Criblage phytochimique et activité antimicrobienne des extraits de Thymus serpyllum et de Thymus vulgaris du mont de Tessala (Algérie occidentale)

The TLC essential oils profiles were being used conventionally for identification of herbal raw materials (HRM) of Lamiaceae family. Despite this, in recent years the European Pharmacopoeia to call in question the appropriateness of TLC on essential oils determination of Thyme and Wild Thyme, as well as Peppermint and Peppermint dry extract; these methods were replaced by HPTLC using ﬂavonoid proﬁle. This work aimed to establish the possibility of conducting HPTLC identification of methanolic extracts of 13 members of the Lamiaceae family in harmonized chromatographic conditions using chromatographic profiles on essential oils. The objects were methanolic extracts of HRM of Ukraine flora – Salvia officinalis, Salvia aethiopis, Thymus serpyllum, Thymus vulgaris, Origanum vulgare, Rosmarinus officinalis, Ocimum tenuiflorum (foreign origin), Mentha × piperita, Melissa officinalis, Orthosiphon stamineus, Agastache foeniculum, Leonurus cardiaca, Hyssopus officinalis, Marrubium vulgare, prepared from HRM that were purchased in Ukraine. The study wоas done by standardized HPTLC procedure using automatic HPTLC herbal system of CAMAG, Switzerland, at the base of CAMAG laboratory, Muttenz, Switzerland, and laboratory of ESTCCTI ESIPP, NUPh, Ukraine. Chromatography conditions: stationary phase – HPTLC plates Si 60 F254; mobile phase – toluene-ethylacetate (95:5); reference standards – borneol, bornyl acetate, cineole; derivatization – anisaldehyde reagent; detection: WRT, 254, 366 nm – before derivatization; WRT, 366 nm – after derivatization; sample preparation – methanolic extracts (100 mg/ml), extracted by shaking technique. In unified chromatographic conditions the HPTLC chromatographic fingerprints of essential oils of 13 Lamiaceae family species were established and evaluated. The most specific, intensive and reproducible HPTLC fingerprints were for Sage and Rosemary extracts. Moreover, fingerprints of Sage, Thyme, Rosemary, Origanum, Ocimum and Peppermint shown specific zones useful for differentiation of different genera of Lamiaceae family. For setting relative acceptance criteria for each of the species the validation for specific zones that were detected should be done.

Download Full-text

PLANTS OF THE BOTSAD OF I.M. SECHENOV MOSCOW STATE MEDICAL UNIVERSITY, CAPABLE OF SUPPRESSING DENSITY-DEPENDENT COMMUNICATION OF MICROORGANISMS.

10.26897/978-5-9675-1855-3-2021-101 ◽

2021 ◽

Author(s):

O. A. Karashchuk ◽

Keyword(s):

Botanical Garden ◽

Thymus Vulgaris ◽

Chelidonium Majus ◽

Botanic Garden ◽

Calendula Officinalis ◽

Inula Helenium ◽

Comarum Palustre ◽

Betula Verrucosa ◽

Thymus Serpyllum ◽

Medical University

Purpose of research - specify plants in the Botanic garden with antiquorum activity. In the collection of the botanical garden there are plants with confirmed antiquorum activity: Quercus robur, Betula verrucosa, Ledum tomentosum, Salvia officinalis, Inula helenium, Calendula officinalis, Comarum palustre, Rosa majalis. The presence of anti-Quorum Sensing activity supposed in: Chelidonium majus, Maсleaya cordata, Ruta graveolens, Plantago major, Equisetum arvense, Thymus serpyllum, Thymus marschallianus, Thymus vulgaris. The use of drugs from these plants led to the elimination of Stenotrophomonas maltophilia in the patient.

Download Full-text

Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

Open Chemistry ◽

10.1515/chem-2020-0011 ◽

2020 ◽

Vol 18 (1) ◽

pp. 108-118 ◽

Cited By ~ 1

Author(s):

Tomasz Baj ◽

Anna Biernasiuk ◽

Rafał Wróbel ◽

Anna Malm

Keyword(s):

Candida Albicans ◽

Chemical Composition ◽

Antifungal Activity ◽

Essential Oils ◽

Thymus Vulgaris ◽

Origanum Vulgare ◽

Satureja Hortensis ◽

Broth Microdilution Method ◽

Thymus Serpyllum

AbstractThe purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were analyzed using the GC-MS method. The antifungal activity was evaluated in vitro against oral isolates and reference strains of Candida albicans and C. glabrata, using the broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) guidelines, allowing for estimation of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). GC-MS analysis revealed that carvacrol was the main EO compound in oregano and summer savory, while thymol and linalool were the major ingredients of thyme and wild thyme oils, respectively. The EOs possessed fungicidal activity against C. albicans and C. glabrata, including oral isolates, with MIC = 125 – 2000 mg/L, MFC = 250 – 4000 mg/L and MFC/MIC = 1 – 4, depending on the yeast and plant species. The most active was thyme oil – with MIC = 125 – 500 mg/L, MFC = 250 – 500 mg/L and MFC/MIC = 1 – 2.

