scholarly journals Development of bioinks for 3D printing microporous, sintered calcium phosphate scaffolds

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
Sergio A. Montelongo ◽  
Gennifer Chiou ◽  
Joo L. Ong ◽  
Rena Bizios ◽  
Teja Guda
Keyword(s):  
Calcium Phosphate ◽  
3D Printing ◽  
Mechanical Strength ◽  
Biomedical Applications ◽  
Alternative Methods ◽  
High Porosity ◽  
Calcium Phosphate Ceramic ◽  
3D Print ◽  
Beta Tricalcium Phosphate ◽  
Strength And Stiffness

AbstractBeta-tricalcium phosphate (β-TCP)-based bioinks were developed to support direct-ink 3D printing-based manufacturing of macroporous scaffolds. Binding of the gelatin:β-TCP ink compositions was optimized by adding carboxymethylcellulose (CMC) to maximize the β-TCP content while maintaining printability. Post-sintering, the gelatin:β-TCP:CMC inks resulted in uniform grain size, uniform shrinkage of the printed structure, and included microporosity within the ceramic. The mechanical properties of the inks improved with increasing β-TCP content. The gelatin:β-TCP:CMC ink (25:75 gelatin:β-TCP and 3% CMC) optimized for mechanical strength was used to 3D print several architectures of macroporous scaffolds by varying the print nozzle tip diameter and pore spacing during the 3D printing process (compressive strength of 13.1 ± 2.51 MPa and elastic modulus of 696 ± 108 MPa was achieved). The sintered, macroporous β-TCP scaffolds demonstrated both high porosity and pore size but retained mechanical strength and stiffness compared to macroporous, calcium phosphate ceramic scaffolds manufactured using alternative methods. The high interconnected porosity (45–60%) and fluid conductance (between 1.04 ×10−9 and 2.27 × 10−9 m4s/kg) of the β-TCP scaffolds tested, and the ability to finely tune the architecture using 3D printing, resulted in the development of novel bioink formulations and made available a versatile manufacturing process with broad applicability in producing substrates suitable for biomedical applications.

scholarly journals High strength and low swelling composite hydrogels from gelatin and delignified wood

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shennan Wang ◽  
Kai Li ◽  
Qi Zhou
Keyword(s):  
Mechanical Strength ◽  
High Strength ◽  
Honeycomb Structure ◽  
Composite Hydrogel ◽  
High Porosity ◽  
High Mechanical Strength ◽  
Composite Hydrogels ◽  
Freeze Dried ◽  
Strength And Stiffness ◽  
Phosphate Buffered Saline

Abstract A delignified wood template with hydrophilic characteristics and high porosity was obtained by removal of lignin. Gelatin was infiltrated into the delignified wood and further crosslinked with a natural crosslinker genipin to form hydrogels. The composite hydrogels showed high mechanical strength under compression and low swelling in physiological condition. The effect of genipin concentrations (1, 50 and 100 mM) on structure and properties of the composite hydrogels were studied. A porous honeycomb structure with tunable pore size and porosity was observed in the freeze-dried composite hydrogels. High elastic modulus of 11.82 ± 1.51 MPa and high compressive yield stress of 689.3 ± 34.9 kPa were achieved for the composite hydrogel with a water content as high as 81%. The equilibrium water uptake of the freeze-dried hydrogel in phosphate buffered saline at 37 °C was as low as 407.5%. These enables the delignified wood structure an excellent template in composite hydrogel preparation by using infiltration and in-situ synthesis, particularly when high mechanical strength and stiffness are desired.

scholarly journals Bovine Serum Albumin Adsorption onto Hydroxyapatite and Biphasic Calcium Phosphate Ceramic Granules

2016 ◽  
Vol 721 ◽  
pp. 197-201 ◽  
Author(s):  
Inga Jurgelane ◽  
Armands Buss ◽  
Dagnija Loca
Keyword(s):  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphate Ceramic ◽  
Beta Tricalcium Phosphate ◽  
Albumin Adsorption ◽  
Hydroxyapatite Formation

