scholarly journals Comparison of chromatographic profiles of essential oils in extracts of 13 species from Lamiaceae family

2020 ◽  
pp. 86-94
Author(s):  
K. O. Khohklova ◽  
L. I. Vyshnevska ◽  
O. A. Zdoryk
Keyword(s):  
Essential Oils ◽  
Raw Materials ◽  
Mentha Piperita ◽  
Bornyl Acetate ◽  
Thymus Vulgaris ◽  
Methanolic Extracts ◽  
Marrubium Vulgare ◽  
Ocimum Tenuiflorum ◽  
Thymus Serpyllum ◽  
Chromatographic Profiles

The TLC essential oils profiles were being used conventionally for identification of herbal raw materials (HRM) of Lamiaceae family. Despite this, in recent years the European Pharmacopoeia to call in question the appropriateness of TLC on essential oils determination of Thyme and Wild Thyme, as well as Peppermint and Peppermint dry extract; these methods were replaced by HPTLC using flavonoid profile. This work aimed to establish the possibility of conducting HPTLC identification of methanolic extracts of 13 members of the Lamiaceae family in harmonized chromatographic conditions using chromatographic profiles on essential oils. The objects were methanolic extracts of HRM of Ukraine flora – Salvia officinalis, Salvia aethiopis, Thymus serpyllum, Thymus vulgaris, Origanum vulgare, Rosmarinus officinalis, Ocimum tenuiflorum (foreign origin), Mentha × piperita, Melissa officinalis, Orthosiphon stamineus, Agastache foeniculum, Leonurus cardiaca, Hyssopus officinalis, Marrubium vulgare, prepared from HRM that were purchased in Ukraine. The study wоas done by standardized HPTLC procedure using automatic HPTLC herbal system of CAMAG, Switzerland, at the base of CAMAG laboratory, Muttenz, Switzerland, and laboratory of ESTCCTI ESIPP, NUPh, Ukraine. Chromatography conditions: stationary phase – HPTLC plates Si 60 F254; mobile phase – toluene-ethylacetate (95:5); reference standards – borneol, bornyl acetate, cineole; derivatization – anisaldehyde reagent; detection: WRT, 254, 366 nm – before derivatization; WRT, 366 nm – after derivatization; sample preparation – methanolic extracts (100 mg/ml), extracted by shaking technique. In unified chromatographic conditions the HPTLC chromatographic fingerprints of essential oils of 13 Lamiaceae family species were established and evaluated. The most specific, intensive and reproducible HPTLC fingerprints were for Sage and Rosemary extracts. Moreover, fingerprints of Sage, Thyme, Rosemary, Origanum, Ocimum and Peppermint shown specific zones useful for differentiation of different genera of Lamiaceae family. For setting relative acceptance criteria for each of the species the validation for specific zones that were detected should be done.

scholarly journals In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 272 ◽  
Author(s):  
Ramona Iseppi ◽  
Alessandro Di Cerbo ◽  
Piero Aloisi ◽  
Mattia Manelli ◽  
Veronica Pellesi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Disk Diffusion ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Extended Spectrum ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), Melaleuca alternifolia, Eucalyptus globulus, Mentha piperita, and Thymus vulgaris, in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and carbapenemase (KPC)-producing Klebsiella pneumoniae. A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing E. coli, 5 K. pneumoniae, 13 KPC-producing K. pneumoniae, and 20 MBL-producing P. aeruginosa were cultured and reconfirmed as ESBL and carbapenamase producers. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (ESBL and KPC/MBL). Antibacterial activity of the EOs was determined using the agar disk diffusion assay, and minimal inhibitory concentrations (MICs) were also evaluated. Lastly, adhesion capability and biofilm formation on polystyrene and glass surfaces were studied in 24 randomly selected strains. M. alternifolia and T. vulgaris EOs showed the best antibacterial activity against all tested strains and, as revealed by agar disk diffusion assay, M. alternifolia was the most effective, even at low concentrations. This effect was also confirmed by MICs, with values ranging from 0.5 to 16 µg/mL and from 1 to 16 µg/mL, for M. alternifolia and T. vulgaris EOs, respectively. The EOs’ antibacterial activity compared to antibiotics confirmed M. alternifolia EO as the best antibacterial agent. T. vulgaris EO also showed a good antibacterial activity with MICs lower than both reference antibiotics. Lastly, a significant anti-biofilm activity was observed for the two EOs (*P < 0.05 and **P < 0.01 for M. alternifolia and T. vulgaris EOs, respectively). A good antibacterial and anti-biofilm activity of M. alternifolia and T. vulgaris EOs against all selected strains was observed, thus demonstrating a future possible use of these EOs to treat infections caused by ESBL/carbapenemase-producing strains, even in association with antibiotics.

