Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

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SKRINING FITOKIMIA DAN PENETAPAN KADAR FENOL TOTAL PADA EKSTRAK DAUN NANGKA (Artocarpus heterophyllus), CEMPEDAK (Artocarpus integer), dan TARAP (Artocarpus odoratissimus) ASAL DESA PENGARON KABUPATEN BANJAR

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v4i1.667 ◽

2021 ◽

Vol 4 (1) ◽

pp. 95-102

Author(s):

Muhammad Ikhwan Rizki ◽

◽

Nurlely Nurlely ◽

Fadlilaturrahmah Fadlilaturrahmah ◽

Ma’shumah Ma’shumah

Keyword(s):

Abdominal Pain ◽

Secondary Metabolites ◽

Total Phenol ◽

Total Phenol Content ◽

Phytochemical Screening ◽

Phenol Content ◽

Artocarpus Heterophyllus ◽

Phenol Group ◽

The People ◽

Leaf Powder

The leaves of jackfruit (Artocarpus heterophyllus), cempedak (Artocarpus integer), and tarap (Artocarpus odoratissimus) are used empirically by the people of Desa Pengaron, Banjar Regency for treat antimalarials, antidiabetic, abdominal pain, and cancer. The secondary metabolites of the phenol group are responsible for various plant activities. This study aims to determine of compounds and total phenol content in leaves of jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus). The leaf samples were oven-dried, then the leaf powder was extracted using ethanol. Phytochemical screening was carried out on the extract using reagents, and phenol content was determined using a spectrophotometer UV-Vis. The results showed that the extracts of leaves jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus) contained phenol, flavonoids, and tannins. Total phenol content of leaves A. heterophyllus, A. integer and A. odoratissimus were 13,174 ± 1,378 mgGAE / gram extract, 37,204 ± 2,202 mgGAE / gram extract, and 35,886 ± 0.890 mgGAE / gram extract.

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Synergism of Antioxidant Activity Combination of Buas-Buas (Premnaserratifolia Linn.), Meniran (Phyllanthusniruri L.), Secang (Caesalpiniasappan) and Roselle (Hibiscus sabdarifa) Extracts

Majalah Obat Tradisional ◽

10.22146/mot.51328 ◽

2020 ◽

Vol 25 (3) ◽

Author(s):

Isnindar Isnindar ◽

Sri Luliana

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Total Flavonoids ◽

Phenol Content ◽

Flavonoid Content ◽

Ic50 Values ◽

Phenolic And Flavonoid

Buas-buas, meniran, secang, and rosella have biological and pharmacological activities as antioxidants. The combination of the four plants is expected to provide a more potent synergistic effect on antioxidant activity. The purpose of this study was to analyze the total phenol content, total flavonoids, and antioxidant effects before and after combination. The combination of extracts, buas-buas, meniran, secang, and rosella which are used in sequence is (1: 1: 1/2: 1/2), (1: 2: 1/2: 1/2), and (2: 1 : 1/2: 1/2). Plants used in the form of simplicia was extracted by maceration method. Radical capture activity uses DPPH and IC50 values are determined. Determination of total phenol is expressed equivalent to gallic acid. Total flavonoids are expressed as quercetin equivalents. The phenol and flavonoid content obtained are then correlated with antiradical activity. The results showed that the best IC50 values were in the combination of ratios (1: 1: 1/2: 1/2) that is (11.0 µg / mL), then (1: 2: 1/2: 1/2) which was 13.3 µg / mL, and (2: 1: 1/2: 1/2) which is 19.4 µg / mL. The highest total phenolic and flavonoid content in the ratio (1: 2: 1/2: 1/2) is 33.57% w/w EAG and 74.00% w/w EQ. Correlation analysis between IC50 values with total phenolic and flavonoid contents showed a positive correlation with R2 values of 0.8236 and 0.0102 with positive slope. Thus, it can be concluded that the total phenol content influences free radical scavenging activity by 82.36%, while the effect of total flavonoid content was only 1.02%.

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Phenolic Composition and Antioxidant Activity of Peel, Pulp and Seed Extracts of Different Clones of the Turkish Grape Cultivar ‘Karaerik’

Plants ◽

10.3390/plants10102154 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2154

Author(s):

Muhammed Kupe ◽

Neva Karatas ◽

Mehmet Settar Unal ◽

Sezai Ercisli ◽

Mojmir Baron ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenol ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Content ◽

