scholarly journals Antimycotic Treatment of Oral Candidiasis in Warfarin Users

2020 ◽  
Author(s):  
Ditte B. Iversen ◽  
Maja Hellfritzsch ◽  
Tore B. Stage ◽  
Rune M. Aabenhus ◽  
Bent S. Lind ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
Antimycotic Treatment

Efek Proteksi Ekstrak Etanol Stichopus hermanii Terhadap Jumlah Limfosit pada Tikus yang Terpapar Asap Rokok dan Diinduksi Candida albicans

DENTA ◽  
2015 ◽  
Vol 9 (2) ◽  
pp. 146
Author(s):  
Auliasari Yunanda ◽  
Syamsulina Revianti ◽  
Isidora Karsini
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Control Group ◽  
Post Test

<p><strong><em>Latar Belakang: </em></strong>Merokok berhubungan dengan jamur rongga mulut yang dapat mengakibatkan <em>oral candidiasis</em>. <em>Stichopus hermanii</em><em> </em>mengandung efek antioksidan, antifungi dan immunostimulator. <strong><em>Tujuan: </em></strong>Mengevaluasi efek proteksi ekstrak <em>Stichopus hermanii </em>terhadap jumlah limfosit pada tikus Wistar yang terpapar asap rokok dan diinduksi <em>C.albicans.<strong> Bahan dan Metode: </strong></em>Rancangan penelitian ini adalah <em>post test-only control group</em> <em>design</em><strong><em>. </em></strong>42 ekor tikus Wistar jantan, dibagi menjadi 7 kelompok, Kelompok1 (saline 0,1mL, udara segar, CMC-Na 0,2%), Kelompok2 (saline 0,1mL, asap rokok, CMC-Na 0,2%), Kelompok3 (<em>C.albicans </em>0,1mL, udara segar, CMC-Na 0,2%), Kelompok4 (<em>C.albicans </em>0,1mL, asap rokok, CMC-Na 0,2%), Kelompok5 (saline 0,1mL, asap rokok, ekstrak <em>Stichopus hermanii</em> 0,02mg/kgBB), Kelompok6 (<em>C.albicans</em> 0,1mL, udara segar, ekstrak <em>Stichopus hermanii </em>0,02mg/kgBB), Kelompok7 (<em>C.albicans </em>0,1 mL, asap rokok, ekstrak <em>Stichopus hermanii </em>0,02mg/kgBB). Tikus Wistar diinduksi <em>C.albicans</em> 1 minggu, terpapar asap rokok 8 minggu, dan diberi ekstrak <em>Stichopus hermanii</em> 8 minggu. Selanjutnya, tikus Wistar dikorbankan setelah 2 bulan perlakuan. Jumlah limfosit dihitung melalui metode hapusan darah dengan <em>different counting</em> dibawah mikroskop cahaya dengan pembesaran 1000x. Data yang diperoleh dianalisis menggunakan uji <em>Kruskal-Wallis</em> dan <em>Mann-Whitney</em>.<strong><em> Hasil:</em></strong> Kelompok yang terpapar asap rokok dan diinduksi C.albicans memiliki dapat menurunkan jumlah limfosit, kelompok suplementasi menggunakan ekstrak ethanol <em>Stichopus hermanii</em> dapat meningkatkan jumlah limfosit<em>. </em><strong><em>S</em></strong><strong><em>impulan:</em></strong><strong> </strong>Suplementasi ekstrak <em>Stichopus hermanii</em> memiliki efek protektif untuk memicu proliferasi limfosit pada tikus Wistar setelah paparan asap rokok dan induksi <em>C.albicans</em>.</p>

Opportunistic infections among people living with HIV at reference health care centers in Morocco, 2015 (Preprint)

2019 ◽  
Author(s):  
Noureddine Sakhri ◽  
Fatima Zahra Meski ◽  
SOUMIA TRIKI
Keyword(s):  
Health Care ◽  
Opportunistic Infections ◽  
Oral Candidiasis ◽  
Clinical Stage ◽  
Bivariate Analysis ◽  
People Living With Hiv ◽  
Cross Sectional Survey ◽  
Pneumocystis Jiroveci ◽  
Cross Sectional ◽  
Health Care Centers

