Antagonistic Activity of Lactic Acid Bacteria Against Phytopathogenic Fungi Isolated from Cherry Tomato (Solanum lycopersicum var. cerasiforme)

2021 ◽  
Author(s):  
Juan J. Manjarres Melo ◽  
Alejandro Álvarez ◽  
Cristina Ramirez ◽  
German Bolivar
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Solanum Lycopersicum ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Cherry Tomato

Antagonistic Effect of Lactic Acid Bacteria and their Consortium with Yeast on Pathogenic Microorganisms

2021 ◽  
Vol 37 (5) ◽  
pp. 96-107
Author(s):  
Е.F. Semenova ◽  
G.P. Zaitsev ◽  
Е.А. Slastya ◽  
А.V. Omelchenko ◽  
I.A. Bugara ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Capric Acid ◽  
Dimethyl Fumarate ◽  
Culture Liquid ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Fungistatic Activity

Abstract-A comparative study of the influence of lactic acid bacteria and their consortium with yeast on the growth of pathogenic bacteria and fungi has been carried out. It was revealed that the proposed composition of probiotic microbial species has fungistatic activity and strongly pronounced fungicidal activity against mold micromycetes and phytopathogenic fungi, respectively. The consortium effectiveness in suppressing both bacteria and filamentous fungi exceeds that of the corresponding monocultures. Analysis of wheat seeds for the presence of fungi by germination in a humid chamber and on nutrient media showed that the association of a 3-day culture of lactic acid bacteria and yeast at a dilution of 1:100 significantly reduced the infection of plant material. The ability to suppress opportunistic and phytopathogenic microorganisms is explained by the presence in the microbial consortium culture liquid of metabolites with bactericidal and fungicidal properties: squalene, dimethyl fumarate, capric acid, lactic acid, acetic acid, caprylic acid, fumaric acid, butyric acid, decanol, butanol, pentanol and β-phenylethanol. Key words: lactic acid bacteria, consortium of microorganisms, antagonistic activity, phytopathogens, bacteria pathogenic for humans

DEVELOPMENT OF A MULTIWELL ANTAGONISTIC ACTIVITY ASSAY FOR THE DETECTION OF BACTERIOCIN PRODUCTION BY LACTIC ACID BACTERIA

2009 ◽  
Vol 17 (1) ◽  
pp. 32-45 ◽  
Author(s):  
KAOUTAR YAAKOUBI ◽  
NOREDDINE BENKERROUM ◽  
FLORENT WIOROWSKI ◽  
FRANÇOISE SANSON ◽  
JULIEN HAYDERSAH ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antagonistic Activity ◽  
Bacteriocin Production ◽  
Activity Assay

Spray-Drying of Bacteriocin-Producing Lactic Acid Bacteria

1999 ◽  
Vol 62 (7) ◽  
pp. 773-777 ◽  
Author(s):  
GIANLUIGI MAURIELLO ◽  
MARIA APONTE ◽  
ROSAMARIA ANDOLFI ◽  
GIANCARLO MOSCHETTI ◽  
FRANCESCO VILLANI
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Cell Survival ◽  
Spray Drying ◽  
Antagonistic Activity ◽  
Cellular Damage ◽  
Bacteriocin Activity ◽  
Lactobacillus Curvatus ◽  
Significant Difference ◽  
Inhibitory Spectrum

Cell survival, cellular damage, and antagonistic activity were investigated after spray-drying of four bacteriocin-producing strains of lactic acid bacteria: Lactococcus lactis subsp. lactis 140, isolated from natural whey culture and producing a narrow-inhibitory spectrum bacteriocin); L. lactis subsp. lactis G35, isolated from pizza dough and producing nisin; Lactobacillus curvatus 32Y and Lactobacillus sp. 8Z, isolated from dry sausages. Trials were performed with bacteria suspended in skimmed milk or directly grown in whey. Three air temperatures at the inlet of the drier (160, 180, and 200°C) and three flow rates (10, 13, and 17 ml/min) were assayed. Cell viability and bacteriocin activity of the dried materials were determined immediately after the process and after 5, 15, 30, and 60 days of storage at 4°C. There was no significant difference between the two feeding suspensions in cell survival, always decreasing with the increase of inlet-air temperature. No loss of bacteriocin activity was detected in reconstituted powders, nor was any loss of ability to produce bacteriocin found after drying. Investigations of sensitivity to NaCl revealed only temporary damage to dried bacteria. During storage for 2 months at 4°C, all samples, but mainly the lactococcal strains, displayed a gradual decrease in cell survival. Bacteriocin activity remained at the same level, allowing powders to be considered as effective biopreservatives.

