Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro

2017 ◽  
Vol 155 ◽  
pp. 323-331 ◽  
Author(s):  
Vladan Bajić ◽  
Biljana Spremo-Potparević ◽  
Lada Živković ◽  
Andrea Čabarkapa ◽  
Jelena Kotur-Stevuljević ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ascorbic Acid ◽  
Tio2 Nanoparticles ◽  
Antioxidant Properties ◽  
Surface Modified

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

scholarly journals Synthesis and Antioxidant Properties of Novel 2-(2,4-Dioxothiazolidin-5-ylidene)-Acetamides Containing 1,3,4-Thia/Oxadiazole Moieties

2021 ◽  
Vol 12 (5) ◽  
pp. 6710-6722
Keyword(s):  
Ascorbic Acid ◽  
Acetyl Chloride ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scavenging Effect ◽  
Highly Active ◽  
Ethanol Medium ◽  
Ic50 Value ◽  
Radical Scavenging Ability

A series of novel 1,3,4-thia(oxa)diazole substituted 2-(2,4-dioxothiazolidine-5-ylidene)-acetamides 3a-c, 4 and 5a-k have been synthesized following the acylation reaction of 2-amino-5-aryl-1,3,4-oxadiazoles, 5-amino-1,3,4-thiadiazole-2-thiol and it’s S-alkylated derivatives with 2-(2,4-dioxothiazolidine-5-ylidene)acetyl chloride in dioxane medium. The functionalization of compounds 3b, 3c, 5d and 5e was carried out on their N3 position under N-alkylation conditions with N-aryl-2-chloroacetamides in DMF/ethanol medium yielded the corresponding 2,4-dioxothiazolidine-3,5-diacetic acid diamides 6a-e and 7a-b. The structures of target compounds were confirmed by using 1H NMR spectroscopy and elemental analysis. The antioxidant activity evaluation in vitro of the synthesized compounds was performed by the method of scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. As a result, the highly active compound 4, namely 2-(2,4-dioxothiazolidin-5-ylidene)-N-(5-mercapto-[1,3,4]thiadiazol-2-yl)acetamide was found to be the most efficient candidate among all compounds with a radical scavenging ability of 88.9%, which was comparable that for ascorbic acid (92.7%). The experimentally calculated IC50 value of 43.1 µM for compound 4 was lower than for ascorbic acid (50.5 µM).

Phytochemical analysis and nephroprotective effect of cactus (Opuntia ficus-indica) cladodes on sodium dichromate-induced kidney injury in rats

2019 ◽  
Vol 44 (3) ◽  
pp. 239-247
Author(s):  
Mbarka Hfaiedh ◽  
Dalel Brahmi ◽  
Mohamed Nizar Zourgui ◽  
Lazhar Zourgui
Keyword(s):  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Wistar Rat ◽  
Phytochemical Analysis ◽  
Sodium Dichromate ◽  
Opuntia Ficus Indica

Environmental and occupational exposure to chromium compounds, especially hexavalent chromium, is widely recognized as potentially nephrotoxic in humans and animals. The present study aimed to assess the efficacy of cactus (Opuntia ficus-indica) against sodium dichromate-induced nephrotoxicity, oxidative stress, and genotoxicity. Cactus cladodes extract (CCE) was phytochemically studied and tested in vitro for its potential antioxidant activities. Additionally, the preventive effect of CCE against sodium dichromate-induced renal dysfunction in a Wistar rat model (24 rats) was evaluated. For this purpose, CCE at a dose of 100 mg/kg was orally administered, followed by 10 mg/kg sodium dichromate (intraperitoneal injection). After 40 days of treatment, the rats were sacrificed, and the kidneys were excised for histological, lipid peroxidation, and antioxidant enzyme analyses. The phenol, flavonoid, tannin, ascorbic acid, and carotenoid contents of CCE were considered to be important. Our analyses showed that 1 mL of CCE was equivalent to 982.5 ± 1.79 μg of gallic acid, 294.37 ± 0.84 μg of rutin, 234.78 ± 0.24 μg of catechin, 204.34 ± 1.53 μg of ascorbic acid, and 3.14 ± 0.51 μg of β-carotene. In vivo, pretreatment with CCE was found to provide significant protection against sodium dichromate-induced nephrotoxicity by inhibiting lipid peroxidation, preserving normal antioxidant activities, and protecting renal tissues from lesions and DNA damage. The nephroprotective potential of CCE against sodium dichromate toxicity might be due to its antioxidant properties.

