Antioxidant enzymes as biochemical markers for sharka resistance in apricot

Developing traditional medicine in the field of hepatology and nephrology research is the key to pharmacology. This study demonstrates the mechanism of hepatoprotective and renal protective activity of Ficus dalhousiae Miq. (Moraceae) methanolic leaf extract on carbon tetrachloride induced hepatic and renal toxicity. Shade dried powder was subjected to shoxlet extraction with methanol and assessed for hepatoprotective and renal protective activities. Hepatotoxicity and renal toxicity were induced in rats by single oral dose of CCl4 diluted with olive oil (1:1 v/v; ml/kg body weight) after pretreatment of methanolic extract for seven days. Sixteen hrs after CCl4 administration, rats were sacrificed and biochemical markers like Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) estimated followed by the measurement of liver and kidney cytosolic antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and reactive oxygen species (ROS). The data were analysed by one way analysis of variance (ANOVA). The extract at the doses of 150 and mg/kg b.w. significantly reduces elevated levels of ALT, AST, ALP and LDH. The extracts also showed significant increase in the reduced level of SOD, CAT and GSH. The ROS activity also found down regulated. The activity of methanolic extracts were comparable with the standard Silymarin. These findings not only showed potential hepatoprotective and renal protective activities of Ficus dalhousiae but also manifested by restoring antioxidant enzymes. With this pilot study we can justify the medicinal importance of this plant.

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Effects of Bioactive Compounds from Rosemary on Biochemical Markers and Antioxidant Enzymes in Liver and Kidneys in Diabetic Animals

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2010.10.080 ◽

2010 ◽

Vol 49 ◽

pp. S39

Author(s):

Ana Mara de Oliveira e Silva ◽

Eliane Bonifácio Teixeira de Carvalho ◽

Milessa da Silva Afonso ◽

Illana Louise Pereira de Melo ◽

Jorge Mancini-Filho

Keyword(s):

Antioxidant Enzymes ◽

Bioactive Compounds ◽

Biochemical Markers

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The metabolic profile in laying hens of the Rosso race after 180 days of feed supplement with organic selenium

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine ◽

10.15835/buasvmcn-vm:12163 ◽

2016 ◽

Vol 73 (2) ◽

pp. 285

Author(s):

Adrian Raduta ◽

Dumitru Curca

Keyword(s):

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Glutathione Peroxidase ◽

Biochemical Markers ◽

Metabolic Profile ◽

Feed Supplement ◽

Organic Selenium ◽

Calcium Magnesium ◽

Experimental Group

Selenium is a trace mineral with antioxidant proprieties, which, by mediating the glutathione, indirectly protects the hemoglobin against the risk of oxidation by peroxides through three antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalaseThe experiment was done on 20 laying chicks from the Rosso race, 6 weeks old at the start. The chicks were divided in 2 batches, and one of the batches received feed enriched with organic selenium (Sel-Plex). After 180 days, biological samples were colected by cubital vein puncture and hematological and biochemical determinations were made. The results were tabled, graphically represented and biostatistically interpreted. In the experimental batch significant growths were observed in the erythrocyte constants: erithremie, hemoglobinemy, hematocrite and MCH. Of the biochemical markers significant growths were observed in: ascorbinemic acid, alkalin phospahasis and selenium. The following parameters dropped significantly : proteinemia, amylase and MCV. Changes were also observed in cholesterol, calcium, magnesium and other parameters, but without statisticaly value. In the present paper we have shown the beneficial role of organic selenium on some haematological and biochemical markers, resulting in a growth of erythropoiesis, and at the same time a intensification of the metabolic processes in the experimental group.

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Specific biochemical markers of bone metabolism and cytokine study confirm the diagnosis of malignant infantile osteopetrosis at birth using cord blood sample

Pathology ◽

10.1080/00313020400024725 ◽

2005 ◽

Vol 37 (1) ◽

pp. 51-55 ◽

Cited By ~ 3

Author(s):

Michael Chan ◽

Wong K. ◽

Iris Chan ◽

Y. F. Luo ◽

Sidney Tam ◽

...

