Impact of simulated field drying on in vitro gas production and voluntary dry matter intake of rice straw

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.

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Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽

10.3390/ani10111957 ◽

2020 ◽

Vol 10 (11) ◽

pp. 1957

Author(s):

Margarita Novoa-Garrido ◽

Carlos Navarro Marcos ◽

María Dolores Carro Travieso ◽

Eduarda Molina Alcaide ◽

Mogens Larsen ◽

...

Keyword(s):

Dry Matter ◽

Nutritional Value ◽

Volatile Fatty Acids ◽

Gas Production ◽

Saccharina Latissima ◽

Porphyra Umbilicalis ◽

In Vitro Gas Production ◽

Ruminal Degradability ◽

Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

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Condensed tannins extracted from Quebracho affect the fermentation profile, nutritional quality and in vitro gas production of pornunça (Manihot spp.) silages

The Journal of Agricultural Science ◽

10.1017/s0021859621000605 ◽

2021 ◽

pp. 1-10

Author(s):

D. T. Q. Carvalho ◽

A. R. F. Lucena ◽

T. V. C. Nascimento ◽

L. M. L. Moura ◽

P. D. R. Marcelino ◽

...

Keyword(s):

Nutritional Quality ◽

Dry Matter ◽

Condensed Tannin ◽

Gas Production ◽

Rapid Decline ◽

Randomized Design ◽

In Vitro Gas Production ◽

Acid Detergent Fibre ◽

Fermentation Profile

Abstract The objective was to evaluate the fermentation profile, in vitro gas production and nutritional quality of pornunça (Manihot spp.) silages containing levels of condensed tannin (CT; 0, 4, 8 and 12% on dry matter (DM) basis), at five opening times (0, 3, 7, 14, 28 and 56 days). A completely randomized design in a 4 × 5 factorial arrangement was adopted, with four replications, totalling 80 experimental silos. The pH and NH3-N analyses were performed at all opening times of the silos. The other analyses were performed only with silages opened at 56 days of storage. There was an interaction effect between CT levels and silo opening times for pH and NH3-N. Tannin levels in pornunça silages after 56 days ensiling increased the pH and DM and reduced crude protein (CP) and neutral detergent fibre (NDF). There was a quadratic effect for NH3-N, acetic acid, butyric acid, gas losses, dry matter recovery (DMR), hemicellulose and acid detergent fibre. Inclusion of 4 and 8% CT in pornunça silage promotes a rapid decline in pH, being within the acceptable limit for adequate fermentation at 3 days of ensiling. Silages with 4% CT establish the pH at 28 days of opening the silos, with reduced NH3-N. Silages with 4% CT present higher concentrations of acetic and butyric acids and greater DMR. Inclusion of CT in pornunça silage after 56 days ensiling increases DM and reduces CP and NDF, directly affecting the in vitro degradability and reducing gas production.

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The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

Journal of Applied Animal Nutrition ◽

10.3920/jaan2021.0006 ◽

2021 ◽

pp. 1-6

Author(s):

N.D. Meads ◽

R. Tahmasbi ◽

N. Jantasila

Keyword(s):

Dry Matter ◽

Volatile Fatty Acids ◽

Environmental Science ◽

Ghg Emissions ◽

Negative Relationship ◽

Real Data ◽

Gas Production ◽

Production Technique ◽

In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

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Comparison of In situ Dry Matter Degradation with In vitro Gas Production of Oak Leaves Supplemented with or without Polyethylene Glycol (PEG)

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2006.1120 ◽

2006 ◽

Vol 19 (8) ◽

pp. 1120-1126 ◽

Cited By ~ 2

Author(s):

C. O. Ozkan ◽

M. Sahin

Keyword(s):

Polyethylene Glycol ◽

Dry Matter ◽

Gas Production ◽

In Vitro Gas Production ◽

Dry Matter Degradation ◽

Oak Leaves

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Comparison of parameters from the Theodorou gas production technique using nitrogen-free and nitrogen-rich media as predictors of dry-matter intake and digestibility

BSAP Occasional Publication ◽

10.1017/s0263967x00032493 ◽

1998 ◽

Vol 22 ◽

pp. 172-174

Author(s):

D. L. Romney ◽

F. C. Cadario ◽

E. Owen ◽

A .H. Murray

Keyword(s):

Dry Matter ◽

Gas Production ◽

Dry Matter Intake ◽

Production Technique ◽

N Supply ◽

Fermentation Method ◽

Rich Media ◽

Production Techniques

Parameters from in vitro gas production techniques could have potential as predictors of dry-matter intake (DMI) and digestibility. Fermentation is usually carried out under conditions where nitrogen (N) is not limiting. Therefore where N supply is a constraint to intake and digestibility, prediction equations may be inaccurate. This study compared the use of N-free and N-rich media in an in vitro fermentation method (Theodorou et al., 1994) and studied the relationships between in vitro and in vivo parameters obtained using both media.

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