scholarly journals Effects of dietary N-carbamylglutamate supplementation on milk production performance, nutrient digestibility and blood metabolomics of lactating Holstein cows under heat stress

Author(s):  
Ning Ma ◽  
Yan Li ◽  
Liyuan Ren ◽  
Linqi Hu ◽  
Ruiting Xu ◽  
...  
2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Gilson Sebastião Dias Júnior ◽  
Vítor Augusto Silveira ◽  
Ivan Júnior Ascari ◽  
Renata Apocalypse Nogueira Pereira ◽  
Marina de Arruda Camargo Danés ◽  
...  

ABSTRACT: The aim of this study was to evaluate the effect of total replacement of raw whole soybean (RAW) for roastedwhole soybean (ROS) on the production performance of Holstein cows. Two experiments were carried out usinga simple reversal design where RAW has been completely replaced by ROS. In experiment 1, 22 cows (175±60 days in milk)were used, and the dietary inclusion level of RAW or ROS was 3.7% of dry matter (DM). In experiment 2, 16 cows (130±50 days in milk)were used, and thedietary inclusion level of RAW or ROS was 11% of DM. In both experiments, ROS increased milk production by 1.1kgday-1 without changing fat and protein production. Dry matter intake or milk urea nitrogenwere not affected by dietary soy source. In experiment 2, plasma glucose concentration was decreased, and allantoin/creatinine ratio in urine tended to decreasein ROS. Experiment 2 also evaluated the nutrient digestibility and ruminal degradation kinetics of crude protein in two soybean sources. Roasting had no effect on the digestibility of DM, organic matter, and neutral detergent fiber. Roasted whole soybean hadgreater fraction B and lower protein degradation rate than did RAW; this showed that heat treatment was effective in increasing therumen undegradable amino acid flowto the animal, which suggesteda potential mechanism of action for improved performance observed in ROS.


Author(s):  
H. Mirzaei-Alamouti ◽  
A. Mohammad ◽  
M. Vazirigohar ◽  
P. Rezamand ◽  
M. Mansouryar

Abstract This study investigated whether the interaction of protein level and grain type can affect milk production, nutrient digestibility and rumen fermentation in primiparous Holstein cows. Four dietary treatments were used: high-protein with barley as the only grain source, HP-B; (2) high-protein with an equal mix of barley and maize, HP-BM; (3) low-protein with barley as the only grain source, LP-B and (4) low-protein with equal proportions of barley and maize, LP-BM. High-protein diets showed no improvement in milk or protein yield compared with low-protein, but barley and maize mix diets increased energy-corrected milk yield and fat yield compared with barley-only diets. The highest total apparent digestibility of dry matter, organic matter and neutral detergent fibre was observed for LP-BM whereas HP-BM showed the greatest crude protein digestibility. Treatment had no effect on total volatile fatty acid concentrations, molar proportion of acetate and propionate and acetate to propionate ratio. The lowest ruminal pH was observed for LP-B. High-protein diets resulted in greater concentrations of ammonia nitrogen (N), urinary N, blood and milk urea nitrogen compared with low-protein diets, whereas low-protein diets showed better nitrogen utilization efficiency. This study showed that primiparous lactating cows do not benefit from high-protein diets with different fermentation rates of grain sources, but barley and maize diets may improve milk production performance, ruminal fermentation and pH under the present dietary conditions. The current results on milk production performance should be interpreted with caution due to the small number of cows used (eight in each treatment).


2020 ◽  
Author(s):  
Ning Ma ◽  
Yan Li ◽  
Liyuan Ren ◽  
Linqi Hu ◽  
Ruiting Xu ◽  
...  

