Host life stage- and temperature-dependent density of the symbiont Buchnera aphidicola in a subtropical pea aphid (Acyrthosiphon pisum) population

AbstractA simple age-specific energetics (calories or biomass) model for the growth and development, reproduction, respiration, ageing, and intrinsic survivorship as a function of temperature and per capita energy availability for pea aphid (Acyrthosiphon pisum (Harris)) is reported. The ratio of energy supply–demand is used to scale all of the rates in the model. The maximum demand for energy based upon current state values is used to drive the Frazer–Gilbert functional response model (i.e. food acquisition), which is a component of the metabolic pool model used to assimilate energy to growth, reproduction, respiration, and egestion. The extensive data sets on pea aphid energetics published by Randolph et al. (1975) were used to develop the model. As the model estimates reproduction (Mx) and survivorship (Lx) values, extensive published age-specific life-data sets on pea aphids are used to test it. The results suggest:(1) the lower thermal threshold for development is raised and the upper threshold is lowered as food resources are decreased(2) the temperature-dependent rate of development is slowed with decreasing energy resources(3) the size of individuals and reproduction become smaller as temperature approaches the upper and lower thermal thresholds.A simple model for multitrophic level interactions incorporating the acquisition and assimilation functions is presented.

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A dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont, Buchnera aphidicola

BMC Genomics ◽

10.1186/1471-2164-7-50 ◽

2006 ◽

Vol 7 (1) ◽

Cited By ~ 49

Author(s):

Alex CC Wilson ◽

Helen E Dunbar ◽

Gregory K Davis ◽

Wayne B Hunter ◽

David L Stern ◽

...

Keyword(s):

Acyrthosiphon Pisum ◽

Bacterial Symbiont ◽

Pea Aphid ◽

Buchnera Aphidicola

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Impact of Host Developmental Age on the Transcriptome of the Symbiotic Bacterium Buchnera aphidicola in the Pea Aphid (Acyrthosiphon pisum)

Applied and Environmental Microbiology ◽

10.1128/aem.01472-09 ◽

2009 ◽

Vol 75 (22) ◽

pp. 7294-7297 ◽

Cited By ~ 21

Author(s):

John Bermingham ◽

Andrï¿½ane Rabatel ◽

Federica Calevro ◽

Josï¿½ Viï¿½uelas ◽

Gï¿½rard Febvay ◽

...

Keyword(s):

Acyrthosiphon Pisum ◽

Flagellar Apparatus ◽

Symbiotic Bacterium ◽

Bacterial Symbiont ◽

Pea Aphid ◽

Differentially Expressed ◽

Buchnera Aphidicola ◽

Functional Differences ◽

Developmental Age ◽

Riboflavin Synthesis

ABSTRACT Of the 617 genes from Buchnera aphidicola, the obligate bacterial symbiont of the pea aphid, 23% were differentially expressed in embryos compared to adults. Genes involved in flagellar apparatus and riboflavin synthesis exhibited particularly robust upregulation in embryos, suggesting functional differences between the symbiosis in the adult and embryo insect.

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Effects of Endosymbiont Disruption on the Nutritional Dynamics of the Pea Aphid Acyrthosiphon pisum

Insects ◽

10.3390/insects9040161 ◽

2018 ◽

Vol 9 (4) ◽

pp. 161 ◽

Cited By ~ 2

Author(s):

Ning Lv ◽

Lei Wang ◽

Wen Sang ◽

Chang-Zhong Liu ◽

Bao-Li Qiu

Keyword(s):

Energy Metabolism ◽

Host Plants ◽

Acyrthosiphon Pisum ◽

Soluble Sugars ◽

Pea Aphid ◽

Buchnera Aphidicola ◽

Phloem Sap ◽

Nutritional Factors ◽

Pea Aphids ◽

Aphid Host

Pea aphid (Acyrthosiphon pisum) is a worldwide pest that feeds exclusively on the phloem sap of numerous host plants. It harbours a well-known primary endosymbiont Buchnera aphidicola that helps to overcome the nutritional deficiency of a plant-based diet. However, how the Buchnera contributes to the nutritional and energy metabolism of its aphid host is unclear to date. In the current study, the function of Buchnera in relation to nutritional synthesis of pea aphid was investigated by disrupting the primary endosymbiont with an antibiotic rifampicin. Our findings revealed that the disruption of Buchnera led to infertility and higher loss in body mass of aphid hosts. Body length and width were also decreased significantly compared to healthy aphids. The detection of nutrition indicated that the quantity of proteins, soluble sugars, and glycogen in aposymbiotic pea aphids increased slowly with the growth of the aphid host. In comparison, the quantities of all the nutritional factors were significantly lower than those of symbiotic pea aphids, while the quantity of total lipid and neutral fat in aposymbiotic pea aphids were distinctly higher than those of symbiotic ones. Thus, we concluded that the significant reduction of the total amount of proteins, soluble sugars, and glycogen and the significant increase of neutral fats in aposymbiotic pea aphids were due to the disruption of Buchnera, which confirmed that the function of Buchnera is irreplaceable in the pea aphid.

