P096 LIPOPOLYSACCHARIDE-BINDING SYSTEMS IN PATIENTS WITH SARS-COV-19 INFECTION HAVING VARIOUS DEGREES OF SEVERITY

Refraction of the eye means its ability to bend (refract) light in its own optical system. In a normal state, which is called emmetropia, light rays passing through the optical system of the eye focus on the retina, from where the impulse is transmitted to the visual cortex of the brain and is analyzed there. A person sees equally well both in the distance and near in this situation. However, very often, refractive errors develop as a result of various types of influences. Myopia, or short-sightedness, occurs when the light rays are focused in front of the retina as a result of passing through the optical system of the eye. In this case, a person will clearly distinguish close objects and have difficulties in seeing distant objects. On the opposite side is development of farsightedness (hypermetropia), in which the focusing of light rays occurs behind the retina — such a person sees distant objects clearly, but outlines of closer objects are out of focus. Near vision impairment in old age is a natural process called presbyopia, it develops due to the lens thickening. Both myopia and hypermetropia can have different degrees of severity. The variant, when different refractive errors are observed in different eyes, is called anisometropia. In the same case, if different types of refraction are observed in the same eye, it is astigmatism, and most often it is a congenital pathology. Almost all of the above mentioned refractive errors require correction with spectacles or use of contact lenses. Recently, people are increasingly resorting to the methods of surgical vision correction.

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Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98841-1 ◽

1991 ◽

Vol 266 (20) ◽

pp. 13318-13323

Author(s):

T. Jomori ◽

S. Natori

Keyword(s):

Molecular Cloning ◽

Acute Phase ◽

Periplaneta Americana ◽

Binding Protein ◽

American Cockroach ◽

Lipopolysaccharide Binding Protein ◽

Lipopolysaccharide Binding

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Higher Lipopolysaccharide Binding Protein and Chemerin Concentrations Were Associated with Metabolic Syndrome Features in Pediatric Subjects with Abdominal Obesity during a Lifestyle Intervention

Nutrients ◽

10.3390/nu13020289 ◽

2021 ◽

Vol 13 (2) ◽

pp. 289

Author(s):

Amelia Marti ◽

Isabel Martínez ◽

Ana Ojeda-Rodríguez ◽

María Cristina Azcona-Sanjulian

Keyword(s):

Metabolic Syndrome ◽

Children And Adolescents ◽

Lifestyle Intervention ◽

Abdominal Obesity ◽

Plasma Levels ◽

Intervention Program ◽

Binding Protein ◽

Controlled Trial ◽

Lipopolysaccharide Binding Protein ◽

Lipopolysaccharide Binding

Background: Elevated circulating plasma levels of both lipopolysaccharide-binding protein (LBP) and chemerin are reported in patients with obesity, but few studies are available on lifestyle intervention programs. We investigated the association of both LBP and chemerin plasma levels with metabolic syndrome (MetS) outcomes in a lifestyle intervention in children and adolescents with abdominal obesity Methods: Twenty-nine patients enrolled in a randomized controlled trial were selected. The lifestyle intervention with a 2-month intensive phase and a subsequent 10-month follow-up consisted of a moderate calorie-restricted diet, recommendations to increase physical activity levels, and nutritional education. Results: Weight loss was accompanied by a significant reduction in MetS prevalence (−43%; p = 0.009). Chemerin (p = 0.029) and LBP (p = 0.033) plasma levels were significantly reduced at 2 months and 12 months, respectively. At the end of intervention, MetS components were associated with both LBP (p = 0.017) and chemerin (p < 0.001) plasma levels. Conclusions: We describe for the first time a reduction in both LBP and chemerin plasma levels and its association with MetS risk factors after a lifestyle intervention program in children and adolescents with abdominal obesity. Therefore, LBP and chemerin plasma levels could be used as biomarkers for the progression of cardiovascular risk in pediatric populations.