BACKGROUND Morbidity and mortality in HIV disease is due to immune-suppression leading to life-threatening opportunistic infections (OIs) during the natural course of the disease. In 2015, the HIV prevalence is low in general population and concentrated among key populations. OBJECTIVE This study aimed to assess the prevalence and CD4 correlates of OIs among adult HIV-infected patients attending antiretroviral health care in Morocco, during 2015. METHODS We conducted a cross-sectional survey among all adult PLHIV for admitted in the health care centers during 2015, who had acquired infection disease. Patients’ opportunistic infection status was determined through clinical diagnosis and laboratory investigations. CD4 count was determined using flow cytometry technique. The clinical stage of HIV was identified by the classification of Centers for Disease Control and Prevention (CDC). We collected Socio-demographic and clinical data from patients’ medical records. We performed statistical analysis by using Epi-Info 7.2.0.1 software. The appropriate test was applied, bivariate analysis was made and the differences were significant when p<.05. RESULTS 299 HIV-infected cases were included; 53% were males. The most represented age group was 25-34 years (36.1%). The mean age of the cases was 38.7 ± 16.8. The prevalence of OIs was 47.8%. Tuberculosis (65/299, 21.7%), Pneumocystis jiroveci pneumonia (40/299, 13.4%) and Oral candidiasis (22/299, 7.4%) were the most frequently observed OIs. CONCLUSIONS Tuberculosis, pneumocystis and oral candidacies were the leading OIs, encountered by HIV-infected cases. Preventive measures and early diagnosis of HIV associated to OIs are crucial.

scholarly journals Novel Aggregation Properties of Candida albicans Secreted Aspartyl Proteinase Sap6 Mediate Virulence in Oral Candidiasis

2015 ◽  
Vol 83 (7) ◽  
pp. 2614-2626 ◽  
Author(s):  
Rohitashw Kumar ◽  
Darpan Saraswat ◽  
Swetha Tati ◽  
Mira Edgerton
Keyword(s):  
Candida Albicans ◽  
Epithelial Cells ◽  
Oral Candidiasis ◽  
Oral Microbiome ◽  
Proteinase Activity ◽  
Adhesion Properties ◽  
Content Type ◽  
Oral Epithelial Cells ◽  
Oral Epithelial ◽  
Cell Cell

Candida albicans, a commensal fungus of the oral microbiome, causes oral candidiasis in humans with localized or systemic immune deficiencies. Secreted aspartic proteinases (Saps) are a family of 10 related proteases and are virulence factors due to their proteolytic activity, as well as their roles in adherence and colonization of host tissues. We found that mice infected sublingually withC. albicanscells overexpressing Sap6 (SAP6OE and a Δsap8strain) had thicker fungal plaques and more severe oral infection, while infection with the Δsap6strain was attenuated. These hypervirulent strains had highly aggregative colony structurein vitroand higher secreted proteinase activity; however, the levels of proteinase activity ofC. albicansSaps did not uniformly match their abilities to damage cultured oral epithelial cells (SCC-15 cells). Hyphal induction in cells overexpressing Sap6 (SAP6OE and Δsap8cells) resulted in formation of large cell-cell aggregates. These aggregates could be produced in germinated wild-type cells by addition of native or heat-inactivated Sap6. Sap6 bound only to germinated cells and increasedC. albicansadhesion to oral epithelial cells. The adhesion properties of Sap6 were lost upon deletion of its integrin-binding motif (RGD) and could be inhibited by addition of RGD peptide or anti-integrin antibodies. Thus, Sap6 (but not Sap5) has an alternative novel function in cell-cell aggregation, independent of its proteinase activity, to promote infection and virulence in oral candidiasis.

scholarly journals Oral Candidiasis and COVID-19 in Users of Removable Dentures: Is Special Oral Care Needed?

