The impact of biomechanics on corneal endothelium tissue engineering

Damaged or disabled tissue is life-threatening due to the lack of proper treatment. Many conventional transplantation methods like autograft, iso-graft and allograft are in existence for ages, but they are not sufficient to treat all types of tissue or organ damages. Stem cells, with their unique capabilities like self-renewal and differentiate into various cell types, can be a potential strategy for tissue regeneration. However, the challenges like reproducibility, uncontrolled propagation and differentiation, isolation of specific kinds of cell and tumorigenic nature made these stem cells away from clinical application. Today, various types of stem cells like embryonic, fetal or gestational tissue, mesenchymal and induced-pluripotent stem cells are under investigation for their clinical application. Tissue engineering helps in configuring the stem cells to develop into a desired viable tissue, to use them clinically as a substitute for the conventional method. The use of stem cell-derived Extracellular Vesicles (EVs) is being studied to replace the stem cells, which decreases the immunological complications associated with the direct administration of stem cells. Tissue engineering also investigates various biomaterials to use clinically, either to replace the bones or as a scaffold to support the growth of stemcells/ tissue. Depending upon the need, there are various biomaterials like bio-ceramics, natural and synthetic biodegradable polymers to support replacement or regeneration of tissue. Like the other fields of science, tissue engineering is also incorporating the nanotechnology to develop nano-scaffolds to provide and support the growth of stem cells with an environment mimicking the Extracellular matrix (ECM) of the desired tissue. Tissue engineering is also used in the modulation of the immune system by using patient-specific Mesenchymal Stem Cells (MSCs) and by modifying the physical features of scaffolds that may provoke the immune system. This review describes the use of various stem cells, biomaterials and the impact of nanotechnology in regenerative medicine.

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Information and scientific impact of Advanced Therapies in the age of mass media: an Altmetrics-based analysis of Tissue Engineering. (Preprint)

10.2196/preprints.25394 ◽

2020 ◽

Author(s):

Antonio Santisteban ◽

Julia Moran ◽

Miguel Ángel Martín Piedra ◽

Antonio Campos Muñoz ◽

José Antonio Moral Muñoz ◽

...

Keyword(s):

Tissue Engineering ◽

Mass Media ◽

Prediction Equation ◽

Factorial Analysis ◽

Online Media ◽

Research Groups ◽

Academic Institutions ◽

Scientific Impact ◽

Online Platforms ◽

The Impact

BACKGROUND Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-staged organs. Its development has taken placed since the last decade of the 20th century, entailing a clinical revolution. In this sense, TE research groups have worked and shared relevant information in the mass media era. Thus, it would be interesting to study the online dimension of TE research and to compare it with traditional measures of scientific impact. OBJECTIVE To evaluate TE online dimension from 2012 to 2018 by using metadata obtained from the Web of Science (WoS) and Altmetrics and to develop a prediction equation for the impact of TE documents from Almetrics scores. METHODS We have analyzed 23,719 TE documents through descriptive and statistical methods. First, TE temporal evolution was exposed for WoS and fifteen online platforms (News, Blogs, Policy, Twitter, Patents, Peer review, Weibo, Facebook, Wikipedia, Google, Reddit, F1000, Q&A, Video and Mendeley readers). The 10 most-cited TE original articles were ranked according to WoS citations and the Altmetric Attention Score. Second, in order to better comprehend TE online framework, a correlation and factorial analysis were performed based on the suitable results previously obtained for the Bartlett Sphericity and Kaiser-Meyer-Olkin tests. Finally, the liner regression model was applied to elucidate the relation between academy and online media and to construct a prediction equation for TE from Altmetrics data. RESULTS TE dynamic shows an upward trend in WoS citations, Twitter, Mendeley Readers and Altmetric Scores. However, WoS and Altmetrics rankings for the most cited documents clearly differs. When compared, the best correlation results were obtained for Mendeley readers and WoS (ρ=0.71). In addition, the factorial analysis identified six factors that could explain the previously observed differences between TE academy, and the online platforms evaluated. At this point, the mathematical model constructed is able to predict and explain more than the 40% of TE WoS citations from Altmetrics scores. CONCLUSIONS The scientific information related to the construction of bioartificial tissues increasingly reaches society through different online media. Because of the focus of TE research importantly differs when the academic institutions and online platforms are compared, it could be stated that basic and clinical research groups, academic institutions and health politicians should take it into account in a coordinated effort oriented to the design and implementation of adequate strategies for information diffusion and population health education.

