Comparative root proteome analysis of two contrasting wheat genotypes Kharchia-65 (highly salt-tolerant) and PBW-373 (salt-sensitive) for salinity tolerance using LC–MS/MS approach

2021 ◽  
Author(s):  
Renu Yadav ◽  
Anita Rani Santal ◽  
Nater Pal Singh
Keyword(s):  
Salinity Tolerance ◽  
Proteome Analysis ◽  
Salt Tolerant ◽  
Wheat Genotypes ◽  
Root Proteome

scholarly journals Growth and salinity tolerance of wheat genotypes at early vegetative stage

2017 ◽  
Vol 28 (1) ◽  
pp. 12-17
Author(s):  
MS Uddin ◽  
N Jahan ◽  
MA Monim
Keyword(s):  
Salinity Tolerance ◽  
Dry Matter ◽  
High Salinity ◽  
Agricultural Research ◽  
Controlled Environment ◽  
Research Station ◽  
Salt Tolerant ◽  
Wheat Genotypes ◽  
Visual Scoring ◽  
Tolerance Study

Growth and salinity tolerance study of wheat genotypes were conducted at regional agricultural research station to evaluate the selected wheat genotypes against salinity and to classify the wheat genotypes in different salt tolerant group. The experiment was carried out with 12 wheat genotypes under semi-controlled environment (inside plastic greenhouse) and natural light in a randomized complete block (RCBD).The materials were evaluated under control (non-saline) and 16 dS/m salinity level. A significant variation among the genotypes was observed for shoot length under both environments. The lowest reduction (2%) was found from G11 followed by G40 (4%). The genotypes showed differences in production of total shoot dry matter (TDM) at both non-saline and saline conditions.  The relative TDM per plant (% TDM to control condition) appears that three genotypes (G24, G33and G40) produced 90% RTDM. Salt tolerant genotype was found to be less affected at high salinity and could be produced better TDM compared to other genotypes. Three genotypes G24, G33 and G40 exhibited tolerant category. The distribution pattern of the genotypes into various salinity tolerance groups indicates that the overall pattern of behaviors of the genotypes tested remain fairly constant under two methods (based on RTDM and visual scoring).Progressive Agriculture 28 (1): 12-17, 2017

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

scholarly journals Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Salinity Tolerance ◽  
Selection Criteria ◽  
Reproductive Stage ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
North African ◽  
Grain Number ◽  
Selection Indices

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  

scholarly journals Early Growth Stage Characterization and the Biochemical Responses for Salinity Stress in Tomato

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 712
Author(s):  
Md Sarowar Alam ◽  
Mark Tester ◽  
Gabriele Fiene ◽  
Magdi Ali Ahmed Mousa
Keyword(s):  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Salinity Treatment ◽  
Salt Tolerant ◽  
Improvement Program ◽  
Biochemical Basis ◽  
Elevated Salinity ◽  
Tolerance Indices ◽  
Promising Source ◽  
Tomato Genotypes

Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most susceptible genotype. To investigate the possible biochemical basis for salt stress tolerance, we further characterized six tomato genotypes with varying levels of salinity tolerance. A higher increase in proline content, and antioxidants activities were observed for the salt-tolerant genotypes in comparison to the susceptible genotypes. Salt-tolerant genotypes identified in this work herald a promising source in the tomato improvement program or for grafting as scions with improved salinity tolerance in tomato.

scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Salinity Tolerance ◽  
Growth Phase ◽  
In Vitro Selection ◽  
Growth Phases ◽  
Salt Tolerant ◽  
Variable Response ◽  
Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

scholarly journals Phenotyping and molecular marker analysis of WH1105 and Kharchia 65 backcrosses and F4progenies for salinity tolerance

2019 ◽  
Author(s):  
Varsha ◽  
Shikha Yashveer ◽  
Vikram Singh ◽  
Swati Pratap
Keyword(s):  
Salinity Tolerance ◽  
Soil Salinity ◽  
Wheat Variety ◽  
Crop Productivity ◽  
Recurrent Parent ◽  
Salt Tolerant ◽  
Salt Stress Condition ◽  
Initial Salt ◽  
The Cross ◽  
Molecular Marker Analysis

