scholarly journals Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 460 ◽  
Author(s):  
Juan Eduardo Sosa-Hernández ◽  
Laura Isabel Rodas-Zuluaga ◽  
Carlos Castillo-Zacarías ◽  
Magdalena Rostro-Alanís ◽  
Reynaldo de la Cruz ◽  
...  
Keyword(s):  
Light Intensity ◽  
Biomass Yield ◽  
Nitrogen Concentration ◽  
Biomass Productivity ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Biomass ◽  
Porphyridium Purpureum ◽  
Nitrogen Concentrations ◽  
Low Nitrogen

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

scholarly journals Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 124 ◽  
Author(s):  
Tao Li ◽  
Jin Xu ◽  
Houbo Wu ◽  
Peiliang Jiang ◽  
Zishuo Chen ◽  
...  
Keyword(s):  
Large Scale ◽  
Culture Medium ◽  
Nitrogen Concentration ◽  
Nitrogen Supply ◽  
Arachidonic Acid Content ◽  
Porphyridium Purpureum ◽  
Strong Light ◽  
Commercial Cultivation ◽  
Nitrogen Concentrations ◽  
Low Nitrogen

Microalgae of the genus Porphyridium show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce Porphyridium biomass rich in B-PE, LC-PUFAs and EPS. Porphyridium purpureum SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light.

scholarly journals Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Indrayani Indrayani ◽  
Haslianti Haslianti ◽  
Asmariani Asmariani ◽  
Wellem Muskita ◽  
Abdul Muis Balubi
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
High Growth ◽  
Biomass Productivity ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Growth Rate ◽  
Lipid Productivity ◽  
Marine Diatom ◽  
Light Intensities

Abstract. Indrayani I, Haslianti H, Asmariani A, Muskita WH, Balubi M. 2020. Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas 21: 1498-1503. Light is one of the important factors affecting growth and biochemical composition of microalgae. The aim of this study was to determine growth, biomass and lipid productivity of a newly isolated marine diatom, Skeletonema sp.UHO29 under different light intensities. The Skeletonema sp. was cultured in 300 mL conical flasks containing 150 mL of f/2 medium under three different light intensities (28, 60 and 110 μmol photons m-2 s-1 ) with three replications. The cultures were incubated under ambient room temperatures, and a cycle of 12 hours light and 12 hours dark, in batch modes for two weeks. The results showed that the highest specific growth rate (0.56 d-1) was achieved at the highest light intensity (110 μmol photons m-2 s-1) and the lowest (0.11 d-1) was obtained at the lowest light intensity (28 μmol photons m-2 s-1). The highest biomass yield (0.62 g L-1) and biomass productivity (0.34 g L-1 d-1) was achieved at the highest light intensity. However, the highest lipid yield (0.175 g L-1), lipid content (28.78% ash-free dry weight) and lipid productivity (0.067 g L-1 d-1) were achieved at medium light intensity. The alga prefers high light intensity for higher growth rate and biomass productivity but it produces more lipids at medium light intensity. This study indicated that the Skeletonema sp.UHO29 is a potential species for outdoor mass cultivation and is a promising microalgal strain for use as a biodiesel feedstock due to its high growth rate, high biomass and lipid productivity.

Analyzing and optimizing the carbon utilization and lipid yield at different light intensities for Scenedesmus arcuatus

2021 ◽  
Vol 25 (11) ◽  
pp. 1-10
Author(s):  
K. Vasumathi ◽  
Raja Vadivu G. Nadana ◽  
E.M. Nithiya ◽  
K. Sundar ◽  
M. Premalatha
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Biomass Yield ◽  
Osmotic Shock ◽  
High Light Intensity ◽  
Carbon Utilization ◽  
Lipid Yield ◽  
The Face ◽  
Photosynthetic Microorganism ◽  
Pre Treatment

Microalgae, the photosynthetic microorganism growing abundantly in marine and aquatic ecosystems, are potential source for biological sequestration of CO2. The carbon uptake differs in the presence of other nutrients, light intensity etc. The biomass yield of Scenedesmus arcuatus var capitatus was studied based on the Face Centred Central Composite design (FCCD) of Response Surface Methodology (RSM) for nitrate, phosphate and carbonate under different conditions (laboratory, room and sunlight conditions). Various pre-treatments (osmotic shock, autoclaving, microwave and ultrasonication) were employed to find the best method for maximum lipid yield. The biomass yield reached a maximum of 1 g/L under sunlight conditions of nitrate concentration 500 ppm and carbonate 2000 ppm. The laboratory conditions resulted in a biomass yield of 0.59 g/L at 500 ppm nitrate, 1000 ppm carbonate and 250 ppm phosphate. Under room conditions, the yield was very low (0.11 g/L). Osmotic shock resulted in higher lipid yield than the other pre-treatment methods. The ability of Scenedesmus arcuatus to uptake high carbon under sunlight conditions and to adapt to high light intensity and fluctuations in light intensity concludes that this species is suitable for large-scale open pond cultivation for CO2 sequestration and production of metabolites.

