Using molecular techniques applied to beneficial microorganisms as biotechnological tools for controlling agricultural plant pathogens and pest

Author(s):  
Liliana Aguilar-Marcelino ◽  
Pedro Mendoza-de-Gives ◽  
Laith Khalil Tawfeeq Al-Ani ◽  
María Eugenia López-Arellano ◽  
Olga Gómez-Rodríguez ◽  
...  
2021 ◽  
Vol 6 (1) ◽  
pp. 153-159
Author(s):  
E.O Akinyelure ◽  
◽  
D.A. Machido ◽  
H. I. Atta

Bacillus thuringiensis (Bt) is the organism that is used most frequently in biological pest management, which is distinguished by the capacity to possess crystalline inclusions throughout the sporulation phase. There is an increasing need to use biological control in controlling plant pathogens due to the inherent advantages. However, the detection of Bt has become more time consuming and cumbersome due to the numerous available crystal genes. The goal of the study was to isolate strains of Bacillus thuringiensis from the soil, characterise the isolates using the transcriptional regulator, XRE gene and the crystal proteins cry2gene and compare the efficiency of these two biomarkers in identifying Bt species. Five different Bacillus thuringiensis strains were isolated from soil samples in Zaria, Nigeria. Polymerase chain reaction was used to detect the existence of the cry2 and XRE genes. Four (80%) of the five isolates harboured the XRE genes, while none (0%) harboured the cry2 genes. This observation is a likely indication that the XRE gene is a reliable biomarker in the identification of Bt isolates from environmental samples. In order to ensure speed and reproducibility in the detection of Bt from environmental samples, molecular techniques targeting the XREgene are recommended. Keywords: Bacillus thuringiensis; transcriptional regulator, XRE; crystal protein, cry2


2020 ◽  
Vol 58 (1) ◽  
pp. 277-311 ◽  
Author(s):  
Erin Rosskopf ◽  
Francesco Di Gioia ◽  
Jason C. Hong ◽  
Cristina Pisani ◽  
Nancy Kokalis-Burelle

The loss of methyl bromide as a soil fumigant and minimal advances in the development and registration of new chemical fumigants has resulted in a resurgence of interest in the application of organic amendments (OAs) for soilborne plant pathogen and plant-parasitic nematode management. Significant progress has been made in the characterization of OAs, application of strategies for their use, and elucidation of mechanisms by which they suppress soilborne pests. Nonetheless, their utility is limited by the variability of disease control, expense, and the logistics of introducing them into crop production systems. Recent advances in molecular techniques have led to significant progress in the elucidation of the role of bacteria and fungi and their metabolic products on disease suppression with the addition of OAs. Biosolarization and anaerobic soil disinfestation, developed to manipulate systems and favor beneficial microorganisms to maximize their impact on plant pathogens, are built on a strong historical research foundation in OAs and the physical, chemical, and biological characteristics of disease-suppressive soils. This review focuses on recent applications of OAs and their potential for the management of soilborne plant pathogens and plant-parasitic nematodes, with emphasis primarily on annual fruit and vegetable production systems.


2006 ◽  
Vol 59 ◽  
pp. 132-136 ◽  
Author(s):  
M. Braithwaite ◽  
C.F. Hill ◽  
S. Ganev ◽  
J.M. Pay ◽  
H.G. Pearson ◽  
...  

During 2003 and 2004 fortyfive randomly selected wholesale and retail plant nurseries were surveyed for plant diseases The plant families Agavaceae Annonaceae Arecaceae Bromeliaceae Cycadaceae and Musaceae were targeted Plants were examined in situ for disease symptoms as well as samples being collected for laboratory analyses Fungi were identified using morphological characteristics and where necessary with molecular techniques The survey resulted in a range of fungi being identified from the target plants These fungi ranged from saprophytes to plant pathogens some of which may have undesirable effects on New Zealands biodiversity or economy Many new host/pathogen records were observed and several fungi were detected for the first time in New Zealand This paper presents and discusses the results of these findings


2022 ◽  
Vol 951 (1) ◽  
pp. 012002
Author(s):  
A Khakimov ◽  
I Salakhutdinov ◽  
A Omolikov ◽  
S Utaganov

Abstract As it is known, a significant part of the yield of agricultural crops is lost due to harmful organisms, including diseases. The article reveals the data on the widespread types of plant diseases (rot, wilting, deformation, the formation of tumors, pustules, etc.) and their symptoms. Early identification of the pathogen type of plant infection is of high significance for disease control. Various methods are used to diagnose pathogens of disease on plant. This article discusses the review of the literature data on traditional methods for diagnosis of plant pathogens, such as visual observation, microscopy, mycological analysis, and biological diagnostics or the use of indicator plants. Rapid and reliable detection of plant disease and identification of its pathogen is the first and most important stage in disease control. Early identification of the cause of the disease allows timely selection of the proper protection method and ensures prevention of crop losses. There are a number of traditional methods for identifying plant diseases, however, in order to ensure the promptness and reliability of diagnostics, as well as to eliminate the shortcomings inherent in traditional diagnostics, in recent years, new means and technologies for identifying pathogens have been developed and introduced into practice. As well as the article provides information on such innovative methods of diagnosis of diseases and identification of their pathogens, which are used widely in the world today, such as immunodiagnostics, molecular-genetic (and phylogenetic) identification, mass spectrometry, etc.


2003 ◽  
Vol 83 (Special Issue) ◽  
pp. 331-336 ◽  
Author(s):  
R. D. Reeleder

The role of biodiversity as it affects the control of soil-borne fungal pathogens is discussed. Soil-borne fungal plant pathogens have often proven difficult to manage with conventional methods of disease control. Nonetheless, researchers have characterized several naturally occurring “disease-suppressive” soils where crop loss from disease is less than would otherwise be expected. Suppressive soils can also result from the incorporation of various amendments into soil. In most cases, disease control in such soils has been shown to be biological in nature; that is, soil organisms appear to directly or indirectly inhibit the development of disease. Increased knowledge of the identity and functioning of these organisms may support the development of techniques that can be used to develop suppressiveness in soils that are otherwise disease-conducive. Populations of pathogens themselves have been shown to exhibit considerable genetic diversity; the ability of populations to respond to disease control measures should be considered when developing a management strategy. New molecular techniques can be exploited to better characterize soil communities, including the pathogens themselves, as well as community responses to various disease control options. The contributions of Canadian researchers to these areas are discussed and models for further study are proposed. Key words: Biocontrol, molecular technologies, functional diversity, integrated pest management


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