Comparative study of genotoxic, antigenotoxic and cytotoxic activities of monoterpenes camphor, eucalyptol and thujone in bacteria and mammalian cells

2015 ◽  
Vol 242 ◽  
pp. 263-271 ◽  
Author(s):  
Biljana Nikolić ◽  
Bojana Vasilijević ◽  
Dragana Mitić-Ćulafić ◽  
Branka Vuković-Gačić ◽  
Jelena Knežević-Vukćević
Keyword(s):  
Comparative Study ◽  
Mammalian Cells ◽  
Cytotoxic Activities

scholarly journals Distinct Chemistries Define the Diverse Biological Effects of Plasma Activated Water Generated with Spark and Glow Plasma Discharges

2021 ◽  
Vol 11 (3) ◽  
pp. 1178
Author(s):  
Evanthia Tsoukou ◽  
Maxime Delit ◽  
Louise Treint ◽  
Paula Bourke ◽  
Daniela Boehm
Keyword(s):  
Mammalian Cells ◽  
Biomedical Application ◽  
Biological Effects ◽  
Mode Of Delivery ◽  
Chemical Species ◽  
Resistant Bacteria ◽  
Cytotoxic Activities ◽  
Promising Alternative ◽  
Plasma Activated Water

The spread of multidrug-resistant bacteria poses a significant threat to human health. Plasma activated liquids (PAL) could be a promising alternative for microbial decontamination, where different PAL can possess diverse antimicrobial efficacies and cytotoxic profiles, depending on the range and concentration of their reactive chemical species. In this research, the biological activity of plasma activated water (PAW) on different biological targets including both microbiological and mammalian cells was investigated in vitro. The aim was to further an understanding of the specific role of distinct plasma reactive species, which is required to tailor plasma activated liquids for use in applications where high antimicrobial activity is required without adversely affecting the biology of eukaryotic cells. PAW was generated by glow and spark discharges, which provide selective generation of hydrogen peroxide, nitrite and nitrate in the liquid. The PAW made by either spark or glow discharges showed similar antimicrobial efficacy and stability of activity, despite the very different reactive oxygen species (ROS) and reactive nitrogen species profiles (RNS). However, different trends were observed for cytotoxic activities and effects on enzyme function, which were translated through the selective chemical species generation. These findings indicate very distinct mechanisms of action which may be exploited when tailoring plasma activated liquids to various applications. A remarkable stability to heat and pressure was noted for PAW generated with this set up, which broadens the application potential. These features also suggest that post plasma modifications and post generation stability can be harnessed as a further means of modulating the chemistry, activity and mode of delivery of plasma functionalised liquids. Overall, these results further understanding on how PAL generation may be tuned to provide candidate disinfectant agents for biomedical application or for bio-decontamination in diverse areas.

scholarly journals Chemical Composition and Antibacterial and Cytotoxic Activities ofAllium hirtifoliumBoiss

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Salmiah Ismail ◽  
Farid Azizi Jalilian ◽  
Amir Hossein Talebpour ◽  
Mohsen Zargar ◽  
Kamyar Shameli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Chemical Composition ◽  
Mammalian Cells ◽  
Pathogenic Bacteria ◽  
Cytotoxic Activities ◽  
Hexadecenoic Acid ◽  
Minimum Concentration ◽  
Gram Positive Bacteria ◽  
Microdilution Broth

Allium hirtifoliumBoiss. known as Persian shallot, is a spice used as a traditional medicine in Iran and, Mediterranean region. In this study, the chemical composition of the hydromethanolic extract of this plant was analyzed using GC/MS. The result showed that 9-hexadecenoic acid, 11,14-eicosadienoic acid, and n-hexadecanoic acid are the main constituents. The antibacterial activity of the shallot extract was also examined by disk diffusion and microdilution broth assays. It was demonstrated that Persian shallot hydromethanolic extract was effective against 10 different species of pathogenic bacteria including methicillin resistantStaphylococcus aureus(MRSA), methicillin sensitiveStaphylococcus aureus(MSSA),Staphylococcus aureus,Staphylococcus epidermidis,Streptococcus pneumoniae,Escherichia coli, Escherichia coliO157:H7,Salmonella typhimurium, Proteus mirabilis, andKlebsiella pneumoniae. Specifically, the minimum concentration of the extract which inhibited bacterial growth (MIC values) was 1.88 mg/mL for most of the gram-positive bacteria. This concentration was not much different from the concentration that was safe for mammalian cells (1.50 mg/mL) suggesting that the hydromethanolic extract of Persian shallot may be a safe and strong antibacterial agent.

scholarly journals Clavanin A Improves Outcome of Complications from Different Bacterial Infections

2014 ◽  
Vol 59 (3) ◽  
pp. 1620-1626 ◽  
Author(s):  
Osmar N. Silva ◽  
Isabel C. M. Fensterseifer ◽  
Elaine A. Rodrigues ◽  
Hortência H. S. Holanda ◽  
Natasha R. F. Novaes ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Toxicity Tests ◽  
Cytotoxic Activities ◽  
Content Type ◽  
Wound Model ◽  
Acute Toxicity Tests

