Boosting accumulation of neutral lipids in Rhodosporidium kratochvilovae HIMPA1 grown on hemp (Cannabis sativa Linn) seed aqueous extract as feedstock for biodiesel production

2014 ◽  
Vol 165 ◽  
pp. 214-222 ◽  
Author(s):  
Alok Patel ◽  
Mohammad Pravez ◽  
Farha Deeba ◽  
Vikas Pruthi ◽  
Rajesh P. Singh ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Cannabis Sativa ◽  
Neutral Lipids ◽  
Biodiesel Production ◽  
Rhodosporidium Kratochvilovae

scholarly journals IN VITRO STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS AND THEIR INTERRELATIONSHIP

2018 ◽  
Vol 11 (4) ◽  
pp. 195 ◽  
Author(s):  
Shubhi Rastogi ◽  
Mohammed Shariq Iqbal ◽  
Deepak Ohri
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Aqueous Extract ◽  
Cannabis Sativa ◽  
Methanolic Extract ◽  
In Vitro Study ◽  
Strong Positive Correlation ◽  
Total Antioxidant ◽  
Anti Inflammatory

 Objective: The objective of the present work is to study the in vitro anti-inflammatory and antioxidant activity of medicinal plants. The extent and correlation between anti-inflammatory and antioxidant activity have been studied.Method: Methanolic and aqueous extracts of five medicinal plants, namely, Ficus racemosa, Aloe vera, Cannabis sativa, Datura stramonium, and Calotropis gigantean have been taken for in vitro anti-inflammatory and total antioxidant activity.Result: The study showed that the inhibition of protein (albumin) denaturation was maximum in aqueous extract of A. vera with 97.55±1.45%. Proteinase inhibitory action of different plant extracts showed significant action and was found to be maximum in aqueous extract of D. stramonium with 87.89±2.58%. Heat-induced hemolysis showed that maximum inhibition was with aqueous extract of F. racemosa with 90.72±3.33%. When hypotonicity-induced hemolysis activity was done it was found maximum in methanolic extract of C. gigantea with 90.58±3.04%. Anti-lipoxygenase activity was found maximum in methanolic extract of F. racemosa with 94.05±4.24%. When total antioxidant activity was done, it was found highest in F. racemosa (4.38±0.546 mM equivalent of ascorbic acid/g tissue).Conclusion: An overall strong positive correlation between anti-inflammatory and antioxidant activity was observed, indicating that antioxidant activity of the plant species studied might be responsible for their anti-inflammatory property. Further work needs to be undertaken to fully elucidate the antioxidants responsible for anti-inflammatory action and to develop better herbal drug formulations.

scholarly journals Rapid Accumulation of Total Lipid inRhizoclonium africanumKutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Gour Gopal Satpati ◽  
Sanjit Kanjilal ◽  
Rachapudi Badari Narayana Prasad ◽  
Ruma Pal
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Structural Changes ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Phosphate Limitation ◽  
Growth Media ◽  
Electron Microscopic ◽  
High Accumulation

Increase of total lipid and the proportion of the favorable fatty acids in marine green filamentous macroalgaRhizoclonium africanum(Chlorophyceae) was studied under nitrate and phosphate limitations. These stresses were given by both eliminating and doubling the required amounts of nitrate and phosphate salts in the growth media. A significant twofold increase in total lipid (193.03 mg/g) was achieved in cells in absence of nitrate in the culture medium, followed by phosphate limitation (142.65 mg/g). The intracellular accumulation of neutral lipids was observed by fluorescence microscopy. The scanning electron microscopic study showed the major structural changes under nutrient starvation. Fourier transform infrared spectroscopy (FTIR) revealed the presence of ester (C-O-C stretching), ketone (C-C stretching), carboxylic acid (O-H bending), phosphine (P-H stretching), aromatic (C-H stretching and bending), and alcohol (O-H stretching and bending) groups in the treated cells indicating the high accumulation of lipid hydrocarbons in the treated cells. Elevated levels of fatty acids favorable for biodiesel production, that is, C16:0, C16:1, C18:1, and C20:1, were identified under nitrate- and phosphate-deficient conditions. This study shows that the manipulation of cultural conditions could affect the biosynthetic pathways leading to increased lipid production while increasing the proportion of fatty acids suitable for biodiesel production.

scholarly journals Producing Energy-Rich Microalgae Biomass for Liquid Biofuels: Influence of Strain Selection and Culture Conditions

Energies ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1246
Author(s):  
Vladimir Heredia ◽  
Olivier Gonçalves ◽  
Luc Marchal ◽  
Jeremy Pruvost
Keyword(s):  
Energy Storage ◽  
Nitrogen Limitation ◽  
Neutral Lipids ◽  
Culture Conditions ◽  
Biodiesel Production ◽  
Biofuel Production ◽  
Freshwater Species ◽  
Nannochloropsis Gaditana ◽  
Parachlorella Kessleri ◽  
Four Levels

