Impact of Zinc Excess on Germination, Growth Parameters and Oxidative Stress of Sweet Basil (Ocimum basilicum L.)

Author(s):  
Hela Mahmoudi ◽  
Imene Ben Salah ◽  
Wafa Zaouali ◽  
Walid Zorrig ◽  
Ameni Smaoui ◽  
...  
2011 ◽  
Vol 57 (No. 4) ◽  
pp. 153-159 ◽  
Author(s):  
S. Ali ◽  
F. Zeng ◽  
S. Cai ◽  
B. Qiu ◽  
G. Zhang

The effect of chromium and NaCl on growth and anti-oxidative enzymes in two barley genotypes differing in salt tolerance was investigated in a hydroponic experiment. Salinity stress reduced plant growth, photosynthetic rate and stomatal conductance, while increased SOD and POD activities, and MDA content in barley plants. CM72, a salt-tolerant genotype was less affected by salinity stress than Gairdner, a salt-sensitive genotype. The effect of Cr on plant growth and anti-oxidative enzymes varied with Cr level. Under low Cr level (10 µmol), plant growth inhibition and oxidative stress caused by salinity stress were generally alleviated, in particular for Garidner. The combined stress of high Cr level (50 µmol) and NaCl stress caused more severe oxidative stress, resulting in further reduction of plant growth parameters, photosynthetic rate and stomatal conductance as compared to two stresses alone.


2015 ◽  
Vol 95 (4) ◽  
pp. 689-701 ◽  
Author(s):  
Samia Ageeb Akladious ◽  
George Saad Isaac ◽  
Medhat Ahmed Abu-Tahon

Akladious, S. A., Isaac, G. S. and Abu-Tahon, M. A. 2015. Induction and resistance against Fusarium wilt disease of tomato by using sweet basil (Ocimum basilicum L) extract. Can. J. Plant Sci. 95: 689–701. The antifungal activity of Ocimum basilicum (sweet basil) extract against Fusarium oxysporum f. sp. lycopersici race 3 the causal agent of tomato wilt and its ability in inducing disease resistance were studied in vivo using seed-soaking treatment before sowing. Plants were harvested at 45 and 105 d (vegetative and flowering stages) after sowing. Treatment with O. basilicum extract decreased the disease incidence from 94.70 to 18.00%. Results revealed that growth parameters and photosynthetic pigments were markedly inhibited in tomato plants in response to Fusarium wilt disease, whereas the contents of non-enzymatic and enzymatic antioxidants were increased as compared with healthy control plants. Moreover, presoaking in basil extract enhanced all the mentioned parameters in both healthy and infected plants. SDS-PAGE analysis of tomato leaves revealed that seed treated with basil extract resulted in an induction of novel protein bands during the vegetative stage. These new proteins were not detected in untreated healthy or infected control plants. Electrophoretic studies of polyphenol oxidase, esterase and malate dehydrogenase isoenzymes showed wide variations in their intensities and densities among all treatments. It seems that O. basilicum extract was able to enhance the biological control of Fusarium wilt disease of tomato.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1117
Author(s):  
Nadia de la Portilla ◽  
Rocio Vaca ◽  
Martha E. Mora-Herrera ◽  
Liliana Salinas ◽  
Pedro del Aguila ◽  
...  

We investigated changes in biochemical activity of a soil cultivated with Ocimum basilicum L. plants and in the oxidative stress of plants caused by the addition of biosolids and inorganic fertilizer. A greenhouse experiment was set up; it consisted of pots containing basil plants to which different treatments with biosolids and fertilizers were added. After 10 and 210 days of the incorporation of the treatments, both the biochemical properties and oxidative stress of the plants were evaluated. Mineralization of nitrogen (N) and respiratory activity increased with a higher dose of biosolid after 10 days of application. After 210 days, there were no changes in the mineralization of N and respiratory activity, catalase showed an increase, while microbial biomass carbon decreased. In relation to the oxidative stress, there was a decrease in the anthocyanin antioxidant capacity, and 2,2-diphenyl-1-picrylhydrazyl (DPPH), total flavonoids, and phenolic compounds were observed in soils treated with biosolids. The results indicated that the biochemical properties of the soil were favored in the presence of biosolids because they increased, especially after 10 days of application. The antioxidant capacity decreased in the presence of biosolids, which suggests that the amendment does not affect such capacity, and therefore oxidative stress could be reduced.