Grape Cultivar ◽

Seed Extracts

The Erzincan plain is one of the richest regions in Turkey in terms of plant biodiversity. In this region, the famous grape cultivar ‘Karaerik’ has always dominated grape production due to its berry characteristics. The cultivar shows great morphological variation at clonal level. In this study, the total phenolic content and antioxidant activity of peel, pulp and seed extracts of nine ‘Karaerik’ clones sampled from same location were investigated. The Folin–Ciocalteu method was used to determine the total phenolic content of peel, pulp and seed extracts of nine clones. To determine antioxidant activity, three well known assays such as DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity) were used. In addition, the correlation between total phenol content and DPPH, FRAP and TEAC was determined. Results showed that among the tissues, seed samples in berries of all clones had the highest total phenol content and antioxidant activity determined by three assays. Seed samples were followed by peel and pulp for total phenolic content and antioxidant activity. Among the nine ‘Karaerik’ clones, Clone 8 had the highest total phenolic content (149 mg GAE/100 g FW) while Clone 3 had the lowest (111 mg GAE/100 g FW). Peel, pulp and seed samples of nine ‘Karaerik’ clones showed strong antioxidant activity in DPPH, FRAP and TEAC assays. In particular, grape seeds were found rich for better in phenolic compounds including gallic acid, quercetin, catechin, chlorogenic acid, caffeic acid and p-coumaric acid. Clones such as 7, 8 and 9 higher antioxidant activity may present great potential for grape breeders and the food industry as well as health-conscious consumers.

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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽

10.35799/pha.9.2020.29268 ◽

2020 ◽

Vol 9 (2) ◽

pp. 178

Author(s):

Julia Elsa Lakoro ◽

Max R. J. Runtuwene ◽

Paulina V. Y. Yamlean

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Compounds ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of 53,34 mg / L. Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

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Prediction of Total Phenolic Content in Extracts of Prunella Species from HPLC Profiles by Multivariate Calibration

ISRN Chromatography ◽

10.5402/2012/120780 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Saliha Sahin ◽

Esra Isik ◽

Cevdet Demir

Keyword(s):

High Performance ◽

Multivariate Calibration ◽

Principal Component Regression ◽

Principal Component ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Content ◽

Calibration Methods ◽

Hplc Profiles

The multivariate calibration methods—principal component regression (PCR) and partial least squares (PLSs)—were employed for the prediction of total phenol contents of four Prunella species. High performance liquid chromatography (HPLC) and spectrophotometric approaches were used to determine the total phenol content of the Prunella samples. Several preprocessing techniques such as smoothing, normalization, and column centering were employed to extract the chemically relevant information from the data after alignment with correlation optimized warping (COW). The importance of the preprocessing was investigated by calculating the root mean square error (RMSE) for the calibration set of the total phenol content of Prunella samples. The models developed based on the preprocessed data were able to predict the total phenol content with a precision comparable to that of the reference of the Folin-Ciocalteu method. PLS model seems preferable, because of its predictive and describing abilities and good interpretability of the contribution of compounds to the total phenol content. Multivariate calibration methods were constructed to model the total phenol content of the Prunella samples from the HPLC profiles and indicate peaks responsible for the total phenol content successfully.

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Chestnut Drying Is Critical in Determining Aspergillus flavus Growth and Aflatoxin Contamination

Toxins ◽

10.3390/toxins10120530 ◽

2018 ◽

Vol 10 (12) ◽

pp. 530 ◽

Cited By ~ 6

Author(s):

Simona Prencipe ◽

Ilenia Siciliano ◽

Carlotta Gatti ◽

Maria Gullino ◽

Angelo Garibaldi ◽

...

Keyword(s):

Antioxidant Activity ◽

Fungal Growth ◽

Aflatoxin Production ◽

Aflatoxin Contamination ◽

Total Phenol ◽

Fungal Colonization ◽

Total Phenol Content ◽

Phenol Content ◽

Drying Treatment ◽

Drying Conditions

Chestnut drying is used to prevent postharvest losses and microorganism contamination during storage. Several studies reported the contamination by aflatoxins (AFs) produced by Aspergillus spp. in chestnuts. The effect of drying temperatures (from 30 to 50 °C) was evaluated on the growth of A. flavus and the production of aflatoxins in chestnuts. The influence of the treatment on the proximate composition, the total phenol content and antioxidant activity of chestnuts was considered. Fungal colonization was observed on the nuts dried at 30, 35, and 40 °C; the incidence was lower at 40 °C. The highest concentrations of AFB1 and AFB2 were produced at 40 °C. No aflatoxins were detected at 45 or 50 °C. At 40 °C A. flavus was under suboptimal conditions for growth (aw 0.78), but the fungus was able to synthesize aflatoxins. As the temperatures applied increased, the total phenol content increased, while the antioxidant activity decreased. A drying treatment at 45 °C for seven days (aw 0.64) could be a promising method to effectively control both the growth of aflatoxigenic fungi and the production of aflatoxins. This study provides preliminary data useful to improve the current drying conditions used in chestnut mills, to reduce both fungal growth and aflatoxin production.

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Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v39i1.28413 ◽

2017 ◽

Vol 39 (1) ◽

pp. 43 ◽

Cited By ~ 7

Author(s):

Mayara Neves Santos Guedes ◽

Rafael Pio ◽

Luana Aparecida Castilho Maro ◽

Fabíola Fonseca Lage ◽

Celeste Maria Patto de Abreu ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Phenolic Compounds ◽

High Performance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenol ◽

Total Phenol Content ◽

Phenol Content ◽

Phenolic Contents

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested.

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