Assessment of wound healing efficacy of Growth Factor Concentrate (GFC) in non-diabetic and diabetic Sprague Dawley rats

2021 ◽  
Author(s):  
Manohar Nandanwar ◽  
Vijay Sharma ◽  
Avinash Karade ◽  
Anuka Sharma ◽  
Atul Kansagara ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Factor Concentrate

Oral administration of Aloe vera ameliorates wound healing through improved angiogenesis and chemotaxis in Sprague Dawley rats

2020 ◽  
Vol 21 ◽  
Author(s):  
Fatima Ali ◽  
Nadia Wajid ◽  
Maryam Ghulam Sarwar ◽  
Aamer Mahmood Qazi
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Growth Factor ◽  
Oral Administration ◽  
Aloe Vera ◽  
Collagen Content ◽  
Pcr Analysis ◽  
Sprague Dawley ◽  
Rt Pcr ◽  
Sprague Dawley Rats

Background:: Aloe vera has been reported as a topical antibiotic and healing agent for wounds but advantages of oral administration and mechanisms of wound healing have not been reported. Present study focuses on evaluation of effects of oral administration of Aloe vera for excisional cutaneous wounds in Sprague Dawley rats. Methods:: Sprague Dawley (SD) rats were inflicted with excisional wounds and then, either treated with Aloe vera orally (Aloe vera) or kept untreated (wound) while healthy rats were kept as control group. Wound area was measured from seven day to 21st day. Collagen content was estimated by hydroxyproline assay. Histology was analysed by hematoxylin and eosin staining. Angiogenesis was observed by indirect ELISA for Insulin like Growth Factor (IGF-1) and Vascular Endothelial Growth Factor (VEGF) protein from skin, serum and bone marrow. Chemotaxis was evaluated by RT-PCR analysis for Stromal cell-derived factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) from skin and bone marrow. Results:: Aloe vera healed wounds earlier than untreated rats with gradual improvement in wound areas and collagen content. Aloe vera also improved the expression of IGF-1 and VEGF in skin and bone marrow indicating improvement in angiogenesis. RT- PCR analysis showed increased expression of genes for chemotaxis (SDF-1 and CXCR-4), in skin and bone marrow.

scholarly journals Effectiveness Of Stepping Dose Ozonated Virgin Coconut Oil For Wound Healing In Autologous Full Thickness Skin Graft In Terms Of Fibroblast Proliferation And The Expression Of Vascular Endothelial Growth Factor In Sprague Dawley Rats

2021 ◽  
Vol 8 (1) ◽  
pp. 28-38
Author(s):  
Johan Rinto Even Napitupulu ◽  
Hardian Hardian ◽  
Najatullah Najatullah
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Skin Graft ◽  
Coconut Oil ◽  
Vascular Endothelial ◽  
Sprague Dawley ◽  
Virgin Coconut Oil ◽  
Sprague Dawley Rats ◽  
Endothelial Growth Factor

Abstract Background: Now, Skin grafts are one of the therapies of choice in the wound healing process. And this is still developing today. The process of formation fibroblas and the formation of vascular endothelial growth factor have an important role in healing skin graft wounds. Ozonated virgin coconut oil has an important role in wound healing. Objective: To prove the effect of ozonated virgin coconut oil in various doses in increasing number of fibroblas and expression of vascular endothelial growth factor of skin graft wounds. Methods: This study is an experimental study with "Randomized parallel study with controlled group design" on 40 Sprague Dawley rats which are performed an autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 and K2 = without ozonated VCO), (A1 and A2 = ozonated VCO 50,4 mg/ml), (B1 and B2 = ozonated VCO  103,2 mg/ml), (C1 and C2  = ozonated VCO 204  mg/ml). Evaluation of the amount of fibroblas was done by staining hematoxylin & eosin after the 6th day and the expression of vascular endothelial growth factor  was done by staining immunohistochemistry after the 12th  day after skin graft. Results: Statistical analysis of the amount of fibroblas after the 6th  day was found to be significantly different between the K1 vs A1 group (p = 0.029), K1 vs B1 (p = 0.004), K1 vs C1 (p = 0.000), on the 12th day was found significantly different the amount of fibroblast between K2 vs A2 ( p = 0,029 ), K2 vs B2 ( p = 0,010 ), K2 vs C2 ( p = 0,001 ). The expression of vascular endothelial growth factor on the 6th day was found a significant difference between the K1 vs A1 group (p = 0.024), K1 vs B1 (p = 0.005), K1 vs C1 (p = 0.001), on the 12th day was found significantly different the expression of vascular endothelial growth factor between K2 vs A2 ( p = 0,011 ), K2 vs B2 ( p = 0,036 ), K2 vs C2 ( p = 0,004 ) . Conclusion: Ozonated VCO can increase the amount of fibroblas and expression of vascular endothelial growth factor  in the wound healing process of autologous skin graft in Sprague Dawley rats. Keywords: Ozonated VCO, full thickness skin graft, fibroblas, VEGF.