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Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

Current Directions in Biomedical Engineering ◽

10.1515/cdbme-2020-3106 ◽

2020 ◽

Vol 6 (3) ◽

pp. 410-413

Author(s):

Petra J. Kluger ◽

Svenja Nellinger ◽

Simon Heine ◽

Ann-Cathrin Volz

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Adipogenic Differentiation ◽

Cellular Activity ◽

Adipose Tissue Engineering ◽

Physical Support ◽

Supporting Effect ◽

Brdu Assay ◽

The Impact

AbstractThe extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.

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Design and Mechanical Properties Verification of Gradient Voronoi Scaffold for Bone Tissue Engineering

Micromachines ◽

10.3390/mi12060664 ◽

2021 ◽

Vol 12 (6) ◽

pp. 664

Author(s):

Haiyuan Zhao ◽

Yafeng Han ◽

Chen Pan ◽

Ding Yang ◽

Haotian Wang ◽

...

Keyword(s):

Mechanical Property ◽

Tissue Engineering ◽

Elastic Modulus ◽

Porous Structure ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Voronoi Tessellation ◽

Impact Resistance ◽

Natural Bone ◽

The Impact

In order to obtain scaffold that can meet the therapeutic effect, researchers have carried out research on irregular porous structures. However, there are deficiencies in the design method of accurately controlling the apparent elastic modulus of the structure at present. Natural bone has a gradient porous structure. However, there are few studies on the mechanical property advantages of gradient bionic bone scaffold. In this paper, an improved method based on Voronoi-tessellation is proposed. The method can get controllable gradient scaffolds to fit the modulus of natural bone, and accurately control the apparent elastic modulus of porous structure, which is conducive to improving the stress shielding. To verify the designed structure can be fabricated by additive manufacturing, several designed models are obtained by SLM and EBM. Through finite element analysis (FEA), it is verified that the irregular porous structure based on Voronoi-tessellation is more stable than the traditional regular porous structure of the same structure volume, the same pore number and the same material. Furthermore, it is verified that the gradient irregular structure has a better stability than the non-gradient structure. An experiment is conducted successfully to verify the stability performance got by FEA. In addition, a dynamic impact FEA is also performed to simulate impact resistance. The result shows that the impact resistance of the regular porous structure, the irregular porous structure and the gradient irregular porous structure becomes better in turn. The mechanical property verification provides a theoretical basis for the structural design of gradient irregular porous bone tissue engineering scaffolds.

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Vascularization Potential of Electrospun Poly(L-Lactide-co-Caprolactone) Scaffold: The Impact for Tissue Engineering

Medical Science Monitor ◽

10.12659/msm.899659 ◽

2017 ◽

Vol 23 ◽

pp. 1540-1551 ◽

Cited By ~ 6

Author(s):

Arkadiusz Jundziłł ◽

Marta Pokrywczyńska ◽

Jan Adamowicz ◽

Tomasz Kowalczyk ◽

Maciej Nowacki ◽

...

Keyword(s):

Tissue Engineering ◽

The Impact

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Environmental Impact for 3D Bone Tissue Engineering Scaffolds Life Cycle: An Assessment

Biointerface Research in Applied Chemistry ◽

10.33263/briac125.65046515 ◽

2021 ◽

Vol 12 (5) ◽

pp. 6504-6515

Keyword(s):

Tissue Engineering ◽

Life Cycle Assessment ◽

Life Cycle ◽

Environmental Impact ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Environmental Impacts ◽