Abstract Background: Heat stress (HS) becomes a serious threat to dairy cow industry in certain circumstances, N-carbamylglutamate (NCG) is a novel feed additive that may be used to alleviate HS. However, the effects and mechanisms of NCG on dairy cows under HS are still unknown. A total of 48 Holstein dairy cows with similar days in milk (154.37±13.56 d), parity (1-3), and body condition score (BCS) were randomly divided into 4 groups of 12 animals each. Under HS, the cows were fed a TMR diet supplemented with 0 (control), 15, 20, 25 g of NCG/d per cow for 60 days. Milk production performance was recorded and serum parameters were examined. Meanwhile, metabolomics study of plasma based on liquid chromatography–mass spectrometry (LC-MS) was also applied to investigate metabolites and pathways in response to NCG supplementation.Results: Milk yield (MY) was increased but milk urine nitrogen (MUN) was reduced by the NCG treatment. Addition of 15 g of NCG/d increased the milk protein percentage (MPP) compared with the control. No effect of NCG was observed on dry matter intake (DMI), lactose percentage (LP), milk fat percentage (MFP) and somatic cell count (SCC). Serum glucose (GLU) levels in cows fed with 15, 20, 25 g of NCG/d were increased by 14.35%, 19.34% and 18.63% in comparison with the control. Meanwhile, the concentrations of blood urea nitrogen (BUN) and blood ammonia (BA) were decreased with the addition of NCG. Results for antioxidant capacity and immune function showed beneficial effects of NCG, such as the increases in total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IgG, IgM and IgA. Clear separations of plasma metabolic profiles between control and NCG groups were observed in the score plots. Sixteen different metabolites involved in metabolisms of amino acids, ketone bodies, butanoate and energy, as well as gut microbiome-derived metabolism were regulated by NCG supplementation.Conclusions: This study found that NCG treatment improved antioxidant capacity, immune function, production performance, and metabolic profile of dairy cows under HS and provided new evidence for the better utilization of NCG.


2022 ◽  
Vol 8 ◽  
Author(s):  
Yizhe Cui ◽  
Zhuorui Shan ◽  
Lintong Hou ◽  
Qiuju Wang ◽  
Juan J. Loor ◽  
...  

This study examined the effect of mixed medicinal herbs from China in the ground form on milk yield and various blood metabolites before and after parturition in Holstein cows. Crushed Agastache rugosus, Scutellaria barbata, Pericarpium citri reticulate, and Radix glycyrrhizae were used to develop TCMF4. Thirty-two Chinese Holstein cows were randomly divided into a control group or groups receiving 0.1, 0.3, or 0.5 kg TCMF4/cow/d from −7 through 21 d relative to parturition. Blood samples for serum isolation were collected at −7, −1, 1, 7, 14, and 21 d relative to parturition and used to measure glucose, β-hydroxybutyric acid (BHBA), total protein, albumin, globulin, and alkaline phosphatase. Milk production was recorded daily for the first 21 d postpartum, and composition was analyzed at 7, 14, and 21 d. Data were analyzed using a one-way analysis of variance (ANOVA) for multiple comparisons. The average milk production during the first 21-d postpartum was 28.7 ± 6.9, 27.2 ± 7.1, 31.2 ± 6.8, and 38.5 ± 6.1 kg/d for control group and groups receiving 0.1, 0.3, or 0.5 kg TCMF4. Thus, average daily milk production increased between 9 to 34% by supplementation with TCMF4 compared with the control group. Compared with the control group, in the middle dose group, milk concentrations of lactose and total protein decreased by 21 and 19%, respectively, at d 7 around parturition, while total solids increased by 23% at d 21 in the high-dose group. Furthermore, compared with the control group, serum BHBA decreased by 50 and 20% at d −1 and 21 around parturition in the high-dose group. Overall, TCMF4 supplementation improved dry matter intake (DMI) and milk production of dairy cows during the periparturient period without adverse effects on liver function, and plasma BHBA concentrations of dairy cows tended to decrease when dietary TCMF4 increased, which suggested that TCMF4 might be used as potential additives in dairy cows to improve production performance.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 389-389
Author(s):  
Andrea Dahnae del Rio-Aviles ◽  
Abelardo Correa-Calderon ◽  
Leonel Avendaño-Reyes ◽  
Milton G Thomas ◽  
R Mark Enns ◽  
...  