Activity of Essential Oils and Individual Components against Acetyland Butyrylcholinesterase

2008 ◽  
Vol 63 (7-8) ◽  
pp. 547-553 ◽  
Author(s):  
Ilkay Orhan ◽  
Murat Kartal ◽  
Yüksel Kan ◽  
Bilge Şener
Keyword(s):  
Essential Oils ◽  
Organic Fertilizer ◽  
Mentha Piperita ◽  
Cultivated Plants ◽  
Bornyl Acetate ◽  
Anethum Graveolens ◽  
Chemical Fertilizers ◽  
Foeniculum Vulgare ◽  
Lavandula Officinalis ◽  
Almost All

We have tested acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities of nineteen essential oils obtained from cultivated plants, namely one from Anethum graveolens L. (organic fertilizer), two from Foeniculum vulgare Mill. collected at fullymature and flowering stages (organic fertilizer), two from Melissa officinalis L. (cultivated using organic and chemical fertilizers), two from Mentha piperita L. and M. spicata L. (organic fertilizer), two from Lavandula officinalis Chaix ex Villars (cultivated using organic and chemical fertilizers), two from Ocimum basilicum L. (green and purple-leaf varieties cultivated using only organic fertilizer), four from Origanum onites L., O. vulgare L., O. munitiflorum Hausskn., and O. majorana L. (cultivated using organic fertilizer), two from Salvia sclarea L. (organic and chemical fertilizers), one from S. officinalis L. (organic fertilizer), and one from Satureja cuneifolia Ten. (organic fertilizer) by a spectrophotometric method of Ellman using ELISA microplate-reader at 1 mg/ml concentration. In addition, a number of single components widely encountered in most of the essential oils [γ-terpinene, 4-allyl anisole, (-)-carvone, dihydrocarvone, (-)-phencone, cuminyl alcohol, cumol, 4-isopropyl benzaldehyde, trans-anethole, camphene, iso-borneol, (-)-borneol, l-bornyl acetate, 2- decanol, 2-heptanol, methyl-heptanol, farnesol, nerol, iso-pulegol, 1,8-cineole, citral, citronellal, citronellol, geraniol, linalool, α-pinene, β-pinene, piperitone, iso-menthone, menthofurane, linalyl oxide, linalyl ester, geranyl ester, carvacrol, thymol, menthol, vanilline, and eugenol] was also screened for the same activity in the same manner. Almost all of the essential oils showed a very high inhibitory activity (over 80%) against both enzymes, whereas the single components were not as active as the essential oils.

scholarly journals Stabilisation potentials of the essential oils of Thymus vulgaris L., Cinnamomum zeylanicum B. and Mentha piperita L. on palm olein at accelerated storage

2020 ◽  
Vol 19 (7) ◽  
pp. 464-477
Author(s):  
Agwanande Ambindei Wilson ◽  
Michel Dongmo Jazet Pierre ◽  
Ngoune Tatsadjieu Leopold ◽  
P. Priya ◽  
P. Nisha
Keyword(s):  
Essential Oils ◽  
Palm Olein ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Cinnamomum Zeylanicum ◽  
Accelerated Storage

scholarly journals The Influence of Essential Oils on Gut Microbial Profiles in Pigs

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1734 ◽  
Author(s):  
Modestas Ruzauskas ◽  
Elena Bartkiene ◽  
Arunas Stankevicius ◽  
Jurga Bernatoniene ◽  
Daiva Zadeike ◽  
...  
Keyword(s):  
Essential Oils ◽  
Plant Extract ◽  
Basal Diet ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Metagenomic Sequencing ◽  
Microbial Composition ◽  
Animal Group ◽  
The Impact

