Desferrioxamine E produced by an indigenous salt tolerant Pseudomonas stutzeri stimulates iron uptake of Triticum aestivum

Chinese Spring (CS) wheat (Triticum aestivum L.) is commonly used in genetic research including cytogenetic analysis, molecular mapping and germplasm development. Aneuploid lines of alien chromosomes in the CS background have been used in studies with diverse objectives. Thousands of genomic and complementary DNA sequences from expressed sequence tag (EST) libraries of biotic- and abiotic-stressed tissues are publicly available from CS. Gene expression analysis of salt-tolerant wheat lines, W4909 and W4910, compared with the CS common wheat background led to the discovery of several expressed sequences that were absent in the CS accession held in our laboratory. A survey of 20 CS accessions from 13 laboratories using the polymerase chain reaction with gene-specific primers for eight salt-responsive genes resulted in amplification success ranging from 15 to 100%. Amplified fragment length polymorphism analysis showed that 99% of the genetic variation was among the accessions while the remaining 1% was within the accessions. A neighbour-joining phylogram showed that four of the five CS accessions from the International Maize and Wheat Improvement Center (CIMMYT) grouped with the salt-tolerant wheat cultivar Yecora Rojo while the remaining 16 CS accessions had limited genetic differences. Thus, variation exists among these highly self-pollinating CS sources, suggesting that appropriate consideration should be taken when using CS accessions to conduct molecular and genetic analyses.

Download Full-text

Compatible salt-tolerant rhizosphere microbe-mediated induction of phenylpropanoid cascade and induced systemic responses against Bipolaris sorokiniana (Sacc.) Shoemaker causing spot blotch disease in wheat ( Triticum aestivum L.)

Applied Soil Ecology ◽

10.1016/j.apsoil.2016.09.014 ◽

2016 ◽

Vol 108 ◽

pp. 300-306 ◽

Cited By ~ 11

Author(s):

Udai B. Singh ◽

Deepti Malviya ◽

Wasiullah ◽

Shailendra Singh ◽

Mohd. Imran ◽

...

Keyword(s):

Triticum Aestivum ◽

Bipolaris Sorokiniana ◽

Triticum Aestivum L ◽

Spot Blotch ◽

Salt Tolerant ◽

Systemic Responses

Download Full-text

Influence of biopreparation ‘Zamin-M’ on cotton plants (gossypium hirsutum) under soil salinization in Uzbekistan

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/939/1/012046 ◽

2021 ◽

Vol 939 (1) ◽

pp. 012046

Author(s):

M K Khojanazarova ◽

S S Murodova ◽

S F Sanakulov ◽

J M Turdaliev

Keyword(s):

Bacillus Subtilis ◽

Pseudomonas Stutzeri ◽

State Committee ◽

Soil Salinization ◽

Agricultural Practice ◽

Experimental Sample ◽

Salt Tolerant ◽

Biological Product ◽

Cotton Plants ◽

The Republic

Abstract In this article, a microbial inoculant acting as a biofertilizer is considered as a biostimulant, consisting of three strains of local salt-tolerant strains of rhizobacteria Pseudomonas stutzeri SKB308, Bacillus subtilis SKB309, Bacillus megaterium SKB310. The authors have developed and introduced into agricultural practice an experimental sample of the biological product ‘Zamin-M’, which increases the resistance of cotton to stress conditions of soil salinity. It is proved that the biological product ‘Zamin-M’ increases soil fertility has a beneficial effect on the enzymatic activity of the soil and the development of a population of beneficial microorganisms. The bioproduct ‘Zamin-M’ is presented as a new stimulant in the practice of growing cotton in saline conditions. An agricultural technique for the use of a local biological product in agriculture has been developed. The ‘Zamin-M’ is included by the State Committee of the Republic of Uzbekistan in the list of agrochemicals and pesticides permitted for use in agriculture of the Republic of Uzbekistan (certificate No. 1A1005).