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Genomic evidence for complementary purine metabolism in the pea aphid, Acyrthosiphon pisum, and its symbiotic bacterium Buchnera aphidicola

Insect Molecular Biology ◽

10.1111/j.1365-2583.2009.00945.x ◽

2010 ◽

Vol 19 ◽

pp. 241-248 ◽

Cited By ~ 32

Author(s):

J. S. Ramsey ◽

S. J. MacDonald ◽

G. Jander ◽

A. Nakabachi ◽

G. H. Thomas ◽

...

Keyword(s):

Acyrthosiphon Pisum ◽

Symbiotic Bacterium ◽

Purine Metabolism ◽

Pea Aphid ◽

Buchnera Aphidicola

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Multimodal dynamic response of the Buchnera aphidicola pLeu plasmid to variations in leucine demand of its host, the pea aphid Acyrthosiphon pisum

Molecular Microbiology ◽

10.1111/j.1365-2958.2011.07760.x ◽

2011 ◽

Vol 81 (5) ◽

pp. 1271-1285 ◽

Cited By ~ 15

Author(s):

José Viñuelas ◽

Gérard Febvay ◽

Gabrielle Duport ◽

Stefano Colella ◽

Jean-Michel Fayard ◽

...

Keyword(s):

Dynamic Response ◽

Acyrthosiphon Pisum ◽

Pea Aphid ◽

Buchnera Aphidicola

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Systemic analysis of the symbiotic function of Buchnera aphidicola, the primary endosymbiont of the pea aphid Acyrthosiphon pisum

Comptes Rendus Biologies ◽

10.1016/j.crvi.2009.09.007 ◽

2009 ◽

Vol 332 (11) ◽

pp. 1034-1049 ◽

Cited By ~ 32

Author(s):

Lilia Brinza ◽

José Viñuelas ◽

Ludovic Cottret ◽

Federica Calevro ◽

Yvan Rahbé ◽

...

Keyword(s):

Acyrthosiphon Pisum ◽

Pea Aphid ◽

Buchnera Aphidicola ◽

Systemic Analysis

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Role of Host Nutrition in Symbiont Regulation: Impact of Dietary Nitrogen on Proliferation of Obligate and Facultative Bacterial Endosymbionts of the Pea Aphid Acyrthosiphon pisum

Applied and Environmental Microbiology ◽

10.1128/aem.01211-06 ◽

2006 ◽

Vol 73 (4) ◽

pp. 1362-1366 ◽

Cited By ~ 43

Author(s):

T. L. Wilkinson ◽

R. Koga ◽

T. Fukatsu

Keyword(s):

Population Density ◽

Acyrthosiphon Pisum ◽

Pea Aphid ◽

Buchnera Aphidicola ◽

Nitrogen Levels ◽

Dietary Nitrogen ◽

Obligate Symbiont ◽

Host Nutrition ◽

Bacterial Endosymbionts ◽

The Impact

ABSTRACT The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increased in aphids reared on low-nitrogen diets, indicating distinct regulatory mechanisms in the same insect host.

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Trehalose and glucose levels regulate feeding behavior of the phloem-feeding insect, the pea aphid Acyrthosiphon pisum Harris

Scientific Reports ◽

10.1038/s41598-021-95390-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Guang Wang ◽

Jing-Jiang Zhou ◽

Yan Li ◽

Yuping Gou ◽

Peter Quandahor ◽

...

Keyword(s):

Feeding Behavior ◽

Growth And Development ◽

Acyrthosiphon Pisum ◽

Pea Aphid ◽

Feeding Time ◽

Electrical Penetration Graph ◽

Glucose Content ◽

Glucose Levels ◽

Phloem Feeding ◽

Trehalose Content

AbstractTrehalose serves multifarious roles in growth and development of insects. In this study, we demonstrated that the high trehalose diet increased the glucose content, and high glucose diet increased the glucose content but decreased the trehalose content of Acyrthosiphon pisum. RNA interference (RNAi) of trehalose-6-phosphate synthase gene (ApTPS) decreased while RNAi of trehalase gene (ApTRE) increased the trehalose and glucose contents. In the electrical penetration graph experiment, RNAi of ApTPS increased the percentage of E2 waveform and decreased the percentage of F and G waveforms. The high trehalose and glucose diets increased the percentage of E2 waveform of A. pisum red biotype. The correlation between feeding behavior and sugar contents indicated that the percentage of E1 and E2 waveforms were increased but np, C, F and G waveforms were decreased in low trehalose and glucose contents. The percentage of np, E1 and E2 waveforms were reduced but C, F and G waveforms were elevated in high trehalose and glucose contents. The results suggest that the A. pisum with high trehalose and glucose contents spent less feeding time during non-probing phase and phloem feeding phase, but had an increased feeding time during probing phase, stylet work phase and xylem feeding phase.

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