scholarly journals N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

2021 ◽  
Vol 22 (14) ◽  
pp. 7549
Author(s):  
Wiktor Paskal ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
Albert Stachura ◽  
Kacper Pełka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Time Points ◽  
Model Study ◽  
Sprague Dawley Rats ◽  
Animal Model Study ◽  
Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

scholarly journals Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

2021 ◽  
Vol 33 (2) ◽  
pp. 145
Author(s):  
Hendry Rusdy ◽  
Astri Suryani Pasaribu Saruksuk ◽  
Rahmi Syaflida Dalimunte ◽  
Gostry Aldica Dohude
Keyword(s):  
Wound Healing ◽  
Tranexamic Acid ◽  
Tooth Extraction ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Jatropha Multifida ◽  
Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine 

scholarly journals Angelica dahurica and Rheum officinale Facilitated Diabetic Wound Healing by Elevating Vascular Endothelial Growth Factor

2021 ◽  
pp. 1-19
Author(s):  
Yuh-Huey Chao ◽  
Wan-Ting Yang ◽  
Ming-Chang Li ◽  
Fwu-Lin Yang ◽  
Ru-Ping Lee
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Smooth Muscle Actin ◽  
Intraperitoneal Administration ◽  
Angelica Dahurica ◽  
Vascular Endothelial ◽  
Sprague Dawley ◽  
Diabetic Wounds ◽  
Endothelial Growth Factor

Traditional Chinese medicine (TCM) provides alternative treatment choices for diabetic wounds. The aim of this study was to evaluate the effects of Angelica dahurica and Rheum officinale (ARE) on diabetic wounds and its underlying action mechanism. A total of 36 healthy male Sprague–Dawley rats were randomly divided into three groups: diabetes mellitus (DM) rats treated with ARE (DM-ARE), DM rats treated with 0.9% saline (DM-NS), and non-DM rats treated with 0.9% saline (NDM-NS). DM was induced by intraperitoneal administration of 40 mg/kg of streptozotocin after a 2-week high-fat diet feeding. After excisional skin wounds and treatments, the remaining wound area (RWA) in each group was measured. The RWA in the DM-NS group (69.60% ± 2.35%) was greater than that in the DM-ARE (55.70% ± 1.85%) and NDM-NS groups (52.50% ± 2.77%) on day 6. Besides, the DM-ARE group showed higher vascular endothelial growth factor (VEGF), higher inducible nitric oxide synthase (iNOs), higher [Formula: see text]-smooth muscle actin ([Formula: see text]-SMA), and lower nuclear factor kappa-light-chain-enhancer of activated B cell (NF-[Formula: see text]B) expression in the wound skin tissue. These results showed that treatment with ARE shifted the recovery pattern of diabetic rats to the pattern of nondiabetic rats, indicating that ARE may improve wound healing in diabetic conditions.

scholarly journals Expression of nerve growth factor messenger RNA in pancreatic cancer tissues in Sprague Dawley rats

2005 ◽  
Vol 13 (18) ◽  
pp. 2227
Author(s):  
Zhu-Lin Yang ◽  
Xing-Hui Deng ◽  
Le-Ping Yang ◽  
Qing-Long Li ◽  
Wen-Tao Fan ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Messenger Rna ◽  
Sprague Dawley ◽  
Nerve Growth ◽  
Sprague Dawley Rats ◽  
Cancer Tissues

Epidermal growth factor accelerates renal tissue repair in a model of gentamicin nephrotoxicity in rats

1992 ◽  
Vol 263 (5) ◽  
pp. F806-F811 ◽  
Author(s):  
N. J. Morin ◽  
G. Laurent ◽  
D. Nonclercq ◽  
G. Toubeau ◽  
J. A. Heuson-Stiennon ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Sites ◽  
Renal Tissue ◽  
Sprague Dawley ◽  
Renal Tubular Cells ◽  
Sprague Dawley Rats ◽  
Epidermal Growth ◽  
Renal Tubular ◽  
Cessation Of Treatment

Epidermal growth factor (EGF) is a potent mitogen for renal tubular cells that possess specific high-affinity binding sites for this polypeptide. However, actual function of EGF within the kidney remains to be elucidated. We evaluated the effect of exogenous EGF administration on the rate of tubular regeneration in an experimental model of gentamicin (GT) nephrotoxicity. Female Sprague-Dawley rats were anesthetized, and a miniosmotic pump filled with mouse EGF or saline was implanted subcutaneously. Twenty-four hours later, GT (40 mg.kg-1 x 12 h-1 ip) was given for 4 and 8 days. Groups of treated animals and controls were killed either the day after cessation of treatment (days 5 and 9) or 4 and 8 days after the end of 8-day GT administration (days 12 and 16). Cortical GT levels of groups killed at days 5, 9, 12, and 16 were similar in animals infused with saline or EGF. Serum creatinine levels were significantly higher in GT-treated animals infused with EGF or saline and killed at days 9 and 12 compared with saline-treated animals infused with EGF or saline alone (P < 0.01). Blood urea nitrogen (BUN) also increased as a result of GT administration. However, in animals receiving GT and EGF and killed at day 16, mean BUN level was significantly lower (P < 0.01) compared with rats dosed with GT alone. In treated rats, the extent of tubular regeneration, evaluated by the rate of [3H]thymidine incorporation into renal cortical DNA or by the frequency of S-phase cells (histoautoradiography), was increased in a dose- and time-dependent fashion.(ABSTRACT TRUNCATED AT 250 WORDS)

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