ABSTRACTSoil salinity is a worldwide adverse environmental factor for crop productivity and quality in arid, semiarid and coastal areas. In India, approximately 8.5 million hectare of land area is affected by high salinity (EC ≥ 5 dS m−1). Development of salinity tolerant varieties through marker assisted breeding is most efficient and effective strategy for management of soil salinity. WH 1105 is widely cultivated wheat variety with many agronomically superior qualities but is affected by soil salinity. Two genes (Nax1andNax2) for salinity tolerance were introgressed from Kharchia 65 into the genetic background of WH 1105 through marker assisted backcross breeding. BC1F3, BC2F2and F4generations of the cross WH1105 x Kharchia 65 were evaluated for various morphological traits under initial salt stress condition. On the basis of phenotypic and genotypic variations 44 high yielding plants were selected from the cross. Out of 178 SSRs tested, 30 were found polymorphic for background selection of the foreground selected plants. Cluster tree analysis of parents and all the three generations showed that all the selected plants were inclined toward recurrent parent (WH 1105) indicating higher similarity with the recurrent parent. Four plants were selected as high grain yielding and salt tolerant. These plants could be further backcrossed with the recurrent parent to develop salt tolerant wheat lines.

Genetics of physiological and agronomical traits linked to salinity tolerance in tomato

2021 ◽  
Vol 72 (4) ◽  
pp. 280
Author(s):  
Mohammad Moradi ◽  
Hamid Dehghani ◽  
Sied Zabihallah Ravari
Keyword(s):  
Cell Membrane ◽  
Salinity Tolerance ◽  
Combining Ability ◽  
Relative Water Content ◽  
Gene Action ◽  
Fruit Weight ◽  
Fruit Yield ◽  
Salt Tolerant ◽  
Cell Membrane Stability ◽  
Relative Water

Improvement of tomato (Lycopersicon esculentum L.) for growth in saline soils is a major goal of tomato breeders. The aim of this study was to identify the genetic combining ability and genetics of salinity tolerance in tomato. Plant materials were grown under normal (NG) and salinity stress (SSG) conditions. Results showed that the genetic controlling mechanism of salinity-related traits and fruit weight is complex and that all genetic components of additive, non-additive and maternal are involved. The nature of gene action for fruit weight and salinity-related traits was significantly affected by salinity stress. Dominance and additive gene action were predominant under NG and SSG, respectively. Under NG, the best general combiner parent for fruit weight was P3 (salt-tolerant with moderate fruit yield). Under SSG, P1 (highly salt-tolerant with low fruit yield) was the best general combiner parent for fruit weight and exhibited high genetic combining ability for K+/Na+, lipoxygenase activity, proline, relative water content, total carbohydrate and cell membrane stability. With the high frequency of genes effective in salt tolerance, the P1 parent appeared as the best specific mating partner with other parents under SSG. Simultaneous selection for fruit weight and surrogate traits (cell membrane stability, proline and relative water content) in a population derived from the P1 × P5 (susceptible with high fruit yield) cross could result in a salt-tolerant tomato genotype.

scholarly journals Evaluation of Rice Germplasm under Salt Stress at the Seedling Stage through SSR Markers

2013 ◽  
Vol 3 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M Al-Amin ◽  
MM Islam ◽  
SN Begum ◽  
MS Alam ◽  
M Moniruzzaman ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Salinity Tolerance ◽  
Dry Matter ◽  
Growth Parameters ◽  
Seedling Stage ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Genotypes ◽  
Salt Susceptible