Download Full-text

A Validation Model for Prediction of Kovats Retention Indices of Compounds Isolated from Origanum spp. and Thymus spp. Essential Oils

Journal of the Mexican Chemical Society ◽

10.29356/jmcs.v65i4.1515 ◽

2021 ◽

Vol 65 (4) ◽

Author(s):

Ivana Čabarkapa ◽

Milica Aćimović ◽

Lato Pezo ◽

Vanja Tadić

Keyword(s):

Essential Oils ◽

Prediction Models ◽

Retention Indices ◽

Thymus Vulgaris ◽

Quantitative Structure ◽

Origanum Vulgare ◽

Kováts Retention Indices ◽

Structure Retention ◽

Thymus Serpyllum ◽

Quantitative Structure Retention Relationship

Abstract. This work aimed to obtain a validated model for the prediction of retention times of compounds isolated from Origanum heracleoticum, Origanum vulgare, Thymus vulgaris, and Thymus serpyllum essential oils. In total 68 experimentally obtained retention times of compounds, which were separated and detected by GC-MS were further used to build the prediction models. The quantitative structure–retention relationship was employed to foresee the Kovats retention indices of compounds acquired by GC-MS analysis, using eight molecular descriptors selected by a genetic algorithm. The chosen descriptors were used as inputs for the four artificial neural networks, to construct a Kovats retention indices predictive quantitative structure–retention relationship model. The coefficients of determination in the training cycle were 0.830; 0.852; 0.922 and 0.815 (for compounds found in O. heracleoticum, O. vulgare, T. vulgaris and T. serpyllum essential oils, respectively), demonstrating that these models could be used for prediction of Kovats retention indices, due to low prediction error and high r2. Resumen. El objetivo de este trabajo es la obtención de modelos validados para la predicción del tiempo de retención de los compuestos aislados de aceites esenciales de Origanum heracleoticum, Origanum vulgare, Thymus vulgaris y Thymus serpyllum. Se han obtenido un total de 68 tiempos de retención de compuestos, separándose y detectándose por cromatografía de gases con detección por espectrometría de masas (GC-MS) con posterior desarrollo de modelos de predicción. La relación cuantitativa estructura-retención ha sido utilizada para predecir el índice de retención Kovats de los compuestos obtenidos por análisis de GC-MS, utilizando ocho descriptores moleculares seleccionados mediante algoritmo genético. Los descriptores seleccionados han sido utilizados como entrada para las cuatro redes neuronales artificiales y así elaborar los índices predictivos del modelo de relación cuantitativa estructura-retención. Los coeficientes de determinación en el ciclo de entrenamiento fueron de 0.830; 0.852; 0.922 y 0.815 (para los compuestos identificados en los aceites esenciales del O. heracleoticum, O. vulgare, T. vulgaris y T. serpyllum respectivamente) demostrando así que estos modelos son útiles en la predicción de los índices de retención de Kovats con un error de bajo predicción y alta r2.

Download Full-text

Sustainable One-Step Solid-State Synthesis of Antibacterially Active Silver Nanoparticles Using Mechanochemistry

Nanomaterials ◽

10.3390/nano10112119 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2119

Author(s):

Mária Kováčová ◽

Nina Daneu ◽

Ľudmila Tkáčiková ◽

Radovan Búreš ◽

Erika Dutková ◽

...

Keyword(s):

Silver Nanoparticles ◽

Solid State ◽

Antibacterial Properties ◽

Absorption Spectrometry ◽

Milling Process ◽

Thymus Vulgaris ◽

Tem Analysis ◽

One Step ◽

Thymus Serpyllum ◽

The Stability

A combination of solid-state mechanochemical and green approaches for the synthesis of silver nanoparticles (AgNPs) is explored in this study. Thymus serpyllum L. (SER), Sambucus nigra L. (SAM) and Thymus vulgaris L. (TYM) plants were successfully applied to reduce AgNO3 to AgNPs, as confirmed by X-ray diffraction analysis, with SER being the best reducing agent, and TYM being the worst. The experiments were performed via a one-step planetary milling process, where various AgNO3:plant mass ratios (1:1, 1:10, 1:50 and 1:100) were investigated. Atomic absorption spectrometry indicated that the stability of the mechanochemically produced AgNPs increased markedly when a sufficiently large quantity of the reducing plant was used. Furthermore, when larger quantities of plant material were employed, the crystallite size of the AgNPs decreased. TEM analysis revealed that all AgNPs produced from both AgNO3:plant ratios 1:1 and 1:10 exhibit the bimodal size distribution with the larger fraction with size in tens of nm and the smaller one below 10 nm in size. The antibacterial activity of the produced AgNPs was observed only for AgNO3:plant ratio 1:1, with the AgNPs prepared using SER showing the greatest antibacterial properties.

Download Full-text