Strategies for controlling inbreeding in fish breeding programs; an applied approach using optimum contribution (OC) procedures

Pembentukan ikan mas transgenik merupakan salah satu program penelitian di Balai Penelitian Pemuliaan Ikan, Sukamandi dalam rangka menghasilkan varietas unggul ikan mas tahan infeksi KHV (Koi herpesvirus). Pada tahun 2015 telah dilakukan pembentukan ikan mas transgenik tahan KHV generasi F-2. Penelitian ini bertujuan untuk mengevaluasi transmisi gen krt-GP11, ketahanan ikan mas transgenik F-2 terhadap infeksi KHV, keberadaan marka Cyca-DAB1*05 tahan KHV pada populasi ikan mas transgenik F-2. Ikan mas transgenik F-2 dihasilkan dengan memijahkan ikan mas transgenik F-1 jantan dengan betina non-transgenik. Pengujian transmisi transgen dan deteksi marka ketahanan KHV pada transgenik F-2 dilakukan dengan metode PCR menggunakan primer spesifik untuk transgen krt-GP11 dan gen Cyca-DAB1*05. Evaluasi ketahanan ikan mas transgenik F-2 terhadap infeksi KHV dilakukan dengan uji tantang secara kohabitasi. Hasil penelitian menunjukkan bahwa transmisi gen krt-GP11 pada keturunan F-2 memiliki persentase yang relatif rendah yaitu sebesar 0%-2%. Ikan mas transgenik F-2 memiliki ketahanan relatif baik terhadap KHV dengan sintasan uji tantang sebesar 90% dan tidak berbeda nyata dengan ikan mas pembanding atau non-transgenik (P>0,05). Tingginya pesentase keberadaan marka Cyca-DAB1*05 pada populasi transgenik berperan pada ketahanan ikan mas transgenik terhadap infeksi KHV.Creating of transgenic common carp is one of the breeding programs in Research Institute for Fish Breeding for producing a superior strain of common carp resistant to KHV(Koi herpesvirus). Since 2015, the creation of common carp transgenic has been conducted to produce F2 population resistant to KHV. This study was aimed to evaluate the transmission of krt-GP11 gen,the resistantce of F2 transgenic common carp against to KHV infection, and the existence of Cyca-DAB1*05 marker resistant to KHV in F2 transgenic population. F2 transgenic population has been produced by mating F1 transgenic male with non transgenic female. Transgene transmission and the existence of marker resistant to KHV in F2 transgenic population were evaluated by PCR method using specific primer to krt-GP11 gene and Cyca-DAB1*05 gene, respectively. The resistance of F2 transgenic population againstto KHV infection was evaluated by challenge test using cohabitation method. The result showed that transmission of krt-GP11 gene in F2transgenic population was relatively low with percentage of 0-2%. The resistance of F2 transgenic common carp against to KHV was relatively high with survival rate of 90% and was not significantly different from non transgenic (p>0.05). High percentage of transgenic population having Cyca-DAB1*05 marker had a role in resistance of transgenic population against KHV infection.

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Growth curve of Nile tilapia from different families of the AquaAmérica variety

Brazilian Journal of Biology ◽

10.1590/1519-6984.243534 ◽

2022 ◽

Vol 82 ◽

Author(s):

J. C. Carvalho ◽

R. A. C. Corrêa Filho ◽

C. A. L. Oliveira ◽

R. P. Ribeiro ◽

G. N. Seraphim ◽

...

Keyword(s):

