scholarly journals Characterization of a novel strain of Tribonema minus demonstrating high biomass productivity in outdoor raceway ponds

2021 ◽  
pp. 125007
Author(s):  
Aubrey K. Davis ◽  
Ryan S. Anderson ◽  
Ruth Spierling ◽  
Sara Leader ◽  
Carly Lesne ◽  
...  
Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 460 ◽  
Author(s):  
Juan Eduardo Sosa-Hernández ◽  
Laura Isabel Rodas-Zuluaga ◽  
Carlos Castillo-Zacarías ◽  
Magdalena Rostro-Alanís ◽  
Reynaldo de la Cruz ◽  
...  

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.


Energies ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 341 ◽  
Author(s):  
Annakkili Baskara Boopathy ◽  
Thanasekaran Jayakumar ◽  
Senthil Chinnasamy ◽  
Muthu Ganesan Rajaram ◽  
Natarajan Mohan ◽  
...  

Four different strains of marine algae viz. Tetraselmis tetrathele, Tetraselmis striata, Tetraselmis chuii, and Tetraselmis gracilis were isolated from the saltpans in Kovelong, Chennai, Tamil Nadu, India. The systematic position of Tetraselmis striata BBRR1 was confirmed through molecular identification. Under laboratory conditions, T. striata Butcher BBRR1 grown in f/2-medium recorded highest biomass concentration of 0.58 ± 0.021 g L−1, volumetric productivity of 0.025 ± 0.004 g L−1 d−1, 19 ± 2.3% proteins, 17 ± 1.5% carbohydrates, and 15 ± 2.4% lipids. Volumetric biomass productivity of 0.063 ± 0.08 g L−1 d−1, specific growth rate of 0.45 day−1 and lipid content of 19.42 ± 0.98% were recorded for the alga T. striata Butcher BBRR1 cultivated in 10-m2 open raceway ponds using Modified CFTRI ABRR1 medium. The fatty acid profile of T. striata Butcher BBRR1 showed the presence of 33.14% palmitic acid, 22.64% 11-octadecenoic acid, and 21.94% heptadecanoic acid. This study confirms the feasibility of cultivating the marine alga T. striata in open raceway ponds to produce biomass, which can be used for the production of biofuels.


2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Thi Cam Van Do ◽  
Dang Thuan Tran ◽  
Truong Giang Le ◽  
Quang Trung Nguyen

Microalgae have been reported to produce endogenous phytohormones including auxins, gibberellins, cytokinins, brassinosteroids, and abscisic acid. Methanol residual released from microalgal lipid extraction usually contains a variety of bioactive compounds including the phytohormones; however, they are poorly characterized and used for other applications. This study aimed at investigating auxin, gibberellin, and cytokinin production of C. sorokiniana TH01 under phototrophic and mixtrophic cultivations. Moreover, endogenous auxins, gibberellins, and cytokinins in methanol residual obtained from the algal lipid extraction were characterized using HPLC-ESI-MS/MS toward application for crop and biorefinery research. Data showed that endogenous indole-3-acetic acid (IAA), 3-indolepropionic acid (IPA), gibberellin A4 (GA4), and gibberellin A7 (GA7) were detected in C. sorokiniana TH01 biomass. Under the phototrophic mode, total auxin and GA levels were reduced to 0.98 and 9.65 μg/g DW under salt stress (20 g NaCl/L) from 3.59 to 24.71 μg/g DW, respectively, measured for the control. Similarly, total auxins and GAs were also decreased to 0.56 and 2.86 μg/g DW, respectively, under mixtrophic growth with 6 g glucose/L. Total auxins and GAs determined in the water algal extract were 1062.7 and 2000.1 μg/L, respectively. Treatment with higher 40% (v/v) of the algal extract triggered earlier seed germination of rice and tomato plants in 2 and 1 days, respectively. Our new findings in capability of C. sorokiniana TH01 in endogenous phytohormone production contain fundamental merits for further optimization of the algal production (i.e., cultivation modes, conditions, lipids, biomass productivity, and hormone levels) to be used for biorefinery.


Lethaia ◽  
2018 ◽  
Vol 52 (2) ◽  
pp. 165-187 ◽  
Author(s):  
Morgane Brosse ◽  
Hugo Bucher ◽  
Aymon Baud ◽  
Åsa M. Frisk ◽  
Nicolas Goudemand ◽  
...  

2021 ◽  
Author(s):  
Fang Xiao ◽  
Fangqing Weng ◽  
Duyu Guo ◽  
Ernest Koranteng ◽  
Hongbin Zhou ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1347
Author(s):  
Elena Sánchez ◽  
Gladys Lino ◽  
Xavier Serrat ◽  
Salvador Nogués

The present study assessed the behavior of four clones of Arundo donax L. (giant reed) as a perennial rhizomatous grass of increasing interest due to its high biomass production and great adaptability to stress conditions. In this study, a molecular, physiological, and biomass characterization was performed in greenhouse conditions on four Mediterranean clones. The majority of physiological and biomass parameters were not significantly different between clones. However, it was possible to observe large differences in the chromosome count for the four clones. In this way, we detected different numbers of chromosomes for each clone (98 to 122), but surprisingly, no correlation was observed between their chromosome numbers and their physiological and biomass responses.