Effects of nitrogen and lime on growth of Leucaena leucocephala cv. Cunningham on a red-yellow podzolic soil in south-eastern Queensland

1986 ◽  
Vol 26 (1) ◽  
pp. 23 ◽  
Author(s):  
S Sivasupiramaniam ◽  
R Akkasaeng ◽  
HM Shelton
Keyword(s):  
Podzolic Soil ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
Maximum Growth ◽  
Field Application ◽  
South Eastern ◽  
Shoot Dry Weight ◽  
Application Rates ◽  
Lime Application ◽  
Low Nitrogen

Field and glasshouse experiments were conducted on an acidic red-yellow podzolic soil of low nitrogen status from south-eastern Queensland to examine the use of nitrogen and lime in promoting leucaena growth. In the field, application of 25 kg nitrogen ha-l at planting increased shoot dry weight by 77, 88, 52 and 51% at weeks 5, 15, 32 and 52 respectively. Equivalent responses to lime (2.5 t ha-1) were 8, 3 1, 64 and 74% respectively. In the glasshouse, shoot, root and nodule dry weights were increased by nitrogen application rates of 50, 100 and 200 kg ha-1. Number, size and dry weight of nodules were suppressed at the highest rate (400 kg ha-1). Nitrogen concentration in youngest fully expanded leaves was increased to over 5% by nitrogen, and a critical nitrogen content of 4.5% (90% of maximum growth) is suggested. Inexplicably, lime application reduced shoot, root and nodule weights at high nitrogen applications. The apparent partial effectiveness of commercial leucaena Rhizobium and the implications of starter nitrogen and lime for leucaena establishment are discussed.

Response of abscisic acid mutants of Arabidopsis to salinity

2002 ◽  
Vol 29 (5) ◽  
pp. 561 ◽  
Author(s):  
Grant R. Cramer
Keyword(s):  
Abscisic Acid ◽  
Light Intensity ◽  
Salinity Stress ◽  
Root Elongation ◽  
High Salinity ◽  
Dry Weight ◽  
Protective Role ◽  
High Light Intensity ◽  
High Light ◽  
Total Dry Weight

Increases in abscisic acid (ABA) concentrations in plant tissues correlate with growth inhibition in salt-stressed plants. Therefore, it was hypothesized that Arabidopsis ABA mutants different in, or insensitive to, ABA would respond differently than wild type (wt) to salinity stress. Seeds (wt, abi1-1, abi2-1, abi3-1, and aba1-3) were germinated and grown hydroponically in three separate experiments with different environmental conditions: relative humidity at 80 or 100%, day/night temperatures at 21/18 or 23/20˚C, and light intensity at 125, 200 or 350 μmol photons m–2 s-1. Plants were exposed to salinity (either 0, 40 and 80 mM NaCl or 1, 5, and 9 dS m–1 with a Na/Ca ratio of 10 depending on the experiment) for one to several weeks before harvesting. The effect of salinity on root elongation rates of young seedlings was measured as well. Two-way ANOVA of root elongation rates of young seedlings and the growth of 3-week old plants in hydroponic solutions indicated that salinity inhibited growth, increased ABA and Na concentrations, and reduced K concentrations in all genotypes tested. However, there were no significant interactions with salinity and genotype for root elongation rates, total dry weight, shoot ABA and K concentrations. Shoot Na concentrations were significantly higher in wt plants relative to other genotypes subjected to high salinity stress. aba1-3 had significantly lower ABA concentrations than other genotypes, but the interaction of aba1-3 with salinity was the same as other genotypes. The lack of difference in interaction between genotype and salinity indicates that all genotypes responded in the same manner and amount to salinity for the particular parameter measured. Therefore, it appears that there are no significant differences in growth in response to salinity between the ABA mutants (ABA-deficient and ABA-insensitive) and wt. However, in contrast to the other genotypes, some of the ABA-deficient plants, aba1-3, died when exposed to high salinity and high light intensity. ABA appears to provide a protective role in conditions of high salinity and high light intensity.

scholarly journals Substrate Acidification by Geranium: Light Effects and Phosphorus Uptake

