A biochemiluminescent assay for rapid diagnosis of influenza

1984 ◽

Vol 42 ◽

pp. 226-227 ◽

Cited By ~ 1

Author(s):

K. Pegg-Feige ◽

F. W. Doane

Keyword(s):

Electron Microscopy ◽

Cell Culture ◽

Immunoelectron Microscopy ◽

Detection Method ◽

Solid Phase ◽

Protein A ◽

Plant Viruses ◽

Rapid Diagnosis ◽

Virus Diagnosis ◽

Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

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99mTc Technetium Sulfide and the Scintillation Camera in the Rapid Diagnosis of Pericardial Effusion

Nuklearmedizin ◽

10.1055/s-0038-1624740 ◽

1971 ◽

Vol 10 (02) ◽

pp. 122-128

Author(s):

W. H. Blahd ◽

M. A. Winston ◽

G. T. Krishnamurthy ◽

P. B. Thomas ◽

E. Weiss

Keyword(s):

Pericardial Effusion ◽

Patient Positioning ◽

Rapid Diagnosis ◽

Scintillation Camera ◽

Technical Error ◽

Technetium 99M ◽

Sodium Pertechnetate ◽

Specific Uptake ◽

Speed Accuracy

SummaryBecause of its speed, accuracy, and reproducibility, radioisotopic angiocardiography appears to be the method of choice for the diagnosis of pericardial effusion. Technetium-99m sulfide is preferable to 99mTc sodium pertechnetate in these studies for the following reasons: (1) its specific uptake by the liver aids both in patient positioning and in the accuracy of diagnosis; and (2), studies can be repeated within 15—20 minutes should there be equiment or technical error.

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Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v6i1.19354 ◽

2012 ◽

Vol 6 (1) ◽

pp. 11-13

Author(s):

Sushmita Roy ◽

S.M. Shamsuzzaman ◽

K.Z. Mamun

Keyword(s):

Stool Sample ◽

Rapid Detection ◽

Cross Sectional Study ◽

Rapid Diagnosis ◽

Age Group ◽

College Hospital ◽

Cross Sectional ◽

Rotavirus Infection ◽

Medical College ◽

Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

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