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State-of-the-art surgery for ulcerative colitis

Langenbeck s Archives of Surgery ◽

10.1007/s00423-021-02295-6 ◽

2021 ◽

Author(s):

Shanglei Liu ◽

Samuel Eisenstein

Keyword(s):

Ulcerative Colitis ◽

Surgical Intervention ◽

State Of The Art ◽

Total Proctocolectomy ◽

First Line ◽

Anastomotic Complications ◽

Permanent Ileostomy ◽

Elective Setting ◽

Pouch Reconstruction ◽

Degrees Of Severity

AbstractUlcerative colitis (UC) is an autoimmune-mediated colitis which can present in varying degrees of severity and increases the individual’s risk of developing colon cancer. While first-line treatment for UC is medical management, surgical treatment may be necessary in up to 25–30% of patients. With an increasing armamentarium of biologic therapies, patients are presenting for surgery much later in their course, and careful understanding of the complex interplay of the disease, its management, and the patient’s overall health is necessary when considering he appropriate way in which to address their disease surgically. Surgery is generally a total proctocolectomy either with pelvic pouch reconstruction or permanent ileostomy; however, this may need to be spread across multiple procedures given the complexity of the surgery weighed against the overall state of the patient’s health. Minimally invasive surgery, employing either laparoscopic, robotic, or transanal laparoscopic approaches, is currently the preferred approach in the elective setting. There is also some emerging evidence that appendectomy may delay the progression of UC in some individuals. Those who treat these patients surgically must also be familiar with the numerous potential pitfalls of surgical intervention and have plans in place for managing problems such as pouchitis, cuffitis, and anastomotic complications.

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Unusual presentation of pulmonary blastomycosis complicated by amphotericin-induced refractory electrolyte abnormalities

Oxford Medical Case Reports ◽

10.1093/omcr/omaa093 ◽

2020 ◽

Vol 2020 (11) ◽

Author(s):

Pool Tobar ◽

Alejandro Sanchez-Nadales ◽

Elena Caldeira ◽

Peguy Saad

Keyword(s):

Amphotericin B ◽

Liposomal Amphotericin ◽

Pulmonary Infection ◽

Pregnant Patient ◽

Lung Mass ◽

Radiographic Findings ◽

Common Presentation ◽

The Usa ◽

Degrees Of Severity ◽

Electrolyte Abnormalities

ABSTRACT Blastomyces dermatitidis is an endemic mold infection commonly seen in the midwestern of the USA and rarely affects pregnant women. The most common presentation is a pulmonary infection with variable degrees of severity. Of note, the clinical and radiographic findings of pulmonary blastomycosis can be mistaken for other pulmonary pathologies, thus earning the title of ‘the great pretender’. The treatment of choice of infected pregnant patients is amphotericin B, which is known to produce electrolyte imbalances. However, it infrequently causes severe, prolonged and profound hypokalemia and hypomagnesemia. We present the case of a 27-year-old pregnant patient with pulmonary blastomycosis presenting with a lung mass suspicious of malignancy and treated with amphotericin B with subsequent prolonged refractory hypokalemia and hypomagnesemia. Keywords pulmonary blastomycosis pregnancy biopsy computed tomography angiography liposomal amphotericin B

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Microbiome-Metabolomics Reveals Prebiotic Benefits of Fucoidan Supplementation in Mice

Journal of Marine Science and Engineering ◽

10.3390/jmse9050505 ◽

2021 ◽

Vol 9 (5) ◽

pp. 505

Author(s):

Jingyi Yuan ◽

Song Qin ◽

Wenjun Li ◽

Yubing Zhang ◽

Yuting Wang ◽

...

Keyword(s):

