scholarly journals Identification and Bioactivities of Secondary Metabolites Derived from Endophytic Fungi Isolated from Ethnomedicinal Plants of Tujia in Hubei Province: A Review

2021 ◽  
Author(s):  
Ke Ye ◽  
Hong-Lian Ai ◽  
Ji-Kai Liu
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Aromatic Compounds ◽  
Heterocyclic Compounds ◽  
New Drugs ◽  
National Minority ◽  
Natural Plant ◽  
Aliphatic Compounds ◽  
Bioactive Natural Products

AbstractTujia is a national minority, inhabiting in the mountainous Wuling area in China. Since 1978, Tujia medicine has been studied, summarized and developed, leading to numerous achievements by Chinese researchers, such as the publishing of approximately 30 monographs of Tujia medicine. These publications are focused on summarizing and improving the theory of Tujia medicine and developing clinical therapies from this system of medicine. The shortage of natural medicinal plants used in Tujia medicine has created the need to discover new resources to replace them and protect endangered natural plant species. Endophytic fungi are one of the conservation options, are considered a source of new bioactive natural products, and are a renewable and inexhaustible source of new drugs and agrochemicals. This review summarizes 260 compounds from endophytic fungi that have been previously isolated from the medicinal plants of Tujia. These compounds include steroids, terpenoids, meroterpenoids, polyketides, alkaloids, peptides, aliphatic compounds, aromatic compounds, and heterocyclic compounds. Graphic Abstract

scholarly journals New and bioactive natural products from an endophyte of Panax notoginseng

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 38100-38109 ◽  
Author(s):  
Jun Xie ◽  
Ying-Ying Wu ◽  
Tian-Yuan Zhang ◽  
Meng-Yue Zhang ◽  
Wei-Wei Zhu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Antiviral Activity ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Panax Notoginseng ◽  
Bioactive Natural Products

Secondary metabolites with cytotoxic activity, antiviral activity and antimicrobial activity from the endophytic fungi of Panax notoginseng.

scholarly journals Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

2021 ◽  
Vol 12 ◽  
Author(s):  
Jucélia Iantas ◽  
Daiani Cristina Savi ◽  
Renata da Silva Schibelbein ◽  
Sandriele Aparecida Noriler ◽  
Beatriz Marques Assad ◽  
...  
Keyword(s):  
New Species ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Dna Sequences ◽  
Mycelial Growth ◽  
High Performance ◽  
Biological Activities ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Bioactive Natural Products

Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

scholarly journals Endophytic fungal isolation from Blumea axillaris: Identification and biological activity of secondary metabolites

2021 ◽  
Vol 13 (2) ◽  
pp. 10953
Author(s):  
Nehru LAVANYA ◽  
Vellingiri MANON MANI ◽  
Nachimuthu SARANYA ◽  
Rajendran DEEPAKKUMAR ◽  
Kathirvel PREETHI
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Dna Sequences ◽  
Radical Scavenging ◽  
Antagonistic Activity ◽  
Diffusion Method ◽  
Fungal Isolation ◽  
Sordaria Fimicola ◽  
Phomopsis Phaseoli

Medicinal plants are a wealthy source of natural medicinal properties and remain as base for new drug discoveries. Endophyte from the specific medicinal plants produce the analogous metabolites as that of the host plant. The metabolites from the endophytes comprise maximum therapeutic properties and have been extensively applied in treating various diseases and disorders. This study was focused on identification of the endophytic fungi from the medicinal plant Blumea axillaris and investigates the diversity of endophytic fungi from various explants of the same plant. The explants were cultured on potato dextrose agar and 6 endophytic fungi were successfully isolated from Blumea axillaris. They were identified morphologically and confirmed with molecular analysis as Xylaria arbuscula, Paraphoma radicina, Phomopsis phaseoli, Sordaria fimicola, Aspergillus amstelodami, Diaporthe eucalyptorum. The DNA sequences were analyzed by BLAST and the phylogenetic tree was constructed with neighbor joining method. The six isolates were subjected to antagonistic activity for the selection of potential strain and the bioactive strain Xylaria arbuscula was selected for the production of secondary metabolites by optimization. The parameters like pH, temperature, incubation period, carbon and nitrogen (organic and inorganic source) were optimized for secondary metabolite production. The fungal metabolite was extracted by solvent extraction method using polar and non-polar solvents like propanol, methanol, chloroform, acetone and ethyl acetate. To investigate the bioactivities of the fungal crude extract was subjected first for its antioxidant activity using DPPH radical scavenging method, followed by antimicrobial activity of methanolic (MeOH) extract of Xylaria arbuscula, that were also analyzed by the agar well-diffusion method against the clinical pathogens Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae, Proteus mirabilis, Aspergillus niger and Candida albicans.

