scholarly journals Role of Non-coding RNAs in Fungal Pathogenesis and Antifungal Drug Responses

2020 ◽  
Vol 7 (4) ◽  
pp. 133-141 ◽  
Author(s):  
Sourabh Dhingra
Keyword(s):  
Gene Expression ◽  
Small Rnas ◽  
Fungal Pathogens ◽  
Antifungal Drugs ◽  
Antifungal Drug ◽  
Fungal Pathogenesis ◽  
Regulate Gene Expression ◽  
Drug Responses ◽  
Non Coding Rnas ◽  
Regulate Gene

Abstract Purpose of Review Non-coding RNAs (ncRNAs), including regulatory small RNAs (sRNAs) and long non-coding RNAs (lncRNAs), constitute a significant part of eukaryotic genomes; however, their roles in fungi are just starting to emerge. ncRNAs have been shown to regulate gene expression in response to varying environmental conditions (like stress) and response to chemicals, including antifungal drugs. In this review, I highlighted recent studies focusing on the functional roles of ncRNAs in pathogenic fungi. Recent Findings Emerging evidence suggests sRNAs (small RNAs) and lncRNAs (long non-coding RNAs) play an important role in fungal pathogenesis and antifungal drug response. Their roles include posttranscriptional gene silencing, histone modification, and chromatin remodeling. Fungal pathogens utilize RNA interference (RNAi) mechanisms to regulate pathogenesis-related genes and can also transfer sRNAs inside the host to suppress host immunity genes to increase virulence. Hosts can also transfer sRNAs to induce RNAi in fungal pathogens to reduce virulence. Additionally, sRNAs and lncRNAs also regulate gene expression in response to antifungal drugs increasing resistance (and possibly tolerance) to drugs. Summary Herein, I discuss what is known about ncRNAs in fungal pathogenesis and antifungal drug responses. Advancements in genomic technologies will help identify the ncRNA repertoire in fungal pathogens, and functional studies will elucidate their mechanisms. This will advance our understanding of host-fungal interactions and potentially help develop better treatment strategies.

scholarly journals Small RNAs with 5′-Polyphosphate Termini Associate with a Piwi-Related Protein and Regulate Gene Expression in the Single-Celled Eukaryote Entamoeba histolytica

2008 ◽  
Vol 4 (11) ◽  
pp. e1000219 ◽  
Author(s):  
Hanbang Zhang ◽  
Gretchen M. Ehrenkaufer ◽  
Justine M. Pompey ◽  
Jason A. Hackney ◽  
Upinder Singh
Keyword(s):  
Gene Expression ◽  
Entamoeba Histolytica ◽  
Small Rnas ◽  
Related Protein ◽  
Regulate Gene Expression ◽  
Regulate Gene

scholarly journals Dietary microRNA—A Novel Functional Component of Food

2019 ◽  
Vol 10 (4) ◽  
pp. 711-721 ◽  
Author(s):  
Lin Zhang ◽  
Ting Chen ◽  
Yulong Yin ◽  
Chen-Yu Zhang ◽  
Yong-Liang Zhang
Keyword(s):  
Gene Expression ◽  
Small Rnas ◽  
Biological Significance ◽  
Circulatory System ◽  
Physiological Effects ◽  
Biological Processes ◽  
Regulate Gene Expression ◽  
Functional Component ◽  
Health And Disease ◽  
Regulate Gene

ABSTRACT MicroRNAs are a class of small RNAs that play essential roles in various biological processes by silencing genes. Evidence emerging in recent years suggests that microRNAs in food can be absorbed into the circulatory system and organs of humans and other animals, where they regulate gene expression and biological processes. These food-derived dietary microRNAs may serve as a novel functional component of food, a role that has been neglected to date. However, a significant amount of evidence challenges this new concept. The absorption, stability, and physiological effects of dietary microRNA in recipients, especially in mammals, are currently under heavy debate. In this review, we summarize our current understanding of the unique characteristics of dietary microRNAs and concerns about both the mechanistic and methodological basis for studying the biological significance of dietary microRNAs. Such efforts will benefit continuing investigations and offer new perspectives for the interpretation of the roles of dietary microRNA with respect to the health and disease of humans and animals.

