The role of gingival crevicular fluid and salivary interstitial collagenases in human periodontal diseases

1990 ◽  
Vol 35 ◽  
pp. S193-S196 ◽  
Author(s):  
T. Sorsa ◽  
K. Suomalainen ◽  
V.-J. Uitto
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Crevicular Fluid

scholarly journals Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

2011 ◽  
Vol 31 (6) ◽  
pp. 343-352 ◽  
Author(s):  
Sema Becerik ◽  
Beral Afacan ◽  
Veli Özgen Öztürk ◽  
Harika Atmaca ◽  
Gülnur Emingil
Keyword(s):  
Healthy Subjects ◽  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Inflammatory Marker ◽  
Aggressive Periodontitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Probing Depth ◽  
Crevicular Fluid ◽  
First Time

Aim:The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases.Material and methods:Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA).Results:CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p< 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p< 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p< 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p< 0.008)Conclusions:Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.

scholarly journals Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Oya Türkoğlu ◽  
Elif Azarsız ◽  
Gülnur Emingil ◽  
Necil Kütükçüler ◽  
Gül Atilla
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Study Groups ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Proteinase 3 ◽  
Cathepsin C ◽  
Probing Depth ◽  
Inflammatory Processes ◽  
Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

Diagnosis of Periodontal Diseases: Reaction Paper

1991 ◽  
Vol 5 (1) ◽  
pp. 37-40 ◽  
Author(s):  
M.J. Novak
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Diagnostic Techniques ◽  
Disease States ◽  
Specificity And Sensitivity ◽  
Biochemical Analyses ◽  
Clinical Measurements ◽  
The Individual ◽  
Crevicular Fluid ◽  
Technological Improvements

With the recent description of 12 different forms and sub-forms of periodontitis by the World Workshop in Clinical Periodontics (1989), increased emphasis has been placed on diagnosis. Dr. Ranney's review addressed the specificity and sensitivity of current diagnostic tests with respect to their ability to differentiate between health and disease and between the individual disease states. Although considerable microbiologic and immunologic data have been accumulated in the past decade, very little of this information has proved to be sufficiently sensitive to be of use in differential diagnosis. Clinical measurements provide us with an insensitive, retrospective analysis of what has already occurred but allow us to diagnose disease based on its natural history. Measures of attachment levels, by use of conventional probes, are only sufficiently sensitive indicators of periodontitis when as much as 20-30% of attachment has already been lost. Current technological improvements in probing measurements and radiographic assessment may increase sensitivity in this area. Future improvements in diagnostic techniques will occur with the advent of sensitive biochemical analyses of gingival crevicular fluid. These assays will provide a more objective analysis of inflammation and, in time, will provide sufficient sensitivity to allow for differentiation between and among the various forms of periodontal disease. Future directions in diagnosis will focus on the identification of disease-susceptible individuals and the prediction of future periodontal breakdown.

scholarly journals Periodontitis Vaccine Decreases Local Prostaglandin E2 Levels in a Primate Model

2004 ◽  
Vol 72 (2) ◽  
pp. 1166-1168 ◽  
Author(s):  
Frank A. Roberts ◽  
Laura S. Houston ◽  
Sheila A. Lukehart ◽  
Lloyd A. Mancl ◽  
G. Rutger Persson ◽  
...  
Keyword(s):  
Bone Loss ◽  
Gingival Crevicular Fluid ◽  
Prostaglandin E ◽  
Primate Model ◽  
Necrosis Factor Alpha ◽  
Periodontal Destruction ◽  
Factor Alpha ◽  
Crevicular Fluid ◽  
Necrosis Factor

ABSTRACT Interleukin-1β, tumor necrosis factor alpha, prostaglandin E2 (PGE2), and Porphyromonas gingivalis-specific immunoglobulin G levels in gingival crevicular fluid were measured in primates immunized with a P. gingivalis vaccine followed by ligature-induced periodontitis. Only PGE2 levels were dramatically suppressed (P < 0.0001) in immunized animals versus controls. A significant correlation (P < 0.027) was also found between PGE2 levels and decreased bone loss scores. This study presents the first evidence of a potential mechanism involved in periodontitis vaccine-induced suppression of bone loss in a nonhuman primate model and offers insight into the role of PGE2 in periodontal destruction.

scholarly journals Cytokines in crevicular fluid and orthodontic force: a systematic review

2017 ◽  
Vol 32 (2) ◽  
pp. 104 ◽  
Author(s):  
Susiane Allgayer ◽  
Luciane Macedo De Menezes ◽  
Mariana Roennau Lemos Rinaldi
Keyword(s):  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Cellular Level ◽  
Reference Tracking ◽  
Mesh Terms ◽  
Cytokine Levels ◽  
Synergistic Regulation ◽  
Handling Methods ◽  
Crevicular Fluid

OBJECTIVE: This paper aimed to analyze studies in the literature addressing the role of cytokines in the gingival crevicular fluid (GCF) in the orthodontic treatment.METHODS: Several databases were surveyed using both MESH terms and free terms. Additional studies were obtained by reference tracking. This review was registered in PROSPERO, and the procedures foreseen by its statement were followed. Data were obtained from the included studies addressing the orthodontic mechanics, GCF sampling/handling methods, and cytokine measurements. Clinical studies written in English were browsed. Papers were selected by one reviewer and checked by a second investigator.RESULTS: A total of 115 articles were identified, among which 25 were selected for detailed analysis. Common drawbacks consisted mainly of inadequacies in the study design (e.g. short duration and small number of study subjects). The most consistent result was a peak of cytokine levels at 1 d. Associations were observed between prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) and pain, velocity of tooth movement, and treatment mechanics. Interleukin-1b and PGE2 showed different patterns of up-regulation, with IL-1β being more responsive to mechanical stress and PGE2 more responsive to synergistic regulation of IL-1β and mechanical force. The results support the use of light continuous forces for orthodontic treatment.CONCLUSION: There was a tendency of maintenance of relatively high IL-1β levels for longer periods with the use of light continuous forces, which might decrease the frequency of activation. These outcomes provide evidence at the cellular level for the utilization of light continuous forces.

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