In recent years, the intake of ultra-processed foods has increased dramatically worldwide. Missing natural foods in the diet raise the need of biologically active food components that could compensate for this deficiency and help maintain proper immune status. This study used pigs as an animal model for the assessment of the impact of consumption of Origanum vulgare plant extract combined with Mentha piperita and Thymus vulgaris essential oils on microbial profile in intestines. A single group of weaned pigs received basal diet, while the other group basal diet supplemented with plant extract and two essential oils in the form of bilayer tablets prepared using “liquid/solid” phase technology. Metagenomic sequencing was performed with the aim to investigate changes of microbial communities in ileum, caecum, and colon. The results demonstrated that the combination of essential oils was non cytotoxic, and had a positive effect on the microbial composition in the large intestine of pigs due to significant increase in the number of probiotic bacteria. The amount of Lactobacillus was 2.5 times and Bifidobacterium 1.9 times higher in the animal group fed with supplement. The combination, however, had some negative impact on the variety of minor species in the distal part of the ileum. Additional studies need to be performed to obtain knowledge on how combinations of essential oils can change bacterial variety in the proximal part of the gastrointestinal tract.

scholarly journals Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils

2014 ◽  
Vol 52 ◽  
pp. 183-190 ◽  
Author(s):  
Miloš Nikolić ◽  
Jasmina Glamočlija ◽  
Isabel C.F.R. Ferreira ◽  
Ricardo C. Calhelha ◽  
Ângela Fernandes ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antitumor Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Thymus Algeriensis ◽  
Thymus Serpyllum

scholarly journals Antifungal and Antitoxigenic Effects of Selected Essential Oils in Vapors on Green Coffee Beans with Impact on Consumer Acceptability

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2993
Author(s):  
Miroslava Hlebová ◽  
Lukas Hleba ◽  
Juraj Medo ◽  
Viktoria Uzsakova ◽  
Pavel Kloucek ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Sensory Profile ◽  
Aspergillus Ochraceus ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Cinnamon Bark ◽  
Toxigenic Fungi ◽  
Litsea Cubeba ◽  
Coffee Beans

The main objective of this study is to evaluate the effect of selected essential oils thyme chemotype linalool (Thymus zygis L.), thyme chemotype tymol (Thymus vulgaris L.), eucalyptus (Eucalyptus globulus Labill.), lavender (Lavandula angustifolia Mill.), mint (Mentha piperita L.), almond (Prunbus dulcis Mill.), cinnamon bark (Cinnamomum zeylanicum Nees), litsea (Litsea cubeba Lour. Pers), lemongrass (Cympogon citrati L. Stapf), and ginger (Zingiber officinalis Rosc.) in the vapor phase on growth, sporulation, and mycotoxins production of two Aspergillus strains (Aspergillus parasiticus CGC34 and Aspergillus ochraceus CGC87), important postharvest pathogens of green and roasted coffee beans. Moreover, the effect of the essential oils (EOs) on the sensory profile of the coffee samples treated with EOs was evaluated. The major components of tested EOs were determined by gas chromatography and mass spectrometry (GC–MS) and gas chromatography with flame ionization detector (GC-FID). The results showed that almond, cinnamon bark, lemongrass, and litsea EOs are able to significantly inhibit the growth, sporulation, and mycotoxins production by toxigenic fungi. Sensory evaluation of coffee beans treated with EOs before and after roasting showed that some EOs (except lemongrass and litsea) do not adversely affect the taste and aroma of coffee beverages. Thus, application of the vapors of almond and cinnamon EOs appears to be an effective way that could serve to protect coffee during its transport and storage from toxigenic fungi.

scholarly journals Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

2020 ◽  
Vol 18 (1) ◽  
pp. 108-118 ◽  
Author(s):  
Tomasz Baj ◽  
Anna Biernasiuk ◽  
Rafał Wróbel ◽  
Anna Malm
Keyword(s):  
Candida Albicans ◽  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Origanum Vulgare ◽  
Satureja Hortensis ◽  
Broth Microdilution Method ◽  
Thymus Serpyllum