Growth Curve ◽

Nile Tilapia ◽

Specific Growth ◽

Least Squares Method ◽

Weighted Least Squares ◽

Breeding Programs ◽

Weighted Least Squares Method ◽

Fish Breeding ◽

Asymptotic Values ◽

Abstract Selection

Abstract Selection can affect growth, changing performance and asymptotic values. However, there is little information about the growth of families in fish breeding programs. The aim of this study was to evaluate the performance and growth of families of Nile tilapia AquaAmérica. Twenty AquaAmérica families cultivated in a net cage (13.5 m3) for 181 days were evaluated. The nonlinear Gompertz regression model was fitted to the data by the weighted least squares method, taking the inverse of the variance of weight in different families and at different ages as the weighting variable. The model was adjusted to describe the growth in weight and morphometric characteristics. Two families showed highest (P<0.05) weights at both 133 days (family AA10: 743.2 g; family AA16: 741.2 g) and 181 days (family AA10: 1,422.1 g; family AA16: 1,393.4 g) of the experiment. In both experimental periods, the males showed a heavier weight, with the greatest contrast between the sexes occurring at 181 days. The analysis of the three most contrasting families (AA1, AA9 and AA14) showed that the asymptotic value for weight was higher (P<0.05) in family AA9 (3,926.3 g) than in family AA14 (3,251.6 g), but specific growth rate and age at the inflection point did not differ significantly between families. In conclusion, two of the 20 families were superior; males exhibited a greater growth, mainly in the period of 181 days; and the growth curve differed between the families, especially for asymptotic weight.

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Genetic variability in Tambaqui generations of the breeding program in the Central-West Region of Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n3supl1p1785 ◽

2021 ◽

Vol 42 (3Supl1) ◽

pp. 1785-1796

Author(s):

Angela Maria Urrea-Rojas ◽

◽

Annaiza Braga Bignardi ◽

Felipe Pinheiro de Souza ◽

Ed Christian Suzuki de Lima ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Native Species ◽

Genetic Improvement ◽

Improvement Program ◽

Breeding Programs ◽

Mato Grosso ◽

West Region ◽

Fish Breeding ◽

The Mean

The implementation of fish breeding programs in Brazil has brought significant results in the productivity of tilapia. However, the insertion of native species with great potential (such as Tambaqui Colossoma macropomum) in these programs is still recent, and thus requires genetic information for monitoring and enabling their consolidation into the programs. The objective of the present study was to evaluate the genetic diversity of the parental generation (G0) and two consecutive generations (G1 and G2) in the C. macropomum genetic improvement program, located in the municipality of Sorriso, Mato Grosso, Brazil. Ninety caudal fin samples were collected (30 samples per generation) for DNA extraction. The genetic study implemented seven microsatellite markers (Cm1A8, Cm1A11, Cm1D1, Cm1E3, Cm1F4, Cm1F5, and Cm1H8). A total of 17 alleles were amplified, with variations in the mean number between four to two alleles per locus. The size per locus ranged from 170 to 360 bp. The average inbreeding coefficient was 0.126 (G0), -0.040 (G1), and 0.131 (G2). No null or exclusive alleles were found. The observed heterozygosity values for G1 and G2 demonstrated the preservation of genetic variability (0.453 and 0.409, respectively). In conclusion, the genetic diversity of the parental generation (G0) and the two progenies generations (G1 and G2) were adequate, which demonstrates that the genetic improvement program was conducted correctly; however, it is important to continue to evaluations the genetic diversity of the future progeny.

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ESTIMASI NILAI HERITABILITAS BOBOT IKAN MAS VARIETAS PUNTEN DALAM PROGRAM SELEKSI INDIVIDU

Jurnal Riset Akuakultur ◽

10.15578/jra.11.3.2016.217-223 ◽

2016 ◽

Vol 11 (3) ◽

pp. 217

Author(s):

Estu Nugroho ◽

Budi Setyono ◽

Mochammad Su’eb ◽

Tri Heru Prihadi

Keyword(s):