Lipid Metabolism ◽

Triglyceride Level ◽

Lactobacillus Reuteri ◽

Binding Protein ◽

Serum Triglyceride ◽

Serum Triglyceride Level ◽

Lipopolysaccharide Binding Protein ◽

Lactobacillus Gasseri ◽

Lactobacillus Animalis ◽

Lipopolysaccharide Binding

Fucoidan is a kind of polysaccharide with antitumor and antioxidant properties, which is mainly isolated from brown algae. Although there are many reports about the prebiotic effects of polysaccharides on hosts, there are few reports about the effects of fucoidan on blood biochemical indexes, intestinal microbiome, and metabolic function on healthy hosts. We applied 16S rRNA gene amplicon sequencing and LC-MS/MS metabolomics to evaluate the changes in the gut microbiome and metabolite profiles of fucoidan treatment in mice over 10 weeks. Fucoidan treatment modulated lipid metabolism, including significantly decreasing serum triglyceride level in healthy mice. Fucoidan also significantly inhibited serum lipopolysaccharide-binding protein (LBP) concentration, a biomarker of endotoxemia. Correlation analysis further showed that Lactobacillus animalis populations that were enriched by fucoidan demonstrated significantly negative correlations with serum triglyceride level. The abundance of Lactobacillus gasseri and Lactobacillus reuteri, increased by fucoidan supplementation, demonstrated significantly negative correlation with lipopolysaccharide-binding protein levels. Lactobacillus gasseri also demonstrated significantly positive correlations with three tryptophan-related metabolites, including indoleacrylic acid, 3-indoleacrylic acid, and 5-hydroxytryptamine, which were all increased by fucoidan administration. Combined with the previous evidence, the results indicate that fucoidan exerts prebiotic effects, such as lipid metabolism suppression and metabolic endotoxemia suppression, by modulating the abundance of gut microbiota, such as Lactobacillus animalis, Lactobacillus gasseri, and Lactobacillus reuteri, as well as microbiota-dependent metabolites, such as tryptophan-related metabolites.

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Gene variants of the bactericidal/permeability increasing protein and lipopolysaccharide binding protein in sepsis patients

Critical Care Medicine ◽

10.1097/00003246-200103000-00015 ◽

2001 ◽

Vol 29 (3) ◽

pp. 557-561 ◽

Cited By ~ 140

Author(s):

Jaroslav A. Hubacek ◽

Frank Stüber ◽

Dieter Fröhlich ◽

Malte Book ◽

Silke Wetegrove ◽

...

Keyword(s):

Binding Protein ◽

Gene Variants ◽

Lipopolysaccharide Binding Protein ◽

Lipopolysaccharide Binding

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Validation and calibration of the patient health questionnaire (PHQ-9) in Argentina

BMC Psychiatry ◽

10.1186/s12888-019-2262-9 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 13

Author(s):

María Urtasun ◽

Federico Manuel Daray ◽

Germán Leandro Teti ◽

Fernando Coppolillo ◽

Gabriela Herlax ◽

...

Keyword(s):

Depressive Symptoms ◽

Convergent Validity ◽

Patient Health Questionnaire ◽

Receiver Operating Curve ◽

Local Version ◽

Health Questionnaire ◽

Good Ability ◽

Patient Health ◽

Severity Of Depression ◽

Degrees Of Severity

Abstract Background The Patient Health Questionnaire-9 (PHQ-9) is a brief tool to assess the presence and severity of depressive symptoms. This study aimed to validate and calibrate the PHQ-9 to determine appropriate cut-off points for different degrees of severity of depression in Argentina. Methods We conducted a cross-sectional study on an intentional sample of adult ambulatory care patients with different degrees of severity of depression. All patients who completed the PHQ-9 were further interviewed by a trained clinician with the Mini International Neuropsychiatric Interview (MINI) and the Beck Depression Inventory-II (BDI-II). Reliability and validity tests, including receiver operating curve analysis, were performed. Results One hundred sixty-nine patients were recruited with a mean age of 47.4 years (SD = 14.8), of whom 102 were females (60.4%). The local PHQ-9 had high internal consistency (Cronbach’s alpha = 0.87) and satisfactory convergent validity with the BDI-II scale [Pearson’s correlation = 0.88 (p < 0.01)]. For the diagnosis of Major Depressive Episode (MDE) according to the MINI, a PHQ-9 ≥ 8 was the optimal cut-off point found (sensitivity 88.2%, specificity 86.6%, PPV 90.91%). The local version of PHQ-9 showed good ability to discriminate among depression severity categories according to the BDI-II scale. The best cut off points were 6–8 for mild cases, 9–14 for moderate and 15 or more for severe depressive symptoms respectively. Conclusions The Argentine version of the PHQ-9 questionnaire has shown acceptable validity and reliability for both screening and severity assessment of depressive symptoms.

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