Tissue Engineering Scaffolds ◽

Industrial Soil ◽

The Impact

With the development of additive manufacturing technology, 3D bone tissue engineering scaffolds have evolved. Bone tissue engineering is one of the techniques for repairing bone abnormalities caused by a variety of circumstances, such as injuries or the need to support damaged sections. Many bits of research have gone towards developing 3D bone tissue engineering scaffolds all across the world. The assessment of the environmental impact, on the other hand, has received less attention. As a result, the focus of this study is on developing a life cycle assessment (LCA) model for 3D bone tissue engineering scaffolds and evaluating potential environmental impacts. One of the methodologies to evaluating a complete environmental impact assessment is life cycle assessment (LCA). The cradle-to-grave method will be used in this study, and GaBi software was used to create the analysis for this study. Previous research on 3D bone tissue engineering fabrication employing poly(ethylene glycol) diacrylate (PEGDA) soaked in dimethyl sulfoxide (DMSO), and diphenyl (2,4,6-trimethylbenzoyl) phosphine oxide (TPO) as a photoinitiator will be reviewed. Meanwhile, digital light processing (DLP) 3D printing is employed as the production technique. The GaBi program and the LCA model developed to highlight the potential environmental impact. This study shows how the input and output of LCA of 3D bone tissue engineering scaffolds might contribute to environmental issues such as air, freshwater, saltwater, and industrial soil emissions. The emission contributing to potential environmental impacts comes from life cycle input, electricity and transportation consumption, manufacturing process, and material resources. The results from this research can be used as an indicator for the researcher to take the impact of the development of 3D bone tissue engineering on the environment seriously.

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Cerebrospinal Fluid Pulsation Stress Promotes the Angiogenesis of Tissue-Engineered Laminae

Stem Cells International ◽

10.1155/2020/8026362 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Linli Li ◽

Yiqun He ◽

Han Tang ◽

Wei Mao ◽

Haofei Ni ◽

...

Keyword(s):

Tissue Engineering ◽

Cerebrospinal Fluid ◽

Engineering Technology ◽

Expression Levels ◽

Fluid Pulsation ◽

Endothelial Growth Factor ◽

The Impact

Background. Angiogenesis is a prerequisite step to achieve the success of bone regeneration by tissue engineering technology. Previous studies have shown the role of cerebrospinal fluid pulsation (CSFP) stress in the reconstruction of tissue-engineered laminae. In this study, we investigated the role of CSFP stress in the angiogenesis of tissue-engineered laminae. Methods. For the in vitro study, a CSFP bioreactor was used to investigate the impact of CSFP stress on the osteogenic mesenchymal stem cells (MSCs). For the in vivo study, forty-eight New Zealand rabbits were randomly divided into the CSFP group and the Non-CSFP group. Tissue-engineered laminae (TEL) was made by hydroxyapatite-collagen I scaffold and osteogenic MSCs and then implanted into the lamina defect in the two groups. The angiogenic and osteogenic abilities of newborn laminae were examined with histological staining, qRT-PCR, and radiological analysis. Results. The in vitro study showed that CSFP stress could promote the vascular endothelial growth factor A (VEGF-A) expression levels of osteogenic MSCs. In the animal study, the expression levels of angiogenic markers in the CSFP group were higher than those in the Non-CSFP group; moreover, in the CSFP group, their expression levels on the dura mater surface, which are closer to the CSFP stress stimulation, were also higher than those on the paraspinal muscle surface. The expression levels of osteogenic markers in the CSFP group were also higher than those in the Non-CSFP group. Conclusion. CSFP stress could promote the angiogenic ability of osteogenic MSCs and thus promote the angiogenesis of tissue-engineered laminae. The pretreatment of osteogenic MSC with a CSFP bioreactor may have important implications for vertebral lamina reconstruction with a tissue engineering technique.

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Review on the Impact of Polyols on the Properties of Bio-Based Polyesters

Polymers ◽

10.3390/polym12122969 ◽

2020 ◽

Vol 12 (12) ◽

pp. 2969

Author(s):

Kening Lang ◽

Regina J. Sánchez-Leija ◽

Richard A. Gross ◽

Robert J. Linhardt

Keyword(s):

Tissue Engineering ◽

Biomedical Applications ◽

Molecular Level ◽

Potential Utility ◽

Reaction Conditions ◽

Wide Range ◽

Soft Tissue Engineering ◽

The Impact ◽

Optimal Reaction

Bio-based polyol polyesters are biodegradable elastomers having potential utility in soft tissue engineering. This class of polymers can serve a wide range of biomedical applications. Materials based on these polymers are inherently susceptible to degradation during the period of implantation. Factors that influence the physicochemical properties of polyol polyesters might be useful in achieving a balance between durability and biodegradability. The characterization of these polyol polyesters, together with recent comparative studies involving creative synthesis, mechanical testing, and degradation, have revealed many of their molecular-level differences. The impact of the polyol component on the properties of these bio-based polyesters and the optimal reaction conditions for their synthesis are only now beginning to be resolved. This review describes our current understanding of polyol polyester structural properties as well as a discussion of the more commonly used polyol monomers.

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