2‐Amino‐5‐arylazothiazole‐Based Derivatives: In Vitro Cytotoxicity, Antioxidant Properties, and Bleomycin‐Dependent DNA Damage

2019 ◽  
Vol 4 (19) ◽  
pp. 5570-5576 ◽  
Author(s):  
Khalid M. Al‐Anazi ◽  
Ahmed H. Mahmoud ◽  
Mohammad AbulFarah ◽  
Ahmed A. Allam ◽  
Moustafa M. G. Fouda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Properties ◽  
In Vitro Cytotoxicity

scholarly journals In vitro antioxidant activity of meniran (Phyllantus urinaria) functional drink in human low density lipoprotein (LDL)

2021 ◽  
Vol 913 (1) ◽  
pp. 012093
Author(s):  
U Fitrotin ◽  
N Hilmiati ◽  
Mardiana ◽  
Y Triguna ◽  
A Surahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Ldl Oxidation ◽  
Functional Drink

Abstract Preparation process for meniran (Phillantus urinaria) functional drink (MFD) influences its antioxidant activity. This research aims to understand the phenolic content, DPPH Radical Scavenging Activity (RSA), and LDL oxidation of MFD through various preparation processes. Those preparation processes included soaking fresh meniran (SFM), boiling fresh meniran for 5 minutes (BFM5’), boiling fresh meniran for 10 minutes (BFM10’), and soaking dried meniran (DM). The phenolic content was determined with Folin–Ciocalteu, antioxidant activity was assessed using DPPH and TBARS assay with LDL as the oxidation substrate. An antioxidant references in this research used ascorbic acid. The phenolic content in methods of SFM, BFM5’, BFM10’ and DM were 122±0.022, 182±0.043, 192 ±0.03, and 117 ±0.019 mg GAE/g of meniran respectively. Meanwhile, the DPPH RSA of SFM, BFM5’, BFM10’ and DM accounted for 82.18±0.35, 86.19±0.53, 86.75±0.64 and 69.96% respectively. As comparison, the DPPH RSA of ascorbic acid 50 ppm is 75.65±0.82%. At the same time the optimum inhibition of TBARS formation from BFM5’ and BFM10’ methods were 45.83 % and 48.66%, with MDA concentration in human LDL accounted for 38.30±2.39 and 36.30±1.82 nmol MDA/mg protein, respectively. As comparison, MDA concentration in human LDL added with ascorbic acid 25 ppm accounted for 41.35±2.41 nmol MDA/mg protein. In contrast, the control human LDL was 70.70±2.35 nmol MDA/mg protein. This study concludes that the BFM5’ and BFM10’ methods showed the highest antioxidant properties compared to other methods. All methods showed that MFD extract in concentration more than 25 ppm increased the concentration of MDA in human LDL. Therefore, to produce meniran functional drink in optimum antioxidant properties is best by using BFM5’ and BFM10’ preparation methods in meniran concentration of not more than 25 ppm.

scholarly journals In vitro cytotoxicity and antibiotic application of green route surface modified ferromagnetic TiO2 nanoparticles

RSC Advances ◽  
2019 ◽  
Vol 9 (23) ◽  
pp. 13254-13262 ◽  
Author(s):  
A. K. M. Atique Ullah ◽  
A. N. Tamanna ◽  
A. Hossain ◽  
M. Akter ◽  
M. F. Kabir ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Tio2 Nanoparticles ◽  
Leaf Extract ◽  
In Vitro Cytotoxicity ◽  
Artocarpus Heterophyllus ◽  
High Antibacterial Activity ◽  
Green Route ◽  
Surface Modified

Artocarpus heterophyllus leaf extract mediated green synthesized TiO2 nanoparticles exhibit less toxicity with high antibacterial activity.

scholarly journals The Effect of an Edible Coating with Tomato Oily Extract on the Physicochemical and Antioxidant Properties of Garambullo (Myrtillocactus geometrizans) Fruits

