A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test

2018 ◽  
Vol 91 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Lucyna Holec-Gąsior ◽  
Bartłomiej Ferra ◽  
Justyna Czechowska ◽  
Illia E. Serdiuk ◽  
Karol Krzymiński
Keyword(s):  
Elisa Test ◽  
Conventional Elisa ◽  
Chemiluminescent Immunoassay ◽  
Acridinium Ester

Competitive Chemiluminescent Immunoassay for Estradiol Using anN-Functionalized Acridinium Ester

1996 ◽  
Vol 11 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Hiroshi Sato ◽  
Hiroshi Mochizuki ◽  
Yuki Tomita ◽  
Toshio Izako ◽  
Naofumi Sato ◽  
...  
Keyword(s):  
Chemiluminescent Immunoassay ◽  
Acridinium Ester

A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled

2014 ◽  
Vol 707 ◽  
pp. 7-11
Author(s):  
Fan Fan Yang ◽  
Li Xin Zhu ◽  
Long Xu ◽  
Ren Rong Liu ◽  
Yan Fan ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Inhibitory Concentration ◽  
Limit Of Detection ◽  
Uv Spectrum ◽  
Chemiluminescence Immunoassay ◽  
Highly Sensitive ◽  
Chemiluminescent Immunoassay ◽  
Inhibition Curve ◽  
Sensitive Method ◽  
Acridinium Ester

A novel chemiluminescence immunoassay (CLIA) of Bisphenol A (BPA) with the acridinium ester of NSP-SA-NHS-labeled has been developed. In this study, BVA and NSP-SA-NHS had been coupled with BSA, the UV spectrum results indicated the conjugates which were successfully synthesized. Basing on these luminescence data the inhibition curve of BPA was established, then the linear arrang of the curve was between 0.4 ng/ml and 5 ng/ml, the 50% inhibitory concentration (IC50) was 2.3ng/ml, the lowest limit of detection was 0.1ng/ml, which showed it’s an efficient and highly sensitive method.

One-step chemiluminescent immunoassay of free thyroxin with acridinium-ester-labeled thyroxin evaluated and compared with a two-step radioimmunoassay.

1988 ◽  
Vol 34 (12) ◽  
pp. 2556-2560 ◽  
Author(s):  
J Y Bounaud ◽  
M P Bounaud ◽  
F Begon
Keyword(s):  
Confidence Interval ◽  
Significant Interaction ◽  
Binding Proteins ◽  
Clinical Status ◽  
One Step ◽  
Chemiluminescent Immunoassay ◽  
Therapy Treatment ◽  
Acridinium Ester

Abstract This new one-step chemiluminescent immunoassay of free thyroxin (FT4) involving a thyroxin-immunoglobulin conjugate labeled with acridinium ester (Magic Lite System; Ciba Corning Diagnostics Corp., Medfield, MA) is rapid (one 1-h incubation), requires two calibrators per run, and takes 10 s per sample for the quantification step. Analytical performances were excellent: within- and between-run CVs of less than 10% in the working range, no significant effect of hemolysis, bilirubin, or lipemia, and no significant interaction between the conjugate and the thyroxin-binding proteins. Magic Lite results (y) correlated well with those obtained by the Sclavo (x) two-step radioimmunoassay (Sclavo, Siena, Italy): y = 1.35x + 1.32 (r = 0.94, n = 267, P less than 0.001, Sxy = 6.29). Clinical sensitivities (diagnostic efficiencies) for hypothyroidism and hyperthyroidism were 0.91 and 0.98 for normal interval limits of 12 and 21.5 pmol/L (95% confidence interval). Magic Lite results in situations where patient therapy, treatment, or unusual conditions can result in a lack of correlation between the clinical status and the FT4 values were qualitatively the same as those obtained by the Sclavo assay.