Gerontology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Laura Silva Jerônimo ◽  
Rafael Paschoal Esteves Lima ◽  
Thaís Yumi Umeda Suzuki ◽  
José Augusto César Discacciati ◽  
Cláudia Lopes Brilhante Bhering
Keyword(s):  
Mechanical Ventilation ◽  
Candida Albicans ◽  
Oral Health ◽  
Early Diagnosis ◽  
Elderly Patients ◽  
Oral Candidiasis ◽  
Oral Care ◽  
Antifungal Treatment ◽  
Immunocompromised Patients ◽  
Appropriate Care

Elderly patients with systemic disorders and immunocompromised patients seem to have a higher risk of developing morbidity from COVID-19. <i>Candida albicans</i> (<i>C. albicans</i>) is a potentially dangerous pathogen for these patients, especially for denture wearers with prosthetic stomatitis who require mechanical ventilation. <i>C. albicans</i> infection, the main candidiasis infection associated with denture wear, can complicate COVID-19 and increase the associated morbidity and mortality. Therefore, early diagnosis of <i>C. albicans</i> infection in COVID-19 patients is important to establish more effective antifungal treatment methods and prophylaxis strategies. Hospitalized COVID-19 patients should undergo an oral examination to assess their oral health, and those with poor oral health should receive the appropriate care and monitoring.

scholarly journals Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 998
Author(s):  
Virgínia Barreto Lordello ◽  
Andréia Bagliotti Meneguin ◽  
Sarah Raquel de Annunzio ◽  
Maria Pía Taranto ◽  
Marlus Chorilli ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Enterococcus Faecium ◽  
Potato Starch ◽  
Oral Candidiasis ◽  
Candida Spp ◽  
Sem Images ◽  
Control Film ◽  
In Vitro Antifungal Activity

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

scholarly journals Efficacy of Toluidine Blue—Mediated Antimicrobial Photodynamic Therapy on Candida spp. A Systematic Review

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 349
Author(s):  
Rafał Wiench ◽  
Dariusz Skaba ◽  
Jacek Matys ◽  
Kinga Grzech-Leśniak
Keyword(s):  
Photodynamic Therapy ◽  
Toluidine Blue ◽  
Randomized Clinical Trials ◽  
Oral Candidiasis ◽  
In Vitro Studies ◽  
Cochrane Central Register ◽  
Antimicrobial Photodynamic Therapy ◽  
Candida Spp ◽  
Denture Stomatitis

The effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral yeast infections was examined many times in recent years. The authors of this review tried to address the question: “Should TBO (toluidine blue ortho)-mediated aPDT be considered a possible alternative treatment for oral candidiasis?”. PubMed/Medline and the Cochrane Central Register of Controlled Trials (CEN-TRAL) databases were searched from 1997 up to the 27th of October 2020 using a combination of the following keywords: (Candida OR Candidiasis oral OR Candidosis oral OR denture stomatitis) AND (toluidine blue OR photodynamic therapy OR aPDT OR photodynamic antimicrobial chemotherapy OR PACT OR photodynamic inactivation OR PDI). Animal studies or in vitro studies involving Candida albicans (C. albicans) and/or nonalbicans stain, randomized clinical trials (RCT) involving patients with oral candidiasis or denture stomatitis published solely in English language were included. Candida elimination method in animal, in vitro studies and RCT used was TBO-mediated aPDT. Exactly 393 studies were taken into consideration. Then, after analyzing titles and abstracts of said studies, 361 were excluded. Only 32 studies ended up being selected for in-depth screening, after which 21 of them were included in this study. All studies reported the antifungal effectiveness of aPDT with TBO against C. albicans and non-albicans Candida. In studies conducted with planktonic cells, only one study showed eradication of C. albicans. All others showed partial elimination and only one of them was not statistically significant. Experiments on yeast biofilms, in all cases, showed partial, statistically significant cell growth inhibition and weight reduction (a reduction in the number of cells—mainly hyphae) and the mass of extracellular polymeric substance (EPS). In vivo aPDT mediated by TBO exhibits antifungal effects against oral Candida spp.; however, its clinical effectiveness as a potent therapeutic strategy for oral yeast infections requires further investigation.

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