ABSTRACTThe rapid increase in the incidence of multidrug-resistant infections today has led to enormous interest in antimicrobial peptides (AMPs) as suitable compounds for developing unusual antibiotics. In this study, clavanin A, an antimicrobial peptide previously isolated from the marine tunicateStyela clava, was selected as a purposeful molecule that could be used in controlling infection and further synthesized. Clavanin A wasin vitroevaluated againstStaphylococcus aureusandEscherichia colias well as toward L929 mouse fibroblasts and skin primary cells (SPCs). Moreover, this peptide was challenged here in anin vivowound and sepsis model, and the immune response was also analyzed. Despite displaying clearin vitroantimicrobial activity toward Gram-positive and -negative bacteria, clavanin A showed no cytotoxic activities against mammalian cells, and in acute toxicity tests, no adverse reaction was observed at any of the concentrations. Moreover, clavanin A significantly reduced theS. aureusCFU in an experimental wound model. This peptide also reduced the mortality of mice infected withE. coliandS. aureusby 80% compared with that of control animals (treated with phosphate-buffered saline [PBS]): these data suggest that clavanin A prevents the start of sepsis and thereby reduces mortality. These data suggest that clavanin A is an AMP that could improve the development of novel peptide-based strategies for the treatment of wound and sepsis infections.

scholarly journals Comparative study of the chemical composition, larvicidal, antimicrobial and cytotoxic activities of volatile oils from E. punicifolia leaves from Minas Gerais and Goiás

2021 ◽  
Vol 10 (11) ◽  
pp. e34101119354
Author(s):  
Liliane de Sousa Silva ◽  
Christiane França Martins ◽  
Fernando Yano Abrão ◽  
Camila Aline Romano ◽  
Soraia Ferreira Bezerra ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Comparative Study ◽  
Cytotoxic Activity ◽  
Larvicidal Activity ◽  
Therapeutic Potential ◽  
Volatile Oil ◽  
Minas Gerais ◽  
Cytotoxic Activities ◽  
Volatile Oils ◽  
Murine Fibroblasts

Eugenia punicifolia (Kunth) D.C., Myrtaceae, known as “pedra-ume-caá”, is popularly used in the treatment of inflammation, infections, fever, flu, diabetes, and diarrhea. This study aimed to carry out a comparative study of the chemical composition of volatile oil from E. punicifolia leaves collected in Goiás and Minas Gerais, as well as to evaluate the larvicidal activity against Aedes aegypti L3 larvae, the antimicrobial activity against bacteria, pathogenic fungi, and environmental, and cytotoxic activity to Balb 3T3 cells (murine fibroblasts). Volatile oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by Gas Chromatography Coupled to Mass Spectrometry (CG/MS). A total of 60 compounds were identified, the main components found in the leaves of Goiás being Germacrene D, bicyclogermacrene and β-longipenene and in the leaves collected in Minas Gerais they were (Z)-caryophyllene, γ-cadinene, spathulenol, caryophyllene oxide, and α-cadinol. The larvicidal effect was moderate against Ae. aegypti, with LC50 of 85.53 µg / mL for samples from Goiás and LC50 of 91.52 µg / mL for samples from Minas Gerais. Both oils showed moderate bactericidal activity against K. rhiziphyla (ATCC 9341), M. luteus (ATCC 10240), and S. aureus (ATCC 29737). The oils from Goiás (IC50 706.7 µg / mL) and Minas Gerais (IC50 160.7 µg / mL) had a lower cytotoxic concentration than the toxic action for larvae and bacteria, evidencing a safety profile and an interesting therapeutic potential, mainly concerning to volatile oil from Goiás. Therefore, the volatile oils from E. punicifolia leaves collected in Goiás and Minas Gerais that presented moderate larvicidal activity for Ae. aegypti also presented a bactericide activity and less cytotoxicity against murine fibroblasts. This is the first study of the larvicidal, antimicrobial and cytotoxic activity of volatile oils from E. punicifolia leaves.

Encapsulation of Silver Nanoparticles in Polylactic Acid or Poly(lactic-co-glycolic acid) and Their Antimicrobial and Cytotoxic Activities

2019 ◽  
Vol 19 (11) ◽  
pp. 6933-6941
Author(s):  
Daissy Julieth Paredes Guerrero ◽  
Jhon Jhamilton Artunduaga Bonilla ◽  
Claudia Cristina Ortiz López ◽  
Rodrigo Gonzalo Torres Sáez
Keyword(s):  
Silver Nanoparticles ◽  
Mammalian Cells ◽  
Metallic Nanoparticles ◽  
Pathogenic Bacteria ◽  
Polymeric Nanoparticles ◽  
Candida Guilliermondii ◽  
Cytotoxic Activities ◽  
Polymeric Nanocomposites ◽  
Murine Fibroblast ◽  
Average Size

Encapsulation with biodegradable polymers is an alternative to reduce adverse effects and improve the physicochemical properties of metallic nanoparticles. In this study, spherical polymeric nanoparticles with an average size of 200 nm loaded with silver nanoparticles (AgNPs) were obtained. The antimicrobial activity against Escherichia coli O157:H7, methicillin-resistant Staphylococcus aureus (MRSA), and yeasts as Candida albicans, Candida parapsilosis and Candida guilliermondii was determined. MIC90 values of nanocomposites were between 0.01 to 1 μg/mL, potentialized effect up to 500 times compared to free AgNPs. In addition, cytotoxic effect on 50% of murine fibroblast (CC50) was obtained at a mean concentration of 9.57 μg/mL of AgNPs (up to ~1000 times higher than MIC90). Consequently, the polymeric nanocomposites loaded with AgNPs are a potential alternative in the development of new biocide agents on Candida species and pathogenic bacteria at non-toxic concentrations for mammalian cells such as murine fibroblasts.

Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: A comparative study

2007 ◽  
Vol 45 (9) ◽  
pp. 1770-1776 ◽  
Author(s):  
Yerra Koteswara Rao ◽  
Madamanchi Geethangili ◽  
Shih-Hua Fang ◽  
Yew-Min Tzeng
Keyword(s):  
Comparative Study ◽  
Cytotoxic Activities ◽  
Naturally Occurring ◽  
Related Compounds
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