Evaluation of D-dimer ELISA Test in Elderly Patients with Suspected Pulmonary Embolism

1998 ◽  
Vol 79 (01) ◽  
pp. 38-41 ◽  
Author(s):  
Brigitte Tardy-Poncet ◽  
Alain Viallon ◽  
Pierre Lafond ◽  
Y. Page ◽  
Christophe Venet ◽  
...  
Keyword(s):  
Predictive Value ◽  
High Probability ◽  
Elisa Test ◽  
Suspected Pulmonary Embolism ◽  
Duplex Scan ◽  
Vein Thrombosis ◽  
Conventional Elisa ◽  
Deep Vein ◽  
D Dimer

SummaryStudy Objective: To determine the clinical usefulness of D-dimer ELISA test in elderly patients with clinically suspected pulmonary embolism (PE). Design: Prospective cohort study. Patients: Ninety-six consecutive outpatients older than 70 years with a duration of symptoms shorter than one week and without metastatic cancer or recent surgery, trauma, infection, stroke, myocardial infarction, deep vein thrombosis (DVT) or PE, or treatment with curative doses of heparin or oral anticoagulant. Intervention: All patients underwent at least ventilation/perfusion scan and bilateral ultrasonic duplex scan and a blood sample collection within 24 hours of admission. When necessary a pulmonary angiography and/or a bilateral venography were also performed. Patients were classified as follows: (1) PE-positive; positive angiography or high probability V/Q scan and deep vein thrombosis (proven either by venography or by ultrasonic duplex scan) or non high probability V/Q scan and either DVT (proven at presentation by venography or by ultrasonic duplex scan) or symptomatic thromboembolic event within 3 months of follow-up; or (2) PE-negative; normal V/Q scan or normal angiography or non high probability V/Q scan and either negative ultrasonic duplex scan or normal venography and low clinical probability and absence of symptomatic thromboembolism within 3 months of follow-up. D-dimer measurements were performed using both a conventional and a single semi-quantitative ELISA test (Asserachrom D-di, Instant I.A.D-dimer). Results: Using a cutoff value of 500 ng/ml, the conventional ELISA D-dimer test showed a sensitivity and a negative predictive value of 100% with poor specificity and positive predictive value of 14.3% and 45.5% respectively. The new rapid semi-quantitative D-dimer test displays worse results with sensitivity, negative predictive value, specificity and positive predictive value of 92.3%, 82.4%, 25% and 46% respectively. Conclusion: In a geriatric population, conventional ELISA D-dimer is a good marker to exclude PE but, due to the comorbid conditions, only a few patients presented with D-dimer values less than 500 ng/ml.

scholarly journals Advantages of the rapid HIV-1 test in occupational accidents with potentially contaminated material among health workers

2001 ◽  
Vol 43 (4) ◽  
pp. 199-201 ◽  
Author(s):  
Alcyone Artioli MACHADO ◽  
Roberto MARTINEZ ◽  
Amira Aparecida HAIKAL ◽  
Marta Cristina Vicente RODRIGUES DA SILVA
Keyword(s):  
Health Workers ◽  
Rapid Test ◽  
Elisa Test ◽  
University Hospital ◽  
Occupational Accidents ◽  
Antiretroviral Agents ◽  
Conventional Elisa ◽  
Prophylactic Use ◽  
The University ◽  
Hiv 1

In occupational accidents involving health professionals handling potentially contaminated material, the decision to start or to continue prophylactic medication against infection by Human Immunodeficiency Virus (HIV) has been based on the ELISA test applied to a blood sample from the source patient. In order to rationalize the prophylactic use of antiretroviral agents, a rapid serologic diagnostic test of HIV infection was tested by the enzymatic immunoabsorption method (SUDS HIV 1+2, MUREX®) and compared to conventional ELISA (Abbott HIV-1/ HIV-2 3rd Generation plus EIA®). A total of 592 cases of occupational accidents were recorded at the University Hospital of Ribeirão Preto from July 1998 to April 1999. Of these, 109 were simultaneously evaluated by the rapid test and by ELISA HIV. The rapid test was positive in three cases and was confirmed by ELISA and in one the result was inconclusive and later found to be negative by ELISA. In the 106 accidents in which the rapid test was negative no prophylactic medication was instituted, with an estimated reduction in costs of US$ 2,889.35. In addition to this advantage, the good correlation of the rapid test with ELISA, the shorter duration of stress and the absence of exposure of the health worker to the adverse effects of antiretroviral agents suggest the adoption of this test in Programs of Attention to Accidents with Potentially Contaminated Material.