Body Weight ◽

Selection Response ◽

Base Population ◽

Breeding Programs ◽

Male And Female ◽

Body Weight Trait ◽

Population Selection ◽

Selection For ◽

Weight Trait ◽

Selection Of

Program pemuliaan ikan mas varietas Punten dilakukan dengan seleksi individu terhadap karakter bobot ikan. Pembentukan populasi dasar untuk kegiatan seleksi dilakukan dengan memijahkan secara massal induk ikan mas yang terdiri atas 20 induk betina dan 21 induk jantan yang dikoleksi dari daerah Punten, Kepanjen (delapan betina dan enam jantan), Kediri (tujuh betina dan 12 jantan), Sragen (27 betina dan 10 jantan), dan Blitar (15 betina dan 11 jantan). Larva umur 10 hari dipelihara selama empat bulan. Selanjutnya dilakukan penjarangan sebesar 50% dan benih dipelihara selama 14 bulan untuk dilakukan seleksi dengan panduan hasil sampling 250 ekor individu setiap populasi. Seleksi terhadap calon induk dilakukan saat umur 18 bulan pada populasi jantan dan betina secara terpisah dengan memilih berdasarkan 10% bobot ikan yang terbaik. Calon induk yang terseleksi kemudian dipelihara hingga matang gonad, kemudian dipilih sebanyak 150 pasang dan dipijahkan secara massal. Didapatkan respons positif dari hasil seleksi berdasarkan bobot ikan, yaitu 49,89 g atau 3,66% (populasi ikan jantan) dan 168,47 g atau 11,43% (populasi ikan betina). Nilai heritabilitas untuk bobot ikan adalah 0,238 (jantan) dan 0,505 (betina).Punten carp breeding programs were carried out by individual selection for body weight trait. The base population for selection activities were conducted by mass breeding of parent consisted of 20 female and 21 male collected from area Punten, eight female and six male (Kepanjen), seven female and 12 male (Kediri), 27 female and 10 male (Sragen), 15 female and 11 male (Blitar). Larvae 10 days old reared for four moths. Then after spacing out 50% of total harvest, the offspring reared for 14 months for selection activity based on the sampling of 250 individual each population. Selection of broodstock candidates performed since 18 months age on male and female populations separately by selecting based on 10% of fish with best body weight. Candidates selected broodstocks were then maintained until mature. In oder to produce the next generation 150 pairs were sets and held for mass spawning. The results revealed that selection response were positive, 49.89 g (3.66%) for male and 168.47 (11.43%) for female. Heritability for body weight is 0.238 (male) and 0.505 (female).

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SEEDING DENSITY OF DIFFERENT FISH SPECIES IN POLYCULTURE

VESTNIK OF ASTRAKHAN STATE TECHNICAL UNIVERSITY SERIES FISHING INDUSTRY ◽

10.24143/2073-5529-2017-3-74-79 ◽

2017 ◽

pp. 74-79

Author(s):

Yuriy Borisovich Lvov

Keyword(s):

Fish Species ◽

Silver Carp ◽

Seeding Density ◽

Habitat Conditions ◽

Method Of Calculation ◽

Fish Breeding ◽

Trophic Preferences ◽

Breeding Zone ◽

Practical Progress

The article touches upon the principles, biological basics and practical progress of introduction of specific and age polyculture of fishes. Using the results of the analysis of literary and long-term native data, there has been offered the gradation of 12 fish species from different families by resistance to habitat conditions. Crucian ( Carassius gibelio ) has been chosen as the most sustainable object of cultivation. There has been taken an attempt to calculate the number of seeding different fish species in polyculture taking into account their physiological features is made. Subject to specific physiological features of the studied species, there was carried out recalculation of their natural reproductive performance in different fish-breeding zones, which is traditionally carried out for a carp ( Cyprinus carpio ). In particular, in the second fish-breeding zone with area of 1 hectare it is possible to breed 120 kg of carp, or 150 kg of crucian, or 75 kg of silver carp ( Hypophthalmichthys ). Overall productivity is made up of a share of fishes in polyculture, but taking into account tolerance of species, it can be lower than productivity calculated for carp. The method of calculation and formulas which give the chance to determine seeding density of fish species for breeding in polyculture have been offered. This technique makes it possible to use trophic preferences and consider tolerance of each species to the conditions of breeding areas.