microRNAs: a new frontier in kallikrein research

2008 ◽  
Vol 389 (6) ◽  
Author(s):  
George M. Yousef
Keyword(s):  
Gene Expression ◽  
Human Tissue ◽  
In Silico ◽  
Regulatory Mechanism ◽  
Transcriptional Level ◽  
Regulate Gene Expression ◽  
New Frontier ◽  
Experimental Approaches ◽  
Non Coding Rnas ◽  
Regulate Gene

Abstract microRNAs (miRNAs) are a recently discovered class of small non-coding RNAs that regulate gene expression. Rapidly accumulating evidence has revealed that miRNAs are associated with cancer. The human tissue kalli-krein gene family is the largest contiguous family of proteases in the human genome, containing 15 genes. Many kallikreins have been reported as potential tumor markers. In this review, recent bioinformatics and experimental evidence is presented indicating that kallikreins are potential miRNA targets. The available experimental approaches to investigate these interactions and the potential diagnostic and therapeutic applications are also discussed. miRNAs represent a possible regulatory mechanism for controlling kallikrein expression at the post-transcriptional level. Many miRNAs were predicted to target kallikreins and a single miRNA can target more than one kallikrein. Recent evidence suggests that miRNAs can also exert ‘quantitative’ control of kallikreins by utilizing multiple targeting sites in the kallikrein mRNA. More research is needed to experimentally verify the in silico predictions and to investigate the possible role in tumor initiation and/or progression.

scholarly journals MiR-147: Functions and Implications in Inflammation and Diseases

MicroRNA ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Ling Lin ◽  
Kebin Hu
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Neurodegenerative Disorder ◽  
Mrna Translation ◽  
Transcriptional Level ◽  
Regulate Gene Expression ◽  
Inflammatory Bowel ◽  
Non Coding Rnas ◽  
Regulate Gene

: MicroRNAs (miRNAs) are small non-coding RNAs (19~25 nucleotides) that regulate gene expression at a post-transcriptional level through repression of mRNA translation or mRNA decay. miR-147, which was initially discovered in mouse spleen and macrophages, has been shown to correlate with coronary atherogenesis and inflammatory bowel disease and modulate macrophage functions and inflammation through TLR-4. The altered miR-147 level has been shown in various human diseases, including infectious disease, cancer, cardiovascular disease, a neurodegenerative disorder, etc. This review will focus on the current understanding regarding the role of miR-147 in inflammation and diseases.

How do microRNAs regulate gene expression?

2008 ◽  
Vol 36 (6) ◽  
pp. 1224-1231 ◽  
Author(s):  
Ian G. Cannell ◽  
Yi Wen Kong ◽  
Martin Bushell
Keyword(s):  
Gene Expression ◽  
Untranslated Region ◽  
Protein Production ◽  
Translational Repression ◽  
Regulate Gene Expression ◽  
Target Mrna ◽  
Target Mrnas ◽  
Non Coding Rnas ◽  
Regulate Gene

miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3′-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing. The exact mechanism of miRNA-mediated translational repression is yet to be fully determined, but recent data from our laboratory have shown that the stage of translation which is inhibited by miRNAs is dependent upon the promoter used for transcribing the target mRNA. This review focuses on understanding how miRNA repression is operating in light of these findings and the questions that still remain.

scholarly journals MicroRNAs in veterinary cardiology

2017 ◽  
Vol 47 (7) ◽  
Author(s):  
Marcela Wolf ◽  
Eloísa Muehlbauer ◽  
Marlos Gonçalves Sousa
Keyword(s):  
Gene Expression ◽  
Heart Diseases ◽  
Scientific Evidence ◽  
Cardiac Diseases ◽  
Regulate Gene Expression ◽  
Over Expression ◽  
Non Invasive ◽  
Therapeutic Value ◽  
Non Coding Rnas ◽  
Regulate Gene

ABSTRACT: The use of biomarkers is an important recent development in veterinary medicine. Biomarkers allow non-invasive quantification of substances with diagnostic and prognostic potential in several diseases. The microRNAs are small, non-coding RNAs that regulate gene expression and are expressed in different forms in many diseases. Reduced or over-expression of microRNAs showed to be part of the pathogenesis of some heart diseases in humans and animals. Diagnostic and therapeutic value of measuring microRNAs in veterinary cardiology is increased because abnormal expression can be managed by the use of antagonists (in the case of overexpression) and mimicking (in the case of underexpression). Thus, this literature review aimed to compile scientific evidence of dysregulation of microRNAs expression in different cardiac diseases being one of the promises in the therapeutic field and diagnosis of veterinary cardiology. MicroRNAs not only have potential as a biomarker but may also help in elucidation of aspects of the pathogenesis of a variety of diseases.