2020 ◽  
Author(s):  
Jose J De Vega ◽  
Ned Peel ◽  
Sarah J Purdy ◽  
Sarah Hawkins ◽  
Iain Donnison ◽  
...  

ABSTRACTMiscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity and low chemical input requirements. Interspecific Miscanthus hybrids with high biomass yield were shown to have low concentrations of starch and sucrose but high concentrations of fructose. We performed a transcriptional RNA-seq analysis between selected Miscanthus hybrids with contrasting values for these phenotypes to clarify how these phenotypes are genetically controlled. We observed that genes directly involved in the synthesis and degradation of starch and sucrose were down-regulated in high yielding Miscanthus hybrids. At the same time, glycolysis and export of triose phosphates were up-regulated in high yielding Miscanthus hybrids. Our results evidence a direct relationship between high expression of essential enzymatic genes in the starch and sucrose pathways, high starch concentrations, and lower biomass production. The strong interconnectivity between genotype, chemotype and agronomic traits opens the door to use the expression of well-characterised genes in the starch and sucrose pathway for the early selection of high biomass yielding genotypes from large Miscanthus populations.


Author(s):  
Riny Modaso ◽  
Edi Suryanto ◽  
Trina Tallei ◽  
Inneke F.M Rumengan

Chitin and chitosan from rotifer have not been previously explored due to the problem of high biomass required for extraction. This study aimed to obtain the chitin yield from rotifer biomass produced in mass culture, and to characterize the basic properties of chitin and chitosan, especially, nitrogen content and dye binding capacity. Methods of extraction and deacetylation of chitin were adopted from Chandumpai et al. (2004) with modification. The nitrogen content was analyzed using the semi-micro Kjeldahl method (AOAC, 1984), and dye binding capacity using the method developed by Cho et al. (1998). Results show that the yield of chitin obtained from the rotifer sample was relatively small (4.64%), and the yield of chitosan was even smaller, only 2,62%. The proportion of chitosan over chitin was 52,4%. The nitrogen content of chitin and chitosan of rotifer were 4.23 to 4.36% and 7.12-7.23%, respectively. The capacity of chitin to maintain the bonded dye was relatively stronger than that of chitosan, but the chitosan had higher capacity to absorb the dye. The characterization of other important properties of chitin and chitosan to be developed as biopolymer for industry is further aspects to be assessed© Eksplorasi kitin dan kitosan dari rotifer belum pernah dilakukan sebelumnya karena dihadapkan pada permasalahan kebutuhkan biomassa yang banyak. Penelitian ini bertujuan untuk memperoleh rendemen kitin dari biomassa rotifer hasil kultur massal, dan menjajaki karakterisasi awal sifat fisika kimia kitin yang mendasar yaitu daya ikat zat warna dan kadar nitrogen. Ekstraksi kitin dan kitosan dilakukan berdasarkan metode Chandupai et al. (2004), kadar nitrogen menggunakan metode semi-micro Kjeldahl (AOAC, 1984), dan daya ikat zar warna ditentukan menurut metode Cho et al. (1998). Dari penelitian ini, rendemen kitin yang diperoleh dari hasil ekstraksi relatif kecil berkisar 4,64 %, dan rendemen kitosan bahkan lebih kecil lagi hanya 2,62%. Proporsi kitosan dari kitin yaitu 52.4 %. Kadar nitrogen pada kitin rotifer berkisar 4,23-4,36 % dan kitosan berkisar dari 7,12-7,23%. Kitin memiliki sifat yang lebih baik dalam ketahanan untuk mengikat zat warna sebaliknya kitosan memperlihatkan sifatnya yang dapat mengikat zat warna dalam tingkat adsorpsi yang tinggi dibanding kitin. Karakterisasi  sifat fisika kimia kitin dan kitosan yang penting lainnya untuk pengembangannya sebagai biopolimer dalam skala industri, merupakan aspek-aspek yang perlu dikaji lebih lanjut©


Energies ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2660 ◽  
Author(s):  
Muhammad Maqsood Alam ◽  
Abdul Samad Mumtaz ◽  
Megan Russell ◽  
Melanie Grogger ◽  
Don Veverka ◽  
...  

Production of microalgae as feedstock for biofuels must deal with a number of challenges including constraints imposed by local conditions. One solution is to use indigenous strains adapted to local climatic conditions. The present report describes the isolation, identification, and characterization of 32 microalgal strains from different ecological habitats: desert freshwater channels, northern region, and saline regions of Pakistan. The effects of temperature on algal growth rates, biomass productivity, and lipid content were determined through growth at 12, 20, and 35 °C for 15 days under 2% CO2 Responses to temperature varied among species with 20 °C being the optimum temperature in general, although, exceptionally, the best overall growth rate was found for strain S29 (0.311 d−1) at 12 °C. In some cases high biomass productivity was observed at 35 °C, and, depending upon the strain, the maximum lipid content was obtained at different temperatures, including 12 °C. Fatty acid methyl ester (FAME) analysis showed that the major fatty acids present were palmitic, stearic, oleic, linoleic, and linolenic. Oleic acid (C18:1) was the predominant fatty acid, with the specific FAME profile varying with strain. Thus, there is a rich diversity of microalgal strains native to Pakistan, some of which, characterized here, could be suitable for biodiesel production or other biotechnological applications.


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