scholarly journals ISOLATION, SCREENING, AND CHARACTERIZATION OF PROBIOTICS (LACTIC ACID BACTERIA) ANTAGONISTIC AGAINST Candida albicans

2017 ◽  
Vol 4 (2) ◽  
pp. 263
Author(s):  
Ida Ayu Ketut Ariningsih ◽  
Yan Ramona ◽  
Nyoman Semadi Antara
Keyword(s):  
Candida Albicans ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Reproductive Tract ◽  
Antagonistic Activity ◽  
Low Ph ◽  
Production Test ◽  
Ph Conditions ◽  
High Level

Candidacies in female reproductive tract are mainly caused by Candida albicans. This infection often causes serious problems, particularly on their reproductive tract (genital part). Until recently, control of this infection has relied on the use of antibiotics. However due to numerous bad side effects of antibiotics, lactic acid bacteria have been proposed as an alternative method to control the growth of Candida albicans. Therefore, this research was aimed to isolate, screen, and characterize lactic acid bacterial isolates (LAB) antagonistic against Candida albicans (the causative agent of candidacies infection in reproductive tract of human). LABs were isolated from various fermented foods, such as tape ketan and kimchi. Isolation of LABs was conducted by applying dilution and spread plate method on MRS agar medium supplemented with BCP indicator to distinguish LABs from non acid-producing bacteria. Colonies with indication to produce acid were screened for antagonistic activity against C. albicans on MRS agar and followed by characterization of those isolates (Gram stain, catalase production test, oxydase production, gas production test, resistance test to low pH conditions and to high level of NaDC (sodium deoxicolic), and test for ability to convert colic acid (CA) into deoxicolic acid (DCA)). The results showed that 46 LAB isolates were successfully isolated from samples of tape ketan and kimchi. Among those, 7 isolates showed antagonistic activity against C. albicans in in vitro tests. All these 7 candidates were also found to be resistance to low pH conditions (up to pH 2) and to high level of NaDC (up to 0.6 mM). Four most potential isolates were further testes for ability to convert colic acid into deoxycolic acid and none showed positive result, indicating that they all showed initial potential and safe for future human probiotic development (especially to be used to treat patients infected by C. albicans).

scholarly journals Lactic acid bacteria isolated from chicken carcasses with inhibitory activity against Salmonella spp. and Listeria monocytogenes

2014 ◽  
Vol 32 (No. 1) ◽  
pp. 61-68 ◽  
Author(s):  
I. Sakaridis ◽  
N. Soultos ◽  
Ch. Batzios ◽  
I. Ambrosiadis ◽  
P. Koidis
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Inhibitory Activity ◽  
Antagonistic Activity ◽  
Growth Reduction ◽  
Salmonella Spp ◽  
Chicken Skin ◽  
Protective Culture

Lactic acid bacteria (LAB) isolated from poultry carcasses were added to BHI broth along with Salmonella spp. and Listeria monocytogenes in order to determine their antagonistic activity against the pathogens. There was a statistically significant reduction in Salmonella population on the 5<sup>th</sup> day that varied from 0.41 to 1.12 log CFU/ml. The reduction in L.&nbsp;monocytogenes population was also statistically significant and varied from 0.77 to 1.48 log CFU/ml. The LAB strain with the best inhibitory activity was chosen to examine its action against the same pathogens on the chicken skin and meat. On the chicken skin, the growth reduction on the 6<sup>th</sup> day caused by L. salivarius was lower and did not exceed the 0.54 log CFU/cm<sup>2</sup> for Salmonella spp. and 0.71 log CFU/cm<sup>2</sup> for L. monocytogenes. The reduction on the chicken meat was slightly lower for both pathogens. The results of the experiments suggest that L. salivarius (strain LAB 59) has a potential to be used as a protective culture to improve the safety and extend the shelf life of chicken products. &nbsp;

Sign in / Sign up

Export Citation Format

Share Document