Automated Latex Agglutination and ELISA Testing Yield Equivalent D-Dimer Results in Patients with Recent Myocardial Infarction

1999 ◽  
Vol 82 (11) ◽  
pp. 1412-1416 ◽  
Author(s):  
Wojciech Zareba ◽  
John Horan ◽  
Arthur Moss ◽  
Joel Kanouse ◽  
◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Elisa Test ◽  
Mean Value ◽  
Latex Agglutination ◽  
Test Results ◽  
Coronary Death ◽  
Elisa Testing ◽  
Strong Linear Correlation ◽  
Highly Correlated ◽  
D Dimer

SummaryOur previous prospective study of post-infarction patients described a strong and significant association of increased plasma D-dimer concentrations in those who experienced a subsequent coronary death or non-fatal myocardial infarction. In the present study, we compare results on stored plasma obtained two months after the index myocardial infarction from 1,038 patients of this trial, using a simple automated latex agglutination (LA) assay in parallel with the standard ELISA test. Results show a somewhat higher mean value for the LA assay (702 ± 1092 vs. 638 ± 986 ng/ml, p = 0.0002), a strong linear correlation of the two assays (r = 0.86) and 88% agreement for values below 500 ng/ml by the ELISA test. D-dimer concentrations determined by each assay were highly correlated in patients with subsequent coronary artery events (p = 0.93) and quartile values for both the LA and ELISA were equally predictive of such events (p = 0.003 and p = 0.001, respectively). This is the first demonstration that a latex agglutination assay for D-dimer can be used to assess the prognostic risk of recurrent coronary thrombotic disease after myocardial infarction

Immunodetection of Ostertagia ostertagi in Cattle Milk Samples in Wasit Province

2017 ◽  
Vol 1 (28) ◽  
pp. 637-648
Author(s):  
Abbas Hassan Khlaty Al-Sray
Keyword(s):  
Milk Production ◽  
Herd Size ◽  
Elisa Test ◽  
Production Factor ◽  
Size Factor ◽  
Ostertagia Ostertagi ◽  
Milk Samples ◽  
Management Group ◽  
Breed Group ◽  
Age Factor

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ³18 liters/day group, 14.29 % for ³10-18 liters/day group, and 25 % for < 10 liters/day group; in age factor, 13.41% for ³3-6 years group, and 14.75% for >6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for <25 (cow/herd) group, and 17.78% for ³ 25 (cow/herd) group. Statistically, the significant differences (P£ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.

MONITORING STUDIES OF ARBOVIRUS INFECTIONS TRANSMITTED BY MOSQUITOES ON THE TERRITORY OF THE VOLGOGRAD REGION

2019 ◽  
pp. 60-66
Author(s):  
E.V. Molchanova ◽  
D.N. Luchinin ◽  
A.O. Negodenko ◽  
D.R. Prilepskaya ◽  
N.V. Boroday ◽  
...  
Keyword(s):  
Virus Antigen ◽  
Elisa Test ◽  
Serum Samples ◽  
Blood Sucking ◽  
Tick Borne Encephalitis ◽  
Volgograd Region ◽  
Two Samples ◽  
Probable Presence ◽  
California Serogroup ◽  
Tick Borne Encephalitis Virus

The paper presents data from the monitoring studies’ results of arbovirus infections transmitted by mosquitoes in the Volgograd region. West Nile virus antigen (WNV) in 9 samples, Tahyna virus in one sample, Batai virus in two samples were detected in the study of 110 samples of field material (blood-sucking mosquitoes) by ELISA test. Antibodies to WNV in 16.58 percent of the samples, to tick-borne encephalitis virus in 1.08 percent, to viruses of the California serogroup and Ukuniemi in 1.09 percent, to the virus Sindbis in 2.17 percent were detected as a result of the study of blood serum samples from donors in the Volgograd region. Thus, we obtained data on the probable presence of the Batai, Sindbis, Ukuniemi and Californian serogroup viruses along with the circulation of WNV on the territory of the Volgograd region.

Sign in / Sign up

Export Citation Format

Share Document