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 248 ◽  
Author(s):  
César López-Palestina ◽  
César Aguirre-Mancilla ◽  
Juan Raya-Pérez ◽  
Juan Ramírez-Pimentel ◽  
Jorge Gutiérrez-Tlahque ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Titratable Acidity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Trolox Equivalent ◽  
Gelatin Coating ◽  
Reduced Water ◽  
Capacity Method

The Myrtillocactus geometrizans (Mart. ex. Pfeiff.) fruit, locally called garambullo, is an important source of bioactive compounds, mainly betalains, polyphenols, and ascorbic acid. However, information on the application of post-harvest technologies that prolong the shelf life of the fruits is still insufficient. The objective of the present research was to evaluate the effect of a gelatin coating incorporating 0%, 1%, or 3% tomato oily extract (TOE), compared to a control (without coating), on the physicochemical and antioxidant properties of garambullo fruits stored for 15 days at 5 °C. The gelatin coatings with TOE significantly (p ≤ 0.05) delayed changes in weight loss, brix degrees, titratable acidity and pH, compared to the control during storage. Fruits coated with TOE-gelatin had a higher content of betalains, ascorbic acid, total phenols, and flavonoids. The results showed that a higher concentration of phytochemicals increased antioxidant activity in vitro; the maximum values found for Trolox equivalents per kg of fresh weight were 10.46 and 17.65 mM for the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and Trolox Equivalent Antioxidant Capacity Method (TEAC) tests, respectively. The gelatin coating with TOE-3% (COTE-3%) reduced water loss by 1.66 times, compared to the control. In addition, the fruits covered with COTE-3% showed the highest concentration of bioactive compounds during storage.

E.P.R. Spectroscopic Studies of the Reactions of Chromium(VI) with L-Ascorbic Acid in Buffer Solutions: Implications for Understanding the Roles of Chromium(V) and Chromium(IV) Species and the Buffer Dependences of In Vitro DNA Damage

2000 ◽  
Vol 53 (1) ◽  
pp. 7 ◽  
Author(s):  
Lianbo Zhang ◽  
Peter A. Lay
Keyword(s):  
Dna Damage ◽  
Ascorbic Acid ◽  
Spectroscopic Studies ◽  
Mixed Ligand ◽  
Peroxo Complexes ◽  
Buffer Solutions ◽  
Chromium Vi ◽  
Dna Strand

The reaction of chromium(VI) with L-ascorbic acid (AsA) in buffer solutions was investigated by e.p.r. spectroscopy. Chromium(V)/ascorbate complexes with signals at giso = 1.9791 and chromium(VI)/ascorbate/peroxo complexes with signals at giso = 1.9819 and giso = 1.9824 were observed in all buffers. New signals at giso values of 1.9765 and 1.9781 were observed in Tris–HCl [tris(hydroxymethyl)aminomethane hydrochloride] buffer and were assigned to a mixed-ligand ascorbate/Tris complex of chromium(V), [CrO(ascorbate)(Tris)]2– , and a Tris/peroxo species, [CrO(O2)(Tris)]2– , respectively. The speciation of the e.p.r.-active chromium(V) complexes detected from solutions with other buffers, such as HEPES, cacodylate and phosphate, was not influenced by the buffer type. Preincubation of catalase with ascorbate solutions inhibited the formation of the chromium(V) peroxo species in all buffers. Manganese(II) reduces the chromium(V) species produced in the reaction, which has shown that it is inappropriate to use manganeses(II) as a selective reagent for monitoring the concentrations of chromium(IV) in such reactions. In particular, manganeses(II) reacts more efficiently with the chromium(V) species that are most damaging to DNA in vitro, viz., the mixed-ligand chromium(V)/ascorbate/peroxo complexes. The correlation of the present results with those of in vitro DNA damage experiments reported in the literature has revealed that the chromium(V)/ascorbate/peroxo species are the major species responsible for the in vitro DNA strand breaks in all of the buffer systems. These species are not expected to be as important in vivo.

scholarly journals PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIOXIDANT STATUS OF CLERODENDRUM VOLUBILE (AN INDIGENOUS MEDICINAL PLANT)

2016 ◽  
Vol 2 (2) ◽  
pp. 77 ◽  
Author(s):  
Tomisin Happy Ogunwa ◽  
Tolulope Tosin Adeyelu ◽  
Rotimi Yemi Fasimoye ◽  
Mary Bose Oyewale ◽  
Taiwo Ademola Ademoye ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Research Work ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Therapeutic Effects ◽  
Dpph Radical