Abstract Summer season in northwest Mexico is characterized by hot-humid weather conditions that lead to heat stress and affect physiological and productive performance of lactating Holstein cows. Mineral supplementation has been proposed as strategy to alleviate negative effects of heat stress in dairy cattle. Thus, the objective of this study was to evaluate the effect of an injectable mineral supplement on physiological variables and milk production in Holstein cows managed during summer. Sixteen cows were randomly assigned to 1 of 2 experimental groups: Mineral-treated (TRT; n = 8) or control (CON; n = 8). The TRT group received 3 injections of Fosfosan® (10 ml, i.m., Virbac, Uruguay), on days 0, 7 and 17, considering summer onset as day 0 (June 21st), whereas group CON was untreated. Physiological variables such as respiratory rate (RR), vaginal temperature (VT) and skin-surface temperature (SST), were registered twice a week (0500-1700 h) during 6 weeks. Ambient temperature and humidity were collected to calculate the daily temperature and humidity index (THI). Data were analyzed using a mixed model with repeated measures, which included fixed effects of treatment, time and treatment*time, and sire as random effect. According to the THI, Holstein cows were exposed to heat stress during the experiment (>72 units). Cows in the TRT group showed higher (P < 0.05) milk production than CON cows (17.93±0.47 vs 17.02±0.51 kg/day) and lower (P < 0.01) VT (39.14±0.11 vs 39.46±0.15 °C). Relative to CON cows, treated cows also had lower RR (63.16±0.65 vs 64.47±0.76 breaths/min) and lower SST (33.75±0.15 vs 34.02±0.16 °C). All physiological variables showed a significant effect of time (P < 0.01). In conclusion, mineral supplementation during summer appears to improve physiological and productive performance in heat-stressed milking dairy cows; therefore, we suggest to consider this management as strategy to reduce negative effects of summer and heat stress on the dairy industry in northwest Mexico.


2016 ◽  
pp. 15-19
Author(s):  
Michal Vlček ◽  
Radovan Kasarda

The aim of the study was the influence of claw disorders on production traits in dairy cattle. Observed were claw traits as claw angle, claw length, heel index, claw height, claw diagonal and claw width. Right hind claw after functional trimming was evaluated. Occurrence of claw disorders like interdigital dermatitis and heel erosion (IDHE), digital dermatitis (DD) and sole ulcer (SV) as well as the progress of disease were observed. Basic summary and variation statistics was performed by the SAS software. Two herds were included in the study. Holstein dairy cows (n=101) produced 11 875 kg of milk, 468 kg (3.94%) of fat and 396 kg (3.34%) of proteins. Holstein cows were affected with IDHE in 22 cases, with DD in 6 cases and with sole ulcer in 10 cases. Slovak Simmental dairy cows (n=101) produced 5834 kg of milk, 258 kg (4.44%) of fat and 209 kg (3.59%) of proteins. Slovak Simmental cows were affected with IDHE in 8 cases, with DD in 5 cases and with sole ulcer in 19 cases. Observed was that claw disorders caused deviations of milk production.


Author(s):  
B. N. Thorat ◽  
B. M. Thombre ◽  
S. G. Narwade

Improvement in production performance of indigenous cattle breeds becomes essential to make them economically viable. Due to increased environmental temperature cattle undergo heat stress. Milk production is adversely affected due to heat stress. In the present study records maintained at Cattle Cross Breeding Project, Vasantrao Naik Marathawada Krishi Vidyapeeth, Parbhani (Maharashtra) for the period of 1995-2010 were utilized to study monthly lactation milk yields and climatic factors. The climatic parameters considered were temperature, relative humidity, wind velocity, sunshine hours and dry and wet bulb temperature along with THI. The data on monthly lactation milk yields of cows calved during cold, hot, south-west monsoon and post monsoon seasons were considered. Correlation and multiple regression analysis were used to investigate various sources of variation in monthly lactation milk yields. Monthly lactation milk yield data were analyzed to ascertain the effect of climatic attributes and to know their association with monthly lactation milk yields. The higher milk production during post monsoon was attributed to cows calved during that season whose milk production was favoured by post monsoon climate. Seasonal climate may be the responsible factor to influence the milk production levels. The average monthly milk production (359.18+25.85 liter) was more during post monsoon season followed by cold (326.81+20.48 liter), south-west monsoon seasons (326.60 +20.45 liter) and hot (245.78+19.13 liter), respectively.


2018 ◽  
Vol 29 (1) ◽  
pp. 241-249
Author(s):  
SeokHyun Lee ◽  
Yunho Choy ◽  
Chang-Gwon Dang ◽  
Alam Mahboob ◽  
Kwanghyun Cho

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