOLIKHAH ◽  
Hendro WAHJONO ◽  
Martini MARTINI
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Control Design ◽  
Hemorrhagic Fever ◽  
Case Control ◽  
Community Vulnerability ◽  
Mosquito Breeding ◽  
Vulnerability Map ◽  
Knowledge And Practice ◽  
Endemic Areas ◽  
The Relationship

Background: Community vulnerability is influenced by the low participation of the community in Pemberantasan Sarang Nyamuk (PSN). PSN is an activity done by society independently in their respective environment to eliminate mosquito-breeding places by 3 M (Menguras, Menutup, Mengubur). We aimed to prove the relationship between knowledge and practice of the community in PSN with the incidence of Dengue Hemorrhagic Fever (DHF), beside to describe the map of vulnerability of the community in endemic areas of DHF. Methods: This study used case control design. The population of this research is the community in the area of Puskesmas Kembaran 1 and 2. The samples were taken from Bojongsari villageas, Banyumas districtis, Indonesia one of the endemic areas of DHF from Jan 2014- Dec 2015. The number of samples was 62 respondents. Results: The community in endemic areas of knowledge about dengue was mostly good (55%) but in practice PSN was mostly less (56%). There was no correlation of knowledge with the incidence of DHF (P=0.444) and there was an association of DHF occurrence with PSN practice (P=0.010) and the vulnerability map showing many negative DHF residents living close to dengue cases. Conclusion: Community vulnerability in DHF endemic areas is dominated by densely populated settlements, Slum environmental conditions, and PSN practices are lacking. Mobilization of all components of the communityis required to participate in prevention of DHF.

BSL4 Facilities in Anti-Infectious Disease Measures

2009 ◽  
Vol 4 (5) ◽  
pp. 352-355 ◽  
Author(s):  
Ichiro Kurane ◽  
Keyword(s):  
Infectious Disease ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Lassa Virus ◽  
South American ◽  
Environment Effect ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Multiple Factors ◽  
Endemic Areas

Pathogens are divided into biosafety levels (BSL) 1 to 4 based on multiple factors such as virulence, transmissibility, environment effect, and treatment availability. BSL1 pathogens are the least virulent and BSL4 the most. BSL4 pathogens include ebolavirus, marburgvirus, Crimean-Congo hemorrhagic fever virus, lassa virus, variolla virus, and South American hemorrhagic fever viruses, as detailed in Table 1. Pathogens at each of the 4 BSLs must be handled in equivalently physically contained laboratories, graded P1-4. BSL4 pathogens do not exist in nature in Japan, which currently has no equivalent physical containment facilities, but the possibility exists that they may be brought into the country unintentionally by those infected in endemic areas or intentionally by bioterrorists.

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