Botany ◽  
2013 ◽  
Vol 91 (7) ◽  
pp. 442-448 ◽  
Author(s):  
Saeid Zare Dehabadi ◽  
Abdolhamid Shoushtari ◽  
Zahra Asrar

Coronatine (COR), a functional analogue of jasmonates, affects the accumulation of defense-related metabolites in plants. However, information on how its effects may be mediated by heavy metal stress is scanty. This study was carried out to determine the changes in some physiological parameters in sweet basil (Ocimum basilicum L.) seedlings pretreated with COR under arsenic (As) toxicity. COR was applied to seedlings at the three-leaf stage at 50 and 100 nanomolars (nmol·L−1). Arsenic at 150 and 300 μmol·L−1 was imposed after completion of COR treatment. Toxic effects of As were reflected by the reduction in growth parameters and photosynthetic pigments and induction of lipid peroxidation coupled with enhancing hydrogen peroxide and electrolyte leakage in leaves. Under As toxicity, plants treated with COR significantly increased the fresh mass and relative water content in leaves. The increase was accompanied by decreased lipid peroxidation and hydrogen peroxide content and maintenance of chlorophyll pigments. COR supplement also increased the accumulation of phenolic compounds and proline content in the leaf of As-treated seedlings. Pretreatment with COR significantly enhanced activities of catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) in basil leaves under As treatment. The results suggested that COR might alleviate As toxicity in sweet basil by activating antioxidant enzymes and inducing conservative components, thereby preventing membrane peroxidation.


Author(s):  
Hatice Tunca

Toxic stress caused by pesticides changes the function and structure of the aquatic ecosystem via impressing to species composition. Therefore it is necessary to determine the reaction of cyanobacteria to pesticides for comprehend the effects of these substances on the aquatic ecosystems. This study aims to determine the toxicity and oxidative stress that Lambda cyhalothrin may cause in cyanobacteria, one of the primary producers in lake ecosystems. For these reasons, the changes in chlorophyll-a content, OD560 absorbance, the antioxidant enzyme acitvities such as superoxidedismutase (SOD), ascorbate peroxidase (APX), and glutathione reducatse (GR) were assessed to carry out the effect of Lambda cyhalothrin concentrations (between 6.25 and 100 μg ml−1) on Arthrospira platensis. EC50 value is calculated as 11.94 μg m l−1 Lambda cyhalothrin concentrations. SOD and APX activities was statistically different from the control at 100 μg m l−1 Lambda cyhalothrin application compared to control in A. platensis-M2 cells. On the other hand, GR activity did not effect significantly. According to our results, we may conclude that Lambda cyhalothrin concentrations used in this study inhibited the growth of A. platensis cells in a time and dose-dependent manner, as indicated by lowered chlorophyll-a content and OD560 values and Lambda cyhalothrin caused oxidative stress in A. platensis cells. As a result, the restriction of Lambda cyhalothrin using at the certain concentrations may be a step to prevent pesticide pollution in the environment.


2014 ◽  
Vol 6 (4) ◽  
pp. 422-427 ◽  
Author(s):  
Szidónia KŐSZEGHI ◽  
Csaba BERECZKI ◽  
Adalbert BALOG ◽  
Klára BENEDEK

Micropropagation of aromatic plants reveals an effective way of obtaining high volume, virus-free plant material of uniform quality. The application of meta-Topolin (mT) (N6-(2-hydroxybenzyl) adenine-9-riboside) and aromatic cytokinin as Benzyladenine (BAP) in the micro propagation of sweet basil (Ocimum basilicum L.) was tested for the first time and plant growth parameters assessed to determine the optimum level of these cytokinins. Additionally, the rate of root-growth inhibition due to these two cytokinins was also assessed. Our results show that 1 mg/l (4.43 mM) BAP and 0.5 mg/l (2.07 mM)mT produced the most favourable effects on new shoot developments. Meta-Topolin was shown to increase the quality of the plants and in comparison with BAP fewer distortions were observed. No significant differences in root-growth inhibition between the mT and BAP were detected.  


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