Epigenetherapy, a new concept

2011 ◽  
Vol 2 (3) ◽  
pp. 127-134
Author(s):  
Tiia Husso ◽  
Mikko P. Turunen ◽  
Nigel Parker ◽  
Seppo Ylä-Herttuala
Keyword(s):  
Gene Expression ◽  
Small Rnas ◽  
Basic Research ◽  
Clinical Applications ◽  
Regulate Gene Expression ◽  
Endogenous Gene ◽  
Rna Molecules ◽  
Regulate Gene

AbstractSmall RNAs have been shown to regulate gene transcription by interacting with the promoter region and modifying the histone code. The exact mechanism of function is still unclear but the feasibility to activate or repress endogenous gene expression with small RNA molecules has already been demonstrated in vitro and in vivo. In traditional gene therapy non-mutated or otherwise useful genes are inserted into patient's cells to treat a disease. In epigenetherapy the action of small RNAs is utilized by delivering only the small RNAs to patient's cells where they then regulate gene expression by epigenetic mechanisms. This method could be widely useful not only for basic research but also for clinical applications of small RNAs.

scholarly journals Interaction Between LncRNA and UPF1 in Tumors

2021 ◽  
Vol 12 ◽  
Author(s):  
Junjian He ◽  
Xiaoxin Ma
Keyword(s):  
Gene Expression ◽  
Rna Binding ◽  
Cell Transformation ◽  
Rna Binding Protein ◽  
Rna Decay ◽  
Regulate Gene Expression ◽  
Rna Molecules ◽  
Key Factor ◽  
Non Coding Rnas ◽  
Regulate Gene

Long non-coding RNAs (LncRNAs) can bind to other proteins or RNAs to regulate gene expression, and its role in tumors has been extensively studied. A common RNA binding protein, UPF1, is also a key factor in a variety of RNA decay pathways. RNA decay pathways serve to control levels of particular RNA molecules. The expression of UPF1 is often dysregulated in tumors, an observation which suggests that UPF1 contributes to development of a variety of tumors. Herein, we review evidence from studies of fourteen lncRNAs interact with UPF1. The interaction between lncRNA and UPFI provide fundamental basis for cell transformation and tumorigenic growth.

scholarly journals Re-arrangements in the cytoplasmic distribution of small RNAs following the maternal-to-zygotic transition inDrosophilaembryos

2017 ◽  
Author(s):  
Mehmet Ilyas Cosacak ◽  
Hatice Yiğit ◽  
Bünyamin Akgül
Keyword(s):  
Gene Expression ◽  
Early Development ◽  
Small Rnas ◽  
Complex Interplay ◽  
Sedimentation Pattern ◽  
Regulate Gene Expression ◽  
Translational Machinery ◽  
Maternal To Zygotic Transition ◽  
Cellular Translation ◽  
Regulate Gene

ABSTRACTSmall RNAs are known to regulate gene expression during early development. However, the dynamics of interaction between small RNAs and polysomes during this process is largely unknown. 0-1h and 7-8hDrosophilaembryos were fractionated on sucrose density gradients into four fractions based on A254reading (1) translationally inactive messengerribonucleoprotein (mRNP); (2) 60S; (3) monosome; and (4) polysome. Comparative analysis of deep-sequencing reads from fractionated and un-fractionated 0-1h and 8-h embryos revealed development-specific co-sedimentation pattern of small RNAs with the cellular translation machinery. Although most miRNAs did not have a specific preference for any state of the translational machinery, we detected fraction-specific enrichment of some miRNAs such as miR-1-3p, -184-39, 5-5p and 263-5p. More interestingly, we observed dysregulation of a subset of miRNAs in fractionated embryos despite no measurable difference in their amount in unfractionated embryos. Transposon-derived endosiRNAs are over-expressed in 7-8h embryos and are associated mainly with the mRNP fraction. However, transposon-derived piRNAs, which are more abundant in 0-1h embryos, co-sediment primarily with the polysome fractions. These results suggest that there appears to be a complex interplay among the small RNAs with respect to their polysome-cosedimention pattern during early development inDrosophila.

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