ABSTRACTClerodenrum volubile is an important locally used medicinal plant. It is one of the essential herbs nature has provided for mankind to be consumed as spices, vegetable and also used in the treatment of diseases. Qualitative phytoconstituent screening of the plant revealed the presence of steroids, flavonoids, tannins, saponins, and phenolic compound while chalcones, alkaloid and anthraquinone were absent. Quantitative phytochemical evaluation showed values of 8.29±1.26mg/g rutin equivalent for flavonoids, 3.53±0.05mg/g gallic acid equivalent for phenol, 3.97±0.03mg/g tannic acid equivalent for tannins and 13.67±1.27% for saponin per dry weight. As it is imperative to extend research work on therapeutic effects of the arsenal of plants nature has given to us in Africa so as to obtain a cure for various diseases attacking human’s health, antioxidant properties of the plant was evaluated. Antioxidant models used include iron chelating, DPPH radical, superoxide ion, hydrogen peroxide, ABTS radical, hydroxyl radical scavenging activities and ferric ion reducing properties. The obtained IC50 values against DPPH radical were 141.342 and 120.349µg/mL for Clerodendrum volubile and trolox respectively. The chelating effect of the plant extract at 50% inhibition was close to that of ascorbic acid (standard) with 134.34 and 131.19µg/mL concentration respectively. Overall, the aqueous extract of the plant showed antioxidant potential which was close to the effects exerted by known standard antioxidants (ascorbic acid, trolox and EDTA). The plant could hence provide natural antioxidants which are needed to combat numerous free radical-mediated diseases and complications such as aging, cancer, atherosclerosis, which are linked with oxidative stress. 

scholarly journals Therapeutic Management of Pulmonary Tuberculosis by Mannosylated Chitosan Ascorbate Microspheres: Preparation and Characterization

2019 ◽  
Vol 9 (3) ◽  
pp. 13-25
Author(s):  
Archana Bagre ◽  
Narendra Kumar Lariya ◽  
Mohan Lal Kori
Keyword(s):  
Ascorbic Acid ◽  
Pulmonary Tuberculosis ◽  
Alveolar Macrophages ◽  
Antioxidant Properties ◽  
A549 Cells ◽  
Lung Epithelial Cells ◽  
Scanning Calorimetry ◽  
Aerodynamic Properties

Objective: In this study, biodegradable Chitosan Ascorbate Microsphere (CAMs) and mannosylated chitosan ascorbate microsphere (m-CAMs) prepared for targeting towards alveolar macrophages to treatment of pulmonary tuberculosis.  Significance: Ascorbic acid is an antioxidant and reported killing effect on mycobacterium by induces fenton reaction. This study enlightens the possible benefits of adding antioxidant properties of ascorbic acid with chitosan microsphere to an anti-tuberculosis regimen and mannosylation of microsphere significantly induce the targetability of antitubercular drug to alveolar macrophages. Methods: CAMs prepared by firstly salification of chitosan by ascorbic acid then ionic gelation with STPP and m-CAMs prepared by incubation method and purified for further studies. The physicochemical, in vitro and in vivo characterizations of both formulations were carried out. Results: The size of microspheres (both CAMs and m-CAMs) were found to be in range of 3.40-4.81µm. Evident changes were observed in crystallinity and structure of both carrier systems and depicted by Fourier transform infrared (FTIR), Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) studies. In vitro lung deposition study of microspheres showed favourable aerodynamic properties for deep lung delivery (MMAD 2.0- 3.8 μm) and, thus, show potential for an application as inhalable tuberculosis therapy. The drug release showed the biphasic pattern of release, i.e., initial burst (30-45% up to 8 h) followed by a slower sustained release pattern (more than 80% up to 72 h) in both simulated lung fluids. Optimized formulations exhibited lower cytotoxicity and bio distribution studies demonstrated the efficiency of m-CAMs for spatial delivery of INH to alveolar tissues. CAMs and m-CAMs evidenced minor cytotoxicity on lung epithelial cells (A549 cell lines). Conclusion: m-CAMs thus has a promising potential to be explore as an effective carrier system for delivery of antitubercular drugs regimen. Key words: Targetability, alveolar macrophage, lung cancer A549 cells

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