scholarly journals Unlocking the bacterial contact-dependent antibacterial activity to engineer a biocontrol alliance of two species from natural incompatibility to artificial compatibility

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Qianhua Wu ◽  
Bozhen Wang ◽  
Xi Shen ◽  
Danyu Shen ◽  
Bingxin Wang ◽  
...  
Keyword(s):  
Species Interactions ◽  
Plant Growth Promoting Rhizobacteria ◽  
Single Species ◽  
Type Iv Secretion ◽  
Lysobacter Enzymogenes ◽  
Type Iv ◽  
Promising Strategy ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Antibacterial And Antifungal

AbstractPlant growth-promoting rhizobacteria (PGPR) contain various biocontrol bacteria with broad-spectrum antimicrobial activity, and their single species has been extensively applied to control crop diseases. The development of complex biocontrol community by mixing two or more PGPR members together is a promising strategy to enlarge the efficacy and scope of biocontrol. However, an effective method to assess the natural compatibility of PGPR members has not yet been established to date. Here, we developed such a tool by using the bacterial contact-dependent antibacterial activity (CDAA) as a probe. We showed that the CDAA events are common in two-species interactions in the four selected representative PGPRs, represented by the incompatible interaction of Lysobacter enzymogenes strain OH11 (OH11) and Lysobacter antibioticus strain OH13 (OH13). We further showed that the CDAA between OH11 and OH13 is jointly controlled by a contact-dependent killing device, called the type IV secretion system (T4SS). By deleting the respective T4SS synthesis genes, the T4SS in both strains was co-inactivated and this step unlocked  their natural CDAA, resulting in an engineered, compatible mutant alliance that co-displayed antibacterial and antifungal activity. Therefore, this study reveals that releasing bacterial CDAA is effective to rationally engineer the biocontrol community.

scholarly journals Metatranscriptomics and nitrogen fixation from the rhizoplane of maize plantlets inoculated with a group of PGPRs

2018 ◽  
Author(s):  
Lorena Jacqueline Gómez-Godínez ◽  
Ernesto Ormeño-Orrillo ◽  
Esperanza Martínez-Romero
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Differential Expression Analysis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Single Species ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Nitrogen Fixing ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTThe free-living soil bacteria that are beneficial for the growth of plants are known as plant growth-promoting rhizobacteria (PGPR). In this work, a multi-species of PGPR bacteria inoculant was designed, which included nitrogen-fixing strains such as Rhizobium phaseoli, Sinorhizobium americanum and Azospirillum brasilense, as well as other plant growth promoting bacteria such as Bacillus subtillis and Methylobacterium extorquens. The multi-species community exerted a beneficial effect on plant seedlings when it was inoculated, greater than the effect observed when inoculating each bacteria individually. Acetylene reduction of maize roots was recorded with the multi-species inoculant, which suggests that nitrogen fixation occurred under these conditions. To analyze the contributions of the different nitrogen-fixing bacteria that were inoculated, a metatranscriptomic analysis was performed. The differential expression analysis revealed that the predominantly nif transcripts of Azospirillum are overexpressed, suggesting that it was responsible for nitrogen fixation in maize. Overall, we analyzed the interaction of a synthetic community, suggesting it as an option, for future formulations of biofertilizers.IMPORTANCEWhile nodulation processes and nitrogen fixation by rhizobia have been well studied, little is known about the interaction between rhizobia and non-leguminous plants such as maize, which is used as a model for this study. Nitrogen fixation in cereals is a long searched goal. Instead of single species inoculants, multi-species inoculation may be more efficient to promote plant growth and fix nitrogen. Metatrascriptomes allowed us to recognize the bacteria responsible for nitrogen fixation in plant rootlets. The study of the function of certain genes may help to understand how microorganisms interact with the root plant, as well as allow a better use of microorganisms for the generation of novel biofertilizers using microbial consortia.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

A Study on the Farmers’ Awareness and Acceptance of Biofertilizers in Kottayam District

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 425-431
Author(s):  
Subin Thomas ◽  
Dr. M. Nandhini
Keyword(s):  
Organic Matter ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nutrient Cycle ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Structured Questionnaire ◽  
Area Data

Biofertilizers are fertilizers containing microorganisms that promote plant growth by improving the supply of nutrients to the host plant. The supply of nutrients is improved naturally by nitrogen fixation and solubilizing phosphorus. The living microorganisms in biofertilizers help in building organic matter in the soil and restoring the natural nutrient cycle. Biofertilizers can be grouped into Nitrogen-fixing biofertilizers, Phosphorous-solubilizing biofertilizers, Phosphorous-mobilizing biofertilizers, Biofertilizers for micro nutrients and Plant growth promoting rhizobacteria. This study conducted in Kottayam district was intended to identify the awareness and acceptance of biofertilizers among the farmers of the area. Data have been collected from 120 farmers by direct interviews with structured questionnaire.

Evaluating efficacy of plant growth promoting rhizobacteria and potassium fertilizer on spinach growth under salt stress

2020 ◽  
Vol 52 (4) ◽  
Author(s):  
Muhammad Zafar-Ul-Hye ◽  
Fiza Mahmood ◽  
Subhan Danish ◽  
Shahid Hussain ◽  
Mehreen Gul ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Potassium Fertilizer ◽  
Plant Growth Promoting ◽  
Growth Promoting
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