Chitosan-Mediated Changes in dry Matter, Total Phenol Content and Essential Oil Constituents of two Origanum Species under Water Deficit Stress

2020 ◽  
Author(s):  
Hamid Mohammadi ◽  
Leila Aghaee Dizaj ◽  
Ahmad Aghaee ◽  
Mansour Ghorbanpour
2005 ◽  
Vol 11 (2) ◽  
Author(s):  
R. Engel ◽  
A. Hegedűs ◽  
Zs. Bodor ◽  
M. Heltmann-Tulok ◽  
É. Stefanovits-Bányai

The growth rate of three rosemary clones, `Harmat', 'Salem' and `Horvát', their essential oil content and its components as well as the total phenol content and total antioxidant capacity in their aqueous and ethanolic extracts were compaired. Total phenol content (determined by Folin-Ciocalteau reagent) and antioxidant power (FRAP-value) of aqueous and ethanolic rosemary extracts were measured by spectrophotometric method. The essential oil content was determined from drug by water-steam distillation. `Harmat' showed the most intensive growth through the vegetation period. The total antioxidant power of the ethanolic extracts from all the three clones was similar: a decrease was observed at the end of the season. In most cases more antioxidant compounds were dissolved in the aqueous extracts. The total quantity of phenolic compounds shows a good correlation with the potential extent of stress effects. The difference among the phenol contents of the various clones can be derived from the difference in their tolerance, because phenols have antioxidant effects contributing to protection against harmful impacts. Difference between the two extraction methods (aqueous and ethanolic) is due to the distinct solubility of compounds. Some other components, like essential oil compounds also possess antioxidant effect and in this way they may influence the antioxidant power of extracts.


2020 ◽  
Vol 15 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Housseyn Chaoua ◽  
Ihcen Khacheba ◽  
Hadjer Boussoussa ◽  
Abir Bekhaoua ◽  
Mohamed Yousfi

Background: Natural plant active compounds were found to inhibit the activity of several enzymes that may be related to several diseases. Objective: This study aimed at testing the antidiabetic activity related to the phenol content by in vitro α-Amylase inhibitory action effect of aqueous, organic and essential oil extracts of Haplophyllum tuberculatum, collected in the town of Laghouat in the steppe region of Algeria. Methods: Two types of aqueous extracts were prepared: Decoction and Diffusion extracts. The organic extracts were prepared with successful maceration in hexane, dichloromethane, ethyl acetate, ethanol and methanol. Also, essential oils were obtained by hydrodistillation. The analysis of the total Phenol content of our extracts was done with Folin-Ciocalteu reagent, as the flavonoid content was obtained in mixture with aluminum trichloride. The effects of the plant extracts on the catalytic efficiency of α-amylase enzyme were represented by the enzymatic inhibitory percentage of each extract in which the inhibitory activity was expressed as IC50. Results: The total phenol content showed values ranging between 0.27 and 11.97 mg gallic acid equivalent / g dry matter. The flavonoid contents vary from 0.05 to 1.50 mg equivalent of rutin /g of dry matter. All the extracts showed good inhibitory activity against α- amylase of IC50, values ranged from 0.05 to 50.03 mg/ml. Conclusion : This study reports for the first time the inhibitory capacity of Algerian Haplophyllum tuberculatum species against α-amylase which could provide natural biologically active agents to be used in the management of diabetes.


2007 ◽  
Vol 109 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Gabriel Beltrán ◽  
María T. Ruano ◽  
Antonio Jiménez ◽  
Marino Uceda ◽  
María P. Aguilera

2017 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Éva LASLO ◽  
Zoltán Attila KÖBÖLKUTI

This study evaluated the antimicrobial activity and the total phenol content of Vaccinium vitis idaea L. berry fruit from five different localities with distinct growth sites in the Eastern Carpathians. The antibacterial effect of lingonberry was studied on nine selected Gram-positive and negative, foodborne, illness causing and spoilage bacteria. The total phenol content was estimated by the Folin-Ciocalteu method. The present results showed stronger antibacterial effect of lingonberry on Gram-negative bacteria, especially on Pseudomonas fluorescens and Pseudomonas aeruginosa. The total phenol content varied between 3.72 and 2.1 mM GAE/ml. As data suggested, Vaccinium vitis-idaea fruits originating from different geographic regions and environment, differ from each other in terms of bioactive compound quantity and activity. In the selection of new perspective cultivars of lingonberry, the geographical origin of fruits must be considered. Two Step Cluster analysis detected relatively well supported relationship between samples provided from similar growth sites. Correlation analysis showed no correlation between altitude, phenol content and antimicrobial activity.


2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.


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