Twenty eight rice germplasms were used for identification of salt tolerant rice genotypes at the seedling stage at the experimental farm and Biotechnology laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during February 2009 to October 2009. Phenotyping for salinity screening of the rice genotypes was done using salinized (EC level 12 dS m-1) nutrient solution in hydroponic system. Genotypes were evaluated for salinity tolerance on 1-9 scale based on seedling growth parameters following modified Standard Evaluation Scoring (SES) of IRRI. Phenotypically, on the basis of SES and % total dry matter (TDM) reduction of the genotypes viz. PBSAL-614, PBSAL-613, PBSAL-730, Horkuch, S-478/3 Pokkali and PBSAL (STL)-15 were found to be salt tolerant; on the other hand Iratom-24, S-653/32, S-612/32, S-604/32, S-633/32, Charnock (DA6), BINA Dhan-6 and S-608/32 were identified as salt susceptible. For genotyping, ten SSR markers were used for polymorphism, where 3 primers (RM127, RM443 and RM140) were selected for evaluation of salt tolerance. In respect of Primer RM127, 7 lines were found salt tolerant and 11 lines were moderately tolerant and 10 lines were susceptible. Nine tolerant, 9 moderately tolerant and 10 susceptible lines were found when the primer RM140 was used and primer RM443 identified 8 lines as tolerant, 9 lines as moderately tolerant and 11 lines as susceptible. Thus, the salt tolerant lines can be used in further evaluation for salinity tolerance and the SSR markers used in this study are proving valuable for identifying salt tolerant genes in marker assisted breeding. Int. J. Agril. Res. Innov. & Tech. 3 (1): 52-59, June, 2013 DOI: http://dx.doi.org/10.3329/ijarit.v3i1.16093

scholarly journals Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569
Author(s):  
Annick Bertrand ◽  
Craig Gatzke ◽  
Marie Bipfubusa ◽  
Vicky Lévesque ◽  
Francois P. Chalifour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Water Content ◽  
Salinity Tolerance ◽  
Osmotic Adjustment ◽  
Recurrent Selection ◽  
Aromatic Amino Acids ◽  
Nacl Stress ◽  
Salt Tolerant

Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

scholarly journals Morphological and Genetic Diversity within Salt Tolerance Detection in Eighteen Wheat Genotypes

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 287 ◽  
Author(s):  
Ibrahim Al-Ashkar ◽  
Ali Alderfasi ◽  
Walid Ben Romdhane ◽  
Mahmoud F. Seleiman ◽  
Rania A. El-Said ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Dry Matter ◽  
Phenotypic Trait ◽  
Salinity Treatment ◽  
Salt Tolerant ◽  
Salt Stress Response ◽  
Wheat Genotypes ◽  
Relative Change

Salinity is a major obstacle to wheat production worldwide. Salt-affected soils could be used by improving salt-tolerant genotypes depending upon the genetic variation and salt stress response of adapted and donor wheat germplasm. We used a comprehensive set of morpho-physiological and biochemical parameters and simple sequence repeat (SSR) marker technique with multivariate analysis to accurately demonstrate the phenotypic and genetic variation of 18 wheat genotypes under salinity stress. All genotypes were evaluated without NaCl as a control and with 150 mM NaCl, until the onset of symptoms of death in the sensitive plant (after 43 days of salinity treatment). The results showed that the relative change of the genetic variation was high for all parameters, heritability (>60%), and genetic gain (>20%). Stepwise regression analysis, noting the importance of the root dry matter, relative turgidity, and their respective contributions to the shoot dry matter, indicated their relevance in improving and evaluating the salt-tolerant genotypes of breeding programs. The relative change of the genotypes in terms of the relative turgidity and shoot dry matter during salt stress was verified using clustering methods. For cluster analysis, the genotypes were classified into three groups: tolerant, intermediate, and sensitive, representing five, six, and seven genotypes, respectively. The morphological and genetic distances were significantly correlated based on the Mantel test. Of the 23 SSR markers that showed polymorphism, 17 were associated with almost all examined parameters. Therefore, based on the observed molecular marker-phenotypic trait association, the markers were highly useful in detecting tolerant and sensitive genotypes. Thus, it considers a helpful tool for salt tolerance through marker-assisted selection.

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