2008 ◽  
Vol 133 (4) ◽  
pp. 515-520 ◽  
Author(s):  
Matthew D. Taylor ◽  
Paul V. Nelson ◽  
Jonathan M. Frantz
Keyword(s):  
Light Intensity ◽  
Phosphorus Uptake ◽  
Dry Weight ◽  
P Uptake ◽  
High Light Intensity ◽  
High Light ◽  
P Deficiency ◽  
Light Levels ◽  
Direct Light ◽  
Substrate Ph

Sudden pH decline (SPD) describes the situation where crops growing at an appropriate pH rapidly (within 1–2 weeks) cause the substrate pH to shift downward one to two units. ‘Designer Dark Red’ geraniums (Pelargonium ×hortorum Bailey) were grown in three experiments to assess possible effects of light on SPD and phosphorous (P) uptake. The first experiment tested the effect of four light intensities (105, 210, 575, and 1020 ± 25 μmol·m−2·s−1) on substrate acidification. At 63 days, substrate pH declined from 6.0 to 4.8 as light intensity increased. Tissue P of plants grown at the highest two light levels was extremely low (0.10%–0.14% of dry weight). P stress has been reported to cause acidification. Because plants in the two lowest light treatments had adequate P, it was not possible to determine if the drop in substrate pH was a direct light effect or a combination of light and P. The second experiment used a factorial combination of the three highest light levels from Expt. 1 and five preplant P rates (0, 0.065, 0.13, 0.26, or 0.52 g·L−1 substrate) to assess this question. When tissue P concentrations were deficient, pH decreased by 0.6 to 1.0 pH units within 2 weeks and deficiency occurred more often with high light intensity. These data indicated that P deficiency caused substrate acidification and indicated the possibility that P uptake was suppressed by high light intensity. The third experiment was conducted in hydroponics to determine the direct effect of high light intensity on P uptake. In this experiment, cumulative P uptake per gram root and the rate of P uptake per gram root per day both decreased 20% when light intensity increased from 500 to 1100 μmol·m−2·s−1. It is clear from this study that P deficiency causes geraniums to acidify the substrate and that high light suppresses P uptake.

scholarly journals Influence of Medium Frequency Light/Dark Cycles on the Cultivation of Auxenochlorella pyrenoidosa

2020 ◽  
Vol 10 (15) ◽  
pp. 5093
Author(s):  
Zhijie Chen ◽  
Bosheng Su
Keyword(s):  
Light Intensity ◽  
Biomass Production ◽  
Cycle Time ◽  
Biomass Yield ◽  
Continuous Light ◽  
Biomass Productivity ◽  
Chlorella Pyrenoidosa ◽  
Dark Cycle ◽  
Medium Frequency ◽  
Intermittent Light

Light (wavelength, intensity, and light/dark cycle) have been considered as one of the most important parameters for microalgae cultivation. In this paper, the effect of medium frequency intermittent light on Auxenochlorella pyrenoidosa (formerly Chlorella pyrenoidosa) cultivation was investigated. Three parameters of intermittent light, light intensity, light/dark ratio, and light/dark cycle were employed and the influence of these parameters on the productivity of Auxenochlorella pyrenoidosa was studied. The biomass yield and growth rates were mainly affected by the light fraction and cycle time. Light with 220 μE m−2 s−1 light intensity was determined as the optimal light intensity for biomass production. At the light intensity of 420 μE m−2 s−1, the results indicated that the intermittent light improved the biomass production with larger light/dark ratio compared with the continuous light. At a lower mean light intensity over time, the intermittent light should be more suitable for biomass growth and the decrease in the light/dark ratio (L/D) will lead to a higher biomass productivity. The light/dark cycle time has little influence on the biomass yield.

scholarly journals Perbedaan Kebutuhan Nitrogen untuk Produksi Kedelai di Tanah Mineral dan Mineral Bergambut Dengan Budidaya Jenuh Air

2016 ◽  
Vol 35 (3) ◽  
pp. 217 ◽  
Author(s):  
Bachtiar ◽  
Munif Ghulamahdi ◽  
Maya Melati ◽  
Dwi Guntoro ◽  
Atang Sutandi
Keyword(s):  
Block Design ◽  
Nitrogen Concentration ◽  
Mineral Soil ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Land Type ◽  
Randomized Block Design ◽  
Nitrogen Concentrations ◽  
Glycine Max L ◽  
Soybean Plants

