Biochemical parameters of Spodoptera frugiperda (J. E. Smith) treated with citronella oil (Cymbopogon winterianus Jowitt ex Bor) and its influence on reproduction

2016 ◽  
Vol 118 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Cristiane Thalita dos Santos Silva ◽  
Valéria Wanderley-Teixeira​ ◽  
Franklin Magliano da Cunha ◽  
José Vargas de Oliveira ◽  
Kamilla de Andrade Dutra ◽  
...  
2008 ◽  
Vol 24 ◽  
pp. 1401 ◽  
Author(s):  
Adriana Mascarette Labinas ◽  
Wilson Badiali Crocomo

Existem muitos compostos extraídos de plantas, denominados aleloquímicos, que alteram a biologia e o compotamento dos insetos. Entre essas substâncias os terpenóides são eficientes inibidores do crescimento larval, reprodução e alimentação. Esse trabalho, realizado na Universidade Estadual Paulista, Botucatu, Estado de São Paulo, procurou verificar a bioatividade do óleo essencial da citronela, Cymbopogon winterianus Jowitt (Poaceae) (rico em citronelal e citronelol) sobre Spodoptera frugiperda. Foram realizados dois bioensaios. No primeiro, folhas de milho com 10 cm de comprimento e 4 cm de largura foram divididas em 3 partes iguais. Uma das partes de uma das extremidades foi mergulhada em soluções contendo 1,0, 0,5, 0,1, 0,05, 0,01, 0,005 e 0,001% de óleo essencial de citronella com 0,05% do emulsificante Tween 20, a outra parte foi mergulhada em água com 0.05% desse emulsificante. Quatro larvas recém-eclodidas foram colocadas no centro de cada folha. No segundo ensaio, superfícies de vidro (placas de Petri) foram tratadas com soluções contendo 1,0, 0,5, 0,1, 0,05 % do óleo essencial de citronela e 0,05% do emulsificante Tween 20, mais um tratamento testemunha (água com 0,05% emulsificante). Após a secagem da superfície, foram colocadas em cada placa de vidro, 10 lagartas recém-eclodidas e cobertas com filme plástico. Os resultados mostraram que o óleo essencial de citronela apresenta ação inseticida e de repelência para lagartas de S. frugiperda.


2018 ◽  
Vol 10 (6) ◽  
pp. 147
Author(s):  
Rinaldi Salea ◽  
Stevanus Hiendrawan ◽  
Erna Subroto ◽  
Bambang Veriansyah ◽  
Raymond R. Tjandrawinata

Objective: Optimum condition for the extraction of citronella oil from citronella (Cymbopogon winterianus) using supercritical carbon dioxide (SC-CO2) was investigated.Methods: In order to determine the optimum extraction condition, a Taguchi experiment with L9 orthogonal array design was used. Effects of pressure, temperature and dynamic extraction time on citronella oil yield were investigated at levels ranging between 10-15 MPa, 35-45 °C and 60-180 min, respectively.Results: The highest citronella oil yield (3.206%) was achieved at a factor combination of 15 MPa, 50 °C and 180 min. The obtained citronella oil yield from SC-CO2 extraction was higher than that of percolation as the solvent extraction method using ethanol, which gave a citronella oil yield of 1.4%. The experimental oil yield at optimum condition was in accordance to the values predicted by a computational process using Taguchi method. Analysis of variance (ANOVA) with 95% confidence interval indicates that extraction temperature is the most significant factor in maximizing citronella oil yield, followed by dynamic extraction time and pressure.Conclusion: Optimization process for oil yield from SC-CO2extraction of citronella (Cymbopogon winterianus) was successfully performed using Taguchi L9 orthogonal array design. This study demonstrates that Taguchi method was able to simplify the experimental procedure of SC-CO2 process. 


2020 ◽  
Vol 2020 ◽  
pp. 1-5 ◽  
Author(s):  
Willy Tirza Eden ◽  
Dante Alighiri ◽  
Kasmadi Imam Supardi ◽  
Edy Cahyono

This study examines the active component of Cymbopogon winterianus (Java citronella) oil, as a green mosquito repellent, obtained through a steam distillation method. Java citronella oil, which contains citronellol, citronellal, and geraniol, was isolated by batch vacuum fractional distillation, and their effect was tested against the dengue fever (DF) vector, known as Aedes aegypti. Furthermore, air freshener gels were formulated with Java citronella oil, carrageenan, gum, sodium benzoate, ethylene glycol, polysorbate 20, sodium chloride, and distilled water, at varying concentrations. The results show that formula I has the best controlled release evaporation for citronellal, citronellol, and geraniol, as well as the best storage time of 16.82 days and 12.77 days for geraniol and citronellol, respectively. The most significant specific gravity (0.0136) was recorded in formula V, while gel formula I exhibited the highest level of instability at 35°C, with a syneresis value of 77.11% in t=72 h and pH 5.33. In addition, formula IV at 5°C demonstrated the highest syneresis (75.34%) in t=72 h, with pH 7.04, while a peak viscosity of 100,958 cP was recorded in formula IV. The repellent activity of each active component was measured based on the period of protection conferred against the bites of Aedes aegypti within one hour, and the results showed geraniol and citronellol, with respective activity of 78.00%±4.83 and 77.34%±3.57, as the most effective.