Download Full-text

A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat

10.1101/2020.01.21.909887 ◽

2020 ◽

Cited By ~ 1

Author(s):

Chana Borjigin ◽

Rhiannon K. Schilling ◽

Jayakumar Bose ◽

Maria Hrmova ◽

Jiaen Qiu ◽

...

Keyword(s):

Triticum Aestivum ◽

Bread Wheat ◽

Salinity Tolerance ◽

Nucleotide Substitution ◽

Wheat Cultivars ◽

Single Nucleotide Substitution ◽

Salt Tolerant ◽

Gene Encoding ◽

Single Nucleotide ◽

Naturally Occurring

AbstractImproving salinity tolerance in the most widely cultivated cereal, bread wheat (Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up to 6 folds greater leaf and sheath sodium (Na+) than two Australian cultivars, Gladius and Scout, under salt stress. Despite high leaf and sheath Na+ concentrations, Mocho de Espiga Branca maintained similar salinity tolerance compared to Gladius and Scout. A naturally occurring single nucleotide substitution was identified in the gene encoding a major Na+ transporter TaHKT1;5-D in Mocho de Espiga Branca, which resulted in a L190P amino acid residue variation. This variant prevents Mocho de Espiga Branca from retrieving Na+ from the root xylem leading to a high shoot Na+ concentration. The identification of the tissue tolerant Mocho de Espiga Branca will accelerate the development of more elite salt tolerant bread wheat cultivars.

Download Full-text

A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes

Plant Cell Reports ◽

10.1007/s00299-010-0868-y ◽

2010 ◽

Vol 29 (8) ◽

pp. 835-844 ◽

Cited By ~ 60

Author(s):

Mahdi Rahaie ◽

Gang-Ping Xue ◽

Mohammad R. Naghavi ◽

Houshang Alizadeh ◽

Peer M. Schenk

Keyword(s):

Triticum Aestivum ◽

Triticum Aestivum L ◽

Salt Tolerant ◽

Myb Gene ◽

Salt And Drought Stresses

Download Full-text

Assessing the Adaptive Mechanisms of Two Bread Wheat (Triticum aestivum L.) Genotypes to Salinity Stress

Agronomy ◽

10.3390/agronomy11101979 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1979

Author(s):

Ulkar Ibrahimova ◽

Zarifa Suleymanova ◽

Marian Brestic ◽

Alamdar Mammadov ◽

Omar M. Ali ◽

...

Keyword(s):

Lipid Peroxidation ◽

Triticum Aestivum ◽

Salt Stress ◽

Salinity Stress ◽

Osmotic Potential ◽

Soluble Sugars ◽

Triticum Aestivum L ◽

Salt Tolerant ◽

Wheat Genotypes ◽

Tolerant Genotype

This work deals with the assessment of physiological and biochemical responses to salt stress, as well as the regulation of the expression of the K+/Na+ transporter gene-TaHKT1;5 of two Triticum aestivum L. genotypes with contrasting tolerance. According to the observations, salinity stress caused lipid peroxidation; accumulation of soluble sugars and proline; decreased osmotic potential, Fv/Fm value, and K+/Na+ ratio; and increased the activity of antioxidant enzymes in both genotypes. In the salt-tolerant genotype, the activity of enzymes, the amounts of soluble sugars and proline were higher, the osmotic potential and the lipid peroxidation were lower than in the sensitive one, and the Fv/Fm value remained unchanged. A comparison of the accumulation of Na+ and K+ ions in the roots and leaves showed that the Na+ content in the leaves is lower. The selective transport of K+ ions from roots to leaves was more efficient in the salt-tolerant genotype Mirbashir-128; consequently, the K+/Na+ ratio in the leaves and roots of this genotype was higher compared with the sensitive Fatima genotype. The semi-quantitative RT-PCR expression experiments on TaHKT1;5 indicated that this gene was not expressed in the leaf of the wheat genotypes. Under salt stress, the expression level of the TaHKT1;5 gene increased in the root tissues of the salt-sensitive genotype, while it decreased in the salt-tolerant wheat genotype. The results obtained suggest that the ion status and salt tolerance of the wheat genotypes are related to the TaHKT1;5 gene activity.

Download Full-text