scholarly journals Secondary Metabolite and Antioxidant Activity of Endophytic Fungi Isolated from Syzygium aqueum Leaves Stalk

2021 ◽  
Vol 12 (6) ◽  
pp. 7584-7595
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Crude Extract ◽  
Pure Compound ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Surface Sterilization

Medicinal plants are a promising host for endophytic fungi to produce secondary metabolites relevant for food and health. In this study, we evaluate antioxidant activity to determine the species of endophytic fungi isolated from Syzygium aqueum. Endophytic fungi were isolated from leaf stalks through surface sterilization. The fungi’s isolate was identified with morphology and molecular analysis (ITS-rDNA). The pure fungi strain was cultivated on PDB media for 4 weeks, and metabolites were extracted using ethyl acetate. The crude extract of endophytic fungi was examined for its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The pure compound was isolated using the chromatography method, and its structure was determined using spectroscopy analysis involving NMR 1D and 2D. In total, four obtained endophytic fungi were isolated from leaf stalks. The fungi with good antioxidant activity (IC50 59.2 µg/mL) were identified as Beltrania rhombica. The characteristics of the pure compound are white-yellowish powder with IC50 44.2 µg/mL. Based on spectroscopy analysis, the pure compound was identified as 3-(hydroxyl(3,4,5-trihydroxylphenyl)methyl)-3,4-dihydro-2H-pyran-4,5,6-triol.

scholarly journals PERANAN MIKROBA ENDOFIT UNTUK PENGEMBANGAN OBAT ANTI KANKER

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Siti Rofida
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Anticancer Activity ◽  
Natural Product ◽  
Host Plant ◽  
Anticancer Drugs ◽  
Endophytic Fungi

Natural product that has developed as anticancer drugs are consists from secondary metabolites that has isolated from plant, ocean plant and microorganisms. To maintain its productivity, must be concerned to the cultivation of the medicinal plants. Medicinal plants need time to grow and producing secondary metabolites that would be isolated, so that the production of secondary metabolites compound from plant is very limited.             The plants that infected by endophytic fungi be able to grow faster than the normal plants. Endophytic fungi also have ability producing secondary metabolites compound that produced by the host plant. Taxol, podophyllotoxin and camptothecin are compounds that proved has anticancer activity. Endophytic fungi have also proved increasing the production of the compound.

scholarly journals Secrets of plants: Endophytes

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Asiya Nazir ◽  
Habeeb Abdul Rahman
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Endophytic Fungi ◽  
Potential Candidate ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Bioactive Secondary Metabolites ◽  
Novel Drug ◽  
The Impact

Endophytic fungi are an important component that colonizes in healthy tissues of living plants and can be readily isolated from any microbial or plant growth medium. They act as reservoirs of novel bioactive secondary metabolites, such as alkaloids, phenolic acids, quinones, steroids, saponins, tannins, and terpenoids that serve as a potential candidate for antimicrobial, anti-insect, anticancer and many more properties. Their huge diversity and particular habituation, they can provide a good area for research in the field of making new medicines and novel drug-like molecules. Because of the impact of endophytes on host plant by enhancing their growth or increasing their fitness, also making them tolerant to abiotic and biotic stresses and holding the secondary metabolites, endophytes are gaining attention as a subject for research. This review aims to comprehend the contribution and uses of endophytes and relationships between endophytic fungi and their host medicinal plants.