1985 ◽  
Vol 104 (2) ◽  
pp. 477-479 ◽  
Author(s):  
E. V. S. Prakasa Rao ◽  
M. Singh ◽  
R. S. Ganesha Rao ◽  
S. Ramesh

Java citronella (Cymbopogon winterianus Jowitt) is an important perennial aromatic grass cultivated in different parts of India. The steam-volatile essential oil from the citronella herb is widely used in perfumery, in manufacture of deodorants and mosquito repellent creams and in flavouring beverages. Systematic cultivation of this crop is being taken up and application of large quantities of N fertilizers was found to increase yield (Narayana et al. 1975; Bommegowda, 1978; Singh, Pathak & Bodoloi, 1980; Prakasa Rao et al. 1983). However, the efficiency of N utilization by this crop was found to be quite poor (Prakasa Rao et al. 1983). No systematic data are available in India on the effect of harvest season and N fertilizers on the principal constituents of citronella oil. Therefore the present experiment was made with two objectives: (i) to determine the effect of harvest season and N fertilizers on the essential oil concentration and the principal constituents of the oil which determine the oil quality, and (ii) to determine the effect of neem cake, an indigenous cheap material which is reported to have nitrification inhibitory properties, on the herb and essential oil yields of citronella.


Author(s):  
Edy Cahyono ◽  
Harno Dwi Pranowo ◽  
Muchalal Muchalal ◽  
Triyono Triyono

Citronellal 97,3% has been isolated from Java citronella oil (Cymbopogon winterianus) from Yogyakarta Indonesia by fractional distillation under reduced pressure (5 cmHg, 110-120 oC). Citronellal has two optical isomerics that can be separated by capillary column of chiral GC phase. Enantioselective capillary GC with heptakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl)-β-cyclodextrin (β-DEX-225) as stationary phase has been used for analysis of the enantiomers ratio of citronellal. The analysis of enantiomer ratio showed that citronellal contain of 88.21% ee of (R)-(+)-citronellal. Physical properties of isolated citronellal showed that the compound was (+) enantiomer. Structure identification of citronellal was carried out by GC-MS, IR, and 1H NMR, resulted identical fragment and spectra with standard citronellal. Theoretical study with semiempirical-AM1 method showed that energy of (R)-(+)-citronellal on the β-DEX 225 was lower than its (S)-(-)-citronellal.


2021 ◽  
Vol 4 (1) ◽  
pp. 63-71
Author(s):  
Ana Agustina ◽  
Maryam Jamilah

Minyak atsiri dapat dijumpai di berbagai jenis tanaman yang tersebar di seluruh dunia. Indonesia setidaknya memiliki 40 jenis tanaman penghasil minyak atsiri komersial. Minyak atsiri memiliki berbagai manfaat, yaitu sebagai bahan baku kosmetik, obat-obatan, parfum, lilin, dan flavor. Salah satu atsiri yang diproduksi di Indonesia adalah citronella oil, citronella oil termasuk salah satu jenis atsiri yang populer digunakan sebagai anti-nyamuk, anti-jamur, dan anti-oksidan. Meskipun di Indonesia sudah berkembang luas petani yang berkebun serai wangi dan mengolahnya hingga menghasilkan minyak, akan tetapi masih banyak di antara mereka yang memproduksi citronella oil yang belum memenuhi standar. Oleh sebab itu, perlu adanya kajian mengenai faktor-faktor yang mempengaruhi kualitas citronella oil yang dihasilkan oleh usaha perseorangan agar dapat memenuhi standar industri. Untuk mengetahui kandungan citronella oil, dilakukan pengujian GC-MS. Hasil penelitian menunjukkan bahwa citronella oil yang dihasilkan memiliki kadar sitronellal yang lebih rendah dibandingkan industri besar, yaitu 11.37%. Sedangkan standar minimal untuk sitronelal adalah 35%, hal ini diduga berkaitan dengan umur panen, kualitas tempat tumbuh, kualitas mesin, dan pretreatment.


2020 ◽  
Vol 3 (1) ◽  
pp. 22
Author(s):  
Danar Dono ◽  
Yusup Hidayat ◽  
Tarkus Suganda ◽  
Syarif Hidayat ◽  
Neneng Sri Widayani

An important pest in corn plantation recently reported in Indonesia was Spodoptera frugiperda, a polyphagous pest that have a fast spread rate and caused high damage on young corn. One control that able to use is botanical insecticide. This experiment aims to determine the toxicity of neem, jatropha, clove, and citronella oil (concentrations of 0.1% and 0.05%) against S. frugiferda in corn. Tests carried out using the feeding assay or residue on corn leaves (size 5 x 5 cm). Then, the leaf treatment was given to 10 larvae of S. frugiferda (instars 2) for 48 hours. After that, the feed replaced with untreated feed. The test results showed that concentration neem oil of 0.1% caused the highest mortality of S. frugiferda. Neem oil exposure is also able to inhibit the time of larval development, reduce the percentage of feed consumption, and the weight of pupa the S. frugiferda. Other vegetable oils exposure gave lower mortality in the test insects and some treatments were not significantly different from the controls on the parameters of larval development, pupae weight, and percentage of feed consumption. Neem oils showed strong toxicity against S. frugiperda larvae with  Lethal Concentration 50 (LC50) of 0.079 % (CI (95%) 0.066 – 0.095%) and LC95 of 0.338% (CI (95%) 0.024-0.567%).


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