Keyword(s):

Cord Blood ◽

Blood Sample ◽

Bone Metabolism ◽

Biochemical Markers ◽

Cord Blood Sample

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Effects of Antioxidant Supplementation and Exercise Training on Erythrocyte Antioxidant Enzymes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.76.5.324 ◽

2006 ◽

Vol 76 (5) ◽

pp. 324-331 ◽

Cited By ~ 11

Author(s):

Marsh ◽

Laursen ◽

Coombes

Keyword(s):

Antioxidant Enzymes ◽

Exercise Training ◽

Endurance Training ◽

Young Male ◽

Antioxidant Defenses ◽

Antioxidant Enzyme Activities ◽

Antioxidant Supplementation ◽

Male Wistar Rats ◽

Exercise Induced ◽

Antioxidant Supplements

Erythrocytes transport oxygen to tissues and exercise-induced oxidative stress increases erythrocyte damage and turnover. Increased use of antioxidant supplements may alter protective erythrocyte antioxidant mechanisms during training. Aim of study: To examine the effects of antioxidant supplementation (α-lipoic acid and α-tocopherol) and/or endurance training on the antioxidant defenses of erythrocytes. Methods: Young male Wistar rats were assigned to (1) sedentary; (2) sedentary and antioxidant-supplemented; (3) endurance-trained; or (4) endurance-trained and antioxidant-supplemented groups for 14 weeks. Erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, and plasma malondialdehyde (MDA) were then measured. Results: Antioxidant supplementation had no significant effect (p > 0.05) on activities of antioxidant enzymes in sedentary animals. Similarly, endurance training alone also had no effect (p > 0.05). GPX (125.9 ± 2.8 vs. 121.5 ± 3.0 U.gHb–1, p < 0.05) and CAT (6.1 ± 0.2 vs. 5.6 ± 0.2 U.mgHb–1, p < 0.05) activities were increased in supplemented trained animals compared to non-supplemented sedentary animals whereas SOD (61.8 ± 4.3 vs. 52.0 ± 5.2 U.mgHb–1, p < 0.05) activity was decreased. Plasma MDA was not different among groups (p > 0.05). Conclusions: In a rat model, the combination of exercise training and antioxidant supplementation increased antioxidant enzyme activities (GPX, CAT) compared with each individual intervention.

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Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000007 ◽

2010 ◽

Vol 80 (1) ◽

pp. 65-73 ◽

Cited By ~ 10

Author(s):

Pei-Min Chao ◽

Wan-Hsuan Chen ◽

Chun-Huei Liao ◽

Huey-Mei Shaw

Keyword(s):

Antioxidant Enzymes ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Thiobarbituric Acid ◽

Control Group ◽

Thiobarbituric Acid Reactive Substances ◽

Control Groups ◽

Protein Levels ◽

Transfer Protein ◽

And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

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Biochemical Markers of Brain Injury: An Integrated Proteomics-Based Approach

PsycEXTRA Dataset ◽

10.1037/e451452006-001 ◽

2005 ◽

Author(s):

Ronald L. Hayes

Keyword(s):

Brain Injury ◽

Biochemical Markers

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Results of study of biochemical markers of bone metabolism in men with coronary heart disease

Medical alphabet ◽

10.33667/2078-5631-2020-15-39-43 ◽

2020 ◽

pp. 39-43

Author(s):

A. V. Voronkina ◽

T. A. Raskina ◽

M. V. Letaeva ◽

Yu. V. Averkieva ◽

O. S. Malyshenko ◽

...

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Bone Formation ◽

Bone Metabolism ◽

Biochemical Markers ◽

Coronary Atherosclerosis ◽

Cathepsin K ◽

Arterial Calcification ◽

Mineral Density ◽

Bone Remodeling Markers

The development of atherosclerosis is closely related to the calcification of the vessel intima and fibrous plaques, being a complex and multifactorial process, in which the markers of bone formation and resorption play an important role. Objective. To study the biochemical markers of bone metabolism in men with stable coronary heart disease (CHD). Material and methods. The study included 102 men with verified CHD. Data were evaluated by densitometry, coronary angiography, multispiral computed tomography, color duplex scanning of brachiocephalic arteries, serum lipids (total cholesterol, triglycerides [TG], high-density [LHD] and low-density lipoprotein cholesterol), concentrationsin the blood of osteocalcin (OC), bone alkaline phosphatase (BAP), cathepsin K and C-telopeptides (CTx). Results. Concentrations of BAP, cathepsin K and CTx in patients with CHD were significantly higher than in men without CHD. The concentration of OC in men with normal bone mineral density was significantly lower than in patients with osteopenic syndrome. There was a direct correlation between OC and antiatherogenic HDL cholesterol and the inverse correlation between OC and TG, CTx and TG. There was no correlation between the level of bone remodeling markers and coronary artery (CA) lesion variant and the severity of coronary atherosclerosis on SYNTAX scale. The correlation analysis did not reveal the connection of biochemical markers of bone metabolism with the severity of coronary atherosclerosis and calcification and thickness of intima-media complex of carotid arteries. Absolute values of bone formation indices (BAP, OC) were significantly higher in patients with severe СA calcification than in patients without signs of calcification. Summary. Increased rates of osteogenesis and osteoresorption characterize the accelerated process of bone metabolism and indicate in favor of high rates of bone loss in men with CHD, which confirms the likelihood of common pathophysiological mechanisms of bone resorption and arterial calcification.

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