Calcium phosphate ceramics are one of the most studied biomaterials for bone substitution and regeneration. Bioactivity is one of the most important properties for these materials and it can be evaluated by adsorption of proteins and by hydroxyapatite formation on the surface in simulated body fluid (SBF). The aim of this work was to evaluate the bioactivity of ceramic granules with various hydroxyapatite (HAp) and beta tricalcium phosphate (β-TCP) ratios by adsorption of bovine serum albumin (BSA) in SBF and phosphate saline buffer (PBS) solution. The highest adsorption capacity in both solutions was observed for biphasic calcium phosphate sample with HAp/β-TCP ratio 50/50 but the lowest – for sample 10/90. The adsorption capacity of all samples was more than 2 times higher in SBF media than in PBS. The possibility of hydroxyapatite formation was evaluated by changes of Ca2+ concentration in SBF. After 5 days of incubation at 37oC all samples showed a decrease in Ca2+ concentration.

Strong and Bioactive Tri-Calcium Phosphate Scaffolds with Tube-Like Macropores

2014 ◽  
Vol 19 ◽  
pp. 65-75 ◽  
Author(s):  
Wei Zheng ◽  
Gang Liu ◽  
Cheng Yan ◽  
Yin Xiao ◽  
Xi Geng Miao
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
High Porosity ◽  
Calcium Phosphate Ceramic ◽  
Si Doped ◽  
Butadiene Styrene

Calcium phosphate ceramic scaffolds have been widely investigated for bone tissue engineering due to their excellent biocompatibility and biodegradation. Unfortunately, they have low mechanical properties, which inversely restrict their wide applications in load-bearing bone tissue engineering. In this study, porous Si-doped tri-calcium phosphate (TCP) ceramics with a high porosity (~65%) and with interconnected macrotubes (~0.8mm in diameter) and micropores (5-100 μm) were prepared by firing hydroxyapatite (HA)/ bioactive glass-impregnated acrylontrile butadiene styrene (ABS) templates at 1400 °C. Results indicated that the cylindrical scaffolds had a higher compressive strength than the cubic scaffolds and the smallest cylindrical scaffold had a highest compressive strength (14.68+0.2MPa). Additional studies of cell attachment and MTT cytotoxicity assay proved the bioactivity and biocompatibility of the Si-doped TCP scaffolds.

Strength of Calcium Phosphate Cements

2006 ◽  
Vol 975 ◽  
Author(s):  
Alexander Veresov ◽  
Alexander Stepuk ◽  
Alexander Kuznetsov ◽  
Valery Putlayev ◽  
Vladimir Kuznetsov
Keyword(s):  
Calcium Phosphate ◽  
Mechanical Strength ◽  
Calcium Phosphates ◽  
Main Idea ◽  
Bonded Materials ◽  
Calcium Phosphate Cements ◽  
Stiffness Characteristic ◽  
Calcium Phosphate Particles ◽  
Strength And Stiffness ◽  
Chitosan Biopolymer

ABSTRACTCalcium phosphates materials are widely used to treat the bones defects. HA bioceramics traditionally used in medicine has some serious disadvantage – week resorbility properties. Calcium phosphate cements seems to be promising compounds to replace conventional ceramics. High macroporosity of hardened materials leads to low mechanical strength. The main objective of present work was a development of new chemically bonded materials based on α-tricalcium phosphate (α-TCP) and hydroxylapatite phase. The main idea was to combine the benefit of ceramics technology (compacting of powders to reduce the starting macroporosity) to improve the materials strength with advantages of cement product (small resorbable calcium phosphate particles). α-Ca3(PO4)2-based biocements were synthesized with initial composition of HA, α- and β-TCP containing 80% wt. of TCP phase. The rate of α-TCP transformation to apatite phase during setting reaction was about 20% wt. in case of cylindrical samples (8mm × 8 mm) at 60°C for 50 hours. The fabricated TCP – bio-composites demonstrated high mechanical strength and stiffness characteristic. Most efficient results were achieved with chitosan biopolymer containing samples (σc = 120 MPa).