Bioactive Metabolites from Pathogenic and Endophytic Fungi of Forest Trees

2018 ◽  
Vol 25 (2) ◽  
pp. 208-252 ◽  
Author(s):  
Marco Masi ◽  
Lucia Maddau ◽  
Benedetto Teodoro Linaldeddu ◽  
Bruno Scanu ◽  
Antonio Evidente ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Terrestrial Ecosystems ◽  
Biological Properties ◽  
Research Literature ◽  
New Drugs ◽  
Bioactive Metabolites ◽  
Bibliographic Databases ◽  
Forest Trees

Background: Fungi play an important role in terrestrial ecosystems interacting positively or negatively with plants. These interactions are complex and the outcomes are different depending on the fungal lifestyles, saprotrophic, mutualistic or pathogenic. Furthermore, fungi are well known for producing secondary metabolites, originating from different biosynthetic pathways, which possess biological properties of considerable biotechnological interest. Among the terrestrial ecosystems, temperate forests represent an enormous reservoir of fungal diversity. This review will highlight the goldmine of secondary metabolites produced by pathogenic and endophytic fungi of forest trees with focus on their biological activities. Methods: A structured search of bibliographic databases for peer-reviewed research literature was undertaken using a research discovery application providing access to a large and authoritative source of references. The papers selected were examined and the main results were reported and discussed. Results: Two hundred forthy-one papers were included in the review, outlined a large number of secondary metabolites produced by pathogenic and endophiltic fungi and their biological activities, including phytotoxic, antifungal, antioomycetes, antibacterial, brine shrimp lethality, mosquito biting deterrence and larvicidal, cytotoxic, antiproliferative and many other bioactivities. Conclusion: The findings of this review confirm the importance of secondary metabolites produced by pathogenic and endophytic fungi from forest plants growing in temperate regions as an excellent prospects to discover compounds with new bioactivities and mode of actions. In addition, the potential of some metabolites as a source of new drugs and biopesticides is underlined.

scholarly journals Effect of water deficits on the growth and secondary metabolites of Salvia dolomitica Codd. and Salvia namaensis Schinz

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Shaheed Roos ◽  
Felix Nchu
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Water Deficit ◽  
Water Deficits ◽  
Bioactive Natural Products ◽  
Fungal Activity ◽  
University Of Technology ◽  
Cape Peninsula ◽  
Number Of Stems ◽  
Anti Fungal

Background: Salvia dolomitica Codd. and Salvia namaensis Schinz. are indigenous to southern Africa and are used as medicinal plants in folk medicine.Aim: This study aimed to assess the effects of different levels of water deficit treatments on the growth, concentration of secondary metabolites, and anti-Fusarium oxysporum activity of S. dolomitica and S. namaensis.Setting: Experiments were carried out on the Bellville campus of the Cape Peninsula University of Technology, Cape Town.Methods: Four weeks old seedlings of the two species were subjected to 3-, 6-, 9- and 12-day water deficit regimes. Secondary metabolites such as polyphenols, alkaloids, and flavanols were assessed using spectroscopic methods. The anti-fungal activities of crude extracts obtained from plants were evaluated in a micro-dilution bioassay.Results: In all treatments, the plant height, crown width, number of stems and leaves, and fresh and dry weights reduced with increased water deficits. Acetone extracts from all treatments showed anti-fungal activity. However, extracts from the treatment with moderate water deficit (6-day watering interval) recorded significantly (P 0.01) better inhibition of F. oxysporum at the 18 h post incubation than the commercial fungicide, Mancozeb.Conclusion: This research has revealed that mild to moderate water deficit level favours the accumulation of alkaloids in S. dolomitica. Meanwhile, mild to severe water deficit significantly lowered flavanol content in S. namaensis. There was a correlation between the increase in total alkaloid contents and the enhanced anti-fungal activity of extracts of S. dolomitica. The present findings pave the way for optimised cultivation of medicinal plants and development of bioactive natural products.

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