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A Comparative Analysis of Two Qualitative Methods: Deciding Between Grounded Theory and Phenomenology for Your Research

Vocational Training Research And Realities ◽

10.2478/vtrr-2017-0003 ◽

2017 ◽

Vol 28 (1) ◽

pp. 23-40

Author(s):

Masoodi Marjan

Keyword(s):

Grounded Theory ◽

Data Analysis ◽

Comparative Analysis ◽

Historical Development ◽

Research Question ◽

Qualitative Approaches ◽

Development Goals ◽

Applied Approach ◽

Similarities And Differences ◽

Research Students

Abstract The purpose of this article is to compare two qualitative approaches that can be used in different researches: phenomenology and grounded theory. This overview is done to (1) summarize similarities and differences between these two approaches, with attention to their historical development, goals, methods, audience, and products (2) familiarize the researchers with the origins and details of these approaches in the way that they can make better matches between their research question(s) and the goals and products of the study (3) discuss a brief outline of each methodology along with their origin, essence and procedural steps undertaken (4) illustrate how the procedures of data analysis (coding), theoretical memoing and sampling are applied to systematically generate a grounded theory (5) briefly examine the major challenges for utilizing two approaches in grounded theory, the Glaserian and Straussian. As a conclusion, this overview reveals that it is essential to ensure that the method matches the research question being asked, helps the researchers determine the suitability of their applied approach and provides a continues training for the novice researchers, especially PhD or research students who lack solid knowledge and background experience in multiple research methods.

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SCREENING OF WHEAT LR-GENES FOR RESISTANCE TO PUCCINIA TRITICINA IN THE NORTH CAUCASUS REGION CONDITIONS

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2019/5/54-56 ◽

1970 ◽

pp. 54-56

Author(s):

G. V. Volkova ◽

O. A. Kudinova ◽

O. F. Vaganova

Keyword(s):

Winter Wheat ◽

Leaf Rust ◽

Puccinia Triticina ◽

North Caucasus ◽

Breeding Programs ◽

Lr Genes ◽

Caucasus Region ◽

Highly Efficient ◽

The North ◽

Wheat Rust

Currently, more than 70 wheat rust resistance genes are known, but few of them are effective. The purpose of this work is to screen lines of Lr gene carriers for resistance to leaf rust under conditions of the North Caucasus region. Investigations were carried out in 2016-2018 at the infectious site of VNIIBZR. Research material was 49 near isogenic lines of winter wheat cultivar Thatcher. Infectious material was the combined populations of P. triticina, obtained as a result of route surveys of industrial and breeding crops of winter wheat in the areas of the Krasnodar, Stavropol Territories and the Rostov Region, conducted in 2016-2018. According to the assessment, the genes are ranked as follows: - highly efficient genes (plants with no signs of damage): Lr9, Lr42, Lr43 + 24 and Lr50; effective (1R-5R) Lr genes: 19, 24, 29, 36, 37, 38, 45, 47; moderately effective (10MR-20MR) Lr genes: 17, 18, 21, 22a, 28, 32, 41, 52. The remaining Lr-lines were susceptible to P. triticina (25 MR - 90S) to varying degrees. Highly efficient and effective genes Lr9, Lr19, Lr24, Lr29, Lr38, Lr42, Lr43 + 24, Lr47 and Lr50 showed resistance in the seedling phase and can be recommended for inclusion in breeding programs to protect wheat from leaf rust in different phases of plant ontogenesis in the North Caucasus region.

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Matoa (Pometia Pinata Foster): Local Fruit Papua and Strategy Development

Beccariana Botanical Research Bulletin ◽

10.30862/bbrp.v7i1.276 ◽

2010 ◽

Vol 7 (1) ◽

Author(s):

Julius D. Nugroho

Keyword(s):

Genetic Resources ◽

Fruit Tree ◽

Breeding Program ◽

Strategy Development ◽

Local Market ◽

Font Size ◽

Breeding Programs ◽

Irian Jaya ◽

Forest Exploitation ◽

Program Strategy

Matoa (Pometia pinnata) is a local fruit of  Papua (formerly called Irian Jaya) which has high potensial to develop as comercial fruit. Highly significant genetic resources of matoa potentially for breeding program in Papua are being threatened as a result of cutting down trees for fruit harvesting and of forest exploitation for timber. Besides the loss of genetic resources facing now, other major problems should be consider for conservation and domestication of this fruit tree species i.e. lack of silviculture and agronomy knowledge for further breeding programs; matoa production only for local market; and inadequate government policy for matoa breeding program. Strategy developed for matoa conservation and domestication should also concern about time limited due to the fast loss of genetic poll. This paper provides a general overview of strategy for conservation and domestication of Pometia pinnata with special reference to Papua.

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