Selectivity of a bromoacridine-containing fluorophore for triplex DNA
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AbstractFluorophore 1,8-naphthilamide was linked to 2-bromoacridine through an ethylenediamine spacer using a succinct synthetic route to give a bromoacridine-linked, bifunctional fluorophore conjugate for the detection of triplex DNA. Acridine is well known to intercalate into duplex DNA whereas introduction of a bulky bromine atom at position C2 redirects specificity for triplex over duplex DNA. In this work, photoelectron transfer assay was used to demonstrate that the synthesised 2-bromoacridine-linked fluorophore conjugate had good selectivity for the representative triplex DNA target sequence d(T*A.T)20 compared with double-stranded d(T.A)20, single-stranded dT20 or d(G/A)19 DNA sequences. Graphic abstract
2017 ◽
Vol 114
(21)
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pp. 5443-5448
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2010 ◽
Vol 08
(02)
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pp. 181-198
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2015 ◽
Vol 43
(2)
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pp. 147
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1993 ◽
Vol 13
(5)
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pp. 2697-2705
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2020 ◽
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