Preparation and Characterization of Modified Biphasic Calcium Phosphate Ceramics

2012 ◽  
Vol 506 ◽  
pp. 74-77 ◽  
Author(s):  
Naruporn Monmaturapoj ◽  
Witoon Thepsuwan
Keyword(s):  
Thermal Stability ◽  
Calcium Phosphate ◽  
Flexural Strength ◽  
Mechanical Strength ◽  
Bone Substitute ◽  
Biomedical Applications ◽  
Biphasic Calcium Phosphate ◽  
Bending Strength ◽  
Thermal Stability Of

Biphasic calcium phosphate (BCP) ceramic is commonly used in the biomedical applications particularly as a bone substitute due to its biocompatibility and directly bond to bones. However, the mechanical strength is quite poor. Therefore, well known biocompatible and strong ceramics such as SiO2, ZrO2 and TiO2 were added to improve the strength of BCP. BCP powder with HA/TCP ratios of 70/30 (HAP7030) was obtained by controlling the calcining temperature of the mixture between a pure HA and TCP. SiO2, ZrO2 and TiO2 powder with 2, 5 and 10 %wt were mixed with the HAP7030 powder by ball milling in ethanol. The mixtures were dried, pressed and sintered at 1100°C for 2 hrs. XRD and SEM were used to determine crystal structures and morphology of the sintered samples, respectively. Physical properties and flexural strength of samples were measured. Results showed that the bending strength of HAP7030 sample was rather improved by adding TiO2 than the addition of SiO2 or ZrO2. With increasing TiO2, HAP7030 strength was superior and HAP7030 with 10 %wt of TiO2 obtained the optimum bending strength around 61 MPa. However, the addition of TiO2 induced the thermal stability of HA/TCP, in which HA completely decomposed to β-TCP in this study.

Direct 3D Printing of Seashell Precursor toward Engineering a Multiphasic Calcium Phosphate Bone Graft

2021 ◽  
Author(s):  
Prabhash Dadhich ◽  
Pavan Kumar Srivas ◽  
Bodhisatwa Das ◽  
Pallabi Pal ◽  
Joy Dutta ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
3D Printing ◽  
Bone Graft

Chitosan hydrogels in 3D printing for biomedical applications

2021 ◽  
Vol 260 ◽  
pp. 117768
Author(s):  
Mina Rajabi ◽  
Michelle McConnell ◽  
Jaydee Cabral ◽  
M. Azam Ali
Keyword(s):  
3D Printing ◽  
Biomedical Applications ◽  
Chitosan Hydrogels

3D printing biomimetic materials and structures for biomedical applications

2021 ◽  
Author(s):  
Yizhen Zhu ◽  
Dylan Joralmon ◽  
Weitong Shan ◽  
Yiyu Chen ◽  
Jiahui Rong ◽  
...  
Keyword(s):  
3D Printing ◽  
Biomedical Applications ◽  
Biomimetic Materials

scholarly journals Comparative Study of Silk Fibroin-Based Hydrogels and Their Potential as Material for 3-Dimensional (3D) Printing

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3887
Author(s):  
Watcharapong Pudkon ◽  
Chavee Laomeephol ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont ◽  
Juthamas Ratanavaraporn
Keyword(s):  
3D Printing ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Structure Stability ◽  
3 Dimensional ◽  
Critical Technology ◽  
Box Structure ◽  
High Degree

Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1–3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.

scholarly journals Microstructured Surface Plasmon Resonance Sensor Based on Inkjet 3D Printing Using Photocurable Resins with Tailored Refractive Index

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2518
Author(s):  
Nunzio Cennamo ◽  
Lorena Saitta ◽  
Claudio Tosto ◽  
Francesco Arcadio ◽  
Luigi Zeni ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
3D Printing ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Optical Sensor ◽  
Low Cost ◽  
3D Print ◽  
Spr Sensor ◽  
Resonance Sensor ◽  
3D Printed

In this work, a novel approach to realize a plasmonic sensor is presented. The proposed optical sensor device is designed, manufactured, and experimentally tested. Two photo-curable resins are used to 3D print a surface plasmon resonance (SPR) sensor. Both numerical and experimental analyses are presented in the paper. The numerical and experimental results confirm that the 3D printed SPR sensor presents performances, in term of figure of merit (FOM), very similar to other SPR sensors made using plastic optical fibers (POFs). For the 3D printed sensor, the measured FOM is 13.6 versus 13.4 for the SPR-POF configuration. The cost analysis shows that the 3D printed SPR sensor can be manufactured at low cost (∼15 €) that is competitive with traditional sensors. The approach presented here allows to realize an innovative SPR sensor showing low-cost, 3D-printing manufacturing free design and the feasibility to be integrated with other optical devices on the same plastic planar support, thus opening undisclosed future for the optical sensor systems.

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