Energy-storage metabolites such as neutral lipids and carbohydrates are valuable compounds for liquid biofuel production. The aim of this work is to elucidate the main biological responses of two algae species known for their effective energy-rich compound accumulation in nitrogen limitation and day–night cycles: Nannochloropsis gaditana, a seawater species, and Parachlorella kessleri, a freshwater species. Lipid and carbohydrate production are investigated, as well as cell resistance to mechanical disruption for energy-rich compound release. Nitrogen-depleted N. gaditana showed only a low consumption of energy-storage molecules with a non-significant preference for neutral lipids (TAG) and carbohydrates in day–night cycles. However, it did accumulate significantly fewer carbohydrates than P. kessleri. Following this, the highest levels of productivity for N. gaditana in chemostat cultures at four levels of nitrogen limitation were found to be 3.4 and 2.2 × 10−3 kg/m2·d for carbohydrates and TAG, respectively, at 56%NO3 limitation. The cell disruption rate of N. gaditana decreased along with nitrogen limitation, from 75% (at 200%NO3) to 17% (at 13%NO3). In the context of potentially recoverable energy for biofuels, P. kessleri showed good potential for biodiesel and high potential for bioethanol; by contrast, N. gaditana was found to be more efficient for biodiesel production only.

scholarly journals Biodiesel Production from Oleaginous Fungi

2012 ◽  
Vol 6 (1) ◽  
pp. 43-49 ◽  
Author(s):  
K Ravikumar ◽  
J Dakshayini ◽  
ST Girisha
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Palmitic Acid ◽  
Neutral Lipids ◽  
Fatty Acyl ◽  
Ethyl Esters ◽  
Biodiesel Production ◽  
Sudan Black B ◽  
Oleaginous Fungi ◽  
Micro Organisms

Biodiesel involves the mixture of fatty acyl methyl/ethyl esters, produced from transesterification neutral lipids and if the origin of the source is from oleaginous micro organisms, then it is termed as micro diesel. In the present work, aiming to exploit fungi for biodiesel production, 12 fungal isolates were screened for lipid content by Sudan Black B staining method. Among 12 isolates, lipid rich five species viz, Mortierella alpina , M.ramanianna, M.vinancea, M.hyalina and M.verticella have been taken for fatty acids analysis by spectrophotometry, which revealed that the amount of free fatty acids were ranged from highest in M.alpina 35 ?moles of Oleic acid , 25 ?moles of Palmitic acid and 14 ?moles of Myristic acids to lower as much as 21 ?moles of Oleic acid , 18 ?moles of Palmitic acid and 16 ?moles of Myristic acids respectively in M.ramanianna. DOI: http://dx.doi.org/10.3126/ijls.v6i1.5721

Cannabis sativa L. (Marijuana) V: Pharmacological Evaluation of Marijuana Aqueous Extract and Volatile Oil

1974 ◽  
Vol 63 (6) ◽  
pp. 962-964 ◽  
Author(s):  
Alvin B. Segelman ◽  
R. Duane Sofia ◽  
Florence P. Segelman ◽  
John J. Harakal ◽  
Linda C. Knobloch
Keyword(s):  
Aqueous Extract ◽  
Cannabis Sativa ◽  
Volatile Oil ◽  
Pharmacological Evaluation

scholarly journals Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Redouane Benhima ◽  
Hicham El Arroussi ◽  
Issam M. Kadmiri ◽  
Najib El Mernissi ◽  
Imane Wahby ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Sodium Tungstate ◽  
Neutral Lipids ◽  
Dunaliella Tertiolecta ◽  
Biodiesel Production ◽  
Nitrogen Stress ◽  
Slight Variation ◽  
Two Stage ◽  
One Stage ◽  
Two Stage Culture

Nitrogen stress increases lipids content in microalgae, the main feedstock for algal biodiesel. Sodium tungstate was used in this study to implement nitrogen stress by inhibiting nitrate reductase (NR) in Dunaliella tertiolecta. The reduction of NR activity was accompanied by reduction of chlorophyll and accumulation of lipids. One-stage and two-stage culture strategies were compared. One-stage culture raised total lipids from 18% (control) to 39% (w: w); however, two-stage culture raised lipids to 50% in which neutral lipids were enhanced 2.14 times. To assess the quality of biodiesel produced, fatty acid methyl esters (FAME) composition was studied. It showed a slight variation of unsaturation. In addition, some physical proprieties of biodiesel were estimated and showed that higher heating values were improved by tungstate treatment. In this study, we tried to shed light on some biological impact of NR inhibition in microalgae cells using sodium tungstate which could be exploited in the improvement of biodiesel production.

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