AbstractThe purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were analyzed using the GC-MS method. The antifungal activity was evaluated in vitro against oral isolates and reference strains of Candida albicans and C. glabrata, using the broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) guidelines, allowing for estimation of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). GC-MS analysis revealed that carvacrol was the main EO compound in oregano and summer savory, while thymol and linalool were the major ingredients of thyme and wild thyme oils, respectively. The EOs possessed fungicidal activity against C. albicans and C. glabrata, including oral isolates, with MIC = 125 – 2000 mg/L, MFC = 250 – 4000 mg/L and MFC/MIC = 1 – 4, depending on the yeast and plant species. The most active was thyme oil – with MIC = 125 – 500 mg/L, MFC = 250 – 500 mg/L and MFC/MIC = 1 – 2.

scholarly journals Effects of Natural Antimicrobials on Xanthomonas Strains Growth

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Irena Mačionienė ◽  
Dovilė Čepukoit ◽  
Joana Šalomskienė ◽  
Darius Černauskas ◽  
Daiva Burokienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Lactobacillus Helveticus ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Natural Antimicrobials ◽  
Inhibition Zones

The aim of this work was to investigate the most promising natural antimicrobials effective for the growth suppression of Xanthomonas spp. bacteria. The research objects were Xanthomonas spp. strains isolated from tubers and stem of plants growing in Lithuania: Xanthomonas translucens NRCIB X6, X. arboricola NRCIB X7, NRCIB X8, NRCIB X9, and NRCIB X10; the supernatants of lactic acid bacteria Lactococcus lactis strains 140/2, 57, and 768/5, Lactobacillus helveticus strains 14, 148/3, R, and 3, Lb. reuteri 3 and 7, Streptococcus thermophilus 43, Enterococcus faecium 59-30 and 41-2; endophytic bacterial strains Bacillus, Pseudomonas, and Paenibacillus spp.; and essential oils of lavender (Lavandula angustifolia), grapefruit (Citrus paradisi), pine (Pinus sylvestris), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), peppermint (Mentha piperita), lemon (Citrus limetta), aqueous extracts of blueberries (Vaccinium myrtillus), and cranberries (Vaccinium vitis-idaea). The antimicrobial activity of tested substances was determined by agar diffusion method. Supernatants of Lb. reuteri strain 7 and Lb. helveticus strains 14, R, 3, and 148/3 were found to have a high antimicrobial activity against Xanthomonas spp. bacteria strains when compared to the positive control—1.0% copper sulfate (diameter of inhibition zones was 28.8 ± 0.7 mm). The diameter of inhibition zones of supernatants ranged from 23.3 ± 0.6 mm to 32.0 ± 0.1 mm. Thyme (2.0%) and lavender (2.0%) essential oils inhibited the growth of Xanthomonas spp. strains. The diameter of the inhibition zones was from 14.7 ± 0.8 mm to 22.8 ± 0.9 mm. The aqueous extracts of blueberries had a weak antimicrobial activity. The diameter of inhibition zones ranged from 11.0 ± 0.2 mm to 13.0 ± 0.2 mm.

scholarly journals Structural-functional reorganization of dimorphous black yeast-like fungi Exophiala alcalophila under influence of plant essential oils

2015 ◽  
Vol 70 (2) ◽  
pp. 42-46 ◽  
Author(s):  
T. Kondratiuk ◽  
A. Kalinichenko
Keyword(s):  
Essential Oils ◽  
Black Yeast ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Yeast Culture ◽  
Plant Essential Oils ◽  
Functional Reorganization ◽  
Wide Adaptation ◽  
High Level

Antifungal influence of essential oils of the following plants Cariophyllus aromaticus L., Foennicilum vulgare Mill., Lavandula anqustifolia Mill., Mentha piperita L., Melaleuca alternifolia Maid., Origanum vulgare L., Pelargonium roseum Wild., Thymus vulgaris L., and benzalconium chloride on black yeast-like fungi Exophiala alcalophila Goto et Sugiy was investigated. High level of the antifungal influence of plant oils of O. vulgare, Th. vulgaris, P. roseum and C. aromaticus was found with the usage of disc-diffusion method. The influence of plant essential oils mentioned is similar to influence of 3% benzalconium chloride. The following structural-functional reorganization of Exophiala alcalophila, i.e.: exchange of morphometric indices of cells, colony morphology, intensity of budding, dimorphous transition 'yeast-mycelium' were observed under influence of benzalconium chloride and plant essential oils of O. vulgare, Th. vulgaris, P. roseum and C. aromaticus. These exchanges illustrate wide adaptation possibilities of black yeast culture investigated.

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