Development of soybean (Glycine max (L) Merr.) in tidal land are faced with problems physical, chemical and biological soil properties, such as high organic matter, high soil acidity, toxicity of Fe and Al, and deficiency of nutrients N, P , K, Ca and Mg. N content is high (> 0.51%) but with low availability.  The research objective is to determine the dose and timing of N, P and K application in accordance with the needs of soybean plants to have optimally growth and production in mineral and peaty mineral soil in tidal land. The research was conducted in mineral and peaty mineral soil of tidal land type C and B, District of Tanjung Lago, Banyuasin Regency, Province of South Sumatra from April to August 2014. The model is linear using split plots in a randomized block design. Varieties of Willis and Tanggamus were used for nitrogen application experiment.  The time of fertilizer application is at 2, 3 and 4 weeks after planting (WAP), 2, 3, 4 and 5 WAP, and 2, 3, 4, 5 and 6 WAP. The concentration of nitrogen is 7,5; 10; 12,5 and 15 g/l water with spraying volume of 400 l/ha.  In the mineral soil, dry weight of nodules and Willis biomass increased with increasing frequency time of fertilization, otherwise Tanggamus more fluctuating and declined at higher frequency of fertilization. Wilis variety generating the highest production of 3,5 ton/ha.  In the peaty mineral soil, dry weight of nodules and biomass were not significant.  Willis productivity tends to decrease with increasing nitrogen concentrations.  Tanggamus productivity tends to increase with increasing nitrogen concentration at all level of time fertilization.  Tanggamus tend to generate higher productivity of 3,2 ton/ha.

scholarly journals Production of Lutein-enriched Biomass by Growing Coccomyxa sp. (strain onubensis; Chlorophyta) under Spring Outdoor Conditions of Southwest Spain

2016 ◽  
Vol 6 (1) ◽  
pp. 828-834 ◽  
Author(s):  
Isabel Vaquero ◽  
M. Carmen Ruiz-Domínguez ◽  
Benito Mogedas ◽  
Carlos Vílchez ◽  
José M Vega
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Acidic Environment ◽  
Intensity Maximum ◽  
Tubular Photobioreactor ◽  
Outdoor Culture ◽  
Environmental Light ◽  
Intracellular Content

Coccomyxa sp. (strain onubensis) is an acidic environment microalga, which was grown under spring outdoor conditions of Huelva province (southwest, Spain). A 6 L pilot tubular photobioreactor was used to determine the influence of the environmental light and temperature conditions on the microalga growth and its intracellular content of carotenoids. Cloudy days supplied the adequate light intensity (maximum 1070 μmol photons m-2 s-1) and temperature (maximum 32ºC) regimes for a biomass productivity of 0.28 g L-1 d-1 with an intracellular content of lutein (4.8 mg g-1 dry weight) and eventually β-carotene (0.8 mg g-1) obtained at the end of the day evening. This work supplies guidelines for a large-scale outdoor culture of Coccomyxa. sp. to supply lutein-enriched biomass, with the advantage of the growth at pH 2.5, which preserves cultures from contamination.  

scholarly journals Cytogenotoxic effects of nitrogen on hydroponic lettuce

2020 ◽  
Vol 36 ◽  
Author(s):  
Alcione da Silva Arruda ◽  
Wesley Costa Silva ◽  
Roberta Camargos de Oliveira ◽  
Ernane Miranda Lemes ◽  
Gabriela da Silva Guimarães ◽  
...  
Keyword(s):  
Nitrogen Concentration ◽  
Calcium Nitrate ◽  
Dry Weight ◽  
Positive Control ◽  
Leaf Nitrogen ◽  
Nitrogen Accumulation ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Nutrient Film Technique ◽  
Nitrogen Concentrations

Nitrogen accumulation in hydroponically-grown lettuce may pose a health risk to consumers. Thus, the objective of this study was to analyze different concentrations of nitrogen applications in hydroponic lettuce cultivation and their effect on toxicity, cytotoxicity and genotoxicity. A nutrient film technique (NFT) hydroponic system was used to grow the lettuce variety “Vanda.” The treatments consisted of different concentrations of nitrogen (in the form of calcium nitrate) in Furlani solution (75, 100, 125 and 150%), a negative and a positive control. The following commercial characteristics were measured: plant fresh weight (PFW), root fresh weight (RFW), shoot fresh weight (SFW), shoot diameter (SD), root dry weight (RDW), shoot dry weight (SDW) and leaf nitrogen (LN). Cytogenotoxicity was indicated by toxicity, cytotoxicity and genotoxicity, which were in turn determined by root length, the mitotic index, chromosomal aberrations and the presence of micronuclei.  The nitrogen concentrations used in this experiment did not cause phenotypic toxicity or cytotoxicity in lettuce roots. The most severe genotoxicity was observed at the 125% nitrogen concentration, which nevertheless did not affect commercial characteristics. Although nitrogen fertilization provides great benefits to agriculture, such as greater yields, indiscriminate use should be avoided since concentrations above recommended rates may induce genotoxicity.

Sign in / Sign up

Export Citation Format

Share Document